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All Journal Indonesian Journal of Biotechnology JURNAL KAJIAN VETERINER Jurnal Media Tropika: Jurnal Pengabdian Kepada Masyarakat Jurnal Veteriner Nusantara Jurnal Ilmu-Ilmu Pertanian Buletin Veteriner Udayana
Nemay Anggadewi Ndaong
Department Of Anatomy, Physiology, Pharmacology And Biochemistry, Faculty Of Medicine And Veterinary Medicine, Nusa Cendana University, Kupang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Veterinary

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Histological Features of Catecholaminergic Neuron in Substantia Nigra Induced by Paraquat Dichloride (1,1-dimethyl-4,4 bipyridinium) in Wistar Rat as A Model of Parkinson Disease Pangestiningsih, Tri Wahyu; Wendo, Woro Danur; Selan, Yulfia Nelymalik; Amalo, Filphin Adolfin; Ndaong, Nemay Anggadewi; Lenda, Victor
Indonesian Journal of Biotechnology Vol 19, No 1 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (425.756 KB)

Abstract

Paraquat dichloride has been used by farmers as a herbicide to kill the grass. On the other hand, paraquatdichloride is harmful if enters to the body, causing Parkinson’s disease, since it is disrupting dopamineproduction in the substantia nigra pars compacta or dopamine pathways Nigro striatal pathway. The studywas done to fi nd out the histological changes of catecholaminergic neurons and Nigro striatal pathway causedby paraquat dichloride treatment in Wistar rats as a model of Parkinson’s disease.Twenty-two Wistar rats 3,5 months old were divided into 4 groups, 5 rats each. Group I (control group)were injected with aquabidest, while groups II, III, and IV were injected intraperitoneally with paraquatdichloride in aquabidest, with the dosage 5 , 10 and 15 mg/kg bw respectively. The rats were injected onceper week for 6 weeks. Three days after the last injection, the rats were anesthetized using xylasin (2 mg/kg)and ketamine (20 mg/kg) intramuscularly, and then were intracardiac perfused using physiological saline asprerinse solution, followed by 10% buffered formalin solution as a fi xative. After animals were fi xed, the brainswere removed and embedded in paraffi n block and cut in 12 μm thickness for immunohistochemistry stainingusing tyrosine hydroxylase antibody. The results of staining then were observed under light microscope andanalyzed descriptively.The results showed that the catecholaminergic neurons were distributed in the substantia nigrapars compacta in all treatment groups, however, the cell density were found decreased only in group IV.Catecholaminergic neurons appear in the bipolar and multipolar form, while dopamine ‘Nigro striatal pathway’was found exist in all treatment groups. From our study, histologycally the decreased of catecholaminergicneurons is only found in rats that received paraquat dichloride in dose 15 mg/kg bw for 6 weeks.
Histological Features of Catecholaminergic Neuron in Substantia Nigra Induced by Paraquat Dichloride (1,1-dimethyl-4,4 bipyridinium) in Wistar Rat as A Model of Parkinson Disease Tri Wahyu Pangestiningsih; Woro Danur Wendo; Yulfia Nelymalik Selan; Filphin Adolfin Amalo; Nemay Anggadewi Ndaong; Victor Lenda
Indonesian Journal of Biotechnology Vol 19, No 1 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (425.756 KB) | DOI: 10.22146/ijbiotech.8638

Abstract

Paraquat dichloride has been used by farmers as a herbicide to kill the grass. On the other hand, paraquatdichloride is harmful if enters to the body, causing Parkinson’s disease, since it is disrupting dopamineproduction in the substantia nigra pars compacta or dopamine pathways Nigro striatal pathway. The studywas done to fi nd out the histological changes of catecholaminergic neurons and Nigro striatal pathway causedby paraquat dichloride treatment in Wistar rats as a model of Parkinson’s disease.Twenty-two Wistar rats 3,5 months old were divided into 4 groups, 5 rats each. Group I (control group)were injected with aquabidest, while groups II, III, and IV were injected intraperitoneally with paraquatdichloride in aquabidest, with the dosage 5 , 10 and 15 mg/kg bw respectively. The rats were injected onceper week for 6 weeks. Three days after the last injection, the rats were anesthetized using xylasin (2 mg/kg)and ketamine (20 mg/kg) intramuscularly, and then were intracardiac perfused using physiological saline asprerinse solution, followed by 10% buffered formalin solution as a fi xative. After animals were fi xed, the brainswere removed and embedded in paraffi n block and cut in 12 μm thickness for immunohistochemistry stainingusing tyrosine hydroxylase antibody. The results of staining then were observed under light microscope andanalyzed descriptively.The results showed that the catecholaminergic neurons were distributed in the substantia nigrapars compacta in all treatment groups, however, the cell density were found decreased only in group IV.Catecholaminergic neurons appear in the bipolar and multipolar form, while dopamine ‘Nigro striatal pathway’was found exist in all treatment groups. From our study, histologycally the decreased of catecholaminergicneurons is only found in rats that received paraquat dichloride in dose 15 mg/kg bw for 6 weeks.
Efek Pemaparan Deltamethrin pada Broiler Terhadap Aktivitas Enzim Alanin Aminotransferase, Aspartat Aminotransferase dan Gambaran Histopatologi Hepar Nemay Anggadewi Ndaong; Agustina Dwi Wijayanti; Sitarina Widyarini
JURNAL KAJIAN VETERINER Vol 2 No 1 (2014): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v2i1.990

Abstract

The purpose of this study is to determine the effects of deltamethrin exposure on the Broiler’s liver histopathological feature, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) enzyme activity. Fourty DOC Broilers strain New Loghman are divided into four group of ten and they were adapted for 5 days prior to the treatment. Group I (KI) is a control group, group II were given 20 mg/L deltamethrin, group III were given 10 mg/L deltamethrin and exposure deltamethrin concentration 10 mg/L and group IV were given 5 mg/L delthametrin. Deltamethrin was mixed with drinking water and then was given to the treatment group for 30 days. Blood samples were taken on day 0, day 15 and day 30 of treatment to determine of ALT and AST enzyme activity. On day 35, all animal were sacrificed, liver were taken out and fixed in 10% of buffer formalin for microscopic examination. Results of the AST enzyme activity shows that exposure to 20 mg/L and 10 mg/L deltamethrin for 30 days resulted in the histopathological changes of the liver, such as, fatty degeneration, necrosis on liver cells, inflamation of the liver, and necrosis on liver cells without the infiltration of inflamation cells. It is conclude that exposure to 20 mg/L deltamethrin for 30 days resulted in an increase in AST enzyme activity which is supported liver histopathological changes: fatty degeneration, necrosis, inflamation, and necrosis on liver cells without the infiltration of inflamation cells.
Identifikasi Boraks, Formalin dan Kandungan Gizi serta Nilai Tipe pada Bakso yang Dijual di Lingkungan Perguruan Tinggi di Kota Kupang Thedyastry Pandie; Diana A. Wuri; Nemay Anggadewi Ndaong
JURNAL KAJIAN VETERINER Vol 2 No 2 (2014): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v2i2.1003

Abstract

Penelitian ini bertujuan untuk mengidentifikasi penambahan boraks, formalin dan uji kandungan gizi serta TPC pada bakso daging sapi yang dijual di Lingkungan perguruan tinggi di Kota Kupang. Boraks dan formalin merupakan agen anti bakteri yang dapat menghambat pertumbuhan bakteri, dan apabila dicampurkan kedalam makanan dapat berbahaya bagi kesehatan karena boraks dan formalin bersifat karsinogenik. Lokasi pengambilan sampel dilakukan di kantin Universitas di Kota Kupang yaitu kantin Undana (2), kantin Unwira (3), kantin Politani (2) dan kantin PGRI (1), total sampel pengujian yaitu 8 sampel. Pengujian boraks dan formalin dilakukan pada laboratorium FKH Undana, dan diidentifikasikan menggunakan kit Easy Test®, pengujian kandungan gizi total protein kasar dan lemak kasar dilakukan di laboratorium Kimia dan Pakan Fakultas Peternakan Undana, serta pengujian TPC dilakukan pada laboratorium Bioreproduksi dan Kesehatan Ternak Fakultas Peternakan Undana. Hasil penelitian menunjukkan negatif untuk identifikasi boraks dan formalin. Berdasarkan SNI 01-3818-1995, standar yang ditetapkan untuk kandungan protein adalah minimal 9,0%, dan lemak maksimal 2,0%. Hasil pengujian protein menunjukkan 3 dari 8 sampel atau 37,5% sampel yang tidak memenuhi standar SNI. Hasil pengujian lemak terdapat 5 dari 8 sampel atau 62,5% sampel yang melebihi batas maksimum SNI. Berdasarkan SNI 01-3818-1995 batas cemaran mikroba pada bakso adalah 1x10³, dan pada hasil penelitian ini didapatkan bahwa terdapat 3 sampel dari 8 sampel atau sebanyak 37,5% sampel yang melebihi batas cemaran maksimum. Dari data diatas dapat disimpulkan, 2 dari 8 sampel atau 25% dari total sampel bakso daging sapi yang dijual di Lingkungan perguruang Tinggi di Kota Kupang aman dari bahaya boraks, formalin, dan kandungan gizi serta bahaya mikroorganisme.
Pengaruh Pemberian Ekstrak Etanol Daun Sambiloto( Andrographis paniculata Nees) Terhadap Histopatologi Pankreas Mencit ( Mus musculus ) Diabetes Melitus (DM) Tipe I Dwielma C. Nubatonis; Nemay Anggadewi Ndaong; Yulfia Nelymalik Selan
JURNAL KAJIAN VETERINER Vol 3 No 1 (2015): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v3i1.1028

Abstract

Diabetes mellitus (DM) is a metabolic disorder which damage the beta cells (β) which led to the destruction of langerhans of pancreas gland. Diabetes mellitus is characterized by increased blood glucose levels and progressive changes to the structure of the histopathology of the pancreas. The purpose of this study was to determine the effect of ethanol extract of sambiloto leaf ( Andrographis paniculata Nees) on pancreatic histopathology of Alloxan induced diabetic mice. This research is an experimental research design by Post Test Randomized Control Group Design Experim ental animals used were mice ( Mus musculus) 12 sample of mice ±2 weeks old were adapted for 5 days. Mice were divided into 4 groups, namely, negative control, positive contro l (3 days of alloxan injection) and diabetic mice treated with the ethanol extract of sambiloto leaf with 2 different doses (doses I =2,2mg/kg and dosesII=4,4mg/kg) for 14 days. On day15, mice were necropsy and pancreas were collected for further Hematoxiline Eosin (HE) coloration. The results showed that DM alloxan induced type 1 mice (Mus musclus), the pancreas characterized by the occurrence of necrosis, langerhans islet was not bounded clear and breakthe linkage of the langerhans islet with a cinar while on ethanol extract therapy of sambiloto leaf dose 2,2 mg/kg and 4,4 mg/kg in mice DM type I showed the results an improvement on the langerhans islet. Based on these results, it can be concluded that a dose of 2,2 mg/kg and 4,4 mg/kg is effective dose to repair the structure of langerhans islet of mice Type I DM, but it is more adv isable to us a therapeutic dose of 4,4 mg/kg because it changes the structure of Langerhans islet.
Identifikasi Kandungan Logam Berat Kadmium (Cd) Pada Sapi Potong Yang Dipelihara Di Tempat Pembuangan Akhir (Tpa) Kecamatan Alak, Kota Kupang Timothy Kondanamu Nangkiawa; Annytha Ina Rohi Detha; Nemay Anggadewi Ndaong
JURNAL KAJIAN VETERINER Vol 3 No 1 (2015): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v3i1.1030

Abstract

One of m ajor obstacle for cattle breeding enterprise in an urban area called Kupang city is the limited availability of grazing land . Attempting to overcome this , farmers in Kupang to utilize Alak final disposal (landfill) WITHOUT graz land of Alak. The purpose of this research was to identify the content of heavy metal cadmium (Cd) in cattle reared on waste/final disposal in Alak, Kupang . 12 blood samples were taken on the basis of various criteria , namely from the age group of 0 to 1.5 years of age; age group of 1.5 to 3 years and a group of more than 3 years. 3 ml blood samples were determined/ were measured by atomic absorption spectrophotometry in the Quality Testing Center and Animal Product Certification in Bogor. Results showed that the highest number was 0 .0297 ug/ml in the age group of 0 to 1.5 years) and the lowest was 0.0170 ug/ml in the age group over 3 years. Results showed that the highest number was 0.0297 ug/ml in the age group of 0 to 1.5 years) and the lowest was 0.0170 ug/ml in the age group ove r 3 years. In conclusion, 100% of heavy metal Cadmium was accumulated in beef cattle raised on Alak landfills and it might harmful to be consumed by people who live in kupang.
IN VITRO ANTHELMINTIC ACTIVITY OF Acalypha indica LEAVES EXTRACT AGAINST Haemonchus contortus Meity Marviana Laut; Nemay Anggadewi Ndaong; Yosephina Rengsina Delang
JURNAL KAJIAN VETERINER Vol 10 No 1 (2022): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v10i1.8899

Abstract

Haemonchosis adalah penyakit yang menyerang ruminansia kecil yang disebabkan oleh Haemonchus contortus. Pengendalian Haemonchosis dengan antelmintik sintetik belum berhasil karena berkembangnya resistensi H. contortus. Banyak penelitian telah dilakukan untuk mengetahui sifat antelmintik ekstrak tanaman sebagai alternative obat cacing modern. Penelitian ini bertujuan untuk mengevaluasi aktivitas antelmintik ekstrak etanol daun A. indica terhadap Haemonchus contortus dibandingkan albendazol 1% sebagai antelmintik standar. Uji motilitas dewasa dilakukan pada 75 cacing H. contortus dewasa pada ekstrak daun A. indica 0,5%, 1%, 2%. Setelah 6 jam paparan ekstrak, konsentrasi ekstrak yang lebih tinggi (2%) dapat mengurangi cacing dewasa (80%) dibandingkan dengan kontrol negatif dan ekstrak dengan konsentrasi lebih rendah. Dengan demikian, ekstrak etanol tanaman memiliki efek vermisidal yang menjanjikan terhadap H. contortus.
Perbandingan Onset, Durasi Anestesi Dan Masa Pemulihan Dari Pemberian Kombinasi Anestesi Acepromasin-Propofol-Ketamin Dan Midazolam-Propofol-Ketamin Pada Anjing Lokal Dalmasia Trisna Dhiu; Tri Utami; Nemay Anggadewi Ndaong
Jurnal Veteriner Nusantara Vol 4 No 1 (2021): Februari 2021
Publisher : Program Studi Kedokteran Hewan, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jvn.v4i1.6044

Abstract

This study aims to determine and compare the onset, duration and recovery period between the use of a combination of Acepromazine-Propofol-Ketamine and a combination of Midazolam-Propofol-Ketamine as an anesthetic drug in local dogs. Dogs are grouped into two treatment groups consisting of 3 local male dogs. Before the anesthetic agent was injected, each group was given premedication atropine sulfate at a dose of 0.02 mg / kg BW IM and 10 minutes later an induction of anesthesia was given. In treatment group 1, acepromazine (0.2 mg / kg BB) IV was administered, propofol (4 mg / kg BB) IV, and ketamine (4 mg / kg BW) IV. Group II was given anesthesia midazolam (0.2 mg / kg BB) IV, propofol (4 mg / kg BW) IV, and ketamine (4 mg / kg BW) IV. Observation and measurement of the onset, duration and recovery period were carried out after the animal was induced with an anesthetic agent. The results of the study obtained the following data: KI group produced an average onset of 94 seconds, duration of 30 minutes and recovery period of 30.33 minutes. KII produces an onset of 45.33 seconds, duration of 17.66 minutes and recovery time of 16 minutes. Based on the results of the study it can be concluded that the combination of midazolam-propofol-ketamine has a faster onset, duration and recovery period compared to the combination of acepromasizine-propofol-ketamine in local male dog.
EDUKASI MEMBUAT PAKAN SILASE KELOMPOK TANI TERNAK SANGGABETES, DESA HOLOAMA, KECAMATAN LOBALAIN, ROTE NDAO Nancy Foeh; Nemay Anggadewi Ndaong; Frans Umbu Datta; Annytha Ina Rohi Detha; Yohanes Timbun Raja Mangiut Ronael Simarmata; Putri Pandarangga
Jurnal Media Tropika Vol 3 No 1 (2023): Jurnal Media Tropika
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/mediatropika.v3i1.10297

Abstract

The use of forages such as odot grass in making silage during the dry season can be increased to improve the quality of animal feed. Anaerobic storage techniques can be carried out so that cooling can run perfectly. This training was carried out with the aim of equipping and increasing the understanding and abilities and skills of the Sanggabetes Livestock Farmers Group, Holoama Village, Lobalain District, Rote Ndao in increasing the nutritional value of forage feed. The stages of community service activities begin with socialization followed by hands-on training and end with monitoring and evaluation activities. The results of this service activity indicate that there was interaction and discussion between the resource person and the participants during the climb. The trainees and the Rote Ndao Animal Husbandry Service hope to continue the collaboration that has been established with mentoring activities for the Sanggabetes Livestock Farmer Group, Holoama Village, Lobalain District, Rote Ndao and other farmer groups in Rote Ndao in the utilization of forage and rice straw for cattle and other rumenance.
PENINGKATAN EKONOMI MASYARAKAT MELALUI BUDIDAYA AYAM KAMPUNG UNGGUL BALITBANGTAN (KUB) DI DUSUN BONEN BAUMATA, KECAMATAN TAEBENU Nitbani, Heny; Maha, Inggrid Trinidad; Amalo, Filphin Adolfin; Laut, Meity Marviana; Ndaong, Nemay Anggadewi
Jurnal Media Tropika Vol 3 No 2 (2023): Jurnal Media Tropika
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/mediatropika.v3i2.12657

Abstract

Community service activities are a form of actualization of the academic community's social responsibility and concern for the problems faced by the wider community. One form of this activity includes community assistance to expand knowledge and improve skills in order to improve living standards. This community service activity in Bonen Hamlet, Baumata Village, Taebenu District aims to provide assistance to PKK women regarding intensive and modern management of KUB chicken farms. The methods used in this service activity include building cages, counseling, handing over KUB chicken seeds, feed and cage equipment, as well as community assistance and ongoing monitoring. The conclusion obtained is an increase in community knowledge and skills regarding the advantages of raising KUB chickens, business opportunities for cultivating KUB chickens, management of the care and health of KUB chickens, as well as partner groups having sustainable entrepreneurial capital.
Co-Authors Agustina Dwi Wijayanti Amalo, Filphin Adolphin Andi Trisyono Annytha Ina Rohi Detha Bero, Catharina De Ricci Inye Bili, Agustinus Mulan Christvando Rendi Leba Tagu Bore Dalmasia Trisna Dhiu Deta, Herlina U. Diana A. Wuri Donny Satria, R. Gagak Dwielma C. Nubatonis Filphin Adolfin Amalo Foeh, Nancy Diana Ferderika Katerina Foeh, Nancy Diana Frederika Katerina Frans Umbu Datta Heni Pujiastuti heny Nitbani Inggrid Trinidad Maha Kallau, Novalino Harold Geoffrey Loe, Fhady Risckhy Maha, Inggrid Trinidad Mandala, Graziela Angelicha Meity Marviana Laut Nancy D. F. K. Foeh Nitbani, Heny Novian, Dede R. Novian, Dede Rival R. Gagak Donny Satria Roshelmi Talita Subu Taopan Saputra, Agus Sitarina Widyarini Thedyastry Pandie Timothy Kondanamu Nangkiawa Toha, Larry Richard Wellem Tri Utami Tri Wahyu Pangestiningsih Victor Lenda Woro Danur Wendo Y. Andi Trisyono Yohanes Timbun Raja Mangiut Ronael Simarmata Yosephina Rengsina Delang Yulfia N Selan Yulfia Nelymalik Selan Yulfia Nelymalik Selan, Yulfia Nelymalik Yustinus Oswin Primajuni Wuhan