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All Journal Jurnal Kesmas dan Gizi (JKG) Stannum : Jurnal Sains dan Terapan Kimia PRIMER (Prima Medical Journal) Jurnal Pengmas Kestra (JPK) Ranah Research : Journal of Multidisciplinary Research and Development Jurnal Sains dan Kesehatan
Reh Malem Br Karo
Universitas Prima Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Economics, Econometrics & Finance Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences Transportation Other

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Antibacterial Activity of Flavonoid-Rich Fractions Of Citrus Maxima Peel Extract Reh Malem br Karo; Putranto Manalu; Jhon Patar Sinurat
Stannum : Jurnal Sains dan Terapan Kimia Vol 2 No 2 (2020): Oktober 2020
Publisher : Department of Chemistry - Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/jstk.v2i2.1977

Abstract

Natural products can be used as an alternative in the treatment of various diseases such as infectious diseases due to the bioactive compounds contained therein. Moreover nowdays, there are many antibiotic resistance in the treatment of infectious diseases. Citrus maxima is one of the natural products. Citrus maxima have been used for many diseases in traditional medicine.The aim of this study was to investigate the antibacterial activity of flavonoid-rich fractions of citrus maxima peel extract. The bioactive compounds contained in Citrus maxima peel were extracted by maceration method using 96% ethanol solvent. Fractionation was conducted using liquid-liquid extraction using a solvent of water and ethyl acetate obtained ethyl acetate fraction. In this fraction, the TLC test was carried out to confirm the presence of phenolic and flavonoid compounds. The antibacterial activity testing for ethyl acetate fraction against S.aureus and E.coli was determined by disk diffusion method with concentration of 25 ppm, 50 ppm, 75 ppm and 100 ppm. The ciprofloxacin and distilled water were used as positive and negative control, respectively. The result of this study showed that ethyl acetate fraction ( flavonoid-rich fractions) Of Citrus Maxima has potential as antibacterial for bacterial S.aureus and E.coli with medium inhibitory ability in all of concentration ranges. The highest inhibition zone for S.aureus was found at a concentration of 100 ppm while for E.coli was at a concentration of 75 ppm.
FORMULATION FOR MAKING HAND SANITIZER BASED ON DURIAN PEEL EXTRACT (DURIO ZIBETHINUS MURR) IN IMPROVING ANTIBACTERIAL ABILITY Margaretta Olivia Br Manik; Jhon Patar Sinurat; Reh Malem br Karo; Rinaldo Berutu
JURNAL KESMAS DAN GIZI (JKG) Vol. 5 No. 2 (2023): Jurnal Kesmas dan Gizi (JKG)
Publisher : Fakultas Kesehatan Masyarakat Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jkg.v5i2.1665

Abstract

Maceration using ethanol solvent was chosen in extracting durian rind which was then evaporated to obtain 50 grams of durian peel extract. The results of the phytochemical screening test showed that the durian rind contains tannin compounds as evidenced by the addition of Iron (III) chloride reagent to produce a black solution. In addition, durian peel also contains terpenoid compounds which have been proven using the Lieberman Burchard reagent so that a blackish brown ring is produced in the extract. The best hand sanitizer product formulation is 75 mL of 96% ethanol, 2 mL of glycerin, 3 mL of 3% hydrogen peroxide, 10 mL of distilled water and 10 mL of a 6% concentration of durian peel extract solution. The hand sanitizer product has a liquid form, has a clear brownish color, has a distinctive durian rind aroma with a pH around 6 indicating that the hand sanitizer is suitable for use. Concentration of 6% Durian fruit peel hand sanitizer has better antibacterial ability than other concentrations with a large inhibition zone of 12.4 mm which is categorized as a strong level as an antibacterial substance.
SOCIALIZATION OF ANALYSIS OF TOTAL TERPENOIDS FROM MANILTOA GRANDIFLORA (A. GRAY) SCHEFF LEAVES USING TLC METHODS Jhon Patar Sinurat; Reh Malem br Karo; Rinaldo Berutu
JURNAL PENGMAS KESTRA (JPK) Vol 1 No 1 (2021): Jurnal Pengmas Kestra (JPK)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LP2M) Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (313.59 KB) | DOI: 10.35451/jpk.v1i1.738

Abstract

Saputangan leaves contained terpenoids which appeared reddish brown rings when tested using Liebermann Burchard, Salkowski and cerric sulfate. Saputangan leaves were macerated, then partitioned to obtain total terpenoids using a separatory funnel while checking filtrate partition with cerric sulfate. Extracts dissolved with methanol were partitioned with n-Hexane and then partitioned between aquadest and ethyl acetate in a ratio of 1: 1 to obtain 50 g of total terpenoids. TLC analyses was performed on total terpenoids using n-hexane: acetone (80:20 v/v) solvent. The total terpenoids were proved that terpenoids have 11 terpenoids as red stain based on their Rf. Based on the results of tests conducted on participants involved in socialization, it was stated that around 98% of participants had understood terpenoid compounds.
PEMANFAATAN SENYAWA FLAVONOID DALAM EKSTRAK DAUN SAPUTANGAN (Maniltoa grandiflora (A.Gray) Scheff) SEBAGAI SENYAWA ANTIBAKTERI Jhon Patar Sinurat; Reh Malem br Karo
JURNAL PENGMAS KESTRA (JPK) Vol 1 No 2 (2021): Jurnal Pengmas Kestra (JPK)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LP2M) Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (399.151 KB) | DOI: 10.35451/jpk.v1i2.935

Abstract

The Saputangan's leaves extract contained flavonoid compounds which are proven based on the results of screening several flavonoid compound reagents. Flavonoid compounds were examined using FeCl3 to produce a black precipitate, mg metal and concentrated HCl produced an orange color, Alkaline reagents produced a brownish color, Lead acetate produced a cream color and ammonia vapor produced a yellow stain on the TLC plate. As 1000 g of saputangan leaves powder was macerated using methanol as a solvent, followed by the evaporation process in a rotary evaporator and over a water bath to obtain 100 mg of maserate. The maceration process was carried out for several days and the macerate was always checked through flavonoid screening. The flavonoid compounds in the Saputangan leaves could act as an antibacterial with medium strength which produces an average diameter of inhibition zone for S. aureus bacteria of 8.92 millimeters and E. coli bacteria of 9.42 millimeters. After the seminar, participants in the service activities understood that ornamental plants can be used as medicinal plants. In addition, participants also understood the procedures used in the antibacterial test on saputangan leaves.
Formulasi Dan Uji Aktivitas Antibakteri Sediaan Gel Ekstrak Etanol Daun Kerai Payung (Fillicium Decifiens) Terhadap Bakteri Propionibacterium acne dan Staphylococcus epidermis Gadis Minanda Fauziah Purba; Idaman Lase; Reh Malem br Karo; Razoki Lubis; Finna Piska
PRIMER (Prima Medical Journal) Vol. 8 No. 1 (2023): Edisi April
Publisher : Universitas Prima Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34012/pmj.v8i1.3786

Abstract

Sunshade (Fillicium decipiens) is a member of the Sapindaceae family, including some active antibacterial substances. To determine the antibacterial activity of Fillicium decipiens leaf ethanol extract gel against Propionibacterium acne and Staphylococcus epidermis bacteria, this study produced Fillicium decipiens leaf ethanol extract gel preparations using various extract concentrations and other formulations. Fillicium decipiens leaf extraction method by maceration using 96% ethanol. Prepare Fillicium decipiens leaf ethanol extract gel formulation using carbopol 940 base using various concentrations of Fillicium decipiens leaf ethanol extract, each 5%, 10%, and 15%. Gel preparations were tested for the physical properties of the gel, including an organoleptic test, pH test, homogeneity test, and spreadability test. As a result of organoleptic tests, the ethanol extract gel preparation of Fillicium decipiens leaves in each formula, namely F0 (clear color, extract aroma, semi-solid), F1 (greenish-brown color, extract aroma, semi-solid), F2 (greenish-brown color, extract aroma, semi-solid), F3 (greenish brown in color, parasol extract, relatively viscous), for the homogeneity test of the gel preparation formula, it was obtained entirely homogeneous. The formulation that produced the highest inhibition against Propionibacterium acne and Staphylococcus epidermis bacteria was formulation 3 (15% concentration of Fillicium decipiens leaf extract), where the inhibition zone obtained was 17.99 mm for Propionibacterium acne and 17.45 mm for Staphylococcus epidermis.
Uji Efek Antihiperglikimia Fraksi Aktif Ekstrak Etanol Daun Sirsak (Annona Muricata) dan Ekstrak Ikan Gabus (Channa Striata) pada Tikus Razoki Razoki; Cindy Ronaldo Munthe; Reh Malem Br Karo; Muhammad Yunus; Asyrun Alkhaira Lubis
Ranah Research : Journal of Multidisciplinary Research and Development Vol. 6 No. 4 (2024): Ranah Research : Journal Of Multidisciplinary Research and Development (Mei 202
Publisher : Dinasti Research

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.38035/rrj.v6i4.919

Abstract

Tujuan penlitian ini adalah untuk mengetahui efektivitas antihiperglikemia pada tumbuhan daun sirsak (annona muricata) sebagai terapi diabetes mellitus. Ekstrak di maserasi menggunakan pelrut ethanol 96%. Hasil skrining fitokimia menunjukan bahwa pada daun sirsak (annona muricata) mengandung senyawa alkaloid, flavonoid, saponin, tanin dan steroid/triterpenoid. Kemudian di lanjutkan proses fraksinasi ekstrak daun sirsak (annona muricata) untuk memperoleh fraksi aktif ekstrak etanol daun sirsak (annona muricata) dengan partisi cair-cair menggunakan pelarut n-heksan:etanol 96% = 1:1. Pengujian antihiperglikemia menggunakan hewan percobaan tikus puith jantan galur wistar. Konsentrasi kelompok Pada perlaukan pertama tidak diberikan perlakuan atau sebagai kelompok kontrol negatif, kelompok kedua diberikan perlakuan berupa pemberian fraksi ethanol ekstrak daun sirsak dengan dosis 200 mg/kg BB, kelompok ketiga diberikan ekstrak ikan gabus dengan dosis 250 mg/kg BB, kelompok keempat yang diberikan kombinasi fraksi ethanol ekstrak sirsak 200 mg/kg BB dan ekstak ikan gabus 250 mg/kg BB. Kelompok kelima yang diberikan kombinasi fraksi ethanol ekstrak daun sirsak 400 mg/kg BB dengan ekstrak ikan gabus 250 mg/kg BB. Lalu pada kelompok keenam diberikan kombinasi fraksi ethanol ekstrak daun sirsak 800 mg/kg BB dengan eksrak ikan gabus 250 mg/kg BB. Hasil paling besar dalam menurunkan kadar gula darah tikus pada kelompok keenam diberikan kombinasi fraksi ethanol ekstrak daun sirsak 800 mg/kg BB dengan eksrak ikan gabus 250 mg/kg BB dengan nilai rerata penurunan -25,54 %, Kelompok kelima yang diberikan kombinasi fraksi ethanol ekstrak daun sirsak 400 mg/kg BB dengan ekstrak ikan gabus 250 mg/kg BB dengan nilai penurun -3,65%, kelompok kedua diberikan perlakuan berupa pemberian fraksi ethanol ekstrak daun sirsak dengan dosis 200 mg/kg BB dengan nilai rerata penurunan -2,96%. Dan menunjukan bahwa penurunan kadar gula darah terbesar pada tikus jantan galur wistar yang di induksi glukosa 40% yaitu pada pada kelompok keenam diberikan kombinasi fraksi ethanol ekstrak daun sirsak 800 mg/kg BB dengan eksrak ikan gabus 250 mg/kg BB dengan nilai rerata penurunan -25,54 %.
Determination of Total Flavonoid Content of Saputangan Leaves (Maniltoa grandiflora (A. Gray) Scheff) and Its Ability as Antioxidant Jhon Patar Sinurat; Reh Malem Br Karo; Rinaldo Berutu
Jurnal Sains dan Kesehatan Vol. 4 No. 3 (2022): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v4i3.1042

Abstract

Determination of phenolic content using uv-visible and measured IC50 as antioxidant of saputangan leaves. Methods: Screening was used FeCl3, NH3, Alkali and Pb(CH3COO)2. The maceration process was carried out using ethanol as a solvent and soaked for more than 24 hours. The measurement of total flavonoid levels began with determining the maximum wavelength of quercetin, which was 442 nm. The linear regression equation for the resulting quercetin calibration curve was the equation y = 0.0155x + 0.0387 with a correlation value (R2) of 0.9943. The linear regression of antioxidant of saputangan leaves on percentage of inhibition is y = 0.6899x + 1.5616 with a correlation (R2) of 0.9895. The Maniltoa grandiflora (A. Gray) Scheff leaves contained flavonoid compounds which are proven based on the results of screening. Meanwhile, the weight of the extract after partitioning using ethyl acetate and n-hexane was 50 gr. Total flavonoid levels in Maniltoa grandiflora (A. Gray) Scheff leaves were 33.87 mg QE/g extract or 33.87%. IC50 value of 70.2108 ppm which was categorized as an antioxidant at the medium (medium) level. Conclusions: Total flavonoid content was 3.87 mg QE/g extract or 33.87% and categorized as antioxidant on medium level.
Co-Authors Asyrun Alkhaira Lubis Cindy Ronaldo Munthe Finna Piska Gadis Minanda Fauziah Purba Idaman Lase Margaretta Olivia Br Manik Muhammad Yunus Putranto Manalu Razoki Lubis Razoki Razoki Rinaldo Berutu Rinaldo Berutu Sinurat, Jhon Patar