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All Journal METAMORFOSA Journal of Biological Sciences Jurnal Ilmu Dasar BERKALA SAINSTEK Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Jurnal Biodjati Bioma : Jurnal Biologi dan Pembelajaran Biologi JATI EMAS (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat) Jurnal Penelitian Sains dan Teknologi Indonesia Bioma : Jurnal Biologi dan Pembelajaran Biologi
Hidayat Teguh Wiyono
JurusanBiologi FMIPA Universitas Jember
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Mathematics Physics Public Health Social Sciences Other

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Immunogenity of Protein Extract from Salivary Gland of Anopheles aconitus in Malaria Endemic Area Septiawan, Mahful; Budayatin, Budayatin; Wiyono, Hidayat Teguh; Senjarini, Kartika
Jurnal ILMU DASAR Vol 18 No 1 (2017)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (190.462 KB) | DOI: 10.19184/jid.v18i1.2372

Abstract

Although malaria had ever been virtually eradicated from Indonesia but currently malaria is recognized as a serious re-emerging threat to public health. This disease is caused by malaria parasite which is transmitted to human host by Anopheles mosquitoes as main vector. It has been widely observed that saliva of mosquito that transmits disease contains several factors that could enhance pathogen infection. Therefore, it should be possible to control pathogen transmission by vaccinating the host against the molecule(s) in saliva that potentiate the infection. However, immunogenic specific component in mosquitoes vectors of Malaria has not yet been identified so far. The objective of this study are to analyze protein profile of SDS-PAGE and to know the immunogity the protein extract of salivary gland from potential vector of Malaria i.e. An. aconitus We used immunogenic reaction between salivary gland extract of these vectors against pool of human sera which were collected from endemic area. The reaction conducted by the dot-blot analyze. SDS-PAGE studies showed 15 major polypeptide bands of 284, 100, 84, 75, 66, 57, 53, 48, 45, 38, 33, 29, 15, 14, and 11 kDa. The dot-blot studies showed that the protein extract of salivary gland from An. aconitus are immunogenic.
The Diversity of Potential Malaria and Dengue Mosquito Vector from Bangsring Village Wongsorejo District Banyuwangi East Java Azkiyah, Siti Fat'hiyatul; Senjarini, Kartika; Oktarianti, Rike; Wiyono, Hidayat Teguh; Wathon, Syubanul
Jurnal ILMU DASAR Vol 22 No 1 (2021)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v22i1.13601

Abstract

Bangsring village in Wongsorejo regency has been reported as malaria endemic area in Banyuwangi since 2011. Understanding the diversity and behavior of mosquito vector in this area will be very impotant in developing vector control program. The masquitoes were collected by landing collection outside and inside house by human bites, we also observed the area around cattle cage. During observation periods, a total 633 masquitos were collected consist of 44% malaria potential vector (Anopheles spp.) 19% Dengue fever (DF) potential vector (Aedes spp.) and 36% were not belong to both of them. Out of total collected Anopheles spp. mosquitoes about 65% were indentified as An. vagus, 25% were An. indevinitus, 8% were An. vagus (limosus), and only 2% as well as 0,04 % were An. supictus and An. kochi. Meanwhile we found 19% of Aedes sp. were Ae. aegypti and 81% were Ae. albopictus. This study showed that the dominan potential malaria’s vector is An. Vagus and the dominan DF vector is Ae. albopictus. Mostly colleted Anopheles sp. were exophagic and zoophilic with the highest activities between 06.00-08.00 pm. This was in contras to Aedes sp. where mosfly found endophilic and it has 2 hightime of activities Aedes sp. between 06.00- 07.00 AM and 05.00-06.00 PM. Keywords: Dengue, Malaria, diversity, Anopheles, Aedes.
Perilaku Bermain Anak Sapi Peranakan Ongole (PO) di Blok Merak, Kawasan Resort Labuhan Merak Taman Nasional Baluran Sohih, Ahmad Mauludin; Wiyono, Hidayat Teguh; Mahriani, M.
BERKALA SAINSTEK Vol 6 No 2 (2018)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v6i2.9302

Abstract

Perilaku bermain merupakan perilaku yang dilakukan hewan dengan tujuan untuk adaptasi, yaitu sebagai latihan dalam menghadapi predator, latihan dalam menyerang, bertarung, seperti melarikan diri atau menyerang lawannya. Perilaku bermain dapat menjadi indikator kesejahteraan hewan (animal welfare). Penelitian ini dilakukan di Blok Merak, kawasan Resort Labuhan Merak Taman Nasional Baluran. Penelitian ini menggunakan lima anak sapi peranakan ongole (PO). Prosedur penelitian ini yaitu; penentuan sampel anak sapi, pelabelan sampel anak sapi, pengamatan perilaku, dan pengukuran kondisi lingkungan. Metode yang digunakan dalam pengamatan perilaku adalah Focal Animal Sampling. Parameter yang diukur yaitu frekuensi dan durasi perilaku bermain anak sapi PO yang meliputi jumping, bucking, running, mock fighting/butting calf, dan mounting. Data hasil pengamatan perilaku bermain anak sapi PO dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa frekuensi dan durasi perilaku bermain yang meliputi jumping dan bucking tiga kali tiga detik, running 27 kali 71 detik, mock fighting/butting calf 45 kali 434 detik, dan mounting yaitu 88 kali 224 detik. Frekuensi dan durasi masing-masing anak sapi PO yaitu anak sapi A 44 kali 215 detik, anak sapi B 24 kali 115 detik, anak sapi C 42 kali 202 detik, anak sapi D 30 kali 83 detik dan anak sapi E 22 kali 120 detik. Kesimpulan penelitian ini adalah ditemukan lima perilaku bermain yang diamati pada anak sapi peranakan ongole (PO) di Blok Merak, kawasan Resort Labuhan Merak yang digembalakan secara liar di savana Taman Nasional Baluran. Kata Kunci: Perilaku Bermain, Anak sapi Peranakan Ongole (PO), dan Taman Nasional Baluran.
Effect of Baluran Gum Arabic on Blood Glucose Level in Diabetic Rat (Rattus Novergicus ) Wiyono, Hidayat Teguh; Utami, Eva Tyas; Wardhani, Dinna Wahyu Putri
BERKALA SAINSTEK Vol 9 No 2 (2021)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v9i2.22533

Abstract

Diabetes mellitus is a metabolic disease which indicated with increasing blood glucose level. Carbohidrate, protein, mineral and secondary metabolite (alkaloid, tannin and saponin) can be benefical to treat diabetes mellitus. Rats were randomly divided into three groups. First, control group. Second, STZ group, untreated diabetic. Third, STZ+GA 15% group, diabetic treated with GA 15%. Result showed that blood glucose level before STZ induction on control and STZ group within normal range 78,57±11,90 mg/dL and 74,85±6,86 mg/dL. Blood glucose level significantly increase after STZ induction on STZ and STZ+GA 15% group become 375±6,53 mg/dL and 346,42±50,23 mg/dL. Diabetic rat treated with GA 15% revealed decrease in blood glucose level compared to untreated diabetic rat. Blood glucose level on STZ+GA 15% group continuously decrease become 96,42±13,45 mg/dL and 82,14±9,19 mg/dL. In conclusion, GA 15% could reduce blood glucose level on diabetic rat.
Identifikasi Kepik yang Menimbulkan Kerusakan pada Buah Delima (Punica granatum L.) ningsih, Purwati; Wiyono, Hidayat Teguh; Hidayat, Aldin Alfan
Bioma : Jurnal Biologi dan Pembelajaran Biologi Vol. 8 No. 1 (2023): BIOMA: JURNAL BIOLOGI DAN PEMBELAJARAN BIOLOGI
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/bioma.v8i1.378

Abstract

Penelitian ini menggunakan metode deskriptif. Koleksi data sampel dilakukan dengan dua metode yaitu direct sampling dan light trap. Pengambilan sampel kepik dilakukan dengan tiga kali pengulangan. Kepik ditangkap lalu dimasukkan ke dalam botol koleksi berisi alkohol 70%, mounting kering, pelabelan spesimen, proses identifikasi dengan mengamati morfologi spesimen meliputi sayap, tungkai, thorax, antena, dan beak, lalu proses validasi. Hasil identifikasi menunjukkan bahwa kepik (Ordo: Hemiptera) yang ditemukan pada tanaman delima (Punica granatum L.) berasal dari sub-ordo Heteroptera terdiri atas empat famili yaitu Coreidae, Pentatomidae, Scutelleridae, dan Reduviidae. Kepik yang berhasil diidentifikasi sampai tingkatan genus yaitu Leptoglossus, Plautia, dan Halyomorpha. Kepik yang paling banyak menimbulkan kerusakan pada buah delima (P. granatum) adalah Leptoglossus.
Trichosanthes tricuspidata Lour. dari Kabupaten Jember, Jawa Timur Ulum, Fuad bahrul; Wiyono, Hidayat teguh; Setyati, Dwi
Jurnal Penelitian Sains dan Teknologi Indonesia Vol 1 No 1 (2022): Jurnal Hasil Penelitian UNIVERSITAS JEMBER
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LP2M) Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jpsti.v1i1.17

Abstract

Trichosanthes, one of the largest genera of Cucurbitaceae with in species and sub-species diversity offers a broad study for the systematic and taxonomy and also for the phytochemistry and conservation especially for the local specimen. The aims of this study were to determine the specimen and provided the information of medicinal uses and potency, also the conservation strategy. Determination was conducted by literature and confirmation to the experts, while the medicinal and conservation studied with literature review. The taxonomic investigation through the morphological of specimen collected from Jember, East Java represented Trichosanthes tricuspidata Lour. Medicinal potencies of the species were for threatment of imflamation, worm infection, migrain, and fever. Conservation action via habitat protection, species exploration, seed production, breeding activities were purposed for the flora sustainability.
An Extracellular Pectinase from ISH16 Bacteria Isolated Induced by Coffee Pulp Waste Substrate Muzakhar, Kahar; Elida, Farah Salma; Putrasetya, Ramdhan; Siswoyo, Siswoyo; Winarsa, Rudju; Wiyono, Hidayat Teguh
Jurnal Biodjati Vol 7 No 2 (2022): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i2.20279

Abstract

An α-1,4-glycosidic bonds galactoses pectin, mainly composed of a D-galacturonic acid chain, are important biomaterial widely used in industries. Utilizing this material, a bioprocess, including the biocatalysis pectinase, is often needed. Pectinase production was optimized in 7 days SSF at 37°C, and the pectinase activities were daily measured by the method of Somogy-Nelson. The optimum pectinase production was 0.166 U/ml on the fourth day SSF. Purification using open column ion exchange chromatography DEAE cellulose DE-52 resulted in 1030.9 folds of pectinase purity with a yield of 25.9%. The enzyme was at optimal activity at pH six and attended stable in the pH range of 5.5-8, while optimal activity at a temperature of 50°C and was stable in the range of 30-45°C. The pectinase activity increased by 120% with the addition of 10 mM Mg2+, and 95% retained when 10 mM Ca2+ was added. The presence of 10 mM Na+, K+, and Fe2+ resulted in a slight effect of activity at 85%, 83%, and 78%. However, it was strongly inhibited by 10 mM Al3+ and retained 25%. Based on the results above, the microbial utilization of coffee pulp waste by ISH16 bacteria pectinolytic is one opportunity to produce valuable pectinase with low-cost production, so comprehensive examination in large-scale production is needed too. In this paper, all research detail steps were described.
Co-Authors Adrial Adrial Agus Subekti Ali Machrus Amania, Novita Arya Mahdi Azkiyah, Siti Fat'hiyatul Budayatin, Budayatin Dwi Setyati Elida, Farah Salma Eva Tyas Utami Farah Salma Elida Fuad Bahrul Ulum Hidayat, Aldin Alfan Husnatun Nihayah Kahar Muzakhar Kartika Senjarini Kirana Eka Rezki Kusumah, Maulana S. Lelono, Asmoro Mahriani Mahriani Mahriani, M. Nailatur Rifdah Nana Zaimatul Husna Ningsih, Purwati Novita Amania Purwatiningsih Purwatiningsih Putrasetya, Ramdhan Ramdhan Putrasetya Rike Oktarianti Septiawan, Mahful Siswoyo Siswoyo Siswoyo Siswoyo Siti Agustina Wulandari Sohih, Ahmad Mauludin Suratno Suratno Wardhani, Dinna Wahyu Putri Wathon, Syubanul Winarsa, Rudju Yunita Armiyanti