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All Journal Jurnal Teknik ITS Jurnal Sains dan Seni ITS IPTEK Journal of Proceedings Series IPTEK The Journal for Technology and Science Bioeksperimen: Jurnal Penelitian Biologi Jurnal Biodjati Akta Kimia Indonesia Jurnal Purifikasi Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Sewagati IJOBI
Enny Zulaika
Jurusan Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Institut Teknologi Sepuluh Nopember (ITS)
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Neuroscience Public Health Social Sciences Other

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Pemanfaatan Greenhouse sebagai Wahana Edukasi di Desa Kebontunggul Kecamatan Gondang Kabupaten Mojokerto Tutik Nurhidayati; Maya Shovitri; Nengah Dwianita Kuswytasari; Enny Zulaika; Nur Hidayatul Alami; Arif Luqman; Dini Ermavitalini; Kristanti Indah Purwani; Wirdatul Muslihatin; Imam Wahyudi Farid; Ciptian Weried Priananda; Sri Fatmawati
Sewagati Vol 7 No 6 (2023)
Publisher : Pusat Publikasi ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j26139960.v7i6.515

Abstract

Salah satu desa yang memiliki potensi untuk dikembangkan sebagai desa wisata adalah desa Kebontunggul Kecamatan Gondang Kabupaten Mojokerto. Desa tersebut memiliki banyak potensi desa wisata yang strategis. Disisi lain ada keinginan untuk melakukan kegiatan pertanian berbasis teknologi dalam skala greenhouse untuk mendukung usaha wisata di Desa Kebontunggul. Adanya potensi dan keingginan untuk maju tersebut maka langkah pertama yang dilakukan dalam pengabdian masyarakat adalah pembuatan greenhouse sebagai sarana untuk pengembangan teknologi IoT. Selain itu adanya greenhouse di desa menjadi salah satu pengembangan desa dengan ikon terbaru berupa wisata edukasi. Hasil survei kebermanfaatan greenhouse menunjukkan bahwa greenhouse bermanfaat bagi masyarakat Kebontunggul untuk wahana wisata yang menarik banyak wisatawan.
Analysis of Bacterial Diversity In Temple Bricks Using Phenetic Numeric Taxonomy Method Alifia, Luluk; Zulaika, Enny; Soeprijanto, Soeprijanto
Jurnal Biodjati Vol 9, No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.33476

Abstract

Temple bricks-based constructions are known to often face various challenges, including physical, chemical, and biological weathering. Previous studies identified various biological factors contributing to brick weathering including exudate produced by microorganisms, such as bacteria. In addition, bacteria often live synergistically and antagonistically with other species, exhibiting diverse morphological and physiological traits (bacterial diversity). Various methods have been developed to explore bacterial diversity, with phenetic numerical taxonomy being the most popular. Therefore, this study aims to determine bacterial diversity on the surfaces of temple bricks using phenetic numerical taxonomy method. Bacterial isolation was carried out aseptically, followed by labeling and transferring the isolate to the laboratory for further tests. The tests carried out included morphological characterization, biochemical assays, physiological reactions, and potential enzymatic activities. Subsequently, dendrogram was constructed using MVSP (Multi-Variate Statistical Package) software with isolated grouping based on the Unweighted Pair Group Method Averages (UPGMA) algorithm. The similarity between isolates was analyzed using the Simple Matching Coefficient (SSM) similarity value. The dendrogram analysis revealed the presence of 3 clusters namely A (4 isolates), B (1 isolate), and C (2 isolates), with a similarity index of 0,543 to 0,857. Clusters A, B, and C had a similarity index of ≤0.700, indicating the occurrence of distinct species in each cluster. Based on the profile-matching results of key characters, the 7 bacterial isolates were identified as belonging to the genera Bacillus, Corynebacterium, and Mycobacterium.
PHENETIC DIVERSITY OF CELLULASE-PRODUCING BACTERIA FROM WANA TIRTA KULON PROGO MANGROVE FOREST Febriani, Heni; Octavia, Bernadeta; Zulaika, Enny
Indonesian Journal of Bioscience (IJOBI) Vol. 1 No. 1 (2023): Indonesian Journal of Bioscience (IJOBI)
Publisher : Universitas Negeri Yogyakarta in collaboration with Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/ijobi.v1i1.114

Abstract

Mangrove is an ecosystem that have important value for the environment and are a source of cellulase-producing microorganism biomass. The purpose of this study was to determine the characteristics and types, as well as the highest cellulase enzyme activity from cellulase-producing bacteria found in Wana Tirta Mangrove Forest, Jangkaran, Kulon Progo. This research is a descriptive-exploratory research. Sampling in the form of litter, mud and water was carried out in the Wana Tirta Kulon Mangrove Forest which was divided into 3 plots. This sample is then isolated on selective media in the form of Carboxymethyl cellulose (CMC) In order to grow cellulolytic bacteria. The bacteria obtained are then purified and phenetic characterization. The data obtained were used to classify the bacterial isolates using MVSP identification software (Multivariate Statistical Package) 3.1 with UPGMA clustering algorithm (Unweight Pair Group Method With Arithmatic Averages) then the result is presented in the form of a dendogram. Result Research shows as many as 17 isolates of cellulolytic bacteria were obtained. There were 4 bacterial isolates from litter samples, 1 bacterial isolate from water samples and 12 bacterial isolates from mud samples. After being made in the form of a dendogram, 19 bacterial clusters were obtained. A total of 6 bacterial isolates had a similarity index of ≥ 72% against Bacillus pumilus namely isolates S1A 2, S2A, AL 3, AP 3, AP 6 and AP 17, 4 bacterial isolates have a similarity index of ≥ 70% to Bacillus stearothermophyllus namely AP 22, AP 24, AP 25, and AP 26 isolates. As well as 7 bacterial isolates have a similarity index of ≥ 77% against Streptomyces Sp. namely isolates S1A 1, S3B, AP 8, AP 9, AP 14, AP 20 and AP 27.The highest cellulase enzyme activity occurred in AP 14 bacterial isolate of 286.72 U / ml.
Analysis of Bacterial Diversity In Temple Bricks Using Phenetic Numeric Taxonomy Method Alifia, Luluk; Zulaika, Enny; Soeprijanto, Soeprijanto
Jurnal Biodjati Vol 9 No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.33476

Abstract

Temple bricks-based constructions are known to often face various challenges, including physical, chemical, and biological weathering. Previous studies identified various biological factors contributing to brick weathering including exudate produced by microorganisms, such as bacteria. In addition, bacteria often live synergistically and antagonistically with other species, exhibiting diverse morphological and physiological traits (bacterial diversity). Various methods have been developed to explore bacterial diversity, with phenetic numerical taxonomy being the most popular. Therefore, this study aims to determine bacterial diversity on the surfaces of temple bricks using phenetic numerical taxonomy method. Bacterial isolation was carried out aseptically, followed by labeling and transferring the isolate to the laboratory for further tests. The tests carried out included morphological characterization, biochemical assays, physiological reactions, and potential enzymatic activities. Subsequently, dendrogram was constructed using MVSP (Multi-Variate Statistical Package) software with isolated grouping based on the Unweighted Pair Group Method Averages (UPGMA) algorithm. The similarity between isolates was analyzed using the Simple Matching Coefficient (SSM) similarity value. The dendrogram analysis revealed the presence of 3 clusters namely A (4 isolates), B (1 isolate), and C (2 isolates), with a similarity index of 0,543 to 0,857. Clusters A, B, and C had a similarity index of ≤0.700, indicating the occurrence of distinct species in each cluster. Based on the profile-matching results of key characters, the 7 bacterial isolates were identified as belonging to the genera Bacillus, Corynebacterium, and Mycobacterium.
POTENSI Azotobacter spp. SEBAGAI PENDEGRADASI LIPID DAN PROTEIN Firdausi, Waritsatul; Zulaika, Enny
Bioeksperimen: Jurnal Penelitian Biologi Vol 1, No 2: September 2015
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v1i2.873

Abstract

Azotobacter spp. merupakan salah satu genus bakteri nonsimbiotik yang berperan penting dalam bidang agrikultur terkait kemampuannya memfiksasi nitrogen. Penelitian ini bertujuan untuk mengetahui potensi isolat Azotobacter dari lahan Eco Urban Farming ITS dalam mendegradasi lipid dan protein. Kemampuan isolat dalam mendegradasi lipid dan protein ditentukan dengan pengukuran Indeks Biodegradasi (IB). Isolat yang digunakan adalah A1a, A1b, A2, A3, A5, A6, A7, A8, A9, dan A10. Semua isolat mampu mendegradasi lipid kecuali A5, dengan IB tertinggi pada isolat A1b (1,00). Semua isolat dapat mendegradasi protein kecuali A7, dengan IB tertinggi terdapat pada isolat A8 (3,00).
Co-Authors Adi Soeprijanto Adisya Prima Nurmalita Sari Afianita Dian Rahmawati Afriha Mafazah Alifia, Luluk Anies Wijayanti Anindya Citra Asri Anjar Lulu Sakinah Apriliani Devinta Arif Luqman Arzulinda Maulidar Rahmasyitha Aunurohim Aunurohim Avip Nur Fitria Ciptian Weried Priananda Denaya Andrya Prasidya Dewi Hidayati Dini Ermavitalini Djoko Purwanto Edwin Setiawan Fahima Tahta Kholida Farid Kamal Muzaki Febriana Puji Rahayu Gian Rukmana Heni Febriani, Heni Hermawan Hilda El Yasa Alam Idea Rahmania Machsun Imam Wahyudi Farid Indah Budi Asih Iska Desmawati Kristanti Indah Purwani Kusnul Khotimah Kuswytasari Kuswytasari Kuswytasari, Nengah Dwianita Laily Hanifa Lericka Mei Permadi Lilik Nurindah Sari N. D Kuswytasari Nadia Ulfiyati Nitra Wahyuningsih Nova Maulidina Ashuri Nur Hidayatul Alami Nurul Jadid Octavia, Bernadeta Prajna Wirya Kencana Putra Rachma Shouma Annisa Rahma Nadia Maudy Rindan Novanti Riska Irawan Rosidah Kumalasari Ryan Widi Anggar Kusuma Salma Shavira Rahma Khofifah Saptarini, Dian Septa Tri Farisna Shovitri, Maya Sri Fatmawati Tania Pratiwi Trio Verdian Tutik Nurhidayati Wahyu Dewi Iftita Waritsatul Firdausi Waritsatul Firdausi Waritsatul Firdausi Windasari Putri Septarina Wirdatul Muslihatin