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All Journal Proceeding of Mulawarman Pharmaceuticals Conferences Journal of Tropical Pharmacy and Chemistry Jurnal Sains dan Kesehatan
Wahyu Widayat
Fakultas Farmasi
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology

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Isolation and Characterization Endophytic Fungal Isolate from Peronema canescens Jack Leaf and Coptosapelta tomentosa Val. K. Heyne Root Arsyik Ibrahim; M. Arifuddin; Wisnu Cahyo P; Wahyu Widayat; Mahfuzun Bone
Journal of Tropical Pharmacy and Chemistry Vol. 4 No. 5 (2019): J. Trop. Pharm. Chem.
Publisher : Faculty of Pharmacy, Universitas Mulawarman, Samarinda, Indonesia, 75117, Gedung Administrasi Fakultas Farmasi Jl. Penajam, Kampus UNMUL Gunung Kelua, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jtpc.v4i5.169

Abstract

Has been done Isolation, Characterization and Secondary Metabolite Endophytic Fungal Isolate from Peronema canescens Jack Leave and Coptosapelta tomentosa Valeton K. Heyne Root. The aim of this research is to know the number of fungal isolates, chromatogram profile and secondary metabolite group of endophytic fungal isolates from P. canencens leaves and C. tomentosa root. Characterization of endophytic fungal isolates was done macroscopically and microscopically. Identification of secondary metabolites endophytic fungal isolates were performed by chemical reaction test and TLC (Thin Layer Chromatography) method with specific spray reagents. The data of this study were obtained based on the number of endophytic fungal that can be isolated, observing macroscopic and microscopic morphological profiles, chromatogram profile and secondary metabolites of each endophytic fungal isolated. The results showed that endophytic fungal that can be isolated from P. canencens leaves four isolates, and two isolates from C. tomentosa root. Morphological profile macroscopic endophytic fungal of the six isolates showed a greenish-colored colony, white gray, clear black. Microscopic profile of each fungal isolate having spores, sprangiosphora, sporangium, conidia, hyphae and stolon. The identified secondary metabolites are: alkaloids, terpenoids, and flavonoids, and polyphenols.
Effect of Media Types on the Growth of Callus Culture in Kumis Kucing Orthosiphon aristatus (Blume) Miq. Wahyu Widayat; Muhammad Satyo Pradana; Mirhansyah Ardana
Journal of Tropical Pharmacy and Chemistry Vol. 5 No. 1 (2020): J. Trop. Pharm. Chem.
Publisher : Faculty of Pharmacy, Universitas Mulawarman, Samarinda, Indonesia, 75117, Gedung Administrasi Fakultas Farmasi Jl. Penajam, Kampus UNMUL Gunung Kelua, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jtpc.v5i1.210

Abstract

The lack of conventional availability of plant Kumis Kucing makes tissue culture techniques used as a solution to overcome this problem. In tissue culture, media is a major factor in producing a good crop of plantlets. Media Murashige & Skoog (MS), Lloyd & McCown Woody Plant (WPM) media was used in the induction of Orthosiphon aristatus (Blume) Miq cat whiskers callus culture, in this study also used growth regulators in the form of 2,4-D added to each-individual media. The results showed the best callus growth occurred in Murashige & Skoog (MS) media compared to the Lloyd & McCown Woody Plant (WPM) media, where the callus produced was 3.28 g on MS media.
Formulasi Infusan Daun Sirih Merah (Piper crocatum) sebagai Gel Antiseptik Tangan Wisnu Cahyo Prabowo; Wahyu Widayat; Synthia Defriana
Jurnal Sains dan Kesehatan Vol. 1 No. 10 (2018): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v1i10.59

Abstract

Melimpahnya sumberdaya alam Indonesia khususnya dalam pemanfaatannya sebagai bahan obat maupun kosmetik. formulasi sediaan obat tidak hanya mengandalkan bentuk fisik sediaan yang baik tetapi juga efektivitas/khasiat yang baik. Sasaran penelitian ini bertujuan untuk memperoleh sediaan gel antiseptik dengan bahan aktif daun sirih merah yang efektif membunuh mikroba ditangan serta daya hambat terhadap beberapa mikroba uji yang patogen. Bahan segar diekstraksi kemudian diformulasi dengan basis gel. Sediaan gel dengan berbagai konsentrasi diuji stabilitas berdasarkan penyimpanannya. Efektifitas sediaan tersebut di uji terhadap mikroba tangan dan beberapa mikroba patogen. Potensi efektifitas sediaan diperoleh dengan membandingkan dengan sediaan kontrol berbahan aktif etanol dan triklosan. Sediaan gel antiseptik ekstrak daun sirih merah baik stabil pada penyimpanan suhu 18-27oC dengan pH 5,5 dan memiliki warna bening kekuningan. Konsentrasi sediaan gel antiseptik efektif pada 25% terhadap mikroba tangan yang setara dengan handsanitizer alkohol, sedangkan pada konsentrasi efektif 15% terhadap Candida albican; konsentrasi 20% terhadap Candida utilis; dan 25% terhadap Vibrio cholera, keseluruhannya setara dengan handsanitizer alkohol.
Profil Pengobatan dan DRP’S pada Pasien Ganguan Lambung (Dyspepsia, Gastritis, Peptic Ulcer) Di RSUD Samarinda Wahyu Widayat; Iffah Karina Ghassani; Laode Rijai
Jurnal Sains dan Kesehatan Vol. 1 No. 10 (2018): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v1i10.100

Abstract

Gastric is the gastrointestinal organ which located after the esophagus before the duodenum and has a function in the process of food anabolism. Gastric disorders can be caused by Helicobacteria pylori infection, eating habit, stress, and side effects of NSAID drugs with the number of patients increasing each year. The purpose of this study is to analyze the characteristics and patterns of treatment in RSUD Samarinda to patients who have gastric disorders and DRP's profile. The research method used was non experimental. The data obtained were taken prospectively and the sampling technique used was incidental sampling. The research population were patients with gastric disorders (dyspepsia, gastritis, peptic ulcer disease) who were undergoing therapy at RSUD Samarinda. The results showed that patients with gastric disorders were dominated by women (55.7%), elderly 46-55 years old (36.5%), elementary school educational background (40.4%), private employees(36.5%), taking NSAID drugs (59.6%) and smoke (65.2%). Patients often eat spicy (82.7%), drink coffee (53.8%), and do not eat regularly (17.3%). Meanwhile, the patients receiving treatment pattern at the hospital were given treatment pattern 1 (36,5%), treatment pattern 4 (57,7%), treatment pattern 13 (58,8%). Presumptions towards DRPs profile is moderate drug interactions 80.6% and minor drug interactions 12.8%. As much of 23% of the patients showed certain indication but did not given any therapy, while as much of 5.7% of the patients were getting therapy without showing certain indication. As much of 65.4% patients take herbs (Turmeric and Curcuma) Keywords: DRP’s, Dyspepsia, Gastritis, Peptic Ulcer
Analisis Interaksi ?-Amilase Bacillus licheniformis (BLA) dan Mutannya (MTBLA) dengan Maltoheptaosa pada Suhu Tinggi menggunakan Metode In Silico: Interaction Analysis of ?-Amylase Bacillus licheniformis (BLA) and its Mutant (MTBLA) with Maltoheptaose at High Temperature using In Silico Method Annisyaban Fatiha Azzahra; Regaputra Satria Janitra; Wahyu Widayat; Farhan Azhwin Maulana; Safri Ishmayana; Muhammad Yusuf
Jurnal Sains dan Kesehatan Vol. 5 No. 6 (2023): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v5i6.2013

Abstract

?-Amylase is an enzyme that hydrolyzes starch into oligosaccharides used in the food and health industry sector. Hydrolysis products such as maltopentose can be a high-nutrition source for patients with kidney failure and calorie deficit. Therefore, increasing the thermostability of enzymes needs to be done to meet industrial criteria. In this study, we studied the effect of the Bacillus licheniformis ?-amylase (BLA) mutation on its activity at 298 and 373 K in silico through enzyme-substrate interaction analysis. Maltoheptaose was used as a model substrate. At 373 K, the Asn190Phe mutation plays a direct role in forming interactions with the substrate while the Gln264Ser mutation does not play a direct role. At 298 K the Asn265Tyr mutation plays a direct role in the enzyme-substrate interaction. In this study, we found a relationship between enzyme-substrate interaction and activity, the most determining factor being the interaction with the catalytic residue. At 298 K, the total interaction of the mutant BLA (MTBLA)-maltoheptaose was slightly stronger than that of BLA-maltoheptaose. However, the interaction of maltoheptaose with catalytic residues in BLA is stronger than in MTBLA. This is in agreement with the previous experiment at 298 K. A similar pattern is found at 373 K. Thereby, MTBLA activity at 373 K is also not more improved than BLA. Keywords:          ?-Amylase, activity, thermostability, Bacillus licheniformis, In silico   Abstrak ?-Amilase merupakan enzim yang menghidrolisis pati menjadi oligosakarida yang digunakan di sektor industri makanan dan kesehatan. Produk hidrolisis seperti maltopentosa dapat menjadi sumber makanan tinggi nutrisi untuk pasien gagal ginjal dan defisit kalori. Oleh sebab itu, peningkatan termostabilitas enzim perlu dilakukan untuk memenuhi kriteria industri. Pada penelitian ini kami mempelajari pengaruh mutasi ?-amilase Bacillus licheniformis (BLA) terhadap aktivitasnya pada suhu 298 dan 373 K secara in silico melalui analisis interaksi enzim-substrat. Maltoheptaosa digunakan sebagai model substrat. Pada suhu 373 K, mutasi Asn190Phe berperan langsung membentuk interaksi dengan substrat sedangkan mutasi Gln264Ser tidak berperan langsung. Pada suhu 298 K mutasi Asn265Tyr berperan langsung terhadap interaksi enzim-substrat. Pada penelitian ini, kami menemukan hubungan antara interaksi enzim-substrat terhadap aktivitas, faktor yang paling menentukan adalah interaksinya dengan residu katalitik. Pada suhu 298 K, total interaksi mutan BLA (MTBLA)-maltoheptaosa sedikit lebih kuat dibandingkan BLA-maltoheptaosa. Namun, interaksi maltoheptaosa dengan residu katalitik pada BLA lebih kuat daripada MTBLA. Hal ini bersesuaian dengan eksperimen sebelumnya pada suhu 298 K. Pola yang mirip terlihat pada 373 K, sehingga aktivitas MTBLA pada suhu 373 K juga tidak lebih baik daripada BLA. Kata Kunci:         ?-Amilase, aktivitas, termostabilitas, Bacillus licheniformis, In silico
Co-Authors Adam M. Ramadhan Annisyaban Fatiha Azzahra Arsyik Ibrahim Deya Apriliana Faisal Riyanda Farhan Azhwin Maulana Fidhia Rara Lande Hidayat, G.A Hidayat, G.A Iffah Karina Ghassani Laode Rijai Laode Rijai M Arifuddin M. Arifuddin Mahfuzun Bone Mirhansyah Ardana Mirhansyah Ardana Muhammad Satyo Pradana Muhammad Yusuf Nisa Naspiah Nurul Musdalifah Aprilia Regaputra Satria Janitra Rolan Rusli Rullah Hermanda Safri Ishmayana syarifah maryam alaydrus Synthia Defriana Vendryca Reckow Wisnu Cahyo P Wisnu Cahyo Prabowo Wisnu Cahyo Prabowo Yurika Sastyarina