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All Journal Jurnal Sain Veteriner Jurnal Veteriner Indonesian Journal of Biotechnology Jurnal Medik Veteriner
Woro Danur Wendo
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Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Veterinary

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Journal : Jurnal Veteriner

 1 
Identifikasi Keragaman Genetik Gen 12S Ribomsom RNA Sebagai Penanda Genetik untuk Penentuan Spesies Kuskus (IDENTIFICATION OF GENETIC DIVERSITY 12SRRNA GENES AS GENETIC MARKER FOR DETERMINING SPECIES CUSCUS) Rini Widayanti; Hery Wijayanto; Woro Danur Wendo; Rony Marsyal Kunda
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Cuscus is marsupial’s animal (Phalageridae) which has limited spread in eastern Indonesia (Sulawesi,Maluku, Papua), Australia and Papua New Guinea. The ex-situ and in-situ conservation of cuscus undercaptivating condition is an alternative solution to protect cuscus from extinction. This study aimed todetermine nucleotide sequence and genetic markers on 12Sr RNA gene with sequencing method of eachspecies on three islands. Whole genome DNA was extracted from 17 samples of cuscus obtained fromdifferent habitats, Sulawesi (2 individual), Maluku (7 individual), and Papua (8 individual) according tothe protocol of Qiamp DNA Blood Mini Kit (Qiagen), and then it was used as template for amplificationof 12Sr RNA gene by using PCR. PCR product were then purified using column chromatography and wereused as template for sequencing reaction. Result sequencing of 12Sr RNA gene were analyzed usingMEGA program version 6. PCR product gives a result nucleotida of 958 bp according to databasegenebank, sequencing product gives result nucleotida of 896 bp and found of 105 different nucleotide sites.Filogram based on nucleotide sequences 12SrRNA gene from Sulawesi cuscus is Ailurops ursinus whereasthe cuscus from Papua and Maluku is Phalanger sp. and Spilocuscus maculatus species. Thirteen nucleotidasites were found, sites no 67 (A/G), 89 (G/C), 137 (T/C), 285 (G/A), 468 (T/C), 595 (T/C, 598 (T/C), 647 (T/C),654 (G/A), 665 (T/C), 769 (C/T), 874 (C/T), and 876 (A/G) which can be used as genetic marker betweenPhalanger genera from Papua and Maluku, and three nucleotida sites (sites no 127 (G/A), 481 (C/T), and885 (T/C) can be used as genetic marker between Spilocuscus genera from Papua and Maluku.
Lumba-Lumba Hidung Botol Laut Jawa Adalah Tursiops aduncus Berdasar Sekuen Gen NADH Dehidrogenase Subunit 6 (VERIFICATION BOTTLENOSE DOLPHINS FROM JAVA SEA IS TURSIOPS ADUNCUS BASED ON GENE SEQUENCES OF NADH DEHYDROGENASE SUBUNIT 6) Rini Widayanti; Yuda Heru Fibrianto; Woro Danur Wendo
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Bottlenose dolphins (Tursiops sp.) is one of the aquatic mammals widely spread in the marines ofIndonesia archipelago, especially the Java Sea. The taxonomy of the genus Tursiops is still  controversial.The purpose of this study was to examine the molecular basis of Tursiops sp of Java sea marine origin onthe basis of its NADH dehydrogenase gene subunit 6 (ND6) sequences. Samples of blood were collectedfrom five male bottle nose dolphins from captivity of PT. Wersut Seguni Indonesia. DNA was isolated,amplified by polymerase chain reaction (PCR), sequenced, and analyzed the data using the MEGA v. 5.1program. The results of PCR amplification was 868 base pairs (bp), DNA sequencing showed that 528nucleotides were ND6 gene, nucleotide at the position of 387 could be used to distinguish the bottle nosedolphins Java marine origin with T. aduncus.   Filogram using Neighbor joining method based on thenucleotide sequence of the gene ND6, showed that bottle nose dolphins Java marine origin belong to groupof T. aduncus.
Co-Authors Arif Suhodo Bambang Dwi Haryanto Dwi Liliek Kusindarta Filphin Adolfin Amalo Hendri T.S. Saragih Heni Pujiastuti Hery Wijayanto Nemay Anggadewi Ndaong Nemay Anggadewi Ndaong, Nemay Anggadewi Rini Widayanti Rony Marsyal Kunda Siti Isrina Oktavia S. Tri Wahyu Pangestiningsih Victor Lenda Yuda Heru Fibrianto Yulfia Nelymalik Selan Yulfia Nelymalik Selan, Yulfia Nelymalik Yuliati Wahyu S.