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All Journal ACTA VETERINARIA INDONESIANA Jurnal Sain Veteriner Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences) Jurnal Kedokteran Hewan Jurnal Veteriner dan Biomedis Jurnal Ilmu-Ilmu Pertanian
Agustina Dwi Wijayanti
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Immunology & microbiology Medicine & Pharmacology Veterinary

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Journal : Jurnal Ilmu-Ilmu Pertanian

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METODE ALTERNATIF ANALISIS INSEKTISIDA PERTANIAN DELTAMETHRIN MENGGUNAKAN KROMATOGRAFI CAIR KINERJA TINGGI PENGGUNAAN KOLOM C-18, FASE GERAK ASETONITRIL 80% DALAM AKUABIDES, LAJU ALIR FASE GERAK 1,6 ML/MENIT, DAN PANJANG GELOMBANG DETEKTOR UV-VIS 190 NM ( Donny Satria, R. Gagak; Sumiarto, Bambang; Trisyono, Andi; Wijayanti, Agustina Dwi
Jurnal Ilmu-Ilmu Pertanian Vol. 17 No. 1 (2013): Juli
Publisher : Politeknik Pembangunan Pertanian Yogyakarta-Magelang

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Abstract

Deltamethrin is a common pesticide used in plants and animals. Research about this pesticide has been done with various methods. The purpose of this research was to validate the alternative test method of deltamethrin using High Performance Liquid Chromatography (HPLC). This research used pure deltamethrin. The tool in this research was HPLC Shimadzu 6.1 with 80% acetonitrile in aquabides for mobile phase, flow rate 1.6 ml/min and uv-vis detector wavelength 190 nm. The data were analyzed by calculated the average, standard deviation, and relative standard deviation. The results showed the average area for each concentration of 0.1 ug/ml, 0.25 ug/ml, 0.3 ug/ml, 0.5 ug/ml, 0.75 ug/ml, 1 ug/ml,2 ug/ml, and 5 ug/ml, were 52806.33, 119070.3, 137758.3, 220292, 339677.3, 449448.7, 856049.3, and 2,177,558, with a linear equation y = 43283x + 8455, R = 0,999. It could be concluded that the method is valid based on precision, acuration, specificity, limit of detection, limit of quantification, and linearity.
Co-Authors Agus Purnomo Antasiswa W. Rosetyadewi Arifianto, Dinar Bambang Sumiarto Donny Satria, R. Gagak Fadilla, Mirza Nur Fitriana, Ida ida Heri Wijayanto Hery Wijayanto Irawan, Aisha Pasha Morsid Andityas Najib, Nur Rohman Nugroho, Alvinia Rusandriani Nuraini, Dian Meididewi Paramarta, Adi Setyo Budhi Slamet Rahardjo Tri Untari Wibisono, Cahyo Widowati Siswomihardjo Y. Andi Trisyono Yanti, Desti Ika