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Bakteri Anaerob Clostridium botulinum dan Toksin yang Dihasilkannya Conny Riana Tjampakasari; Rifdah Hanifah
Cermin Dunia Kedokteran Vol 49 No 5 (2022): Neuro-Kardiovaskular
Publisher : PT Kalbe Farma Tbk.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55175/cdk.v49i5.230

Abstract

C. botulinum adalah salah satu bakteri paling patogen karena dapat menghasilkan botulinum neurotoxin (BoNT) yang mematikan. Terdapat 3 jenis botulisme, yaitu botulisme keracunan makanan, botulisme inhalasi, dan botulisme luka. Meskipun kejadian botulisme jarang, namun harus diwaspadai karena cukup fatal. Sebagian besar kasus botulisme pada manusia disebabkan oleh makanan kaleng yang dipersiapkan di rumah. Isolasi dan identifikasi C. botulinum dapat dilakukan dengan pemeriksaan pewarnaan Gram, kultur, dan identifikasi, sedangkan deteksi toksin dapat menggunakan metode mouse lethality assay, non-lethal mouse assay, dan metode imunologi. Pendekatan molekuler dilakukan melalui uji polymerase chain reaction (PCR) untuk deteksi jenis toksin. Pencegahan botulisme dilakukan dengan teknik penanganan makanan yang tepat. Pemanasan yang memadai dapat membunuh spora bakteri, selain itu segera mengonsumsi makanan yang telah dimasak dapat mencegah C. botulinum bertumbuh. C. botulinum is one of the most pathogenic bacteria because it can produce deadly botulinum neutotoxin (BoNT). There are 3 types of botulism: botulism poisoning, inhalation botulism, and wound botulism. Although the incidence of botulism is rare, the impact is quite fatal. Most cases of botulism in human are caused by canned food prepared at home. Isolation and identification of C. botulinum can be done by Gram staining, culture, and identification, while the detection of toxins can use the mouse lethality assay, non-lethal mouse assay, and immunological methods. Molecular approach can be done through polymerase chain reaction (PCR) examination to detect the toxin type. Prevention can be applied with proper food handling techniques. Adequate heating can kill bacterial spores, and direct consumption can prevent proliferation of C. botulinum.
Infeksi Jamur Oportunistik Pneumocystis jirovecii Conny Riana Tjampakasari
Cermin Dunia Kedokteran Vol 45 No 12 (2018): Interna
Publisher : PT Kalbe Farma Tbk.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55175/cdk.v45i12.684

Abstract

Pneumocystis jirovecii (P. jirovecii) sebelumnya dikenal sebagai Pneumocystis carinii f. sp. Hominis, yang diketahui sebagai penyebab infeksi oportunistik saluran pernapasan bawah pada individu dengan sistem kekebalan tubuh lemah, terutama penderita infeksi HIV. PjP terdistribusi di seluruh dunia dan dapat menyerang berbagai usia. P. jirovecii mempunyai beberapa faktor virulensi antara lain major surface glycoprotein (MSG), DHPS (dihidropteroat sintase), dan mtLSU (mitochondrial large subunit). Diagnosis PjP mengandalkan pemeriksaan mikroskopis dari berbagai spesimen saluran pernapasan. Metode lain adalah diagnosis molekuler untuk deteksi dan karakterisasi DNA. Pengobatan lini pertama dengan kombinasi trimetoprim-sulfametoksazol (TMP-SMX). Beberapa negara termasuk Indonesia telah melaporkan beberapa genotip resisten terhadap beberapa antibiotik. Penyebab resistensi antibiotik dapat disebabkan oleh pemakaian jangka panjang atau kesalahan deteksi karena seringkali gejalanya tidak khas. Pneumocystis jirovecii (P. jirovecii) previously known as Pneumocystis carinii f. sp. hominis are known to be the cause of opportunistic lower respiratory tract infections in individuals with decreased immunity, especially HIV infection. PjP is distributed throughout the world and can attack various ages. P. jirovecii has several virulence factors including major surface glycoprotein (MSG), DHPS (dihydropteroate synthase), and mtLSU (mitochondrial large subunit). Diagnosis of PjP relied on microscopic examination of various respiratory specimens. Another method is molecular diagnosis for detecting and characterizing DNA. The first-line treatment use trimethoprim-sulfamethoxazole (TMP-SMX). Some countries including Indonesia have reported resistance to some antibiotics. It can be caused by long-term use or diagnostic error because of atypical symptoms.
Patogenesis dan virulensi Burkholderia pseudomallei penyebab melioidosis dan Burkholderia cepacia sebagai patogen oportunis Conny Riana Tjampakasari
Jurnal Biomedika dan Kesehatan Vol 4 No 1 (2021)
Publisher : Fakultas Kedokteran Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/JBiomedKes.2021.v4.27-36

Abstract

Di antara genus Burkholderia terdapat dua spesies yang menjadi perhatian dalam bidang kesehatan, yaitu B. pseudomallei dan  B. cepacia.   Kedua bakteri ini menyebabkan masalah klinis yang berbeda. Penyakit melioidosis kerap disebabkan oleh  B.pseudomallei, sedangkan  B. cepacia complex (Bcc) seringkali ditemukan pada pasien cystic fibrosis (CF). Burkholderia pseudomallei merupakan kelompok bakteri patogen intracellular Gram negatif, memiliki bentuk seperti peniti. Demikian pula  B. cepacia  merupakan kelompok bakteri Gram negatif basil serta mempunyai flagel polar multitrik. B. pseudomallei memiliki kemampuan untuk menginfeksi berbagai jenis sel dan menghindari respon imun manusia. Bakteri  ini masuk melalui kulit atau selaput lendir dan bereplikasi di sel epitel. Di dalam sel inang, bakteri bergerak dengan menginduksi polimerisasi aktin inang,  mendesak dinding membran membentuk tonjolan yang meluas ke sel lain. Tonjolan ini menyebabkan sel tersebut  bergabung, membentuk sel raksasa berinti (multinucleated giant cell/MNGC). Setelah memasuki saluran pernafasan pasien penderita CF, B. cepacia menempel pada permukaan sel mukosa ataupun sel epitel inang.  Lapisan mukus yang menebal pada paru mendukung efikasi antimikrobia dan meningkatkan respon inflamasi. Kemampuan untuk melewati barier epitelial dan menemukan akses ke aliran darah hanya dimiliki oleh strain kelompok ini. Faktor virulensi bertugas  membantu proses invasi sel inang oleh bakteri patogen. Secara umum, kedua spesies ini memiliki jenis faktor virulensi yang sama, diantaranya adalah  intracellular survival, quorum sensing, adherence factor, sistem sekresi, lipopolisakarida (LPS) dan eksopolisakarida (EPS),  biofilm, toksin dan resistensi antimikrobia.
Distribution of blaCTX-M, blaSHV, blaTEM genes in Extended Spectrum β-Laktamase Producing Klebsiella pneumoniae from Clinical Isolates in Jakarta Engel, Yulia; Conny Riana Tjampakasari; Fithriyah Sjatha
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 26 No. 02 (2025): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/eksakta/vol26-iss02/549

Abstract

Klebsiella pneumoniae is one of the ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp.) due to their high level of antibiotic resistance. Ceftriaxone is one of the cephalosporin antibiotic that functions inhibit bacterial cell wall synthesis and used for treating K. pneumoniae infections. Resistance to ceftriaxone in K. pneumoniae has been widely reported, with one contributing factor being the production of β-lactamase enzymes encoded by the genes blaCTX-M, blaSHV, and blaTEM. This study characterized the presence of these genes  in 12 clinical isolates of         K. pneumoniae and analyzed their correlation with phenotypic resistance to ceftriaxone. All isolates characterized with antimicrobial susceptibility testing (AST) and disk diffusion methods to evaluate the phenotypic production of blaCTX-M, blaSHV, and blaTEM. Molecular analysis using the polymerase chain reaction (PCR) method showed the genes blaCTX-M and blaTEM were detected in 11 isolates (91.67%), and blaSHV was found in 9 isolates (75%). The distribution pattern of the blaCTX-M, blaSHV and blaTEM resistance genes was present in 8 isolates (66.67%), with MIC values > 64 µg/mL. The presence of blaCTX-M, blaSHV, and blaTEM genes together in K. pneumoniae isolates represents a potential risk for resistance to other β lactam antibiotics.
Potential of Moringa oleifera (M. oleifera) Leaf Extract as an Antiseptic against Candida albicans using Percentage Kill Test Tjampakasari, Conny Riana; Kiranasari, Aryani; Baswara, R. Muh. Kevin
Majalah Obat Tradisional Vol 30, No 2 (2025)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.100119

Abstract

Candida albicans is a commensal microorganism that is commonly found as a normal flora in the body. However, impaired immune conditions can cause this fungus to turn into a pathogen. This Microorganism is one of the causes of mortality and morbidity in the world. The use of antiseptics is beneficial for the prevention and treatment of fungal infections. Moringa oleifera is a plant that is often found in Asia and Africa, has various bioactive components that have the potential to be antiseptic. Until now, there has been no research on the antiseptic potential of M. oleifera against C. albicans. Therefore the study was conducted to test the activity of Moringa leaf extract (Moringa oleifera) as an antiseptic against C. albicans. This experiment was conducted using the percentage kill method to determine the potential activity of the extract as an antiseptic against C. albicans The comparison of bacterial colony growth on the control and the treatment that grew on solid media was calculated according to the set contact time of 1, 2, and 5 minutes. The effectiveness of antiseptics is assessed by calculating according to the percentage kill principle. The results of the calculation of C. albicans colonies in contact time for 1, 2, and 5 minutes were 62.39%, 80.85%, and 90%, respectively. This finding shows that the contact time of 5 minutes has good effectiveness. Therefore M. oleifera leaves have the potential to be an effective antiseptic against C. albicans.
Sampling of Sars-Cov-2 Covid-19 an Air Using the Impingement Method with Real-Time PCR as Detection Examination Tjampakasari, Conny Riana; Yasmon, Andi; Rosa, Yulia; Sjatha, Fithriyah
East Asian Journal of Multidisciplinary Research Vol. 3 No. 3 (2024): March 2024
Publisher : PT FORMOSA CENDEKIA GLOBAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55927/eajmr.v3i3.8651

Abstract

Examination of airborne microbial for SARS-CoV-2, Coronavirus Disease 2019 (Covid-19) has not been widely developed. Whether this virus is airborne is still an unanswered question. This preliminary study was to evaluate the presence of the Covid-19 in the air. The study was conducted on 4 rooms, which are laboratory rooms used for Covid-19 activities. The impingement which is an active method used for air sampling, while the detection was carried out through a molecular approach using RT real-time  PCR. The overall Covid-19 virus was not found in the room being examined. Four rooms gave CT values above the positive control, that is administration room (CT 30.44), BSL room (CT 30.23), BSC (CT 30.69 ) and waiting room (CT 35.61). No found SARS-CoV-2 Covid-19 in air using the impingement method with real-time PCR as detection examination in the room being examination.  Preliminary study shown SARS-CoV-2 Covid-19 can not transmitted through the air. Research needs to be done with more sampling as continuation of this study.
Clostridium difficile Problematik Infeksi Clostridium difficile: Infeksi C. difficile Tjampakasari, Conny Riana; Hanayurianingtyas, Deajeng Laras
Jurnal Biomedika dan Kesehatan Vol 6 No 2 (2023)
Publisher : Fakultas Kedokteran Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/JBiomedKes.2023.v6.235-249

Abstract

Clostridium difficile is a Gram-positive, strictly anaerobic, spore-forming bacterium. The virulence factor of this bacterium is the toxins it produces, namely enterotoxin A (TcdA ) and cytotoxin B (TcdB). Transmission occurs fecally orally by ingesting the spores of this bacteria. Clostridium difficile is the most common cause of fulminant-associated in hospitals and other healthcare facilities and is of significant concern because of the increasing morbidity and mortality rates as well as increased healthcare costs. Elderly patients or patients with compromised immune systems are patients at the highest risk for this case. Clostridiosis present a varied spectrum of infection, ranging from self-limited diarrhea, mild to moderate diarrhea, to serious diarrhea, pseudomembranous colitis, and life-threatening fulminant colitis which can be life-threatening and can cause death. The diagnosis is made by direct detection of Clostridium difficile toxin in the feces. Although empiric therapy known as vancomycin and fidaxomycin have been used as the antibiotic choice, prompt identification of patients with symptoms of Clostridium difficile infection is very important because most patients respond quickly to antimicrobial therapy. may result in death. Prompt identification of patients with symptomatic Clostridium difficile infection is essential as the majority of patients respond quickly to antimicrobial therapy. Prevention is best achieved by implementing infection control measures and appropriate use of antimicrobial agents. Health professional education regarding preventive measures such as hand washing, wearing gloves, decontamination of medical equipment, and the proper environment is highly recommended.
Kultivasi dan Identifikasi Bakteri Anaerob Bacteroides Fragilis Tjampakasari, Conny Riana; Hanifah, Nadyatul
MAHESA : Malahayati Health Student Journal Vol 3, No 11 (2023): Volume 3 Nomor 11 (2023)
Publisher : Universitas Malahayati

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/mahesa.v3i11.11537

Abstract

ABSTRACT Anaerobic infections are infections caused by bacteria that grow and develop without the need for oxygen. Anaerobic bacteria are found in various human bodies such as on the skin, mucosal surfaces, and are in high concentrations in the mouth and digestive tract as part of the normal flora. These bacteria can infect deep wound, deeper tissues, and internal organs thet require ilttle oxygen. Cultivation and identification of anaerobic bacteria is one the most important steps as the basis diagnosis of a disease. Cultivation can be done by choosing the right medium, while to get a growth environtment without oxygen, an anerobic jar equipped with an anaerogen chemical gas generation sachet is used. The process of identifying bacteria was carried out starting from Gram staining follwed by a carbohydrate utilitization examination. Technological developments support the development of various automatic methods to identify anaerobic bacteria, one of which is the Vitek-2 machine. Bacteroides fragilis from clinical specimens was successfully identified with a probability of >90%, Gram negative, rod-shaped anaerobic bacterium. Keywords: Cultivation, Identification, Anaerobic Bacteria  ABSTRAK Infeksi anaerob adalah infeksi yang disebabkan oleh bakteri yang tumbuh dan berkembang tanpa membutuhkan oksigen. Bakteri anaerob ditemukan pada berbagai tubuh manusia seperti di kulit, permukaan mukosa, dan berada dalam konsentrasi tinggi di mulut dan saluran pencernaan sebagai bagian dari flora normal. Bakteri ini dapat menginfeksi luka yang dalam, jaringan yang lebih dalam, dan organ dalam yang membutuhkan sedikit oksigen. Kultivasi dan proses identifikasi bakteri anaerob menjadi salah satu tahapan yang sangat penting sebagai dasar diagnosis terhadap suatu penyakit. Kultivasi dapat dilakukan dengan pemilihan medium yang tepat sedangkan untuk mendapatkan lingkungan pertumbuhan tanpa oksigen digunakan jar anaerob yang dilengkapi dengan gas generation sachet anaerogen  chemical. Proses identifikasi bakteri dilakukan mulai dari pewarnaan Gram dilanjutkan dengan uji pemanfaatan karbohidrat. Perkembangan teknologi menunjang berkembangnya berbagai metode otomatis untuk melakukan identifikasi bakteri anaerob, salah satunya adalah  mesin Vitek-2. Bacteroides fragilis dari spesimen klinik berhasil diidentifikasi dengan probabilitas >90%, merupakan bakteri anaerob berbentuk batang bersifat Gram negatif. Kata Kunci: Kultivasi, Identifikasi, Bakteri Anaerob
Profil Genotipik Extraintestinal pathogenic Escherichia coli (ExPEC) Penyebab Bloodstream Infection Menggunakan Whole Genome Sequencing: Literatur Review Stevina, Lany; Tjampakasari, Conny Riana; Paramita, Rafika Indah
Syntax Literate Jurnal Ilmiah Indonesia
Publisher : Syntax Corporation

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36418/syntax-literate.v9i7.15845

Abstract

Penelitian ini bertujuan untuk menganalisa profil fenotipik dan genotipik pola kepekaan antibiotik pada Escherichia coli (E.coli) penyebab Bloodstream infection (BSI). Metode penelitian yang digunakan adalah literature review. Pencarian literatur menggunakan data dari publikasi ilmiah mulai dari tahun 2020 hingga tahun 2024 dengan menggunakan beberapa pencarian database seperti ScienceDirect (https://www.sciencedirect.com); PubMed (https://pubmed.ncbi.nml.nih.gov); dan Google Scholar (https://www. scholar.google.com). Berdasarkkan kata kunci dan kriteria inklusi yang digunakan, total ditemukan 236 artikel. Setelah dilakukan penyaringan didapatkan empat artikel yang ditinjau dan ditelaah lebih lanjut berdasarkan kecocokan judul dan abstrak. Berdasarkan hasil review jurnal, penelitian menyimpulkan bahwa karakterisasi secara fenotipik dan genotipik tidak hanya akan meningkatkan ketepatan diagnostik bakteri penyebab BSI tetapi juga dapat memberikan pemahaman mendalam mengenai sifat biologis dan epidemiologi penyakit. Sehingga, tidak hanya mendukung pengambilan keputusan klinis yang lebih akurat melainkan juga memungkinkan upaya pencegahan serta pengendalian infeksi.