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All Journal Jurnal Analis Medika Biosains (JAMBS) DIMAS: Jurnal Pengabdian Masyarakat Jurnal Ilmu Kesehatan Masyarakat Berkala Media Publikasi Promosi Kesehatan Indonesia (MPPKI) Kolaborasi Jurnal Pengabdian Masyarakat JOMPA ABDI: Jurnal Pengabdian Masyarakat Jurnal Pengabdian Kepada Masyarakat: Kesehatan (JPKMK) Journal of Medical Laboratory in Infectious and Degenerative Diseases Biomedika
Mufidah, Hartalina
Sekolah Tinggi Ilmu Kesehatan Wira Husada
Humanities Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Hubungan Pengetahuan, Sikap dan Perilaku Terhadap Motivasi Donor Darah Sukarela di PMI Kabupaten Sleman Yogyakarta : Association of Knowledge, Attitudes, and Behavior to Voluntary Blood Donation Motivation at PMI Sleman Regency Yogyakarta Mufidah, Hartalina; Handriani Kristanti; Eva Runi Khristiani
Media Publikasi Promosi Kesehatan Indonesia (MPPKI) Vol. 5 No. 5 (2022): May 2022
Publisher : Fakultas Kesehatan Masyarakat, Universitas Muhammadiyah Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (421.204 KB) | DOI: 10.56338/mppki.v5i5.2246

Abstract

Latar Belakang: Kebutuhan produk darah semakin meningkat seiring dengan peningkatan layanan transfusi darah. Ketersediaan produk darah sangat bergantung dari donor darah sukarela. Palang Merah Indonesia (PMI) Sleman merupakan salah satu Unit Donor Darah di Provinsi D.I. Yogyakarta yang belum mencapai kecukupan akan produk darah. Salah satu penyebabnya adalah baru 1% dari jumlah penduduk di Sleman yang menjadi pendonor darah sukarela. Rekrutmen donor yang tepat dengan mempertimbangkan faktor pengetahuan, sikap, dan perilaku merupakan strategi yang penting untuk memotivasi masyarakat melakukan donor darah secara sukarela. Tujuan: Penelitian ini bertujuan untuk menentukan faktor-faktor yang berhubungan dengan motivasi donor darah di PMI Kabupaten Sleman. Metode: Jenis penelitian ini adalah deskriptif analitik dengan desain studi cross-sectional yang dilaksanakan di PMI Sleman pada Januari-Desember 2021. Sampel penelitian adalah pendonor darah sukarela baru atau berulang yang lolos seleksi untuk donor darah di PMI Sleman dan menyetujui surat permohonan menjadi responden sebanyak 100 responden dan ditentukan menggunakan teknik accidental sampling. Analisis data penelitian dilakukan secara univariat dan bivariate. Hasil: Hasil penelitian menunjukkan bahwa terdapat signifikansi pada variabel perilaku pada nilai p-value=0,009<0,05. Kesimpulan: Terdapat hubungan perilaku dengan motivasi donor darah di PMI Sleman Yogyakarta.
Evaluation of Liver Function Through SGOT and SGPT Quantification in Rats Administered Polyethylene Terephthalate Microplastics Nadhifah, Diana; Mufidah, Hartalina; Imroatul Muflihah, Ahdiah
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 3 No. 1 (2025): Juni
Publisher : LPPM Universitas dr. Soebandi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36858/jmid.v3i1.29

Abstract

Background: Microplastics, as emerging environmental pollutants, can accumulate in target organs such as the liver and induce hepatotoxicity through oxidative stress mechanisms. Purpose: To determine the effect of PET microplastic exposure at various doses on liver transaminase enzyme levels (SGOT and SGPT) in white rats. Methods: This experimental study used 25 male white rats (Rattus norvegicus), aged 8–10 weeks, divided into five groups (n=5 per group): control (distilled water), P1 (0.4 mg/day), P2 (0.6 mg/day), P3 (0.8 mg/day), and P4 (1.0 mg/day) of orally administered PET microplastics for 28 days. SGOT and SGPT levels were measured using spectrophotometry. Statistical analysis was performed using ANOVA followed by Tukey-HSD or Bonferroni post hoc tests (α=0.05). Results: The SGPT level in the control group (61.48 ± 7.50 U/L) significantly increased to 114.40 ± 7.71 U/L (P1), 132.22 ± 18.41 U/L (P2), 191.78 ± 16.42 U/L (P3), and 227.26 ± 12.23 U/L (P4). The SGOT level in the control group (78.64 ± 9.48 U/L) significantly increased to 188.46 ± 2.55 U/L (P1), 195.10 ± 5.75 U/L (P2), 238.90 ± 41.34 U/L (P3), and 310.74 ± 26.66 U/L (P4). Statistical analysis revealed significant differences among groups (p < 0.05). The higher the PET microplastic dose, the higher the SGOT and SGPT levels observed. Conclusions: ET microplastic exposure induces hepatotoxicity, as evidenced by dose-dependent increases in SGOT and SGPT levels.
Antibacterial Activity of Papaya (Carica papaya L.) Seed Ethanol Extract against Staphylococcus aureus Isolated from Diabetic Ulcers Putri Aprilia, Diah; Mufidah, Hartalina; Wijaya, Anas Fadli
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 3 No. 1 (2025): Juni
Publisher : LPPM Universitas dr. Soebandi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36858/jmid.v3i1.30

Abstract

Background: Staphylococcus aureus is a bacterium that causes infections and is pathogenic to humans. Papaya (Carica papaya L.) seed extract contains various bioactive compounds, including tocopherols, terpenoids, flavonoids, alkaloids such as carpaine, and enzymes such as papain and lysozyme, which are known for their potential antimicrobial effects. Purpose: This study aimed to determine the antibacterial activity of papaya seed ethanol extract against S. aureus isolated from the foot ulcers of diabetic patients. Methods: An experimental quantitative study was conducted to evaluate the antibacterial activity of papaya seed ethanol extract at concentrations of 40%, 60%, and 80%. The extracts were prepared using the Ultrasonic-Assisted Extraction (UAE) method. Antibacterial activity was assessed by measuring the inhibition zones formed against S. aureus. Data were analyzed using a one-way ANOVA followed by a post hoc test to determine statistical significance. Results: The papaya seed extract exhibited concentration-dependent antibacterial activity against S. aureus. The mean inhibition zones were 0.00 mm at 40%, 15.07 mm at 60%, and 18.83 mm at 80%, whereas the positive control produced an inhibition zone of 46.36 mm. Conclusions: Papaya seed ethanol extract demonstrated antibacterial activity against S. aureus isolated from diabetic foot ulcers, with the strongest inhibition observed at the 80% concentration.
Effects of Various Mung Bean Decoction Doses on Hematological Profile in Anemic Wistar Rats Dwi Ayu Pratiwi, Nabila; Mufidah, Hartalina; Imroatul Muflihah, Ahdiah
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 3 No. 1 (2025): Juni
Publisher : LPPM Universitas dr. Soebandi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36858/jmid.v3i1.32

Abstract

Background: Anemia remains a significant global public health problem, with iron deficiency being the primary cause. Mung beans (Vigna radiata L.) are nutritionally rich plants containing iron and glycine, which are essential for hemoglobin synthesis and red blood cell formation Purpose: This study aimed to evaluate the effects of various doses of mung bean decoction on hematological profile improvement in anemic Wistar rats induced with sodium nitrite (NaNO₂). Methods: An experimental study was conducted from June to August 2024 involving 25 male Wistar rats divided into five groups: healthy control (aquadest), negative control (NaNO₂), and three treatment groups receiving NaNO₂ followed by mung bean decoction at doses of 0.2 ml, 0.4 ml, and 0.8 ml respectively. Blood samples were collected before and after treatment for hemoglobin level assessment. Data were analyzed using paired sample t-test and One-way ANOVA with post-hoc Tukey tests. Results: The One-way ANOVA test showed highly significant differences in hemoglobin level changes between groups (p < 0.0001). The 0.2 ml dose group exhibited the highest hemoglobin increase from 8.12 g/dl to 17.70 g/dl (p = 0.0002), followed by the 0.4 ml dose group with an increase from 7.32 g/dl to 16.08 g/dl (p < 0.0001), and the 0.8 ml dose group with an increase from 6.40 g/dl to 14.16 g/dl (p = 0.0005). Conclusions: Mung bean decoction effectively improved hemoglobin levels in anemic Wistar rats, with the 0.2 ml dose demonstrating the most pronounced therapeutic effect.
Identification of Candida albicans in the Urine of Pregnant and Non-Pregnant Women with UTI at X Hospital, Jember Pratiwi, Melia; Mufidah, Hartalina; Imroatul Muflihah, Ahdiah; Anggia Destiawan, Rian
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 3 No. 1 (2025): Juni
Publisher : LPPM Universitas dr. Soebandi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36858/jmid.v3i1.33

Abstract

Background: Urinary tract infections (UTIs) are a common condition in older women, but adolescents can also experience them. This condition can lead to other conditions, such as cancer and urinary tract stones. Candida albicans infection is one cause of UTIs, which is highly prevalent in both pregnant and non-pregnant women. This is due, in part, to hormonal influences. Purpose: This study aimed to knowing the difference in the growth of Candida albicans fungus in the urine of pregnant and non-pregnant women suffering from urinary tract infections. Methods: The study design was cross-sectional with 30 urine samples from pregnant women and 30 urine samples from non-pregnant women with urinary tract infections. The study was conducted from March to May 2025. The sampling technique used was purposive sampling. The data obtained were processed using SPSS chi-square test. If the chi-square test requirements were not met, decision-making was based on the fisher's exact test value. Results: Positive results for Candida albicans in the urine of UTI in pregnant women were 13 samples and 3 negative samples, while in non-pregnant women, 9 positive samples and 1 negative sample were obtained. UTI did not have a significant effect on the growth of Candida albicans because the statistical analysis of the chi-square test obtained a p-value of 0.370, where the p-value is greater than 0.05. Conclusions: There was no difference in the growth of Candida albicans fungus in the urine of pregnant and non-pregnant women with UTI.
Co-Authors Agustin, Ayu Tri AHDIAH IMROATUL MUFLIHAH Anas Fadli Wijaya Anggia Destiawan, Rian Anggitasari, Wima Dewi Nur Anggraeni dwi ayu pratiwi, nabila Elnaja, Dinar Anisa Eva Runi Khristiani, Eva Runi Handriani Kristanti Handriani Kristanti Hayati, Farhatul Hidayati, Sholihatil Humairoh, Riska Tsamarah Imroatul Muflihah, Ahdiah Kristanti, Handriani Marius Agung Sasmita Jati Nadhifah, Diana Pratiwi, Melia Purwanti, Ananda Kamila Nurcahya Putri Aprilia, Diah RIAN ANGGIA DESTIAWAN Shafrina Rohma, Hanna Sigit Purwanto sufi, Queen Nurul Susi Damayanti Trianggaluh Fauziah, Dina