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Aktivitas Antioksidan Jus Stroberi (Fragaria ananassa Duchessne) Terhadap Kadar SGPT, SGOT dan MDA pada Tikus Jantan Galur Wistar yang Diinduksi Isoniazid Ebta Narasukma Anggraeny; Endang Sri Sunarsih; Patricia Sanggita Listyoputri Wibowo; Novi Elisa
JURNAL ILMIAH SAINS Volume 21 Nomor 1, April 2021
Publisher : Sam Ratulangi University

ABSTRAKIsoniazid adalah obat anti tuberkulosis yang digunakan baik sebagai monoterapi atau kombinasi. Penggunaan isoniazid dalam waktu lama dapat menyebabkan hepatotoksik. Hepatotoksik disebabkan oleh hasil metabolisme isoniazid di hepar berupa hidrazin dan asetilhidrazin. Radikal bebas tersebut yang menyebabkan tingginya reactive oxygen species (ROS) didalam tubuh. Tingginya radikal bebas menyebabkan peningkatan kadar SGPT dan SGOT yang merupakan indikator adanya kerusakan hepar. Tingginya radikal bebas dalam tubuh dapat dilihat dari paramter MDA. Hal tersebut dapat diatasi dengan pemberian antioksidan eksogen seperti jus stroberi (Fragaria ananassa Duchessne). Tujuan penelitian ini untuk mengetahui skrinning fitokimia jus stroberi dan pengaruh pemberian jus stroberi terhadap kadar SGPT, SGOT dan MDA pada tikus yang diinduksi Isoniazid. Perlakuan diberikan Â selama 14 hari dengan pembagian kelompok yaitu kontrol normal, kontrol negatif, kontrol positif, dosis 3g/kgBB, 6g/kgBB, dan 9g/kgBB. Pengambilan data dilakukan pada hari 1, hari 15, dan hari 29. Hasil penelitian dapat disimpulkan bahwa jus stroberi dapat menurunkan kadar SGPT, SGOT dan MDA pada tikus yang diinduksi isoniazid dengan dosis efektif sebesar 3g/kgBB tikus.Kata kunci: Isoniazid; Â jus stroberi; MDA; SGPT; SGOT;Effect of Strawberry Juice (Fragraria ananassa Duchessne) against SGPT, SGOT and MDA levels in Isoniazide-Induced Wistar Male RatsÂ ABSTRACTIsoniazid is an anti-tuberculosis drug that is used either as monotherapy or in combination. Prolonged use of isoniazid can cause hepatotoxicity. Hepatotoxicity is caused by the hepatic isoniazid metabolism in the form of hydrazine and acetylhydrazine. These free radicals cause high reactive oxygen species (ROS) in the body. The high level of free radicals causes an increase in SGPT and SGOT levels, which are indicators of liver damage. The high level of free radicals in the body can be seen from the MDA parameter. This can be overcome by giving exogenous antioxidants such as strawberry juice (Fragaria ananassa Duchessne). The purpose of this study was to determine the phytochemical screening of strawberry juice and the effect of giving strawberry juice on the levels of SGPT, SGOT and MDA in rats induced by Isoniazid. The treatment was given for 14 days divided into groups, namely normal control, negative control, positive control, dose of 3g / kg, 6g / kg, and 9g / kg of body weight. Data were collected on day 1, day 15, and day 29. The results of this study concluded that strawberry juice can reduce levels of SGPT, SGOT and MDA in isoniazid-induced rats with an effective dose of 3 g/kgBW rats.Keywords: Isoniazid; Strawberry juice; MDA; SGPT; SGOT

Aktivitas Antihipertensi Ekstrak dan Fraksi-Fraksi Daun Avokad (Persea americana Mill) pada Tikus Jantan dengan Parameter Sistolik dan Diastolik Novi Elisa; Aloysius Barry Anggoro; Erwin Indriyanti
JURNAL ILMIAH SAINS Volume 21 Nomor 2, Oktober 2021
Publisher : Sam Ratulangi University

Penyakit kardiovaskuler yang terjadi di Indonesia berawal dari penyakit menular kemudian beralih menjadi tidak menular, salah satunya hipertensi. Tujuan dari penelitian ini untuk mengetahui skrining fitokimia dan hasil uji KLT ekstrak dan fraksi-farksi daun avokad pada tikus jantan yang dibuat model hipertensi dengan induksi NaCl dan prednison. Â Pengujian ini dilakukan selama 28 hari menggunakan alat Blood Presure Analizer Tail Caff dengan kelompok perlakuan kontrol normal, positif, negatif, ekstrak dosis 75mg/kgbb, 150mg/kgbb, 250mg/kgbb dan fraksi air, etil asetat, n-heksana masing-masing kelompok berisi 5 ekor tikus. Pengukuran tekanan darah dilakukan pada T0 (belum diberi perlakuan), T1, T2, T3 (induksi NaCl dan Prednison), T4 (diberikan dosis ekstrak dan dosis fraksi). Hasil uji skrinning fitokimia positif flavonoid dan uji KLT dimana nilai Rf yang dihasilkan setelah percobaan KLT dengan metode penyinaran UV 254, ekstrak daun alpukat dengan menggunakan baku kuersetin yang dihasilkan Rf 0,75, fraksi air 0,82, n-heksana 0,93, etil asetat 0,82 dari hasil tersebut dapat dibuktikan terjadi penurunan aktivitas tekanan darah. Hasil persentasi penurunan ekstrak dan fraksi-fraksi daun alpukat menunjukkan dosis efektif ekstrak 250mg/kgbb yaitu 5,87/10,96% sig > 0,05 berbeda makna dengan kelompok negatif dan fraksi etil asetat 5.71/14.53%. sig > 0,05 berbeda makna dengan kelompok negatif.Kata kunci: Antihipertensi; daun avokad; diastolik; sistolik; tikusÂ Anti-Hypertension Activity of Avocado (Persea americana Mill) Leaves Extracts and Fractions in Male Rats with Systolic and Diastolic ParametersABSTRACTOne of cardiovascular diseases that occur in Indonesia that start from infectious diseases and then turn into non-infectious diseases is hypertension. This study aimed to determine the phytochemical screening and TLC test results of avocado leaves extract and fractions in hypertension-modeled male rats through induction of NaCl and prednisone. This test was done in 28 days used Blood Pressure Analyzer Tail Caff with normal, positive, negative control treatment groups, extract doses of 75mg/kgbb, 150mg/kgbb, 250mg/kgbb and water, ethyl acetate, n-hexane fractions for each group. Each group contains 5 rats. Blood pressure measurement was done at T0 (untreated), T1, T2, T3 (induction of NaCl and Prednisone), T4 (given extract dose and fraction dose). The results of phytochemical screening test were positive for flavonoids and the TLC test, where the Rf value produced for avocado leaves extract after TLC test with UV 254 irradiation method, using quercetin standard produced was 0.75, water fraction was 0.82, n-hexane was 0.93, ethyl acetate was 0.82. These results then proved with decrease in blood pressure activity. The results of decreases in percentage of avocado leaves extract and fractions showed that the effective dose of extract 250mg/kgBW was 5.87/10,96% sig > 0.05, which was significantly different from negative group and ethyl acetate fraction was 5.71/14.53%. sig > 0.05 significantly different with negative group.Keywords: Antihypertensive; avocado leaves; diastolic; rat; systolic

Antioxidant Activity of Extracts and Fractions of White Cambodian Leaves (Plumeria alba L.) against 1,1-Diphenylpicrylhydrazine (DPPH) Aloysius Barry Anggoro; Erlien Lusy Wijaya; Novi Elisa
Jurnal Ilmiah Sains Volume 22 Issue 2, October 2022
Publisher : Sam Ratulangi University

Free radicals are compounds that play a role in increasing the risk of degenerative diseases that dominate mortality rates in Indonesia. Antioxidants as an antidote to free radicals, one of which is found in frangipani leaves. White frangipani leaves were chosen because they are relatively safe, abundantly available, and have promising potential as antioxidants. This study aims to determine the antioxidant effect of extracts, partitions, fractions from white frangipani leaves, and the thin layer chromatographic profile of the partition with the highest antioxidant activity. The extraction method being used was maceration using methanol followed by partioning the crude extract using n-hexane, ethyl acetate and water solvents. The partition with the lowest IC50 or having the highest antioxidant activity is then fractionated using column chromatography. The determination of antioxidant activity using DPPH method was performed at 516.3 nm. The results showed that Â the ethyl acetate partition had the lowest IC50 among other partitions (94.43 ppm), whereas fraction 2 had the highest antioxidant effect which was followed by fraction 3 and fraction 1 with IC50respectively, 54.11 ppm; 77.79 ppm and 84.07 ppm.Keywords: Antioxidant; chromatography profil; DPPH method; white frangipaniAktivitas Antioksidan Ekstrak dan Fraksi dari Daun Kamboja Putih (Plumeria alba L.) terhadap 1,1-Difenilpikrilhidrazin (DPPH)Radikal bebas merupakan senyawa yang berperan dalam peningkatan resiko penyakit degeneratif yang mendominasi angka kematian di Indonesia. Antioksidan sebagai penangkal radikal bebas, salah satunya terdapat dalam daun kamboja. Daun kamboja putih dipilih karena relatif aman, ketersediaan yang melimpah, dan memiliki potensi sebagai antioksidan yang cukup menjanjikan. Penelitian ini bertujuan untuk menentukan aktivitas antioksidan dari ekstrak, partisi dan fraksi dari daun kamboja putih. Metode ekstraksi yang dipakai adalah remaserasi menggunakan pelarut metanol, dilanjutkan dengan partisi menggunakan pelarut n-heksana, etil asetat dan air. Partisi dengan IC50 paling rendah atau memiliki aktivitas antioksidan tertinggi selanjutnya difraksinasi dengan kromatografi kolom. Penentuan aktivitas antioksidan dengan metode DPPH dilakukan pada panjang gelombang 516,3 nm. Hasil penelitian menunjukkan bahwa partisi etil asetat memiliki IC50 terendah sebesar 94,43 ppm dibandingkan dengan partisi lainnya, sedangkan fraksi 2 memiliki daya antioksidan tertinggi diikuti fraksi 3 dan fraksi 1 dengan IC50 berturut-turut sebesar 54,11 ppm; 77,79 ppm dan 84,07 ppm.Kata kunci: Antioksidan; kamboja putih; metode DPPH; profil kromatografi

Study of antioxidants from herbal plant extracts in vitro Eka Susanti HP; Novi Elisa; Novi - Elisa; Arina Zulfah Primananda; Yustisia Dian Advistasari
Riset Informasi Kesehatan Vol 12 No 1 (2023): Riset Informasi Kesehatan
Publisher : Sekolah Tinggi Ilmu Kesehatan Harapan Ibu Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30644/rik.v12i1.708

Abstract Background : Antioxidants are substances that can prevent the formation of free radical reactions, people in Indonesia use a lot of natural ingredients as traditional medicine, one of which as antioxidants for free radical handling . Free radicals are compounds or molecules that contain one or more unpaired electrons in its outer orbitals. The presence of unpaired electrons causes these compounds to be very reactive looking for partners, by attacking and binding electrons around them so that they can trigger disease. Destination : The purpose of this study was to discuss the antioxidant activity of wormwood, neem, cinnamon, brotowali plants in vitro. Method : The method that can be used in antioxidant test research was the DPPH method in vitro through effective concentration (EC50), identification of phytochemical screening of each extract, and total flavonoid test. Results : The results of the study obtained phytochemical screening of wormwood, neem, cinnamon, brotowali extracts contained secondary metabolites in the form of flavonoids, tannins, saponins, steroids and triterpenoids which have a role as antioxidanttest. Total flavonoid test of wormwood extract had the largest total flavonoid content (110.353 Â± 0.802). The antioxidant test results of each cinnamon extract was 75 ppm, neem extract was 81 ppm, wormwood extract was 479 ppm, brotowali extract was 304 ppm. These results indicated that the effective antioxidant extract was cinnamon extract.

Study of antioxidants from herbal plant extracts in vitro Eka Susanti HP; Novi Elisa; Novi - Elisa; Arina Zulfah Primananda; Yustisia Dian Advistasari
Riset Informasi Kesehatan Vol 12 No 1 (2023): Riset Informasi Kesehatan
Publisher : Sekolah Tinggi Ilmu Kesehatan Harapan Ibu Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30644/rik.v12i1.708

Abstract Background : Antioxidants are substances that can prevent the formation of free radical reactions, people in Indonesia use a lot of natural ingredients as traditional medicine, one of which as antioxidants for free radical handling . Free radicals are compounds or molecules that contain one or more unpaired electrons in its outer orbitals. The presence of unpaired electrons causes these compounds to be very reactive looking for partners, by attacking and binding electrons around them so that they can trigger disease. Destination : The purpose of this study was to discuss the antioxidant activity of wormwood, neem, cinnamon, brotowali plants in vitro. Method : The method that can be used in antioxidant test research was the DPPH method in vitro through effective concentration (EC50), identification of phytochemical screening of each extract, and total flavonoid test. Results : The results of the study obtained phytochemical screening of wormwood, neem, cinnamon, brotowali extracts contained secondary metabolites in the form of flavonoids, tannins, saponins, steroids and triterpenoids which have a role as antioxidanttest. Total flavonoid test of wormwood extract had the largest total flavonoid content (110.353 Â± 0.802). The antioxidant test results of each cinnamon extract was 75 ppm, neem extract was 81 ppm, wormwood extract was 479 ppm, brotowali extract was 304 ppm. These results indicated that the effective antioxidant extract was cinnamon extract.

AKTIVITAS ANTIOKSIDAN DARI EKSTRAK DAUN PEPAYA DAN DAUN CENGKEH SECARA IN VITRO Novi Elisa; Yustisia Dian Advistasari; Ahmad Fuad Masduqi
Jurnal Ilmiah Kesehatan Ar-Rum Salatiga Vol 8, No 1 (2023)
Publisher : STIKES Ar-Rum Salatiga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36409/jika.v8i1.194

Indonesia kaya akan tanaman herbal yang belum banyak dibudayakan sebagai pengobatan, masyarakat memanfaatkan tanaman sebagai pewarna makanan, olahan minuman dan makanan lainnya. Perkembangan zaman masyarakat lebih memilih untuk menggunakan makanan siap saji sehingga kurang dalam memahami khasiat dan kandungan pada makanan tersebut. Radikal bebas memiliki efek yang relatif dan tidak stabil Antioksidan merupakan zat yang dapat mempertahankan dan melindungi tubuh dengan baik oleh penyebab senyawa radikal bebas. Tujuan dalam penelitian ini adalah menguji total flavonoid dan uji antioksidan dari bahan alam ekstrak daun pepaya dan daun cengkeh. Metode penelitian mengguanakan determinasi tanaman dengan pemilihan sempel yang segar, uji total flavonoid yaitu Kandungan flavonoid total ditentukan dengan spektrofotometri UV-VIS dengan panjang gelombang 500 ppm. Sedangkan pada uji antioksidan mengguanakan metode DPPH yang dihasilkan dengan nilai IC50. Hasil penelitian ini uji total flavonoid antara ekstrak dari daun pepaya dan ekstrak daun cengkeh yang dapat memiliki kandungan total flavonoid tebaik adalah ekstrak daun cengkeh dengan total (61.017±0.061), uji antioksidan yang kuat adalah ekstrak daun cengkeh dengan nilai IC50 31.19 %..

Uji Aktivitas Antihipertensi Ekstrak Etanol Daun Matoa (Pometia pinnata J.R. Forster & G. Forster) pada Tikus Jantan yang Diinduksi Angiotensin II dengan Parameter Blood Flow Jaka Seprianto Lepangkari; Novi Elisa; Irvan Charles Seran Klau
Publikasi Penelitian Terapan dan Kebijakan Vol 6 No 1 (2023): Publikasi Penelitian Terapan dan Kebijakan
Publisher : Badan Penelitian dan Pengembangan Daerah Provinsi Sumatera Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46774/pptk.v6i1.532

Hipertensi adalah naiknya tekanan darah sistolik dan diastolik lebih dari 140/90. Salah satu tanaman yang berpotensi sebagai antihipertensi adalah matoa yang mengandung senyawa Quercetin-3-O-rhamnoside dan Kaemferol 3-O-rhamnoside yang diyakini berpotensi sebagai antihipertensi. Pada penelitian ini, peneliti ingin mengetahui persentase penurunan blood flow pada tikus jantan yang diinduksi angiotensin II menggunakan ekstrak etanol daun matoa. Pengerjaan ini dimulai dengan membuat ekstrak etanol dari daun matoa dan membaginya dalam 3 variasi dosis, yaitu 75 mg/kgBB, 150 mg/kgBB, dan 300 mg/kgBB, kemudian diberikan perlakuan ke hewan uji yang telah diinduksi angiotensin II. Hasil penelitian menunjukkan bahwa variasi 3 dosis ekstrak etanol daun matoa dengan dosis 75 mg/kgBB, 150 mg/kgBB, dan 300 mg/kgBB, menunjukkan hasil berbeda. Ekstrak etanol daun matoa dengan dosis 75 mg/kgBB memiliki persentase penurunan 44.47%. Ekstrak etanol daun matoa dengan dosis 150 mg/kgBB memiliki persentase penurunan 53.36%. Ekstrak etanol daun matoa dengan dosis 300 mg/kgBB memiliki persentase penurunan 67.35%. Disimpulkan bahwa ekstrak terbaik yang berpotensi dalam menurunkan sirkulasi aliran darah atau blood flow adalah ekstrak etanol daun matoa dosis 300 mg/kgBB.

Phytochemical Screening and Antioxidant Activity of Strawberry Juice (Fragaria ananassa Duchessne) Against Ureum Level, Creatinin, and Enzyme Catalase Activity In Isoniazid-Induced Wistar Male Rats Endang Sri Sunarsih; Ebta Narasukma Anggraeny; Patricia Sanggita Listyoputri Wibowo; Novi Elisa
STRADA : Jurnal Ilmiah Kesehatan Vol. 9 No. 2 (2020): November
Publisher : Universitas STRADA Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30994/sjik.v9i2.510

ROS increase can causes damage to the kidney so that urea and creatinine level increase in the blood and can be used as a marker of decreased kidney function. Excessive free radicals can cause a decrease in endogenous antioxidant activity, namely the catalase enzyme. This condition can be overcome by given exogenous antioxidants such as strawberry juice (Fragaria ananassa Duchessne). This study aimed to determine the effect of strawberry juice treatment against urea and creatinine levels in male Wistar rats Isoniazid-induced for 14 days divided into groups, namely normal control, negative control, positive control, strawberry juice dose of 3 g/kgBW, 6 g/ kgBW, and 9 g/kgBW. Data were collected on 1st, 15th, and 29th day. The results of the study concluded that strawberry juice had an effect in reducing levels of urea, creatinine and catalase enzyme activity in isoniazid-induced rats with an effective dose of 3g / kgBW

Hypertension Profile of Angiotensin Receptor Blocker From Matoa Leaves Extract (Pometia Pinnata J.R. Foster & G. Foster) In Angiotensin II Induced-Male Rat With Blood Volume Parameter Novi Elisa; Fransiskus Xaverius Sulistiyanto Wibowo Sutardjo; Jaka Seprianto Lepangkari
STRADA : Jurnal Ilmiah Kesehatan Vol. 9 No. 2 (2020): November
Publisher : Universitas STRADA Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30994/sjik.v9i2.595

Hypertension is a problem of cardiovascular disease that occurs in many people. The leaves of matoa, which have another name, Pometia pinnata, are one of the plants used as traditional medicine for the treatment of hypertension. The active compounds of matoa leaves are flavonoids. This study aimed to observe the hypertension profile of the Angiotensin Receptor Blocker class. The test animals used in this study were 45 male Wistar rats. Each group consisted of 5 rats induced by angiotensin II, divided into groups, namely normal control, negative control, positive control, a dose of 75g/kgBW, 150g/kgBW, and 300g/kgBW, and observation were done for 14 days. The hypertension profile observed in this study was the blood volume of rats. The percentage reduction in blood volume of rats given the best matoa leaves extract was 300 mg/kgBW, extract which showed mean difference in negative control group with a significance value of <0.05

The Effect of Pharmaceutical Care against Patient Loyalty with Intervening Variables of Patient Satisfaction at "X" Pharmacy, Sukoharjo Regency Fransiskus Xaverius Sulistiyanto Wibowo Sutardjo; Amor Sofandi; Novi Elisa
STRADA : Jurnal Ilmiah Kesehatan Vol. 10 No. 1 (2021): May
Publisher : Universitas STRADA Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30994/sjik.v10i1.599

This study aimed to determine whether there was an effect of pharmaceutical care on patient satisfaction, to find out whether there was an effect of pharmaceutical care on patient loyalty and to find out whether there was an effect of patient satisfaction in partially mediating the relationship between pharmaceutical care and patient loyalty. Data analysis obtained from testing was carried out by knowing whether there was an effect or not carried out by statistical tests, Anova test using the SPSS 16th version. The results showed that pharmaceutical care had a positive effect on patient satisfaction (sig 0.000 <0.05), pharmaceutical care had a positive effect on patient loyalty (sig 0.000 <0.05), patient satisfaction had a positive effect on patient loyalty (sig 0.000 <0.05), and patient satisfaction has an effect in partially mediating the relationship between pharmaceutical care and patient loyalty (sig 0.000 <0.05).

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