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Ekstrak Moringa oleifera Sebagai Antiseptik Terhadap Escherichia coli dan Streptococcus pyogenes Menggunakan Metode Percentage Kill Conny Riana Tjampakasari; Ariyani Kiranasari; Alfia Chairunnisa; Fatindhiya Mahira Hilmy
The Indonesian Journal of Infectious Diseases Vol. 8 No. 2 (2022): The Indonesian Journal of Infectious Disease
Publisher : Rumah Sakit Penyakit Infeksi Prof Dr. Sulianti Saroso

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32667/ijid.v8i2.145

Abstract

Latar belakang: Kandungan kimia daun kelor (M.oleifera) menunjukan sifat antibakteri terhadap bakteri Gram positif dan Gram negatif dengan menghambat sintesis dan metabolisme DNA serta menghancurkan dinding sel. Penelitian ini bertujuan untuk mengetahui signifikansi kemampuan ekstrak daun M.oleifera sebagai antiseptik terhadap E. coli dan S. pyogenes. Metode: Uji percentage kill dilakukan untuk mengetahui persentase kematian bakteri setelah kontak dengan M. oleifera pada menit ke 1, 2 dan 5. Variabel yang digunakan yaitu kontrol dan perlakuan yang dilakukan secara bersamaan. Uji dinyatakan memenuhi standar bila memberikan hasil ≥90% untuk setiap waktu kontak. Hasil: Uji terhadap E. coli pada menit ke 1, 2 dan 5 menunjukkan hasil masing-masing 93.41%, 94.14%, dan 96.87%, sedangkan terhadap S. pyogenes masing-masing 73.27%, 83.15% dan 94.19%. Terhadap S. pyogenes pada menit ke-5 memenuhi standar. Kesimpulan: M. oleifera efektif mengeliminasi E. coli karena nilai percentage kill untuk semua waktu kontak ≥90% dengan nilai tertinggi pada menit ke-5 (96.87%), sedangkan terhadap S. pyogenes ekstrak daun M. oleifera menunjukkan aktifitas mikroba yang baik pada waktu kontak 5 menit (94.19%).
TEKNIK PRESERVASI BIAKAN JAMUR Conny Riana Tjampakasari; Regita Aulia Rosalina; Chrecentia Hanna Swestikaputri
Journals of Ners Community Vol 13 No 2 (2023): Journals of Ners Community
Publisher : Fakultas Ilmu Kesehatan Universitas Gresik

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55129/jnerscommunity.v13i2.2579

Abstract

Teknik preservasi biakan jamur adalah hal yang penting dilakukan guna menjaga kelestarian biakan. Tujuan preservasi biakan jamur adalah memperoleh biakan dengan viabilitas masih baik dan tidak mengalami perubahan baik secara genetik maupun fenotipe meskipun disimpan dalam waktu tertentu. Preservasi jamur berdasarkan lama penyimpananya dibedakan menjadi 2, yakni preservasi jangka pendek dan jangka panjang. Preservasi jangka pendek dilakukan untuk keperluan rutin penelitian yang disesuaikan dengan kegiatan program atau proyek tertentu sedangkan preservasi jangka panjang dilakukan dalam kaitannya dengan koleksi dan konservasi plasma nutfah jamur, sehingga apabila suatu saat diperlukan, dapat diperoleh kembali. Sangatlah penting untuk memilih teknik preservasi yang tepat agar kultur jamur yang dimiliki tetap lestari, tidak hilang akibat kematian ataupun terkontaminasi. Pilihan teknik preservasi tergantung pada pentingnya koleksi, karakteristik metode, ketersediaan peralatan dan biaya pemeliharaan
Pseudomonas aeruginosa biofilm formation and its resistance to beta-lactam antibiotics Cynthia Gozali; Conny Riana Tjampakasari
Bahasa Indonesia Vol 22 No 2 (2023): Damianus Journal of Medicine
Publisher : Atma Jaya Catholic University of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25170/djm.v22i2.3928

Abstract

ABSTRACT Introduction: An opportunistic pathogen, Pseudomonas aeruginosa is a Gram-negative bacteria that causes acute and chronic human infections. By adhering to appropriate surfaces and creating a biofilm matrix, Pseudomonas aeruginosa has a high-resistance structure. Due to biofilm resistance mechanisms, this bacterial biofilm may increase natural antibiotic resistance. Antibiotics have difficulty penetrating the exopolysaccharide matrix that makes up the biofilm structure (Psl, Pe, alginate, and eDNA). Alginate is involved in generating mucus, and Psl and Pel components are implicated in biofilm development and antibiotic resistance. Due to the connection between biofilm production and antibiotic resistance, Pseudomonas aeruginosa involves mechanisms of beta-lactam antibiotic resistance. Objective: On this basis, learning more about the connection between Pseudomonas aeruginosa bacteria's ability to form biofilms and their resistance to beta-lactam antibiotics is essential. Methods: We use literature methods from various literature on the biofilm formation of P. aeruginosa and its resistance to beta-lactam antibiotics, including research papers and studies with no restrictions types of studies included in this article. Discussion: Pseudomonas aeruginosa has intrinsic and adaptive antibiotic resistance mechanisms. Decreased membrane permeability, the production of enzymes resistant to antibiotics, chromosomal changes, and horizontal gene transfer from other bacteria contribute to antimicrobial resistance. Beta-lactam antibiotic resistance mechanisms are known to be developed by Pseudomonas aeruginosa. The resistance characteristics of Pseudomonas aeruginosa may change phenotypically due to biofilm development. This article will be helpful in future research on Pseudomonas aeruginosa therapy and medication.  
Kultur Slide Sebagai Metode Mikroskopik Tidak Langsung untuk Identifikasi Jamur Kapang Conny Riana Tjampakasari; Riani Agustini; Ichwan Baihaki; Shoffiana Noor; Arleni Bustami
Jurnal Sehat Indonesia (JUSINDO) Vol. 6 No. 01 (2024): Jurnal Sehat Indonesia (JUSINDO)
Publisher : Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59141/jsi.v6i01.75

Abstract

Teknik kultur slide merupakan pengamatan mikroskopis tidak langsung yang digumnakan untuk mengidentifikasi isolat jamur berdasarkan hifa yang dibentuk. Dengan metode ini pemeriksaan secara rinci jamur berfilamen tetap menjadi salah satu prosedur andalan untuk identifikasi morfologi jamur di laboratorium mikologi diagnostik di seluruh dunia. Identifikasi yang akurat berdasarkan visualisasi detail mikroskopis seperti bentuk, susunan konidia yang halus dan perlekatan konidia dengan sel konidia sangat penting dan memiliki pengaruh pada manajemen klinis mikosis. Pada pemeriksaan ini telah berhasil dilakukan identifikasi Aspergillus mengunakan teknik kultur slide. Diperoleh gambaran struktur hifa, konidiofora, vesikel, sterigmata dan konidia diatasnya. Metode ini merupakan teknik identifikasi yang paling umum untuk identifikasi jamur kapang patogen. Teknik ini relatif lebih tahan lama untuk menyimpan gambaran morfologi dibandingkan metode selotip. Walaupun demikian, perlu dilakukan modifikasi metode untuk meningkatkan kegunaannya dan mempersingkat waktu untuk mengidentifikasi.
Rickettsia as an agent of neglected Rickettsia infection Tjampakasari, Conny Riana; Kiranasari, Ariyani; Abas, Ghina Mutiara
Journal of Clinical Microbiology and Infectious Diseases Vol. 4 No. 1 (2024): Available online: June 2024
Publisher : Indonesian Society for Clinical Microbiology (Perhimpunan Dokter Spesialis Mikrobiologi Klinik Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51559/jcmid.v4i1.42

Abstract

Rickettsia is an obligate parasite of certain arthropods (especially ticks, mites, and ticks) and can cause serious diseases. OmpA dan OmpB is a surface protein that facilitates Rickettsia’s fast attachment and entry into the endothelium. Clinical symptoms are usually characterized by self-limited acute fever in both humans and other animals. Diseases caused by Rickettsia are endemic in almost all around the world, including Indonesia. Rickettsia diseases include epidemic typhus, murine typhus, scrub typhus, and spotted fever. Culture, nucleic acid amplification, and serological tests can be used as diagnostic examinations. The serological test is the gold standard of Rickettsial diagnostic, even though molecular tests have been developed. Diagnostic limitations mean that rickettsiosis is often overlooked as a cause of infection. Rickettsia eradication was carried out by giving tetracycline, chloramphenicol, and rifampin. Vaccines are not yet available so prevention and appropriate use of antibiotics based on the diagnostic examination will determine the success of eradication.
Bacterial Filtration Efficiency Stevina, Lany; Tjampakasari, Conny Riana
Journal of Clinical Microbiology and Infectious Diseases Vol. 4 No. 1 (2024): Available online: June 2024
Publisher : Indonesian Society for Clinical Microbiology (Perhimpunan Dokter Spesialis Mikrobiologi Klinik Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51559/jcmid.v4i1.49

Abstract

Background: The specifications of medical masks are different from those of non-medical masks although they may appear similar. Currently, the Indonesian Government through the Ministry of Health has made efforts to tighten supervision of the quality of medical mask products circulating in the community. Bacterial Filtration Efficiency (BFE) testing is one of the requirements for medical masks to obtain circulation permit from the Indonesian Ministry of Health. Medical masks can be considered good if they have a minimum bacterial filtration efficiency of 95%. If the percentage of BFE result is greater, then the ability of the mask to filter bacteria is also greater. This review aims to provide information on the performance of various types of medical masks through BFE testing and the relationship with the characteristics of the materials used. Method: Relevant articles were obtained from PubMed, Science Direct, Research Gate, Google Scholar, and springer databases and then filtered. Sixteen articles were reviewed and included in this study to achieve the study objective. Result: There are various types of medical masks including surgical masks and N-95 respirator masks. One of the requirements for medical masks is the ability to filter or (BFE) test. Factors that affect the filtration ability of masks include the type of material used and the method of making the mask. Conclusion: Testing the filtration efficiency of medical masks can be done through the Bacterial Filtration Efficiency (BFE) test. Good medical masks must have a minimum bacterial filtration efficiency of 95%.
Function of Moringa oleifera Lamk Leaf Extract as an Antiseptic for Pseudomonas aeruginosa and Staphylococcus aureus using Percentage Kill Method Kiranasari, Ariyani; Tjampakasari, Conny Riana; Putri, Mariza Nabila; Pratama, Vanessa Maritza
Jurnal Kedokteran Meditek Vol 30 No 3 (2024): SEPTEMBER
Publisher : Fakultas Kedokteran Universitas Kristen Krida Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36452/jkdoktmeditek.v30i3.2864

Abstract

Moringa oleifera Lamk leaf extract has an antimicrobial properties especially against fungal, parasite, Gram positive and negative bacteria by inhibiting DNA synthesis and metabolism an damaging cell walls. The aim of this study was to determine whether M. oleifera Lamk leaf extract is effective as an antiseptic against P. aeruginosa and S. aureus bacteria. Percentage Kill was used in the study to determine the percentage of  bacteria death after contact with M. oleifera Lamk leaf extract in the 1st, 2nd and 5th minutes against the control and treatment simultaneously. The test is considered to meet the standard if each contact gives a result of ≥90%. For  discovered percentage kill to P. aeruginosa bacteria in the 1st, 2nd and 5th minutes each test showed a yield of 27.12%. , 47.01%, 57.7%. In the third time of  P. aeruginosa did not reach the standard. Whereas in S. aureus bacteria, each test showed a yield of 92.36%, 95.58% and 96.45%, where it was seen that all results reach  the standards criteria of ≥90%.  M. oleifera Lamk leaf extract was not effective in eliminating P. aeruginosa for all contacts because the standard value was below 90%. Whereas for S. aureus bacteria, M. oleifera Lamk leaf extract was very effective in eliminating bacteria for all contact times ≥90% with the highest value at the 5th minutes (96.45%).
INHIBITION OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) BIOFILM: THE ESSENTIAL ROLE AND POTENTIAL USAGE OF BACTERIOCINS Febrianti, Tati; Conny Riana Tjampakasari
Journal of Vocational Health Studies Vol. 8 No. 1 (2024): July 2024 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V8.I1.2024.68-77

Abstract

Background: The potential of Methicillin-Resistant Staphylococcus aureus (MRSA) to develop biofilms and its resistance to antibiotics become major worldwide issue. Complementary anti-microbial strategies have been used recently, in particular for the treatment of MRSA biofilm-associated resistance. Purpose: To review the potential, essential role, and mechanism of bacteriocin that can inhibit MRSA biofilms. The review was conducted by searching and analyzing published articles from Elsevier, ProQuest and PubMed database. Review: Globally, the incidence of MRSA in 85 countries based on WHO surveillance reaches more than 20%. Biofilm, as one of the virulence factors of MRSA, can result in the failure of antibiotic therapy. According to reports, bacteriocins, such as peptides synthesized by Gram-negative and Gram-positive bacteria, have antimicrobial activity that has the potential to inhibit antibiotic-resistant pathogens and biofilms formed by MRSA. Result: The bacteriostatic and bactericidal activity of bacteriocins against MRSA has been shown through research across several countries on the usage of bacteriocins, which was isolated from different types of bacteria against MRSA biofilms. Bacteriocins contribute to the inhibition of MRSA biofilms by inhibiting the synthesis of cell walls, leading to pores in the cytoplasmic membranes of bacterial cells, interrupting the synthesis of extracellular membranes, disrupting cell membranes, and reducing the number of planktonic cells within MRSA biofilms. Conclusion: Bacteriocins have an effective mechanism for treating MRSA biofilms with low toxicity and risk of resistance, hence they are safe to be developed as complementary components to antibiotics in an effort to treat MRSA biofilms.
Detection Of Bordetella Pertussis the Cause of Whooping Cough: A Narrative Review Adiyaksa, Jongga; Tjampakasari, Conny Riana
Biomedika Vol 16, No 1 (2024): Biomedika Februari 2024
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v16i1.2799

Abstract

Bordetella Perussis transmits pertussis disease, known as whooping cough, through airborne secretions from the respiratory tract. The bacteria then attach to the epithelial cilia of the respiratory tract of other hosts. One of the main virulence factors produced by Bordetella pertussis is pertussis toxin (PTx) which in its inactivated form is the main component of all acellular pertussis vaccines. In addition, PTx also activates several receptors and can affect various signaling pathways independent of ADP ribosylation and adenylate cyclase. PTx has been used in many research fields due to its strong ADP-ribosylation properties. To detect PTx Bordetella pertussis can use diagnostic tests such as serology test. Aim of this literary study is to determine the types of examinations for the detection of Bordetella pertussis. This study used a narrative review method sourced from searches on PubMed and ScienceDirect. The results of this search showed various types of Bordetella pertussis detection, including detection by culture, serology, and PCR. In conclusion, culture is still recommended to assess vaccine efficacy and future vaccine development, serological tests are one of the main diagnostic procedures in cases of unvaccinated adolescents and adults using specific B. pertussis proteins as antigens, in the presence of increased IgA or IgG titers against the toxin pertussis (PT) and filamentous hemmagglutinin (FHA). PCR as a diagnostic tool for pertussis in neonates and children.
Bakteri Gram positif Listeria monocytogenes sebagai Penyebab Food-borne Disease Conny Riana Tjampakasari
Cermin Dunia Kedokteran Vol 48 No 1 (2021): Infeksi COVID-19
Publisher : PT Kalbe Farma Tbk.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55175/cdk.v48i1.29

Abstract

Bakteri Listeria monocytogenes (L. monocytogenes) merupakan salah satu patogen food-borne penyebab listeriosis dengan prevalensi rendah, namun dengan fatality tinggi sebagai pencemar makanan. Listeriosis merupakan penyakit infeksius yang menyebabkan aborsi, meningitis, dan septikemia; cenderung lebih fatal pada kelompok populasi ibu hamil, bayi, lanjut usia, serta penderita imunodefisiensi. Meskipun kasus infeksi bakteri ini jarang, namun klinisnya termasuk parah dengan angka kematian tinggi. Deteksi laboratorium untuk diagnosis dapat dengan metode analytic konvensional dan metode cepat. Upaya pengendalian infeksi bakteri ini adalah dengan pencegahan dan pengobatan infeksi. Listeria monocytogenes (L. monocytogenes) bacteria is a food-borne pathogen with a low prevalence rate but with a high fatality rate as a food contaminant. Listeriosis is an infectious disease that causes abortion, meningitis and septicemis; tends to be more fatal in population of pregnant women, infants, the elderlies, and immunodeficiency sufferers. Although cases are rare, the clinical conditions are categorized as severe with a high mortality rate. Laboratory diagnosis can be done with conventional analytic and rapid methods. Infections can be controlled with prevention and treatment.