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All Journal ACTA VETERINARIA INDONESIANA Jurnal Sain Veteriner Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences) Jurnal Kedokteran Hewan Jurnal Veteriner dan Biomedis Jurnal Ilmu-Ilmu Pertanian
Agustina Dwi Wijayanti
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Immunology & microbiology Medicine & Pharmacology Veterinary

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Isolasi dan identifikasi Escherichia coli dari Sumber Air Minum Kandang Broiler serta Uji Aktivitas Antibakteri Lidah Buaya Nuraini, Dian Meididewi; Andityas, Morsid; Paramarta, Adi; Najib, Nur Rohman; Wijayanti, Agustina Dwi
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol 10 No 2 (2020): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Vete
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/jipvet.v10i2.116

Abstract

Abstract Colibacillosis is one of the most problematic issues in the boiler industry. However, the antibiotic overuse has induced Escherichia coli resistance so that other alternative to reduce colibacillosis is needed. One of the alternatives is using aloe vera (Aloe barbadensis Miller), which has been widely used as an antibacterial agent. This study aims to isolate and identify E. coli from the broiler drinking water source and test the aloe vera antibacterial activity against it. Escherichia coli were isolated from well in three broiler farms in Moyudan District, Sleman, Yogyakarta that previously had colibacillosis. Escherichia coli were isolated using eosin methylene blue (EMB) agar and the metallic sheen colony was tested to confirm the biochemist reaction. The pure isolate of E. coli was used in the aloe vera inhibition test using Muller Hinton agar (MHA) by a Well Diffusion method. Aloe vera was processed using aquades and ethanol 70%. The aquades infusion was diluted into 12.5%, 25%, 50%, 75%, and 100% and the extract ethanol 70% was diluted into 10%, 12.5, %, 25%, 40%, and 50%. The bacterial identification showed that one of three samples contained E. coli which was then used for inhibition test. The result showed no inhibition zone in the aquades infusion while ethanol extract showed an inhibition zone in concentration 25%, 40%, and 50% of aloe vera extract with a diameter 19.5 mm, 24 mm, and 25 mm. It can be concluded that aloe vera ethanol extract has inhibitory activity against E. coli in poultry drinking water with a minimum concentration of 25%. Keywords: Aloe vera; Broiler drinking water; Escherichia coli; Inhibitory activity Abstrak Colibacilosis masih menjadi permasalahan dalam industri broiler. Penggunaan antibiotik berlebihan telah menyebabkan resistensi sehingga perlu alternatif lain. Salah satu alternatif adalah menggunakan bahan alami seperti adalah lidah buaya (Aloe barbadensis Miller) yang memilliki aktivitas antibakteri. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi E. coli dari sumber air minum di kandang broiler serta menguji aktivitas inhibisi lidah buaya terhadap bakteri tersebut. Air yang digunakan sebagai sumber E. coli berasal dari sumur di tiga peternakan broiler di Kecamatan Moyudan, Sleman, Yogyakarta yang memiliki riwayat infeksi colibacilosis. Bakteri E. coli diisolasi menggunakan media eosin methylene blue (EMB) dan diuji sifat biokimia untuk mengkonfirmasi sifat bakteri E. coli. Isolat murni E. coli digunakan pada uji daya hambat bakteri dengan metode difusi sumuran menggunakan media Muller Hinton Agar (MHA). Lidah buaya diproses menggunakan aquades dan ethanol 70%. Infusa aquades diencerkan menjadi konsentrasi 12,5%, 25%, 50%, 75%, dan 100% dan ekstrak ethanol 70% diencerkan menjadi 10%, 12., %, 25%, 40%, dan 50%. Hasil isolasi menunjukan bahwa satu sumber air dari sumur di Desa Kolowenang mengandung E. coli yang kemudian digunakan pada pengujian daya hambat. Hasil pengujian menunjukan tidak ada daya hambat yang terbentuk pada infusa aquades sedangkan ekstrak etanol lidah buaya 25%, 40%, dan 50% menunjukan adanya zona hambat sebesar 19,5 mm, 24 mm, dan 25 mm berturut-turut. Ekstrak etanol lidah buaya pada penelitian ini memiliki kemampuan menghambat pertumbuhan E. coli yang bersumber dari air minum broiler dengan konsentrasi terendah 25%. Kata kunci: Air minum broiler; Escherichia coli; Lidah buaya; Daya hambat bakteri
Efek Pemberian Antibiotik Kombinasi Spektinomisin dan Linkomisin Selama Satu dan Dua Minggu terhadap Hematologi Broiler Wibisono, Cahyo; Wijayanti, Agustina Dwi; Fadilla, Mirza Nur
Jurnal Veteriner dan Biomedis Vol. 2 No. 2 (2024): September
Publisher : Sekolah Kedokteran Hewan dan Biomedis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jvetbiomed.2.2.98-105.

Abstract

Pemberian antibiotik sebagai salah satu upaya terapi maupun pencegahan penyakit bakterial pada ayam tentu sangat penting mengingat broiler rentan terhadap penyakit. Salah satu antibiotik yang digunakan di Indonesia adalah kombinasi spektinomisin dan linkomisin. Status kesehatan ayam dapat diketahui melalui kondisi hematologinya. Tujuan penelitian ini untuk mengetahui kondisi hematologi berupa kadar hemoglobin, nilai hematokrit, total eritrosit, dan total leukosit pada broiler yang diberikan antibiotik kombinasi spektinomisin dan linkomisin selama satu dan dua minggu. Penelitian menggunakan 40 ekor Day Old Chicken (DOC) broiler yang terbagi dalam 3 perlakuan yaitu kelompok K (kontrol), kelompok A1 (pemberian antibiotik 1 minggu), dan kelompok A2 (pemberian antibiotik 2 minggu). Antibiotik kombinasi spektinomisin dan linkomisin diberikan secara oral lewat air minum dengan menggunakan dosis terapi sebesar 1,5 gram/ 2 liter mulai hari ke-18 sampai hari ke-24 untuk kelompok A1 dan untuk A2 dilanjutkan sampai hari ke-31. Pengambilan sampel darah dilakukan pada hari ke-32 untuk kelompok A1 dan hari ke-39 untuk kelompok A2 melalui vena brakialis. Sampel digunakan untuk mengukur kadar hemoglobin dengan spektrofotometer, nilai hematokrit dengan hematokrit scale reader, jumlah eritrosit dengan pipet thoma eritrosit “101”, jumlah leukosit dengan pipet eritrosit “11”. Data yang diperoleh dianalisis secara deskriptif dan statistik. Hasil penelitian menunjukkan kadar hemoglobin, nilai hematokrit, jumlah eritrosit, dan jumlah leukosit tidak ada perbedaan signifikan (p > 0,05). Kesimpulan dari pemberian antibiotik kombinasi spektinomisin dan linkomisin selama 1 dan 2 minggu tidak berpengaruh terhadap hematologi broiler.
Pengujian Efektivitas Levamisol terhadap Cacing Gastrointestinal pada Ayam Kampung asal Yogya-karta secara in Vitro Wijayanti, Agustina Dwi; Irawan, Aisha Pasha
Acta VETERINARIA Indonesiana Vol. 13 No. 2 (2025): Juli 2025
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.13.2.%p

Abstract

Levamisole is a broad-spectrum anthelmintic from the imidazothiazole derivative group for mammals and poultry. This study aims to evaluate the anthelmintic effectiveness of levamisole against gastrointestinal worms in domestic chickens in Yogyakarta. The findings of this study could potentially lead to the development of more effective strategies for controlling helminthiasis in poultry, thereby improving the health and productivity of domestic chickens. This study is an in vitro laboratory experiment that uses Ascaridia galli (AG) and Raillietina spp. (RL), in a petri dish with ten treatment groups (n=3). The groups consisted of two negative controls, 0.9% sodium chloride AG and RL (G1, G2), two groups of AG and RL in Carboxymethylcellulose Sodium (G3, G4), and levamisole treatment groups with doses of 0.02, 0.1, and 0.2% for three groups of AG (G5-G7) and three groups of RL (G8-G10), respectively. The worm’s mortality time was observed for 24 hours, while descriptive analyses were conducted between treatments with the scoring method. The 0.2% levamisole solution showed anthelmintic efficacy toward AG and RL of a domestic chicken, which in RL has the fastest time to die, 30 minutes of mortality time.
METODE ALTERNATIF ANALISIS INSEKTISIDA PERTANIAN DELTAMETHRIN MENGGUNAKAN KROMATOGRAFI CAIR KINERJA TINGGI PENGGUNAAN KOLOM C-18, FASE GERAK ASETONITRIL 80% DALAM AKUABIDES, LAJU ALIR FASE GERAK 1,6 ML/MENIT, DAN PANJANG GELOMBANG DETEKTOR UV-VIS 190 NM ( Donny Satria, R. Gagak; Sumiarto, Bambang; Trisyono, Andi; Wijayanti, Agustina Dwi
Jurnal Ilmu-Ilmu Pertanian Vol. 17 No. 1 (2013): Juli
Publisher : Politeknik Pembangunan Pertanian Yogyakarta-Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Deltamethrin is a common pesticide used in plants and animals. Research about this pesticide has been done with various methods. The purpose of this research was to validate the alternative test method of deltamethrin using High Performance Liquid Chromatography (HPLC). This research used pure deltamethrin. The tool in this research was HPLC Shimadzu 6.1 with 80% acetonitrile in aquabides for mobile phase, flow rate 1.6 ml/min and uv-vis detector wavelength 190 nm. The data were analyzed by calculated the average, standard deviation, and relative standard deviation. The results showed the average area for each concentration of 0.1 ug/ml, 0.25 ug/ml, 0.3 ug/ml, 0.5 ug/ml, 0.75 ug/ml, 1 ug/ml,2 ug/ml, and 5 ug/ml, were 52806.33, 119070.3, 137758.3, 220292, 339677.3, 449448.7, 856049.3, and 2,177,558, with a linear equation y = 43283x + 8455, R = 0,999. It could be concluded that the method is valid based on precision, acuration, specificity, limit of detection, limit of quantification, and linearity.
THE SEDATIVE STUDY OF TABAR KEDAYAN ROOT ETHANOLIC EXTRACT (Aristolochia foveolate) IN MICE (Mus musculus) Wijayanti, Agustina Dwi; Nugroho, Alvinia Rusandriani; Yanti, Desti Ika; Fitriana, Ida ida
Jurnal Kedokteran Hewan Vol 18, No 2 (2024): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v18i2.33759

Abstract

The study was conducted to know the sedation effect of Tabar kedayan (Aristolochia faveolate). Thirty male mice were grouped into six groups, and each group contained five mice which were given six treatments. Group I, II, III, and IV were treated orally with tabar kedayan root ethanolic extract (TKREE) with a single dose of 50, 100, 200, and 400 mg/kg body weight, group V was given chlorpromazine with a dose of 20 mg/kg, and group VI was given distilled water 10 mL/kg of body weight. Based on the analysis, the results of mice falling time for group I, II, III, IV, V, and VI were 238.93101.01, 208.38115.41, 240.69102.19, 276.4268.28, 31.6464.55, and 168.09126.82 seconds, respectively. The groups were significantly different from the positive control group (P0.05). Mice treated with TKREE fell longer than negative and positive controls. Based on these results, the administration of TKREE did not cause a sedation effect on mice; otherwise, may have potential as a stimulant, and studies are needed to provide scientific evidence to support this potential.
EVALUATION OF LAPAROTOMY SURGERY WOUND HEALING IN DOGS USING THERMOGRAPHIC ANALYSIS Wijayanti, Agustina Dwi; Purnomo, Agus; Wijayanto, Hery
Jurnal Kedokteran Hewan Vol 17, No 1 (2023): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v17i1.29785

Abstract

This study aimed to develop the use of thermographic analysis to evaluate wound healing for laparotomy surgery in animals. The experiment used nine adult dogs 5 males and 4 females, bodyweight 5-10 kg) that had undergone the same general anesthesia and laparotomy procedures. Evaluation of wound healing was performed at 24, 48, and 72 hours after surgery and 7 days after operations using a digital thermal camera. During each thermographic evaluation period, blood samples were taken for analysis of total leukocytes and leukocyte differential. The results of thermal imaging were compared with the values of inflammatory cells and the clinical condition of wound healing. Comparison of thermographic analysis with inflammatory status was evaluated using the regression equation and showed a strong correlation coefficient (Y= -0.4847 + 40.14, R2= 0.867), that is, the higher the temperature, the lower the inflammation. The conclusion of study is that thermographic analysis can be used to evaluate wound healing of laparotomy surgery in dogs.
EFEKTIVITAS ENROFLOKSASIN TERHADAP INFEKSI BAKTERI PADA SALURAN PENCERNAAN ULAR SANCA BATIK (Python reticulatus) Wijayanti, Agustina Dwi; Untari, Tri; Rosetyadewi, Antasiswa W.; Rahardjo, Slamet
Jurnal Kedokteran Hewan Vol 7, No 2 (2013): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v7i2.898

Abstract

Penelitian ini bertujuan mengetahui efektivitas enrofloksasin terhadap infeksi bakteri pada saluran pencernaan ular sanca batik (Python reticulatus). Ular yang digunakan berjumlah 10 ekor dan terindikasi klinis mengalami gangguan pencernaan berupa keradangan pada mulut. Sampel yang diambil adalah swab mulut dan kloaka untuk pemeriksaan mikrobiologi berupa isolasi dan identifikasi bakteri pada media brilliant green agar, Mc Conkay agar, triple sugar iron, dan pembiakan isolat murni. Setelah pengambilan sampel semua ular diinjeksi dengan enrofloksasin 5 mg/kg bobot badan, dosis tunggal secara intramuskular anterior. Pengamatan klinis dilakukan hingga semua ular dinyatakan sembuh dari keradangan mulut. Hasil pemeriksaan mikrobiologi menunjukkan adanya bakteri Salmonella sp., E. coli, dan Proteus sp. pada saluran pencernaan ular. Enrofloksasin yang diberikan secara injeksi intramuskular anterior mampu memberikan kesembuhan dalam rentang waktu 4-16 hari setelah pemberian.
Perilaku dan Gambaran Hematologis Beruk (Macaca nemestrina) yang Dipasang Bare metal stent pada Arteri Iliaca Arifianto, Dinar; Wijayanti, Agustina Dwi; Budhi, Setyo; Wijayanto, Heri; Siswomihardjo, Widowati
Jurnal Sain Veteriner Vol 43, No 2 (2025): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.70940

Abstract

Penyakit kardiovaskuler yang paling banyak diderita oleh masyarakat Indonesia adalah penyakit jantung coroner. Salah satu metode terapi jantung korner adalah dengan pemasangan stent jantung. Saat ini stent jantung yang digunakan oleh kardiolog di Indonesia masih mengandalkan produk impor. Saat ini Universitas Gadjah Mada (UGM) sedang mengembangkan stent dengan desain dan bahan yang telah diteliti sebelumnya yang disebut dengan INA-Stent. Tahap pre-klinis telah dilaksanakan dengan menggunakan hewan beruk (Macaca nemestrina). Pemasangan bare metal stent (tanpa salut obat) telah dipasang pada bulan September tahun 2018. Proses euthanasia dilaksanakan pada bulan September 2021 di ruang kateterisasi Rumah Sakit Hewan pendidikan Prof. Soeparwi Fakultas Kedokteran Hewan (FKH UGM). Sebelum pelaksanaan euthanasia diambil sampel darah sebanyak 1 ml pada tabung ethylene diamine tetra acetic acid (EDTA). Pemeriksaan darah menunjukkan total eritrosit, hemoglobin, PCV, MCV, total leukosit, dan trombosit dalam batas normal. Abnormalitas yang terjadi antara lain limfopenia, monositosis dan hipokromia.
Co-Authors Agus Purnomo Antasiswa W. Rosetyadewi Arifianto, Dinar Bambang Sumiarto Donny Satria, R. Gagak Fadilla, Mirza Nur Fitriana, Ida ida Heri Wijayanto Hery Wijayanto Irawan, Aisha Pasha Morsid Andityas Najib, Nur Rohman Nugroho, Alvinia Rusandriani Nuraini, Dian Meididewi Paramarta, Adi Setyo Budhi Slamet Rahardjo Tri Untari Wibisono, Cahyo Widowati Siswomihardjo Y. Andi Trisyono Yanti, Desti Ika