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All Journal Tropical Medicine Journal Journal of the Medical Sciences (Berkala Ilmu Kedokteran) BERKALA SAINSTEK Jurnal Kesehatan Masyarakat Indonesian Journal of Tropical and Infectious Disease Majalah Obat Tradisional Unnes Journal of Public Health JFM (Jurnal Farmasi Malahayati) Cermin Dunia Kedokteran JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Berita Kedokteran Masyarakat Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Indonesian Journal of Pharmacology and Therapy Berita Biologi

Sitti Rahmah Umniyati

Fakultas Kedokteran Universitas Gadjah Mada

Author-ID : 407935

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health

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Comparison of Immunochromatography Method and Immunocytochemistry Method in Rapid Detection of NS-1 Antigen in Dengue Infection How Tien Jack; Sitti Rahmah Umniyati; Elsa Herdiana Murhandarwati
Tropical Medicine Journal Vol 3, No 1 (2013): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

ABSTRACT Introduction: Rapid test kit based on immunochromatography test (ICT) in detecting dengue NS-1 antigen for early dengue infection is available in the market. Its availability allows earlier management for dengue infected patient but it remains costly to most people. Recently, Dengue Team of Universitas Gadjah Mada has developed monoclonal antibodies to detect the presence of dengue NS-1 antigen in leucocytes of infected patients based on Streptavid in Biotin Peroxidase Complex (SBPC) immunocytochemistry method.Objectives: The objective of this study is to determine the validity of the immunochromatography (SD Dengue NS1 Ag) method by determining kappa agreement index between two observers, and to compare the diagnostic performances of ICT and immunocytochemistry methods in detecting dengue NS1 antigen in the blood samples.Methods: A cross sectional study design is used. This study uses 35 blood plasma remains from a previous study conducted on RT-PCR method. Three drops of blood plasma were added into the well of SD Dengue Duo NS1 and results were read after 15-20 minutes. The diagnostic performances of ICT which defined by sensitivity, specificity, positive predictive value and negative predictive value were calculated and compared to secondary data of immunocytochemistry result from the same blood samples, with reference of RT-PCR as a gold standard. A McNemar’s test was conducted and p value less than 0.05 was considered as significant different.Result: Detection of dengue infection by using SD Dengue NS1 Ag has strong agreements between two observers with kappa value of 1, and the sensitivity of 50%, specificity of 91%, positive predictive value of 92% and negative predictive value of 45% with reference of RT-PCR as a gold standard. Meanwhile sensitivity and specificity value of the immunocytochemistry test were 88% and 100% respectively, and the positive and negative predictive values were 100.0% and 70.0% respectively with reference of RT-PCR as a gold standard. The immunocytochemistry assay showed overall accuracy of 91,0%.Conclusion: Immunochromatography (SD Dengue NS1 Ag) method to detect NS-1 antigen has less sensitivity and specificity compared to SBPC immunocytochemistry method.Keyword: Immunocytochemistry, Immunochromatography, Streptavidin Biotin Peroxidase Complex (SBPC), NS-1 Ag, dengue

The Effects of Curcumin Against Dengue-2 Virus Based on Immunocytochemistry Technique Dewi Marbawati; Sitti Rahmah Umniyati
Tropical Medicine Journal Vol 3, No 2 (2013): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

ABSTRACT Introduction: Dengue is the most important mosquito-borne flavivirus disease. The number of Dengue cases in Indonesia in 2010 range from 150,000 cases with the deaths of around 1,317 people. Huge number of cases have made Indonesia was the first ranked as the state with the highest Dengue cases in the ASEAN region and the world's second ranking after Brazil. The drugs or antibiotics that can be administered effectively to cure this disease has not been found yet. Many study have been done and some that have been reported include viral RNA synthesis inhibitors, protein inhibitors of NS3 helicase and protease and inhibitors that inhibit Dengue virus maturation. Curcumin have preventive activity against several viruses: vasicular stomatis ( VSV ), HSV 1 and 2, parainfluenza - 3, reovirus - 1, feline corona virus, feline herpes virus . Curcumin also known have ubiquitin proteasome inhibition system was able to decrease the production of Japanese ensefalitis virus.Objectives: This study aims to determine safe concentrations of curcumin against vero cells (cytotoxic test results) and know the Dengue-2 antiviral potency of curcumin.Methods: Including quasi-experimental study. The anti viral potency of curcumin seen from the result of immunocytochemistry Streptavidin Biotin Peroxidase Complex (SBPC). Data were analyzed by ANOVA.Results: The results showed that secure concentrations from cytotoxic of curcumin against vero cells is 6.25 ppm. The calculation of positive rate from immunocytochemistry in vero cells infected by Dengue - 2 incubation 1 and 3 days were the result is significantly different than the control.Conclusion: The secure concentration of curcumin against vero cells was 6.25 ppm and curcumin was able to lower the positive rate due to Dengue-2 infection.Key Words : Dengue virus, Curcumin, Immunocytochemistry.

The Effect of Anticoagulant in Blood Meal Source on the Aedes aegypti Reproductive Ability in Laboratory Novyan Lusiyana; Budi Mulyaningsih; Sitti Rahmah Umniyati
Tropical Medicine Journal Vol 3, No 2 (2013): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

ABSTRACTIntroduction: Aedes aegypti is one of the major vectors of dengue hemorrhagic fever (DHF) that can be reared in laboratory. Artificial membrane feeding (AMF) assay is used as a simulated host to blood feeding mosquitoes in laboratory.Objectives: The purpose of this study are to investigate the effect of heater and the most widely used anticoagulant of K3EDTA, heparin and sodium citrate on blood feeding sucsses, feeding rate, fecundity, hatchability, preadult development and survival rate of Aedes Aegypti colonies maintained by AMF system compared to direct human feeding.Methods: The system consisted of AMF with parafilm membrane which are warmed and not warmed by a waterbath. Human blood samples were used to feed Aedes aegypti using AMF. The number of eggs were counted seven days after feeding after mosquitoes laid the eggs. Every eggs were hatched in a 500 mL of rearing glass to evaluate the hatchability and preadult development. Survival rate is evaluated after blood feeding until 30 days. Data was analyzed using one-way ANOVA and paired t-tests and a p value <0.05 considered as significantly difference.Results: Blood feeding success of Aedes aegypti was not significantly differ when offered blood meal using anticoagulant heparin with heater (82.22%) compare to that of control groups (81.67%) (p=0.917). There was a significant difference in feeding rate (p=0.000), fecundity (p=0.000), hatchability (p=0.000) between groups. All results were higher in heparin than K3EDTA and sodium citrate, but in pre adult development and survival rate K3EDTA showed better result than that of control, heparin and sodium citrate groups. So this anticoagulant was acceptable for maintenance of laboratory colonies of Aedes aegypti.Conclusion: We conclude that heater can increase the blood feeding sucsses. The K3EDTA, heparin and sodium citrate can affect the feeding rate, fecundity, hatchability, and preadult development, but do not affect survival rate. Heparin can be used for routine colonization of laboratory strain of Aedes aegypti with AMF assay.Keywords: Anticoagulants, artificial membrane feeding, reproductive ability, survival rate

Co-Authors -, Juffrie Adi Heru Sutomo Aditya Lia Ramadona Ajib Diptyanusa Amin, Siti Asiyah Fitria Apriyani Apriyani Ardhi, Khairil Bayu Satria Wiratama Budi Mulyaningsih Budi Mulyaningsih Budi Mulyaningsih Budi Mulyaningsih Dessy Triana Dewi Marbawati Dwi Aris Agung Nugrahaningsih Dwisiswanarum, Bella E. Elsa Herdiana Murhandarwati Eggi Arguni Ernaningsih Ery Agus Mardiansyah Farid Ayumi How Tien Jack Juffrie, Juffrie Lia Tri Hardiyanti Lucky Herawati Lutfan Lazuardi Mahardika Agus Wijayanti Marsetyawan HNE Soesatyo muhaimin saranani Muhamat, M Nababan, Resiany Nihayah, Husnatun Novyan Lusiyana Putri, Fitria Eka Qaimumanazalla, Hayu R.C. Hidayat Soesilohadi Reidinda Ratna Puspita Sari, Reidinda Ratna Puspita Resiany Nababan Rizki Wahistina Selvi Marcellia Siti Istianah Soroy Lardo Sri Mulyani Susi Iravati Susi Iravati SUWARNO HADISUSANTO Tri Baskoro Tunggul Satoto Yahiddin Selian Yuniyanti, Mia Munawaroh

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