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All Journal Jurnal Ilmu Ternak Indonesian Journal of Biotechnology JURNAL ILMIAH PETERNAKAN TERPADU JURNAL KAJIAN VETERINER
Victor Lenda
Program Studi Kesehatan Hewan Jurusan Peternakan Politeknik Pertanian Negeri Kupang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary Other

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Identifikasi dan Karakteristik Staphylococcus Sp. dan Streptococcus Sp. dari Infeksi Ovarium Pada Ayam Petelur Komersial (Identification and Characteristics of Staphylococcus Sp. and Streptococcus Sp. Infection of Ovary in Commercial Layers) Novianti Neliyani Toelle
Jurnal Ilmu Ternak Vol 14, No 1 (2014)
Publisher : Fakultas Peternakan, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jit.v14i1.5145

Abstract

Infeksi ovarium merupakan salah satu penyakit yang paling sering ditemukan pada ayam petelur menjelang periode  betelur  maupun  selama  masa  produksi.  Gangguan  produksi  telur  dapat  terjadi  karena  gangguanperkembangan ovarium akibat gangguan pertumbuhan pada ayam, kerusakan ovarium atau akibat penyakit. Penelitian ini dirancang untuk mengisolasi, mengidentifikasi, dan karakteristik bakteri penyebab infeksi ovarium pada ayam petelur komersial. Sejumlah 19 sampel ayam petelur komersial yang diperoleh dari tiga peternakan di wilayah  Yogyakarta.  Ovarium  yang  mengalami  infeksi  dikultur  pada  plat  agar  darah  (PAD),  kemudian diinkubasi pada suhu 37°C selama 18-24 jam. Setelah pengelompokkan koloni dilanjutkan pengecatan Gram. Gram positif bentuk kokus dilakukan uji katalase. Selanjutnya bakteri dengan katalase positif dilakukan uji koagulase, kultur pada mannitol salt agar (MSA), Voges Proskauer (VP) dan uji oksidase. Hasil identifikasi dan karakteristik bakteri dari infeksi ovarium adalah Staphylococcus aureus (46%), Staphylococcus intermedius (27%), Staphylococcus epidermidis (20%), dan Streptococcus sp. (7%).Kata kunci : Ovarium, ayam petelur, Staphylococcus sp., Streptococcus sp.
Histological Features of Catecholaminergic Neuron in Substantia Nigra Induced by Paraquat Dichloride (1,1-dimethyl-4,4 bipyridinium) in Wistar Rat as A Model of Parkinson Disease Tri Wahyu Pangestiningsih; Woro Danur Wendo; Yulfia Nelymalik Selan; Filphin Adolfin Amalo; Nemay Anggadewi Ndaong; Victor Lenda
Indonesian Journal of Biotechnology Vol 19, No 1 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (425.756 KB) | DOI: 10.22146/ijbiotech.8638

Abstract

Paraquat dichloride has been used by farmers as a herbicide to kill the grass. On the other hand, paraquatdichloride is harmful if enters to the body, causing Parkinson’s disease, since it is disrupting dopamineproduction in the substantia nigra pars compacta or dopamine pathways Nigro striatal pathway. The studywas done to fi nd out the histological changes of catecholaminergic neurons and Nigro striatal pathway causedby paraquat dichloride treatment in Wistar rats as a model of Parkinson’s disease.Twenty-two Wistar rats 3,5 months old were divided into 4 groups, 5 rats each. Group I (control group)were injected with aquabidest, while groups II, III, and IV were injected intraperitoneally with paraquatdichloride in aquabidest, with the dosage 5 , 10 and 15 mg/kg bw respectively. The rats were injected onceper week for 6 weeks. Three days after the last injection, the rats were anesthetized using xylasin (2 mg/kg)and ketamine (20 mg/kg) intramuscularly, and then were intracardiac perfused using physiological saline asprerinse solution, followed by 10% buffered formalin solution as a fi xative. After animals were fi xed, the brainswere removed and embedded in paraffi n block and cut in 12 μm thickness for immunohistochemistry stainingusing tyrosine hydroxylase antibody. The results of staining then were observed under light microscope andanalyzed descriptively.The results showed that the catecholaminergic neurons were distributed in the substantia nigrapars compacta in all treatment groups, however, the cell density were found decreased only in group IV.Catecholaminergic neurons appear in the bipolar and multipolar form, while dopamine ‘Nigro striatal pathway’was found exist in all treatment groups. From our study, histologycally the decreased of catecholaminergicneurons is only found in rats that received paraquat dichloride in dose 15 mg/kg bw for 6 weeks.
Karakteristik Pasteurella multocida Penyebab Pasteurellosis pada Babi di Kota Kupang Provinsi Nusa Tengggara Timur Victor Lenda; Filphin A. Amalo; Yulfia N. Selan
JURNAL KAJIAN VETERINER Vol 6 No 2 (2018): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v6i2.458

Abstract

The number of commercial pigs in East Nusa Tenggara has grown fast with a population of 1,739,481, and has become more potential. However, the mixed farming model has become one of the factor of potentially high in the transmission of disease-causing pathogenic microorganisms. One of the microorganism is Pasteurella multocida which causes pasteurellosis, has been identified in 25% of slaughtered pigs (Maes et al., 2001). One of the clinical symptom due to pasteurellosis in pigs is the occurrence of bronchopneumonia in pulmo and inflammation in various visceral organs, such as the heart and kidneys. the phenotypic characteristization of this bacteria, will be very helpful in designing a comprehensive prevention and treatment programs of pig pasteurellosis. The aim of the research was to determine the characteristics of P. multocida related to pasteurellosis and recording of the disease in Kupang, NTT. This research also find out the phenotipyc characteristics of P. multocida species from pigs and the possibility of transmission among sensitive species. A total of 30 swine lung samples of pulmo were obtained from slaughterhouse in Kupang to carry out this study. Pulmo taken from slaughtered pigs that showed clinical respiratoric symptoms such as dyspnoea and the presence of serous to mucopurulent nasal exudates, and the specific lesions of gray hepatization in pulmo. The collected samples were then processed for histopathological and microbiological studies. Out of the total 30 sample, 15 samples were found to be suspected for pasteurellosis, and 3 samples were successfully confirmed to be positive for Pasteurella multocida. Varied macroscopic changes showed pathognomonic lesions as multifocal hemorrhage and congestion of the pulmonary lobes. Serous to mucopurulent exudate were found in lumen bronchus. Multi lobes grayed hepatization and multifocal hemorrhage were observed in the pulmo. Histopatologic analysis showed three types of pneumonia that were multifocal suppurative bronchopneumonia with neutrophil infiltration into alveoli and bronchioles; non-suppurative pneumonia as fibrinous bronchopneumonia with severe congestion, and chronic bronchiolitis with infiltration of mononuclear cell and thickening of fibrous tissue on bronchioles. Bacterial culture from the samples showed circular, convex and non hemolytic colony on blood agar base. Gram staining’s showed Gram negative microorganism with coccoid bipolar structure, which are some of the characteristic of the microorganism.. It was concluded that the samples is having P. multocida infection. Although, some isolate on MacConkey showed lactose fermentation and tolerance to bile salts that were not the nature of the microorganism, isolation and identification from other organs needed to be done, for example from the heart and kidneys, are needed.
GAMBARAN HISTOPATOLOGI BEBERAPA TIPE BRONCHOPNEUMONIA PADA PULMO BABI HASIL PEMOTONGAN DI RPH OEBA Hermilinda Parera; Aven B Oematan; Victor Lenda
JURNAL KAJIAN VETERINER Vol 5 No 2 (2017): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v5i2.951

Abstract

The aim of this study was to determine the histopathologic changes of bronchopneumonia type in pigs of slaughtered pig from Oeba slaughterhouse associated with P. multocida infection. Samples of pulmo were found from pigs showing respiratoric symptoms, characterized as dyspnoea and presence of nasal exudates that are serous to mucopurulent, obtained from slaughtered pigs in RPH Oeba Kota Kupang. Samples showed pathognomonic lesions of gray hepatized than processed for histopathological studies. The result showed that varied macroscopic changes noted in pulmo samples from suspected infected pigs showed various types of lesions. Lessions observed then classified into two main lessions were acute and chronic. Acute lesions were characterized by edema and hyperemia which then confirmed with congestion and multifocal haemorrhages. Serous to seromucous exudates were observed intrabronchial and bronchioles. In chronic cases, the lesions showed cranioventral consolidation and gray hepatized with demarcated area beetwen consolidated and normal pulmonary area. Histologically, the types of bronchopneumonia categorized into bronchopneumonia suppurativa characterized by coagulative necrosis surrounded by cell infiltration and accumulation of fibrin intraalveolar. The fibrous connective tissue were also observed in the interstitaial space of alveolar around the necrotic area. Other samples showed non-suppurativa fibrinous bronchopneumonia with alveolar exudates dominated by mononuclear cells and accumulation of fibrin intra alveolar. Chronic bronchiolitis also seen with a mixture of mononuclear cells, neutrophils and macrophages that infiltrated into the lumen of the alveoli. A high number of goblet cells were observed in the bronchiolus epithelium, with the thickening of the fibrous tissue around the bronchioles. It can be concluded that there are two major types of lesions which are acute and chronic, with three types of bronchopnemonia namely bronchopneumonia suppurativa, non supuratival bronchopneumonia and chronic bronchiolitis.
Fenotipe Pasteurella multocida Penyebab Bronchopneumonia Pada Babi Di Yogyakarta Victor Lenda; Novianti Neliyani Toelle
JURNAL KAJIAN VETERINER Vol 2 No 2 (2014): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v2i2.1000

Abstract

Pasteurella multocida is an important species that are suspected to cause bronchopneumonia in pigs. Prevalence of pneumonia pasteurellosis in slaughter house between 30-80%. The purpose of this study were determined the microorganisms that caused respiratory disorders (cough) or pneumonia in pigs in Yogyakarta area with morphological and biochemical tests. A number of 6 pigs were obtained from some pig farming in Yogyakarta with clinical symptoms of pneumonia were necropted, isolated and identified the cause of pneumonia, followed by histopathological examination of the isolates.The results showed that P. multocida is one of the main causes of bronchopneumonia in pigs in Yogyakarta with clinical symptoms of anorexia, dipsnoea, cough with serous to mucopurulent exudation. Pulmo showed grayish hepatization on dorsocranial, multifocal hemorrhagic and congestion. Histopathological changes found fibrinous bronchopneumonia and catharrhal bronchopneumonia.
Identifikasi Molekuler Pasteurella multocida Penyebab Pasteurellosis Pada Babi Di Yogyakarta Victor Lenda; Hermilinda Parera
JURNAL KAJIAN VETERINER Vol 3 No 1 (2015): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v3i1.1027

Abstract

The purpose of this study was to determine the genetic variation of P.multocida in Yogyakarta with other overseas P. multocida strains. Samples were obtained from necropted intensive pig farming with clinically pneumonia symptoms, specific pulmonary lessions and then followed by histopathological examination and b iochemical characterization of the isolates. Then followed by histopathological examination and biochemical characterization of the isolates. Moleculartest performed by DNA extraction using the QIAGEN QIAamp DNA minikit, amplification of the 16S rRNA gene using forward primer 5' GGA GTG AAC TGC AGC TAA TAC C 3', and reverse primer 5' GTA GGT AAG CTT CGC GTT GTT G 3', electrophoresis, purification and sequencing. Macroscopic and histopathological examination results were analyzed descriptively. Sequencing r esults were analyzed by multiple alignment with other Pasteurella spp. taken from GenBank using the Clustal W software, subsequently analyzed using Neighbour Joining and Maximum Parsimony method that exist in program MEGA version 5.1 The results showed gen etic distance based on 765 nucleotides of 16S rRNA gene of P. multocida isolates from lungs of bronchopneumonia of swine in Yogyakarta at 0%. Filogram based on the nucleotide sequence showed a high similarity between P. multocida isolates from Yogyakarta a nd other isolates from USA, Germany, China, Europe, and Hungaria.
Pengaruh Corpus Luteum Dan Folikel Dominan Terhadap Kualitas Morfologi Oosit Sapi Bali-Timor Hermilinda Parera; Victor Lenda
JURNAL KAJIAN VETERINER Vol 3 No 1 (2015): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v3i1.1031

Abstract

Improvement of cattle production can be obtained by the application of feed and breeding technology. One of the breeding technologies that are rapidly growing is in vitro fertilization, which is part of In vitro embryo production The source of oocytes influence the success of in vitro embryo production The quality of the oocyte were determined by Ovarian follicular environment and followed the follicular growth that were characterized by the presence of a dominant follicle (DF) and corpus lutem (CL). The purpose of this study was to determine the influe nced of dominant follicles and corpus luteum on oocyte morphology ofBali Timor cattle, that were collected by aspiration method. Oocytes were obtained postmortem from the ovaries of Bali Timor cows slaughtered at an Oeba slaughterhouse Ovarian then class ified into 3 groups : I ) ovarian with CL without DF; (II) ovarian with DF, without CL; and (III) ovarian without both DF and CL. The results showed that the number of oocytes with good quality from group III (12.5%) was higher than group I (9.83%), but no significant differently (P> 0.05). The number of oocytes from group II that showed several layers of cells and has a cytoplasm (good quality) was very low.
PENGARUH EKSTRAK DAUN C.ODORATA TERHADAP PROSES KESEMBUHAN LUKA INSISI PADA TIKUS SPRAGUE-DAWLEY Ni Sri Yuliani; Victor Lenda
JURNAL KAJIAN VETERINER Vol 3 No 2 (2015): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v3i2.1034

Abstract

Chromolaena odorata (kirinyu/sufmuti) is a species of weed which utilized as traditional medicine especially for wound healing. This research was conducted to study the histological effect of C.odorata leaf extract to healing process of incision wound in Sprague-Dawley rats. A total of 45 males - 6 weeks of Sprague Dawley rats at 150-200g body weight were utilized in this study. Those rats were divided into five groups, basis ointment, Betadine 10%, C.odorata 5%, C.odorata 10%, and C.odorata 20%. All groups of rats were given a 3 cm long linear incision at dermal depth on the backs. The extract and medication were topically applied once a day for ten days. 3 rats of each group were euthanized in day 2, 6, and 10 after incision. Subsequently, skin tissue was obtained and fixated in 10% buffer formalin for Hematocixylin - Eosin staining. The measured variables were wound healing both macroscopical and microscopical figures from day 0 to day 10 after incision. Data were analyzed descriptively. Microscopic observation showed that various concentrations of C.odorata extracts affected wound healing process. C.odorata Extract is allegedly able to stimulate wound healing by increasing fibroblasts for re-epithelization.
Evaluation of Motility, Viability and Abnormality of Boar Spermatozoa in Various Modified Extenders Hermilinda Parera; Victor Lenda
JURNAL ILMIAH PETERNAKAN TERPADU Vol 11, No 1 (2023)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jipt.v11i1.p13-33

Abstract

The purpose of this study was to conduct an assessment on the quality of pig semen spermatozoa during storage and determine which diluent material should be used and how long pig semen can be stored. Freshly ejaculated boar semen was obtained from a two-years-old Duroc male and was diluted with Beltsville Thawing Solution (BTS) and Tris Citrate Fructose (TCF) supplemented with 5% egg yolk (KT) and extract of Borassus flabellifer Linn mesocarp (EM) 0.01% and stored at 13°C for up to 4 days. An experimental study with a completely randomized design with four groups and five repeats was applied. Parameters observed were motility, viability and abnormality of spermatozoa. Sperm quality was evaluated by examining motility and abnormality using a phase-contrast microscope with a magnification of 100X, and viability by eosin nigrosine staining. Parametric data were analyzed with ANOVA followed by Duncan test.  The results showed that the supplementation of 5% egg yolk in the BTS diluent-based treatment group showed a higher percentage of motility (P<0.05) than without egg yolk, while the TCF-based diluent group showed no differences (P<0.05). Modified-Tris Citrate Fructose diluent supplemented with 5% egg yolk and 0.01% mesocarp extract was able to maintain a significantly higher percentage of viability (67.50±2.0) (P<0.05) than other groups, and had a percentage of abnormalities (9.75±0.957), significantly lower (P<0.05) than other groups. It was concluded that the modification of diluent TCF with 5% egg yolk and mesocarp extract 0.01% provide the best results in maintaining sperm quality with the highest percentage of sperm motility and viability, and the lowest percentage of sperm abnormality compared to the other three diluents. Likewise, all groups of modified-BTS and TCF diluent supplemented with 5% egg yolk and extract of Borassus flabellifer Linn mesocarp in this study were able to maintain motility >40%, viability >45% and abnormalities <20% so they were appropriate for using in the AI procedure.
STUDI KERAGAMAN JENIS DAN POLA AKTIVITAS HARIAN LALAT DI PETERNAKAN SAPI SEMI EKSTENSIF DI KELURAHAN TUATUKA KECAMATAN KUPANG TIMUR KABUPATEN KUPANG Oematan, Aven B.; Sakan, Gerson Y. I.; Moenek, Devi Y. J. A.; Koten, Bernadette B.; Lenda, Victor
JURNAL KAJIAN VETERINER Vol 7 No 2 (2019): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v7i2.1980

Abstract

Tujuan Penelitian ini dilakukan untuk mengetahui keragaman jenis, pola aktivitas lalat dan hubungannya dikaitkan dengan suhu, musim, serta jenis kelamin. Penelitian di peternakan sapi semi ektensif kelurahan tuatuka Kecamatan Kupang Timur, Kabupaten Kupang, Nusa Tenggara Timur. Sampel lalat dikoleksi menggunakan New Zealand 1 trap di peternakan sapi. Perangkap dipasang secara random langsung di lokasi padang penggembalaan, sedangkan di kandang, perangkap ditempatkan pada jarak 3-5 meter dari pagar pembatas kandang. Perangkap dipasang pada jarak kurang lebih 10-15 cm di atas permukaan tanah. Pengumpulan sampel lalat dilakukan dalam interval waktu satu jam sejak pemasangan, yaitu pukul 09;00, 10;00, 11;00, 12;00, 14;00 dan pukul 15;00. Lalat dikumpulkan dan dieuthanasia dengan alkohol 70%, kemudian di identifikasi menurut spesies dengan kunci identifikasi menurut Scott et al (1967) dan Dodge (1967), selanjutnya data dianalisa secara deskriptif.
Co-Authors Aven B Oematan Filphin Adolfin Amalo Foeh, Nancy Diana Gerson Yohanes Imanuel Sakan Hermilinda Parera Koten, Bernadette B. Moenek, Devi Y.J.A. Muhammad Mirandy Pratama Sirat Nemay Anggadewi Ndaong Ni Sri Yuliani Nitbani, Henny Novianti Neliyani Toelle Simarmata, Yohanes T. R. M. R. Tarsisius Considus Tophianong, Tarsisius Considus Tri Wahyu Pangestiningsih Woro Danur Wendo Yulfia N Selan Yulfia Nelymalik Selan