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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences e-NERS e-CliniC Jurnal e-Biomedik JURNAL BIOMEDIK JURNAL KEDOKTERAN KOMUNITAS DAN TROPIK Jurnal Keperawatan YARSI Medical Journal Jurnal Promotif Preventif Medical Scope Journal (MSJ) KESANS : International Journal of Health and Science eJournal Kedokteran Indonesia Grimsa Journal of Science Engineering and Technology Jurnal Inovasi Global
Tuda, Josef Sem Berth
Universitas Sam Ratulangi
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences

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Journal : JURNAL BIOMEDIK

 1 
KADAR TUMOR NECROSIS FACTOR – α PADA SERUM PENDERITA MALARIA DENGAN GEJALA DEMAM Thiosanto, Monica; Bernadus, Janno; Tuda, Josef S. B.
Jurnal Biomedik : JBM Vol 6, No 1 (2014): JURNAL BIOMEDIK : JBM Maret 2014
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/jbm.6.1.2014.4162

Abstract

Abstract: Malaria is classified as one of the infectious diseases that is very dangerous and can cause death. It is caused by parasites that live and thrive in human red blood cells. The disease is not just a problem in developing countries but also in developed countries. As an infectious disease, one of malaria clinical symptoms is fever. Theoretically, it is stated that TNF-α belongs to the cytokines that can cause fever. This was a descriptive study. The body temperatures of patients with malaria fever were measured and the levels of serum TNF-α were examined by using the ELISA method. There were 40 samples obtained by using simple random sampling. The results showed that moderate level of TNF-α (100-500 pg/ml) was found in 36 samples (90%); low level of TNF-α (<100 pg/ml) in 4 samples (10%); meanwhile high level of TNF-α was not found (0%). The data showed that although in most samples the increased body temperatures were associated by the increased TNF-α levels, this condition was not found in all samples Conclusion: In general, the levels of TNF-α increased in patients with malaria fever.Keywords: TNF-α, malaria, fever  Abstrak: Malaria tergolong sebagai salah satu penyakit infeksi yang sangat berbahaya dan dapat menyebabkan kematian. Penyakit ini bukan hanya menjadi masalah di negara berkembang, namun juga di negara-negara maju. Malaria disebabkan oleh parasit yang hidup dan berkembang dalam sel darah merah manusia. Sebagai penyakit infeksi, malaria memiliki gejala-gejala klinis, salah satunya ialah demam. Dikatakan bahwa TNF-α merupakan sejenis sitokin yang menyebabkan terjadinya demam. Penelitian ini bersifat deskriptif dengan sampel sejumlah 40 penderita malaria yang diambil secara simple random sampling. Dalam penelitian ini dilakukan pengukuran suhu tubuh penderita malaria yang disertai demam dan kadar serum TNF-α dengan menggunakan metode ELISA. Hasil penelitian memperlihatkan bahwa kadar TNF-α sedang (100-500 pg/ml) ditemukan pada 36 sampel (90%), kadar rendah (<100 pg/ml) pada 4 sampel (10%), sedangkan kadar tinggi tidak ditemukan (0%). Data yag diperoleh memperlihatkan bahwa walaupun pada sebagian besar kasus peningkatan suhu tubuh disertai dengan peningkatan kadar serum TNF-α, namun kondisi ini tidak berlaku bagi seluruh sampel. Simpulan: Kadar TNF-α umumnya meningkat pada penderita malaria yang mengalami demam.Kata kunci: TNF-α, malaria, demam
PERBANDINGAN HASIL DETEKSI PLASMODIUM SPP ANTARA CARA PEMERIKSAAN MIKROSKOPIK TETESAN DARAH TEBAL DAN TEKNIK POLYMERASE CHAIN REACTION Sandra, Cindy; Tuda, Josef S. B.; Pijoh, Victor D.
Jurnal Biomedik : JBM Vol 6, No 1 (2014): JURNAL BIOMEDIK : JBM Maret 2014
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/jbm.6.1.2014.4161

Abstract

Abstract: Malaria is still a health problem in the world, especially in undeveloped countries. This disease is caused by protozoa of the genus Plasmodium and has two ways of transmission, naturally (Anopheles spp.) and unnaturally. WHO mentioned that in 2006 there were as many as 200-300 million cases of clinical malaria with 880,000 deaths. In 2012, it was recorded that there were a total of 8,691 malaria cases in North Sulawesi, Indonesia. Therefore, an early diagnostic tool with high sensitivity and specificity is needed. This study aimed to compare the sensitivity and specificity in detection of Plasmodium spp by using thick blood smear method to Polymerase Chain Reaction (PCR). This was a diagnostic test using blood samples of 30 malaria patients at Budi Mulia Hospital and Manembo-nembo Hospital Bitung from September 2013 - January 2014. Thick blood smears were prepared and microcopically tested, then the specimens were scrapped and be further tested by using the PCR. The microscopic test showed 20 positive samples meanwhile the PCR showed 24 positive samples. A diagnostic test using predictive table 2x2 indicated that the PCR had 100% sensitivity in general, 60% specifity, 83.3% positive predictive value, and 100% negative predictive value. Conclusion: Compared to the thick blood smear, the PCR was more accurate in detecting plasmodia in malaria cases with a moderate specificity value and a high sensitivity value.Keywords: thick blood smear, Polymerase Chain Reaction (PCR), sensitivity, specificity  Abstrak: Malaria merupakan salah satu penyakit yang masih menjadi masalah kesehatan di dunia terutama di negara-negara yang belum berkembang. Malaria disebabkan oleh protozoa dari genus Plasmodium dan ditularkan melalui dua cara yaitu alamiah melalui nyamuk Anopheles spp. dan tidak alamiah. WHO melaporkan bahwa pada 2006 terdapat 200-300 juta kasus malaria dengan 880.000 kematian. Di Provinsi Sulawesi Utara, Indonesia, dilaporkan total kasus malaria tahun 2012 sebesar 8.691. Oleh karena itu diperlukan suatu alat diagnostik dini dengan sensitivitas dan spesifisitas yang baik. Penelitian ini bertujuan untuk membandingkan tingkat sensitivitas dan spesifisitas hasil deteksi Plasmodium spp antara cara pemeriksaan mikroskopik tetesan darah tebal dan teknik Polymerase Chain Reaction (PCR). Penelitian ini merupakan uji diagnostik dengan sampel darah dari 30 pasien malaria di RS Budi Mulia dan RS Manembo-nembo Bitung sejak September 2013 - Januari 2014. Setelah disiapkan tetesan darah tebal, dilakukan pemeriksaan mikroskopik. Selanjutnya, spesimen darah dikerok dan diperiksa dengan PCR. Hasil pemeriksaan mikroskopik menunjukkan 20 sampel positif malaria sedangkan pemeriksaan PCR 24 sampel positif malaria. Tes uji diagnostik dengan tabel prediktif 2x2 mendapatkan tingkat sensitivitas PCR secara umum sebesar 100%, spesifisitas 60%, nilai duga positif 83,33%, dan nilai duga negatif 100%. Simpulan: Dibandingkan tetesan darah tebal, pemeriksaan PCR dapat mendeteksi secara lebih akurat adanya plasmodia pada kasus malaria, dengan nilai spesifitas sedang dan nilai sensitivitas tinggi.Kata kunci: tetesan darah tebal, Polymerase Chain Reaction (PCR), sensitivitas, spesifisitas
Infeksi Cacing Usus pada Penduduk Lanjut Usia di Desa Sawangan Kecamatan Airmadidi Kabupaten Minahasa Utara Lumbantobing, Greva R. I.; Tuda, Josef S. B.; Sorisi, Angle M. H.
Jurnal Biomedik : JBM Vol 12, No 1 (2020): JURNAL BIOMEDIK : JBM
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/jbm.12.1.2020.26933

Abstract

Abstract: Helminths are disease-causing agents which are very infectious and have very serious impacts and long-term effects but are still neglected. One of its risk factors is profession that has direct contact with soil, for examples farmers and planters. Sawangan village with a large area for farming is a home for many farmers. This study was aimed to obtain the prevalence of helminthic infection among the elderly population in Sawangan, Airmadidi District, North Minahasa Regency. This was a descriptive survey study. Respondents were elderly population of ≥60 years at Sawangan. Stool test resulted in five (5%) out of 100 samples were positive of helminthic infection. All positive samples contain hookworm. In conclusion, the prevalence of helminthic infection among elderly population at Sawangan, Airmadidi, North Minahasa Regency was 5%.Keywords: helminthic infection, elderly population. Abstrak: Cacing merupakan agen penyebab penyakit yang sangat infeksius serta memiliki dampak dan efek jangka panjang serius tetapi masih sering diabaikan. Salah satu faktor risiko ialah pekerjaan yang berkontak langsung dengan tanah, seperti petani dan pekebun. Desa Sawangan dengan lahan perkebunan yang luas memiliki banyak penduduk pekebun. Penelitian ini bertujuan untuk mengetahui prevalensi infeksi cacing pada penduduk lanjut usia yang berdomisili di Desa Sawangan Kecamatan Airmadidi Kabupaten Minahasa Utara. Jenis penelitian ialah survei deskriptif. Responden penelitian ialah penduduk lanjut usia ≥60 tahun di Desa Sawangan. Sampel yang diambil berupa feses. Pemeriksaan feses menunjukkan hasil 5 dari 100 (5%) sampel positif mengandung cacing. Seluruh sampel positif mengandung cacing tambang. Simpulan penelitian ini ialah prevalensi infeksi cacing pada penduduk lanjut usia di Desa Sawangan Kecamatan Airmadidi Kabupaten Minahasa Utara sebesar 5%.Kata kunci: infeksi cacing, penduduk lanjut usia.
Deteksi Antibodi Imunoglobulin M dan Imunoglobulin G Anti Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Abdul-Hamid, Nur A; Wahongan, Greta J. P.; Tuda, Josef S. B.
JURNAL BIOMEDIK : JBM Vol 13, No 1 (2021): JURNAL BIOMEDIK : JBM
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/jbm.13.1.2021.31753

Abstract

Abstract: COVID-19 is a disease caused by SARS-CoV-2 infection. Due to the increasing spread of COVID-19 infection in various countries, WHO declared the infection of COVID-19 as a pandemic situation. IgM and IgG antibodies test with the principle of immunochromatography is considered easier to perform than other test. The aims to detect anti SARS-CoV-2 IgM and IgG antibodies in North Sulawesi employees. This study is a descriptive study with cross sectional design. Antibody detection was carried out by dropping the serum of Balai Wilayah Sungai Sulawesi I's employees on the Clungene® COVID-19 IgG/IgM Rapid Test Cassette rapid diagnostic test based on antibody detected tool and interpreted based on the obtained result. As a result, tests which has been conducted on serum of 177 employees showed thirteen employees gave reactive results, where eight employees gave reactive IgM and IgG results, and five employees gave reactive IgG results. In conclusion, Immunoglobulin M and Immunoglobulin G were detected on some of Balai Wilayah Sungai Sulawesi I's employees.Keywords: COVID-19, SARS-CoV-2, IgM, IgG, immunochromatography  Abstrak: COVID-19 merupakan penyakit yang disebabkan oleh infeksi SARS-CoV-2. Oleh karena peningkatan penyebaran COVID-19 di berbagai negara, WHO mendeklarasikan infeksi COVID-19 sebagai situasi pandemi. Pemeriksaan antibodi IgM dan IgG dengan prinsip imunokromatografi dinilai lebih mudah dilakukan dibandingkan pemeriksaan lainnya. Penelitian ini bertujuan untuk mendeteksi antibodi IgM dan IgG anti SARS-CoV-2 pada karyawan kantoran Sulawesi Utara. Jenis penelitian yang digunakan adalah penelitian deskriptif dengan desain penelitian cross-sectional. Deteksi antibodi dilakukan dengan meneteskan serum karyawan Balai Wilayah Sungai Sulawesi I pada kit rapid diagnostic test berbasis deteksi antibodi Clungene® COVID-19 IgG/IgM Rapid Test Cassette dan diinterpretasikan sesuai hasil yang didapat. Hasil yang diperoleh dari pemeriksaan yang dilakukan pada 177 serum karyawan menunjukan tiga belas karyawan memberikan hasil pemeriksaan reaktif, dimana delapan karyawan dengan hasil IgM dan IgG reaktif, serta lima karyawan dengan hasil IgG reaktif. Sebagai simpulan, antibodi Imunoglobulin M dan Imunoglobulin G anti SARS-CoV-2 terdeteksi pada karyawan Balai Wilayah Sungai Sulawesi I.Kata Kunci: COVID-19, SARS-CoV-2, IgM, IgG, imunokromatografi
Co-Authors Abdul-Hamid, Nur A Agnes Kurniawan Agustina Tri Endharti Angle M. H. Sorisi Angle Sorisi Bagus Hermansyah Bakhtiar, Muh. Alvahrefi Bernadus, Janno Berty Bradly Burhan, Niniek C. Nagere, Andrew Cecilia S. Akay Cindy A. Batti Cindy Sandra D. Pijoh, Victor Didi Candradikusuma Diptyanusa, Ajib Erma Sulistyaningsih Finka Tangel, Finka Firra, Andi H. F. Greta J. P. Wahongan Greta Wahongan Herman Warouw Hidayat Sujuti Hiroyuki Matsuoka Ivan T. Sigarlaki, Ivan T. J. Kepel, Billy J. Runtuwene Jeanette I. Ch. Manoppo Jein Olifia Kapantow Kristanto, Erwin Gidion Kristin R. Walangare Labaiga, Natasya G.E. Lampus, Harsali Fransiscus Loeki Enggar Fitri Luis, Renjer Lumbantobing, Greva R. I. M. H. Sorisi, Angle Martha Marie Kaseke Megaria Rompas Meilany Tangkilisan Melisa Pantow Mengko, Stevano Mesaulawan, Septyarini Monica Thiosanto Muhammad Fikri Nakatsu, Masami Numansyah, Muhamad Patris J. Lombogia Priscilia, Lumataw Fransisca Rading, Fabio B. Ram Marnex Tampilang Regina C. Tawas Rianna J Sumampouw Rina Kundre Samsul Arifin Sartika, Sitha Senduk, Evy Beatrix Seroan, Alfa Yoan Simbala, Marsyella Arystia Sorisi, Angle Maria Hasthee Sorisi, Angle Maria Hesthee Stefany P. Kristin, Stefany P. Stella E. Lumiu Sumual, Rama L. Tati Ponidjan Ticoalu, Eliza Rolanda Trovancia, Grace Umar Zein Umbas, Irene Megawati Van Basten, Juan Victor D. Pijoh Wahongan, Greta J.P. Wahongan, Greta Jane Pauline Wijaya, Raditya Putri Yohanes C. W. Kawulur