Diah Permata Wijayanti
Jurusan Ilmu Kelautan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Dipenogoro Jalan Prof. H. Soedarto, SH. Kampus Tembalang, Semarang 50275, Indonesia

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Kesuburan Perairan Berdasarkan Kandungan Nutrien pada Ekosistem Mangrove Desa Bedono, Demak Widiardja, Aufa Rifqi; Nuraini, Ria Azizah Tri; Wijayanti, Diah Permata
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v10i1.28480

Abstract

Nutrien memiliki peranan penting dalam pertumbuhan dan perkembangan biota laut dan merupakan hasil uraian dari bahan organik kompleks. Secara alamiah konsentrasi nutrien dalam perairan bervariasi dan dalam kondisi tertentu dapat terjadi keadaan di luar batas optimal bagi organisme. Penelitian ini bertujuan untuk mengetahui kandungan nutrien (Nitrat, Nitrit, Ammonium dan Phosphat) dan mengetahui tingkat kesuburan perairan Desa Bedono, Kecamatan Sayung, Kabupaten Demak sehingga dapat memberikan informasi dan acuan dalam memonitoring kandungan nutrient di perairan. Penelitian ini dilaksanakan pada bulan Januari 2020 di Perairan Desa Bedono, Kecamatan Sayung, Kabupaten Demak. Metode dalam penelitian ini adalah metode deskriptif sedangkan pemilihan lokasi pengambilan sampel menggunakan metode purposive sampling method. Pengambilan parameter lingkungan yang dibutuhkan dilakukan secara insitu dan sampel air di analisis di Laboratorium Teknik Lingkungan, Universitas Diponegoro. Pengambilan sampel dilakukan tiga kali pengulangan dengan menyimpan sampel air dengan botol sampel polyetilene yang dimasukan kedalam coolbox untuk menghindari masuknya sinar matahari. Hasil penelitian menunjukan nilai analisis kandungan nitrat pada perairan Desa Bedono, Kabupaten Demak berkisar antara 2,353 – 2,973 mg/L. Kandungan nitrit berkisar antara 0 – 0,01 mg/L, kandungan ammonium berkisar antara 14,815 – 18,239 mg/L, sedangkan kandungan phospat berkisar antara 0,04767 – 0,05133 mg/L. Berdasarkan kandungan nutrient Nitrat dan Phosphat kesuburan perairan berada dalam klasifikasi mesotrofik.Nutrient had an important role in growth and development of organism and are result of the decomposition from complex organic materials. Naturally, the concentration of nutrients in the water varies and under certain conditions it can occur outside the optimal limits that was declared safe for organism. This research aims to determine the level of water fertility based on the content of nitrate, nitrite, ammonium and phosphate in the Coastal Waters Of Bedono Village, Sayung District, Regency of Demak. This research was conducted on January 2020 in the Coastal Waters Of Bedono Village, Sayung District, Regency of Demak. The method used in this research is descriptive method while the selection of sampling location used the purposive sampling method. Taking the required environmental parameters conducted in situ and sample analysis conducted at Laboratorium Teknik Lingkungan, Universitas Diponegoro. Sampling is done by storing water samples with polyetilene bottles to avoid the entry of sunlight in three repetitions. The result of this research shows that the average  range value of Nitrate content analysis at location is 2,353 – 2,973 mg/L. The average range value of nitrite content 0 – 0,01 mg/L, for the average range value of ammonium content is 14,815 - 18,239 mg / L, while the phosphate content ranged from 0.4767 - 0.05133 mg / L. Based on the nutrient content of nitrate and phosphate fertility waters at the level mesotrophic.
Activity Antioxidant on Pigments of Bacterial Symbionts of Soft Coral From Jepara Sea Masduqi, Ahmad Fuad; Franyoto, Yuvianto Dwi; Kusmita, Lia; Muchlisin, Sakti; Widyananto, Prasetyo Abi; Sulistyani, Sulistyani; Wijayanti, Diah Permata
Indonesian Journal of Natural Pigments Vol 2 No 2 (2020): Agustus 2020
Publisher : Ma Chung Research Center for Photosynthetic Pigments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33479/ijnp.2020.02.02.43

Abstract

Soft corals have been known to produce secondary metabolites, some of which may have anticancer, antifouling, antibacterial and antioxidants activity. It has been suggested that natural products from marine invertebrates have striking similarities to metabolites of their association microorganisms. Symbiont bacteria on soft coral can produce bioactive compounds that play an important role in chemical ecology and as a marine natural product. Marine bacteria associated with soft coral collected from Jepara were successfully isolated on medium ZoBell 2216E and screened to synthesize the pigment. This approach has allowed the use of this organism as an environmentally friendly alternative source of new natural pigment. This study found 25 bacterial isolates from 6 types of soft coral. Out of 25 bacterial isolates, only 3 bacterium, positively contains pigments. Four isolates, PCl 1, PS2 1, and PSa 2. Pigments analysis with UV spectrophotometric method showed the wavelength of pigments were in the range 300-600 nm. Genomic DNA was isolated from these colonies and nested PCR of the DNA was performed to amplify the 16S rDNA. Antioxidant activity was tested with the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. From the results of molecular identification by 16S rDNA method, it was shown that bacterium PCl 1, PS2 1, and PSa 2 was closely related to Pseudomonas stutzeri, Ponticoccus gilvus, Bacillus marisflavi with 99%, 99and 98% homology value. Antioxidant activity is as follows: PCl 1>PS2 1>PSA 2.
Analisa Genetika Gastropoda Nudibranchia dari Pulau Panjang, Jepara Sinulingga, Wilhelmina br; Wijayanti, Diah Permata; Munasik, Munasik; Haryanti, Dwi
Journal of Marine Research Vol 13, No 3 (2024): Journal of Marine Research
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v13i3.38661

Abstract

Gastropoda Nudibranchia memiliki karakteristik insang telanjang atau insang berbulu atau tanduk di punggungnya merupakan anggota Ordo Opisthobranchia, kelompok Gastropoda terbesar dengan anggota lebih dari 3.000 species. Nudibranchia diketahui hidup di Pulau Panjang, salah satu destinasi wisata terkenal di Jepara. Pergerakannya yang lambat dan bentuknya yang menarik, dapat mengganggu keberadaannya di habitat alaminya. Penelitian mengenai Nudibranchia belum banyak dilakukan di Indonesia, termasuk analisa genetiknya. Penelitian ini bertujuan untuk menganalisis keanekaragaman genetik Nudibranchia dari Perairan Pulau Panjang. Analisa DNA dilakukan di Laboratorium Terpadu Universitas Diponegoro, dengan metode PCR-sequencing menggunakan mtDNA-COI (cytochrome oxidase subunit I gene dari DNA mitokondrial). Primer yang digunakan pada penelitian yaitu primer forward: LCOI1490: 5'-GGTCAACAAATCATAAAGATATTGG-3 dan reverse: HCOI2198: 5'-TAAACTTCAGGGTGACC AAAAAATCA-3'. Rekonstruksi pohon filogenetik dan keragaman genetik dilakukan menggunakan software MEGA 6.06 (Analysis of the Evolution of Molecular Genetics). Hasil analisis sampel Nudibranchia berdasarkan susunan DNA mitokondria, ditemukan 2 spesies dengan 8 individu yaitu, spesies Jorunna funebris dan Chromodoris lineolata. Tingkat kesamaan (homologi) dalam analisa BLAST (Basic Local Alignment Search Tool) sebesar 98 % - 100 %. Hasil analisis filogenetik secara menyeluruh memperlihatkan pengelompokan yang terjadi berdasarkan kemiripan genetiknya.Nudibranchia gastropods have the characteristics of naked gills or hairy gills or horns on their backs and are members of the Order Opisthobranchia, the largest group of Gastropods with more than 3,000 species. Nudibranchia are known to live on Panjang Island, one of the famous tourist destinations in Jepara. Its slow movements and attractive shape can disrupt its existence in its natural habitat. Not much research has been done on Nudibranchia in Indonesia, including genetic analysis. This research aims to analyze the genetic diversity of Nudibranchia from Panjang Island waters. DNA analysis was carried out at the Diponegoro University Integrated Laboratory, using the PCR-sequencing method using mtDNA-COI (cytochrome oxidase subunit I gene from mitochondrial DNA). The primers used in the research were forward primer: LCOI1490: 5'-GGTCAACAAATCATAAAGATATTGG-3 and reverse: HCOI2198: 5'-TAAACTTCAGGGTGACCAAAAAATCA-3'. Phylogenetic tree reconstruction and genetic diversity were carried out using MEGA 6.06 (Analysis of the Evolution of Molecular Genetics) software. The results of analysis of Nudibranchia samples based on mitochondrial DNA structure found 2 species with 8 individuals, namely, Jorunna funebris and Chromodoris lineolata species. The level of similarity (homology) in BLAST (Basic Local Alignment Search Tool) analysis is 98% - 100%. The results of a comprehensive phylogenetic analysis show that grouping occurs based on genetic similarity.
Unveiling the DNA Barcoding of Threadfin Breams (Nemipteridae) at Oeba Fish Landing Site and Oesapa Fish Market in Kupang, East Nusa Tenggara Wora, Umbu Domu; Wijayanti, Diah Permata; Widowati, Ita; Ginzel, Fanny Iriany; Nursalim, Nining; Kholilah, Nenik; Bachtiar, Muggi
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 29, No 2 (2024): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.29.2.170-180

Abstract

Threadfin breams (Nemipteridae) are demersal fish species that constitute a significant catch for East Nusa Tenggara fishermen at the Oeba Fish Landing Site and Oesapa Fish Market, where they are landed year-round. Over the years, there has been a noticeable increase in the capture of threadfin breams, raising concerns about the potential impact on their genetic diversity. The ongoing trend could affect the region’s overall population structure of threadfin breams. This study addresses the need to identify threadfin breams in the landing above sites of threadfin breams through molecular analysis of mtDNA COI. The research involved the examination of 24 samples obtained from the Oeba Fish Landing Site and Oesapa Fish Market. The analysis revealed the presence of five distinct threadfin bream species: Nemipterus hexodon, N. japonicus, N. zysron, N. aurora, and Pristipomoides typus. The genetic distance between individual threadfin breams ranged from 0-0.8%, indicating a relatively close genetic relationship within the population. Also, phylogenetic tree reconstruction further delineated five distinct clades based on the species obtained from the samples. Given these findings, the study emphasizes the importance of sustainable threadfin bream capture to preserve genetic diversity. The results underscore the need for ongoing monitoring and management strategies to ensure the threadfin bream population’s long-term health and stability. Additionally, the study suggests that a more in-depth analysis of genetic diversity and the environmental factors influencing this species is warranted for a comprehensive understanding and effective conservation measures.
ASPEK BIOLOGI HIU LANYAM, Carcharhinus sorrah (Valenciennes, 1839), YANG DIDARATKAN DI PELABUHAN PERIKANAN SAMUDERA NIZAM ZACHMAN (PPNSZ) JAKARTA Rizkina, Nurnazmilaila; Wijayanti, Diah Permata; Redjeki, Sri
BAWAL Widya Riset Perikanan Tangkap Vol 16, No 2 (2024): AGUSTUS 2024
Publisher : Politeknik Kelautan dan Perikanan Sorong

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/bawal.16.2.2024.88-99

Abstract

Hiu Lanyam, Carcharhinus sorrah (Valenciennes, 1839) tersebar luas di wilayah perairan Indo-Pasifik, keberadaannya sering ditemukan di hampir seluruh perairan Indonesia. Status konservasi Hiu Lanyam menurut Daftar Merah IUCN dalam kondisi hampir terancam dan diduga populasinya telah menurun. Penangkapan Hiu Lanyam yang semakin tinggi dikuatirkan dapat mengganggu populasinya di alam. Penelitian ini bertujuan untuk mengetahui aspek biologi Hiu Lanyam yang didaratkan di Pelabuhan Perikanan Samudera Nizam Zachman(PPSNZ) Muara Baru, Jakarta. Aspek biologi yang dikaji adalah sebaran ukuran berdasarkan panjang dan berat, rata-rata ukuran pertama kali tertangkap dan pertama kali matang gonad, hubungan panjang berat, nisbah kelamin dan tingkat kematangan klasper. Penelitian ini menggunakan metode deskriptif kuantitatif. Pengambilan sampel dilakukan menggunakan metode acak. Pengukuran panjang tubuh dalam kondisi tanpa kepala (headless). Hasil penelitian diperoleh sampel Hiu Lanyam sebanyak 248 individu. Estimasi panjang total yang didapatkan 61-141 cm (SD : 16,00) dan berat 9,9-14 kg (SD : 2,396). Estimasi panjang maksimum yang didapatkan lebih kecil dari referensi dan hiu yang didaratkan didominasi oleh ukuran muda. Rata-rata ukuran pertama kali tertangkap (Lc) < rata-rata ukuran pertama kali matang gonad (Lm), menggambarkan sebagian besar Hiu Lanyam yang tertangkap belum melakukan pemijahan. Hubungan panjang berat pada kedua jenis kelamin memiliki pola pertumbuhan yang sama, yaitu allometrik positif (b>3).Perbandingan jenis kelamin yang didapatkan ikan betina lebih banyak dari pada jantan, hasil uji Chi-Square menunjukkan nisbah kelamin Hiu Lanyam dalam kondisi seimbang. Pada tingkat kematangan klasper Hiu Lanyam jantan didominasi kategori not full calcification dan estimasi panjang total Hiu Lanyam jantan yang telah matang kelamin >98 cm. Penelitian ini diharapkan dapat menjadi masukan dalam menyusun langkah-langkah pengelolaan dan pembuatan regulasi untuk pembatasan ukuran hasil tangkapan.
Symbiont Types of Scleractinian Corals in Java and Bali Waters, Indonesia Wijayanti, Diah Permata; Indrayanti, Elis; Haryanti, Dwi; Azizah, Mila Amelia Nur; Bhagooli, Ranjeet
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 30, No 1 (2025): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.30.1.71-82

Abstract

Several effects of global warming, particularly coral bleaching, have threatened the symbiotic system of coral reefs. Coral reefs perform a reciprocal system that coincides with coral and their algal symbiont, the environment, and human activities. Nowadays, the system has been threatened by local and global stressors, including global warming.  Understanding the ecological resilience of coral reefs to temperature stress relied heavily on the functional variations between algae symbionts as well as their genotypes. Indonesian coral reefs which play a critical value in supporting the reef fisheries, have been reported to experience bleaching events. However, a few reports were on the symbiont type of Indonesian corals.  This study investigates the Symbiodiniaceae communities of coral hosts of Panjang Island and Karimunjawa Archipelago in Java Sea, and Tulamben Waters, Bali using internal transcribed spacer two (ITS2) amplicon sequencing. In total, 63 sequences out of 119 coral colonies from 16 genera were obtained from Panjang Island, Karimunjawa Archipelago, and Tulamben waters, Bali. All sequences showed approximately 600 bp in the length of the amplified ITS-rDNA region. The BLAST homology analysis of ITS-rDNA region sequences showed that samples matched Cladocopium sp. and Durusdinium sp. with similarities ranging between 96.79-100%. Cladocopium dominated the symbiont type of the Acropora, and Porites, While Durusdinium is the dominant symbiont in the Pavona, Both algal can be found in Pocillopora sp, Montipora sp, Goniastrea sp., and Lobophyllia sp. The occurrence of the two symbiont types suggests that coral species may adopt heat-tolerant Symbiodiniaceae to adapt to the high environmental temperatures. 
Abundance and Diversity of Coral Reef Megabenthos in Karimunjawa Islands, Central Java Wijaya, Aditya Surya; Haryanti, Dwi; Ramadhan, Muhammad Fadhilah; Wijayanti, Diah Permata
Jurnal Kelautan Tropis Vol 28, No 1 (2025): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v28i1.24514

Abstract

Karimunjawa is an archipelago which has a large coral reef area. However, the human and natural factors that put environmental pressure on coral reef ecosystem in recent years have caused the need to update data on the abundance and diversity of megabenthos in Karimunjawa. Data collection was carried out on 9 islands in Karimunjawa out on 30 September-4 October in 2023. The megabenthos data collection method was carried out using the belt transect method with data collection carried out at the family level in the survey area. The method for collecting data on hard coral cover was underwater photo transect (UPT). Megabenthos data analysis was carried out using Microsoft Excel software Coral Point Count with Excel Extensions (CPCe) software for coral cover analysis. Analysis of megabenthos data also includes ecological indices consisting of the Shannon-Wiener diversity index, Pielou evenness index, and dominance index. The relationship between hard coral cover and megabenthos was sought using simple regression analysis. The research results found 8 megabenthos classes and as many as 16 families. The results showed a weak relationship between megabenthos abundance and hard coral cover (r=0.03), but a strong and moderate relationship with the megabenthos ecological index (r=0.434 to 0.568). The regression analysis showed that there was no significant influence between hard coral cover and the abundance and ecological index of megabenthos (P>0.05).  
KELIMPAHAN MIKROPLASTIK PADA KARANG DI WILAYAH ANTROPOGENIK DAN NON-ANTROPOGENIK DI TAMAN NASIONAL KARIMUNJAWA, INDONESIA Muchlissin, Sakti Imam; Sabdono, Agus; Wijayanti, Diah Permata
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 19, No 1 (2023): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijfst.19.1.54-62

Abstract

Produksi plastik semakin meningkat tiap tahunnya yang  didukung pula oleh pola konsumsi dan tren urbanisasi yang terjadi. Diprediksi jumlah sampah plastik di lautan akan meningkat menjadi 250 juta ton dalam beberapa tahun mendatang. Sampah plastik tidak bisa didegradasi secara sempurna dan lambat laun akan berubah menjadi mikroplastik, yakni potongan plastik yang memiliki ukuran kurang dari 5 mm. Hewan karang khususnya pada terumbu karang merupakan organisme penyusun utama terumbu karang. Kemampuan makan karang melalui kolom air secara filter feeding dari polip bersilia menjadikan hewan tersebut salah satu organisme terdampak dari mikroplastik. Tujuan penelitian ini yakni mengetahui tutupan terumbu karang dan genus karang serta kelimpahan mikroplastik pada terumbu karang di BTN Karimunjawa. Metode untuk menentukan tutupan karang adalah Point Intercept Transect (PIT) 50 Meter kedalaman 3-4 M dengan penentuan titik lokasi sampling secara Purposive, dengan pertimbangan lokasi yang banyak mendapatkan aktivitas manusia (antropogenik) dan tidak (non-antropogenik). Terdapat 8 titik sampling yaitu: Pulau Menjangan Kecil, Taka Sendok, Pulau Cemara Kecil, Gosong Cemara, Karang Tengah, Taka Nyamplungan, Pulau Bengkoang dan Pulau Menyawakan. Hasil penelitian menunjukkan bahwa rata-rata tutupan karang adalah 79,71%, sedangkan rata-rata tutupan karang di daerah antropogenik adalah 77,6% dan non-antropogenik adalah 85%. Berdasarkan Keputusan Menteri Lingkungan Hidup dan Kehutanan nomor 4 tahun 2000, tutupan karang tersebut termasuk ke kategori sangat baik. Hasil analisis kelimpahan mikroplastik menunnjukkan rata-rata partikel yang terdapat di karang adalah 27 partikel/50 gram karang atau dengan kata lain ada rata-rata terdapat 540 partikel mikroplastik dalam 1 kg karang utuh. Ada perbedaan cukup signifikan antara kelimpahan mikroplastik di wilayah antropogenik dan non-antropogenik. Kelimpahan mikroplastik di wilayah antropogenik mencapai 146% dibandingkan wilayah non-antropogenik. 
Genetic Analysis on Horseshoe Crab for Phylogenetic Tree Study from Jambi, Bangka Belitung, Central Java, and East Java Province, Indonesia Kholilah, Nenik; Kurniatami, Gabriella Tarida; Kurniasih, Eka Maya; Nursalim, Nining; Janarkho, Galank Fad’qul; Ambariyanto, Ambariyanto; Wijayanti, Diah Permata; Putro, Sapto Purnomo; Cahyani, Ni Kadek Dita
Jurnal Kelautan Tropis Vol 28, No 2 (2025): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v28i2.25923

Abstract

Order Xiphosura, or horseshoe crabs, are rarely found and classified as macrofossils. In Indonesian waters, there are three species: Tachypleus tridentatus, Tachypleus gigas, and Carcinoscorpius rotundicauda. The genetic analysis of their kinship, however, has been extensively unexplored. This study aims to utilize DNA barcoding, focusing on the cytochrome c oxidase subunit I (COI) locus from mitochondrial DNA and investigating the phylogenetic relationships of horseshoe crabs from the four sites (Tanjung Jabung Timur Regency in Jambi, Bangka Regency in Bangka Belitung, Demak Regency in Central Java, and Tuban Regency in East Java). Additionally, the study provides insights into the biodiversity and ecological roles of horseshoe crabs within their ecosystems and surrounding habitats. Through molecular methods, this research focuses on biodiversity analysis through Sanger sequencing and MEGA 11 software for constructing phylogenetic trees and calculating genetic distances. With a total of 22 horseshoe crabs, the DNA samples from four different sites were amplified via Sanger sequencing, targeting the COI locus and analyzing with MEGA 11. The phylogenetic tree analysis revealed two distinct species, Tachypleus gigas, and Carcinoscorpius rotundicauda, exhibiting significant genetic variation between them. A clear genetic separation between the two species was observed. Otherwise, within the C. rotundicauda species, a minor genetic variation was detected between sampling sites. Notably, the genetic composition displayed greater differences between samples from Java and Sumatra compared to differences within each island. The future research should expand the sampling size and include additional genetic markers to provide a more comprehensive understanding of the genetic diversity and evolutionary history of horseshoe crabs in Indonesian waters. Integrating ecological and environmental data could further elucidate the factors driving genetic differentiation and inform conservation strategies.
Blue carbon dynamics and mangrove extent: a case study from the IndonesiaTimor Leste Coastal ecosystem Sitanggang, Wanri; Wijayanti, Diah Permata; Pribadi, Rudhi; Suryono, Chrisna Adhi; Pitaloka, Maria Dyah Ayu; Naitkakin, Egidius; Soares, Daniel Candido Da Costa; Pramudya, Herning; Kiuk, Yosni
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan 2025: Special Issue ICFM
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.0.0.46912

Abstract

Around 20% of mangrove forests in Indonesia are in a degraded condition, which has a negative impact on the potential for blue carbon storage. When mangroves are damaged or degraded, carbon stored in the soil and vegetation can be released back into the atmosphere as CO2, ultimately increasing greenhouse gas emissions and exacerbating climate change. Mangrove forests can store up to four times more carbon per hectare, around 8001,200 tons per hectare, than terrestrial tropical forests. This research was conducted in the Wini mangrove forest area, North Central Timor, East Nusa Tenggara (NTT) which directly borders Timor Leste. The determination of the location of the observation station was based on a map of changes in mangrove forest land use obtained from the Geospatial Information Agency. The parameters measured included organic carbon, mangrove area and sediment type. Organic carbon was analyzed using the Loss on Ignition (LOI) method, and sediment grain size was measured using a particle size analyzer. The results of the study showed that the average organic carbon stock in mangrove forests reached 570,600843,600 tons per hectare, with an average change in the area of mangrove forests from 2013 to 2023 of 181.29 hectares and the type of sediment dominated by sandy mud. This change in area is caused by the conversion of mangrove land into ponds, rice fields, and settlements.Keywords:MangroveCarbonSedimentborder Area