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All Journal Indonesian Journal of Biotechnology Jurnal Media Tropika: Jurnal Pengabdian Kepada Masyarakat
Yulfia Nelymalik Selan, Yulfia Nelymalik
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Veterinary

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Histological Features of Catecholaminergic Neuron in Substantia Nigra Induced by Paraquat Dichloride (1,1-dimethyl-4,4 bipyridinium) in Wistar Rat as A Model of Parkinson Disease Pangestiningsih, Tri Wahyu; Wendo, Woro Danur; Selan, Yulfia Nelymalik; Amalo, Filphin Adolfin; Ndaong, Nemay Anggadewi; Lenda, Victor
Indonesian Journal of Biotechnology Vol 19, No 1 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (425.756 KB)

Abstract

Paraquat dichloride has been used by farmers as a herbicide to kill the grass. On the other hand, paraquatdichloride is harmful if enters to the body, causing Parkinson’s disease, since it is disrupting dopamineproduction in the substantia nigra pars compacta or dopamine pathways Nigro striatal pathway. The studywas done to fi nd out the histological changes of catecholaminergic neurons and Nigro striatal pathway causedby paraquat dichloride treatment in Wistar rats as a model of Parkinson’s disease.Twenty-two Wistar rats 3,5 months old were divided into 4 groups, 5 rats each. Group I (control group)were injected with aquabidest, while groups II, III, and IV were injected intraperitoneally with paraquatdichloride in aquabidest, with the dosage 5 , 10 and 15 mg/kg bw respectively. The rats were injected onceper week for 6 weeks. Three days after the last injection, the rats were anesthetized using xylasin (2 mg/kg)and ketamine (20 mg/kg) intramuscularly, and then were intracardiac perfused using physiological saline asprerinse solution, followed by 10% buffered formalin solution as a fi xative. After animals were fi xed, the brainswere removed and embedded in paraffi n block and cut in 12 μm thickness for immunohistochemistry stainingusing tyrosine hydroxylase antibody. The results of staining then were observed under light microscope andanalyzed descriptively.The results showed that the catecholaminergic neurons were distributed in the substantia nigrapars compacta in all treatment groups, however, the cell density were found decreased only in group IV.Catecholaminergic neurons appear in the bipolar and multipolar form, while dopamine ‘Nigro striatal pathway’was found exist in all treatment groups. From our study, histologycally the decreased of catecholaminergicneurons is only found in rats that received paraquat dichloride in dose 15 mg/kg bw for 6 weeks.
PEMBERDAYAAN KELOMPOK TANI TERNAK FATUKOA I DI KELURAHAN FATUKOA DENGAN PEMANFAATAN FERMENTASI BATANG POHON PISANG SEBAGAI PAKAN HEWAN Tangkonda, Elisabet; Maha, Inggrid Trinidad; Gaina, Cynthia Dewi; Deta, Herlina Umbu; Wea, Redempta; Ngasi, Bernadete Dwiyuni; Puspita, Anggi; Joesoef, Jayusman Arsiyanti; Selan, Yulfia Nelymalik; Widi, Antin Yeftanti Anggraeni; Gelolodo, Maria Aega
Jurnal Media Tropika Vol 4 No 1 (2024): Jurnal Media Tropika
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/mediatropika.v4i1.17075

Abstract

SOSIALISASI DAN EDUKASI RABIES DALAM PROGRAM KKN DI DESA CAMPLONG II KECAMATAN FATULEU KABUPATEN KUPANG Nitbani, Heny; Selan, Yulfia Nelymalik; Saputra, Agus; Ndaong, Nemay Anggadewi; Laut, Meity Marviana; Maha, Inggrid Trinidad
Jurnal Media Tropika Vol 5 No 1 (2025): Jurnal Media Tropika
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/mediatropika.v5i1.21249

Abstract

Rabies is a fatal zoonotic disease and is still a threat to public health, especially in areas with high populations of disease-carrying animals. In 2024, there was a case of rabies in Sillu village, one of the villages in Kupang Regency. This village area is adjacent to Camplong II village, which is the locus of the Undana Veterinary Study Program KKN. Camplong II village is a strategic location for implementing rabies socialization and education programs. This activity aimed to increase public knowledge and awareness, especially among school children, regarding the dangers of rabies, prevention, and initial handling of bites from rabies-transmitting animals (HPR). The methods used were pre-test, socialization and education, distribution of pamphlets, interactive discussions, and post-tests. The evaluation results showed an 82% increase in children's understanding of rabies after the activity took place. This program shows that the right socialization and education approach can increase public awareness in efforts to prevent rabies.
MEWARNAI SAMBIL BELAJAR: UPAYA EDUKASI RABIES PADA ANAK SEKOLAH DASAR DI DESA NUNHILA, TIMOR TENGAH SELATAN Ndaong, Nemay Anggadewi; Laut, Meity Marviana; Nitbani, Heny; Amalo, Filphin Adolphin; Selan, Yulfia Nelymalik; Maha, Inggrid Trinidad; Saputra, Agus
Jurnal Media Tropika Vol 5 No 1 (2025): Jurnal Media Tropika
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/mediatropika.v5i1.21482

Abstract

Rabies remains a critical public health concern, particularly in areas with limited resources and insufficient preventive efforts. Early education plays a vital role in raising awareness and promoting preventive behavior. This community service activity aimed to educate elementary school children about rabies through a creative and enjoyable method—coloring activities. The program was conducted at SD Inpres Nenonaheun, located in Nunhila Village, Mollo Barat Sub-district, Timor Tengah Selatan Regency, East Nusa Tenggara, Indonesia. It involved interactive coloring sessions using rabies-themed educational materials specifically designed for children aged 7 to 12. This activity combined visual learning and storytelling to convey essential information about rabies transmission, symptoms, and prevention. Evaluation through pre- and post-activity questionnaires revealed a significant improvement in the children's understanding of rabies. The use of coloring activities as an educational tool proved to be an effective strategy for raising awareness among elementary school students, indicating its potential for broader application in similar rural settings.
Co-Authors Amalo, Filphin Adolphin Deta, Herlina Umbu Filphin Adolfin Amalo Gaina, Cynthia Dewi Heni Pujiastuti Joesoef, Jayusman Arsiyanti Maha, Inggrid Trinidad Maria Aega Gelolodo Meity Marviana Laut Nemay Anggadewi Ndaong, Nemay Anggadewi Ngasi, Bernadete Dwiyuni Nitbani, Heny Puspita, Anggi Redempta Wea Saputra, Agus Tangkonda, Elisabet Tri Wahyu Pangestiningsih Widi, Antin Yeftanti Anggraeni Woro Danur Wendo