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All Journal Jurnal Ilmu Ternak Indonesian Journal of Biotechnology JURNAL ILMIAH PETERNAKAN TERPADU JURNAL KAJIAN VETERINER
Victor Lenda
Program Studi Kesehatan Hewan Jurusan Peternakan Politeknik Pertanian Negeri Kupang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary Other

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GAMBARAN PATOLOGI ANATOMI PADA BABI LANDRACE SUSPECT AFRICAN SWINE FEVER (ASF) DI KABUPATEN KUPANG Simarmata, Yohanes T. R. M. R.; Tophianong, Tarsisius Considus; Amalo, Filphin Adolfin; Nitbani, Henny; Lenda, Victor
JURNAL KAJIAN VETERINER Vol 8 No 2 (2020): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v8i2.3074

Abstract

African Swine Fever (ASF) is a viral disease that attacks pigs and to date has caused many pig deaths in Kupang Regency. ASF is caused by a double-stranded DNA virus from the Asfivirus genus and the Asfarviridae family. This research aims to determine the anatomical pathology of the swine landrace suspect ASF. Organ samples were collected from two male landrace pigs and two female landrace pigs, aged 7 months, from Oeltuah Village, Taebenu District and Tarus Village, Central Kupang District, Kupang Regency, NTT. Clinical examinations were carried out on sick animals that were found during the investigation, then necropsied on the dead animals were carried out and continued with anatomical pathology examinations at the Pathology Laboratory, Faculty of Veterinary Medicine, Nusa Cendana University. Anatomical pathology examinations are carried out by observing changes in the structure and appearance of the organs. The necropsy results showed sub-cutaneous ecchymosis hemorrhage in the abdomen, limbs and ears, gastric, intestinal and hepatic hemorrhage, hemorrhagic lymphadenitis in mesenteric lymph nodes, hyperemic splenomegaly, pteckie hemorrhage in the renal capsule,, multifocal hemorrhage in the renal medulla and pulmonary lobe. Based on the observation of clinical symptoms and changes in anatomical pathology, it can be concluded that the death of pigs was suspected to be caused by the suspect ASF.
Evaluasi Kerusakan DNA Spermatozoa Semen Cair Babi Menggunakan Pewarnaan Toluidine Blue Parera, Hermilinda; Lenda, Victor; Foeh, Nancy Diana; Sirat, Muhammad Mirandy Pratama
JURNAL KAJIAN VETERINER Vol 12 No 1 (2024): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v12i1.14732

Abstract

The integrity of the deoxyribonucleic acid (DNA) in sperm plays a crucial role in the fertilization process and embryonic development. This study aimed to evaluate the level of the DNA fragmentation of boar sperm in tris-citrate-fructose extender preserved at temperatures between 16-18°C for 3 days. The level of sperm DNA damage was analysed using toluidine blue (TB) staining. Pig semen collected from 4 male pigs aged 1.3-1.8 years was used as the research sample. Semen collected was conducted twice a week. The experimental method using a Completely Randomized Design with 4 storage duration groups as follows: Group I (0 hours of storage), Group II (24 hours of storage), Group III (48 hours of storage), and Group IV (72 hours of storage), each with 5 replications. The data were analysed using analysis of variance (ANOVA) and, if significant differences were found, Duncan's test was performed. The results of the evaluation of the percentage of DNA damage during the storage process at 16-180C at 0 hours, 24 hours, 48 hours and 72 hours was: 0.33 ± 1.66%, 0.80 ± 0.447%, 1.60 ± 1.14% and 2.80 ± 0.447%, respectively. These results demonstrate that liquid boar semen preserved with tris citrate fructose extender at 16-18 °C for 3 days has spermatozoa DNA damage levels within the normal range, making it suitable for artificial insemination and ensuring optimal AI success.
Effects of Various Tris Citrate Fructose-based Extenders on Plasma Membrane Integrity, Sperm DNA Fragmentation, and Artificial Insemination Success in Pigs Lenda, Victor; Parera, Hermilinda; Foeh, Nancy Diana; Sirat, Muhammad Mirandy Pratama
JURNAL KAJIAN VETERINER Vol 13 No 1 (2025): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v13i1.19526

Abstract

Keberhasilan reproduksi merupakan faktor kunci dalam meningkatkan efisiensi dan produktivitas usaha peternakan babi. Salah satu strategi yang digunakan untuk mendukung keberhasilan tersebut adalah inseminasi buatan (IB), yang sangat bergantung pada kualitas semen selama penyimpanan. Penelitian ini bertujuan untuk mengevaluasi integritas membran plasma (IMP), fragmentasi DNA spermatozoa, tingkat keberhasilan IB, dan litter size menggunakan semen babi yang diencerkan dengan berbagai jenis pengencer berbasis tris sitrat fruktosa (TCF), selanjutnya disimpan pada suhu 16 °C hingga 72 jam. Penelitian dilakukan dalam dua tahap. Tahap pertama menilai kualitas semen, yaitu PMI dan fragmentasi DNA, menggunakan rancangan acak lengkap dengan lima ulangan. Tiga jenis pengencer yang diuji adalah TCF + ekstrak mesokarp + kuning telur (TCF+EM+KT), TCF + ekstrak mesokarp (TCF+EM) dan TCF, masing-masing pada penyimpanan 24, 48, dan 72 jam. Tahap kedua mengevaluasi keberhasilan IB pada 36 ekor induk babi hasil persilangan Duroc-Landrace berumur 1–3 tahun, menggunakan semen yang disimpan selama 72 jam. Parameter yang diamati meliputi conception rate (CR), service per conception (S/C), non-return rate (NRR), dan ukuran litter. Hasil penelitian menunjukkan bahwa pengencer TCF+EM+KT memberikan hasil terbaik, dengan nilai IMP tertinggi (97,4±0,43%), fragmentasi DNA terendah (0,40±0,55%), CR tertinggi (83%), S/C terendah (1,17), serta rata-rata litter size terbesar (7,42), secara signifikan berbeda (P<0,05) dibandingkan pengencer lainnya. Kesimpulan penelitian ini menunjukkan pengencer TCF+EM+KT paling efektif dalam mempertahankan kualitas semen dan meningkatkan performa reproduksi pada IB babi.
Co-Authors Aven B Oematan Filphin Adolfin Amalo Foeh, Nancy Diana Gerson Yohanes Imanuel Sakan Hermilinda Parera Koten, Bernadette B. Moenek, Devi Y.J.A. Muhammad Mirandy Pratama Sirat Nemay Anggadewi Ndaong Ni Sri Yuliani Nitbani, Henny Novianti Neliyani Toelle Simarmata, Yohanes T. R. M. R. Tarsisius Considus Tophianong, Tarsisius Considus Tri Wahyu Pangestiningsih Woro Danur Wendo Yulfia N Selan Yulfia Nelymalik Selan