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All Journal VALENSI International Journal of Renewable Energy Development Bionatura Jurnal Ekonomi & Kebijakan Publik Al-Kimia Chimica et Natura Acta ALCHEMY Jurnal Penelitian Kimia Indonesian Journal of Chemistry PENDIPA Journal of Science Education Kimia Padjadjaran
Santhy Wyantuti
FMIPA Universitas Padjajaran
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Education Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics

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Penerapan Desain Eksperimen Plackett-Burman dan Box-Behnken pada Analisis Voltametri Pulsa Diferensial untuk Penentuan Kadar Senyawa Kompleks Gd-DTPA Santhy Wyantuti; Ravenna Aristantia; Yeni Wahyuni Hartati; Husein H Bahti
ALCHEMY Jurnal Penelitian Kimia Vol 16, No 1 (2020): March
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (123.761 KB) | DOI: 10.20961/alchemy.16.1.35166.140-151

Abstract

Gadolinium merupakan salah satu golongan unsur tanah jarang yang menarik karena memiliki sifat paramagnetik yang tinggi. Pengkhelatan gadolinium dengan ligan dietilentriaminpentaasetat (DTPA) menghasilkan senyawa yang berguna dalam bidang kesehatan. Tujuan dari penelitian ini adalah menentukan kandungan unsur tanah jarang gadolinium sebagai kompleks dengan ligan DTPA secara voltametri pulsa diferensial dengan penerapan desain eksperimen Plackett-Burman dan Box-Behnken. Desain eksperimen Plackett-Burman digunakan untuk menyeleksi parameter yang memiliki relevansi positif terhadap respon arus. Parameter yang terpilih kemudian dioptimasi melalui desain eksperimen Box-Behnken sehingga diperoleh kondisi optimum tiap parameter. Parameter yang terseleksi meliputi konsentrasi ligan DTPA, waktu deposisi dan waktu kesetimbangan dengan nilai tiap parameter pada kondisi optimum masing-masing yaitu 150, 13 mgL-1, 60 detik dan 30 detik.  Berdasarkan hasil pengukuran pada kondisi optimum didapat nilai presisi dan akurasi untuk kompleks Gd-DTPA yaitu 99,79% dan 93,42% dengan nilai limit deteksi dan kuantisasi untuk kompleks Gd-DTPA yaitu 3,97 mg/L dan 10,71 mg/L. The Application of Experimental Designs of Plackett-Burman and Box-Behnken in Differential Pulse Voltammetry Analysis for Gd-DTPA Complex Detection. Gadolinium is one of rare earth elements that is interesting because it has high paramagnetic properties. The gadolinium chelating with diethylentriaminpentaacetic acid (DTPA) ligands produces useful compounds in the health field. The purpose of this experiment is to determine amount of gadolinium as complex with DTPA on differential pulse voltammetry by applying Plackett-Burman and Box-Behnken experimental design. The method employed experimental design of Plackett-Burman to select factors, which have positive relevance to response. The selected factors are optimized based on experimental design of Box-Behnken to obtain the optimum condition each factors. The selected factors are ligand concentration, deposition time and equilibrium time with optimum value of 150 mgL-1, 60 s and 30 s, respectively. Based on the result of measurement at optimum condition, the precision and accuracy value for Gd-DTPA complex is 99.79% and 93.42% with detection and quantization limit value for Gd-DTPA complex is 3.97 mg/L and 10.71 mg/L.
A Rapid and Sensitive Diagnosis of Typhoid Fever Based On Nested PCR-Voltammetric DNA Biosensor Using Flagellin Gene Fragment Yeni Wahyuni Hartati; Santhy Wyantuti; M. Lutfi Firdaus; Nurul Auliany; Rini Surbakti; Shabarni Gaffar
Indonesian Journal of Chemistry Vol 16, No 1 (2016)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (333.05 KB) | DOI: 10.22146/ijc.21182

Abstract

Typhoid fever caused by Salmonella typhi is an important issue for public health in the world. Laboratory methods for rapid and sensitive diagnosis are very important for disease management. The purpose of this study was to determine the performance of nested PCR–voltammetric DNA biosensor using flagellin gene (fla) of S. typhi as a marker. The differential pulse voltammetry using pencil graphite electrode was applied to measure the guanine oxidation signal of probes vs synthetic target stDNA and probes vs fla PCR product hybridizations. The probe DNA selectivity was examined by hybridized probes vs non-complementary sequence. The result showed that the first round nested PCR product can not be visualized by agarose electrophoresis, whereas using the voltammetric biosensor methods can be detected both for the first or second round nested PCR product. The average peak current of hybridized probe vs first and second round of PCR product was 2.32 and 1.47 μA respectively, at 0.9 V. Detection of the DNA sequences of the infectious diseases from PCR amplified real sample was also carried out using this voltammetric DNA biosensor methods.
An Electrochemical Aptasensor for the Detection of HER2 as a Breast Cancer Biomarker Based on Gold Nanoparticles-Aptamer Bioconjugates Yeni Wahyuni Hartati; Sari Syahruni; Shabarni Gaffar; Santhy Wyantuti; Muhammad Yusuf; Toto Subroto
Indonesian Journal of Chemistry Vol 21, No 6 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.67124

Abstract

Inaccurate diagnoses contributes to the high mortality rate of breast cancer. Human epidermal growth factor receptor 2 (HER2) is overexpressed in breast cancer tumors at around 20–30%. This study aims to develop an electrochemical biosensor for HER2 based on a gold nanoparticle-aptamer bioconjugate (AuNP@HER2 aptamer) and investigate the interaction between DNA aptamer and HER2 using computational methods. The bioconjugate was synthesized using maleimide and polyethylene glycol as a linker. The –NH2 group of cysteamine that modified the gold electrode can form a covalent bond with the bioconjugate maleimide. The interaction of the bioconjugated aptamer with HER2 was measured electrochemically based on the [Fe(CN)6]3−/4− redox system. The limit of detection, the linear range of HER2, precision, and accuracy in this study were 1.52 ng mL–1, 0.01 to 15.0 ng mL–1, 0.1298, and 94.06%, respectively. The structure of the DNA aptamer was modeled using mFold, Assemble2, and Chimera, with the interaction between the DNA aptamer and HER2 explored by NPDock. The modeling of the aptamer with HER2 showed that electrostatic interactions dominated the attractive forces. The resulting interaction pattern can be used as a template to improve the binding energy of the aptamer, thus providing insight into the development of aptamer-based biosensors.
The Effect of Acetonitrile Solvent on the Quantitative Determination of Europium (III) by Voltammetry and its Optimization using the Box-Behnken Design Uji Pratomo; Ari Hardianto; Yeni Wahyuni Hartati; Husein Hernandi Bahti; Santhy Wyantuti
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 8, No. 1, May 2022
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v8i1.22508

Abstract

There is often a drawback during the determination of Eu in aqueous solvents using the voltammetric method. The current signal from water can reduce that of the element, which causes difficulty while separating the Eu signal from other rare earth elements (REE). Therefore, this study used acetonitrile as a solvent due to its high electrical conductivity and wide potential range. The optimum conditions for the determination of Eu in acetonitrile using the Box-Behnken design include 74.56 seconds deposition time, 0.125 V amplitude modulation, and -2.0 V potential deposition. The platinum electrode's performance showed a recovery value of 98.91% and accuracy and precision (in %RSD) of 96.67% and 1.11%, respectively. Furthermore, detection and quantitation limits of 0.6 mg/L and 5.1 mg/L were recorded from the analysis. It concluded that the differential pulse voltammetry method was applied to determine the presence of Eu in acetonitrile.
Utilization of Steepest Ascent and Box-Behnken Design for Determination of Gadolinium in Acetonitrile by Differential Pulse Voltammetry Santhy Wyantuti; Uji Pratomo; Yeni Wahyuni Hartati; Amelia Shafira; Ari Hardianto; Husein Hernandi Bahti
Indonesian Journal of Chemistry Vol 23, No 5 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.80979

Abstract

Gadolinium (Gd) is an important material for advanced technology; hence, the development of a sensitive and efficient alternative for the Gd-detection method to reduce the dependency on complicated and expensive methods has been massively investigated. Furthermore, the combination of differential pulse voltammetry (DPV) and the experimental design to detect Gd provides a simple, effective, and efficient method. In this study, the Steepest Ascent and Box-Behnken designs were chosen to determine the maximum voltammetry responses. The optimum conditions used for this study showed an amplitude modulation of 0.0884 V, potential deposition of 1.4382 V, and deposition time was 60.3615 s with the obtained recovery value, accuracy, and precision values being 98.37, 95.91, and 5.12% in relative standard deviation (RSD), respectively. Meanwhile, the detection and quantization limit values are 3.46 and 11.53 mg/L, respectively. Under optimum conditions, the presence of Gd in acetonitrile is determined in a mixture with Eu and Sm. Based on the results, the DPV method is capable of determining the presence of Gd in acetonitrile.
Pengaruh Jenis Stripping Agent Pada Fasa Internal Menggunakan Metode Emulsion Liquid Membrane Dalam Pemisahan Logam Pengompleks Purba, Anita Zefanya Marchelina; H Bahti, Husen; P Fauzia, Retna; Pratomo, Uji; Wyantuti, Santhy
Al-Kimia Vol 12 No 1 (2024): JUNE
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/al-kimia.v12i1.46271

Abstract

Heavy metals have a high density, atomic weight, or atomic number. Heavy metals are potentially hazardous to health and the environment and must be separated. Emulsion liquid membrane is a promising technique to remove heavy metal contaminants from industrial and household effluents. Emulsion liquid membrane (ELM) is used to separate heavy metals as it is effective and efficient. ELM involves external, membrane, and internal phases. The internal phase is essential in the separation process as it carries stripping agents to pull metals from the membrane to the internal phase. The choice of stripping agent concentration in the internal phase, such as HNO3, H2SO4, HCl, NaOH, and Na2CO3, affects the extraction efficiency in separating heavying metals using ELM. This article evaluates the effect of stripping agent concentration on extraction efficiency.
Comparative Study of Voltammetric Analysis with UV-Vis Spectrophotometry in Determining the Results of Liquid-Liquid Extraction of Samarium (III) Wyantuti, Santhy; Nurwulanda, Salsha; Mardiah, Najmah; Anggraeni, Anni; Pratomo, Uji; Fauzia, Retna Putri; Bahti, Husein H.
Jurnal Kimia Valensi Jurnal Kimia VALENSI, Volume 10, No. 2, November 2024
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v10i2.38941

Abstract

Rare earth elements (REEs) are garnering significant attention in diverse fields due to their important and fascinating properties. Among these REEs, samarium (Sm) has numerous advantages and benefits. Before using Sm, it must be separated from its natural sources due to the formation of complex compounds with other elements. To achieve this, liquid-liquid extraction emerges as one of the REE separation methods, presenting several advantages, including a streamlined process. Various methods can be used to analyze extraction results, such as ICP-MS and XRF. In comparison, UV-Vis spectrophotometry has significant advantages compared to other methods, it provides a simple approach to determining the level of a substance, and the results given are quite accurate, while differential pulse voltammetry (DPV) is one of the electrochemical analysis methods that is expected to provide faster and accurate analysis results. This study aims to determine the value of analytical parameters, Kd of Sm(III) liquid-liquid extraction results using DPV analysis and UV-Vis spectrophotometry methods. The DPV method yielded LoD 1.24 mg/L,  accuracy 98.39%, and %recovery 106.69%. The extraction data obtained Kd Sm values ranging from 6.0019-7.3860. The UV-Vis spectrophotometry results provided an individual extraction efficiency of Sm(III) of 88.54%. This method obtained LoD 0.71 mg/L, accuracy 96.00%, and %recovery 104.00%.
Penentuan Kadar Asam Folat dalam Konjugat Folat Termodifikasi Silan dengan Spektrofotometer UV Sutanto, Cindy Florencia; Rokhmat, Launa Silky Karenindra; Zahra, Sahlaa Alifah; Hardianto, Ari; Wyantuti, Santhy; Fauzia, Retna Putri
Kimia Padjadjaran Vol 3, No 1 (2024)
Publisher : Kimia Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Konjugasi asam folat (FA) termodifikasi silan merupakan pendekatan yang banyak digunakan untuk meningkatkan spesifisitas sistem penghantaran obat, terutama dalam menargetkan sel kanker yang mengekspresikan reseptor folat secara berlebih. Dalam penelitian ini, dilakukan sintesis, karakterisasi, dan penentuan kadar FA dalam konjugat aminopropil trimetoksisilana–polietilen glikol–asam folat (APTMS-PEG-FA) menggunakan metode spektrofotometri UV-Vis. Konjugasi dilakukan melalui aktivasi gugus karboksilat FA menggunakan EDC dan NHS, dilanjutkan dengan pengikatan terhadap NH₂-PEG-COOH dan APTMS dalam pelarut DMSO. Karakterisasi UV-Vis menunjukkan puncak serapan khas asam folat pada panjang gelombang 288 nm (π→π*) dan 358 nm (n→π*), mengindikasikan keberhasilan konjugasi. Kurva kalibrasi asam folat dibuat pada rentang konsentrasi 0,002–0,010 mM dengan hubungan linear yang sangat baik (R² = 0,99933). Nilai LOD dan LOQ masing-masing sebesar 0,000270 mM dan 0,000820 mM menunjukkan sensitivitas metode yang tinggi. Berdasarkan kurva ini, kadar FA dalam APTMS-PEG-FA diperoleh sebesar 0,33 ± 0,05 mM. Hasil ini menunjukkan bahwa pendekatan konjugasi yang digunakan efektif dalam menghasilkan konjugat dengan integritas kimia dan kuantitatif yang baik, serta berpotensi untuk digunakan dalam aplikasi biomedis, khususnya dalam sistem penghantaran nanopartikel bertarget untuk terapi dan pencitraan kanker.
Co-Authors Amelia Shafira Anni Anggraeni, Anni Annisa Ilma Naviardianti Annisa Ilma Naviardianti Ari Hardianto Ari Hardianto Diana Hendrati Diana Hendrati Diana Rakhmawaty Diana Rakhmawaty Eddy Dikdik Kurnia Eddy, Diana Rakhmawaty Effendi, Syulastri Egista Istioka Fazrin Emma Rochima Erianti Siska Purnamasari Fadhilah, Riana Adisti H Bahti, Husen Hendrati, Diana Husein Hernadi Bahti Husein Hernandi Bahti Lukmanul Hakim M. Lutfi Firdaus M. Lutfi Firdaus, M. Lutfi Mahendra Permadi Mardhotillah, Yulia Mardiah, Najmah Muhammad Yusuf Nurul Auliany Nurwulanda, Salsha P Fauzia, Retna Pratomo, Uji Purba, Anita Zefanya Marchelina Ravenna Aristantia Retna Putri Fauzia Riana Adisti Fadhilah Rini Surbakti Rokhmat, Launa Silky Karenindra Sari Syahruni Shabarni Gaffar Shabarni Gaffar Shabarni Gaffar Shabarni Gaffar Shauvina, Shauvina A Sutanto, Cindy Florencia Toto Subroto Uji Pratomo Yeni Wahyuni Hartati Yeni Wahyuni Hartati Zahra, Sahlaa Alifah