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Aktivitas Antibakteri Actinomycetes yang Diisolasi dari Rizosfer Tanaman Solanaceae Umi Fatmawati; Slamet Santosa; Yudi Rinanto; Riezky Maya Probosari
Jurnal Farmasi Indonesia Vol 11 No 1 (2014): Jurnal Farmasi Indonesia
Publisher : Fakultas Farmasi Universitas Setia Budi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (935.981 KB) | DOI: 10.31001/jfi.v11i1.55

Abstract

Actinomycetes is one type of microorganism that has been widely used for the production of antibiotics as well as a biological control for pests and diseases in plants. In general, many Actinomycetes distributed in soil, water, tissues endophyte, and other natural environments. The study aimed to determine the distribution and diversity of Actinomycetes found in the root zone at Solanaceae plants and to know the isolates of Actinomycetes isolated from the root zone of plants Soalaneceae could inhibiting the growth of bacteria. Actinomycetes isolated from seven soil samples of Solanaceae plants rhizosphere in Selo Boyolali, Colomadu Karanganyar, Gondangrejo Karanganyar, and Wonogiri Jatipurno. The isolates of Actinomycetes tested the antibacterial activity by well diffusion method on media Glycerol-Yeast Extract against four species of pathogenic bacteria Ralstonia solanacearum, Staphylococcus aureus, Bacillus subtilis and Escherichia coli. Results showed that there were 54 isolates of Actinomycetes isolated from the soil samples of Solanaceae plants rhizosphere. Isolates could inhibit the growth of R. solanacearum (29%), S. aureus (24%), B. subtillis (14%), and E. coli (7%). Isolates had the ability to degrade carbohydrate and protein.
Pengujian Resistensi Staphylococcus aureus Hasil Isolasi Udara Ruang Kelas Sekolah Dasar Di Surakarta terhadap Penisilin G, Metisilin dan Vankomisin NATHALIA KURNIA ASTRIANI; TITIK SUNARNI; YUDI RINANTO
Jurnal Farmasi Indonesia Vol 9 No 1 (2012): Jurnal Farmasi Indonesia
Publisher : Fakultas Farmasi Universitas Setia Budi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1020.08 KB) | DOI: 10.31001/jfi.v9i1.727

Abstract

Staphylococcus aureus is the bacteria that cause serious infections. This is supported by the presence of Staphylococcus aureus resistance to antibiotics. Air condition in the Elementary School classroom as a factor enabling the spread of infectious diseases in which elementary school is a community that consist of children aged between 6-12 years on average has not been able to be independent terms of cleanliness. Especially with irrational or excessive use of antibiotics in children appears to be more frequent and increasingly alarming. This research begins by sampling the air of bacteria with medium Vogel Johnson Agar to be opened for 30 minutes in elementary school classrooms. Samples obtained from nine elementary schools in Surakarta. Incubation at 37ºC for 24 hours. Identification test carried Staphylococcus aureus bacteria and then isolated. Staphylococcus aureus obtained is used as the test bacteria to antibiotics penicillin G, methicillin and vancomycin. The result of this study was from 27 point in nine elementary school there are 20 point containing Staphylococcus aureus. Based on sensitivity tests there are 30 % resistant to penicillin G, 5 % for methicillin and not anything for vancomycin. Mean while based on penicillinase test there are 6 point that Staphylococcus aureus produces penicillinase enzyme.
Co-Authors Baskoro Adi Prayitno Fransiska Leviana NATHALIA KURNIA ASTRIANI Nina Teja Suryani Nuri Aslami Nurmiyati Nurmiyati Resty Hermita Riezky Maya Probosari Salis Khoirun Nisa Slamet Santosa Suciati Suciati Titik Sunarni Umi Fatmawati