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All Journal BALI MEDICAL JOURNAL (BMJ) Jurnal Berkala Epidemiologi Jurnal NERS Health Notions Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Journal Orthopaedi and Traumatology Surabaya (JOINTS) Jurnal Layanan Masyarakat (Journal of Public Service) Indian Journal of Forensic Medicine & Toxicology Narra J The Hip and Knee Journal
Mouli Edward
Department Of Orthopaedic And Traumatology, Faculty Of Medicine, Universitas Airlangga – Dr. Soetomo General Hospital, Surabaya
Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Dentistry Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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The Effect of Culture Techniques of Hypoxic Stem Cell Secretome on The Number of Growth Factor TGF-β, BMP-2, VEGF Edward, Mouli; Mahyudin, Ferdiansyah; Manyakori, Dionysius Bramta Putra
(JOINTS) Journal Orthopaedi and Traumatology Surabaya Vol. 11 No. 1 (2022): April 2022
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/joints.v11i1.2022.5-9

Abstract

Background: Mesenchymal stem/stromal cell (MSC) therapy is now an effective therapeutic modality for treating various diseases. In its application, stem cells require signaling molecules, including growth factors, cytokines, and chemokines. Signaling molecules function in an orderly manner and are greatly influenced by the physiological environment. Stem cell culture techniques with hypoxic conditions can produce growth factors similar to those found in fracture conditions. This study aimed to evaluate the differential expression of growth factors in cultured normoxic and hypoxic bone marrow stem cell (BMSCs).Methods: This in vitro laboratory experimental study examined normoxic and hypoxic BMSC cultures. BMSCs were harvested from rabbits, propagated in vitro, and cultured under normoxic and hypoxic conditions. Vascular endothelial growth factor (VEGF), Transforming growth factor-β (TGF-β), and Bone morphogenetic protein-2(BMP-2) levels were measured using ELISA.Results: VEGF, TGF-β, and BMP-2 expression showed significant differences between the normoxia and hypoxia groups. The VEGF, TGF-β, and BMP-2 expressions were higher in the hypoxia group compared with the normoxia group (p < 0.05).Conclusion: The expression of TGF-β1, VEGF, and BMP-2 growth factors in cultured BMSCs was significantly different between normoxic and hypoxic conditions. TGF-β1, VEGF, and BMP-2 expression increased under hypoxic conditions.
Growth Factor Comparison in Cortical Demineralized Bone Matrix that Demineralized Using Chloric and Acetic Acid Edward, Mouli; Mahyudin, Ferdiansyah; Basuki, Mohammad Hardian; Suroto, Heri; Perwira, Ferdiansyah Danang
(JOINTS) Journal Orthopaedi and Traumatology Surabaya Vol. 12 No. 1 (2023): April 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/joints.v12i1.2023.1-9

Abstract

Background: Demineralized bone matrix (DBM) is an alternative biomaterial for which specific acid and immersion time are needed to optimize growth factor preservation. The optimal demineralization protocol for preserving growth factors in DBM remains unclear. This study investigated DBM extraction methods using different acids and immersion times to maintain optimal growth factor preservation.Methods: This in vitro experimental laboratory study used a randomized controlled post-test-only group design. We characterized the Insulin-like growth factor-1 (IGF-1), Bone morphogenetic protein-2 (BMP-2), and Transforming growth factor-β (TGF-β) content of 1 gram of New Zealand White Rabbit cortical bone immersed in 0.6 M hydrochloric acid and 0.5 M acetic acid for 3, 6, and 9 days. We then analyzed the differences in growth factor levels in each acid and performed statistical analysis.Results: IGF-1 levels were higher in DBM demineralized with acetic acid than with hydrochloric acid. BMP-2 and TGF-β levels were higher in DBM demineralized using hydrochloric acid. The concentration of growth factors decreased over time in DBM demineralized using acetic acid. The highest growth factor level was obtained after 6 days of immersion in hydrochloric acid.Conclusion: DBM demineralized with acetic acid yielded higher average IGF-1 levels compared to hydrochloric acid. However, BMP-2 and TGF-β levels were higher with hydrochloric acid. Growth factor levels in hydrochloric acid peaked at 6 days and then decreased. These results suggest that avoiding over-demineralization is important for maintaining growth factor levels. Further research is needed to optimize DBM processing.
ENHANCING KNOWLEDGE AND EDUCATION OF BONE CANCER FOR ADOLESCENT IN INDONESIA Abdul Bari, Yunus; Mahyudin, Ferdiansyah; Edward, Mouli; Basuki, Mohammad Hardian; Martanto, Tri Wahyu; Wardhana, Teddy Heri; Suyono, Rifki Effendi; Purnamasari, Yunita; Permana, Muhammad Dimas Arya Candra; Ardana, Fahrizal Novan
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 9 No. 1 (2025): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v9i1.2025.019-025

Abstract

Despite much-advanced therapy developed for bone cancer, prevention of the disease remains the most favorable and low-cost management approach. Bone cancer prevention and education should be initiated with adolescents.  Adolescents are the right group to receive bone cancer education, as they are in an active learning phase and have the potential to develop risky behaviors that can increase vulnerability to cancer, as they are in a stage of active learning but also face the development of risky and unhealthy behaviors. This study aims to assess the knowledge of bone cancer among adolescents in Indonesia. It is a cross-sectional study that uses a questionnaire to evaluate the participants' knowledge of bone cancer. A total of 39 questions regarding family history and general knowledge of cancer are given to the students in a pre-test and post-test format. Between the tests, a group of orthopedic specialists provided a short lecture to the students about the basic knowledge and prevention of bone cancer. The study involved 564 students from junior and senior high schools in Indonesia, with an overall mean age of 14.79 years. Bone cancer was mentioned in the family history of only 0.4% of the participants, but 21.5% to 23.5% were unsure about their own family cancer status. The study found a significant statistical result, indicating that the short lectures given by the orthopedic specialists were effective in enhancing the students' knowledge about bone cancer. The findings suggest that adolescents in Indonesia are still uncertain about their family cancer status and often misinformed about the symptoms of bone cancer. However, an educational lecture or discussion led by an expert in the field has proven to be an effective way to improve the students' knowledge about bone cancer.
Triple Local Recurrence of Synovial Sarcoma: A Case Report Edward, Mouli; Akbar, Kukuh Ali
(JOINTS) Journal Orthopaedi and Traumatology Surabaya Vol. 12 No. 2 (2023): October 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/joints.v12i2.2023.83-87

Abstract

Background: Synovial Sarcoma (SS) hardly appears on non-extremity sites (20% of cases). Even rarer is a local recurrence, which occurs between 10-30% of cases. Here, we present a rare case of hip Synovial Sarcoma, which recurs three times.Case Report: A 33-year-old male came to our outpatient clinic with chief complaints of a sudden ingrowing mass on his left hip. The lump is the size of a chicken egg and doesn't get bigger over time. There was palpable tenderness felt whenever he touched the lump. The patient was diagnosed with SS of the left hip with IIB stage from additional radiographic and histopathologic examination. Surgical-wide resection had been done three years before the visit, and chemotherapy six times. The lump reappeared one year before the visit, and the patient underwent a second surgery. The mass re-surfaced within the same region on the current visit for the third time, and he underwent the same surgical treatment afterward. Two years after the third surgery and adjuvant chemotherapy, the patient had no complaint about a lump and planned a radiograph evaluation.Discussion: Detailed examination from history taking, physical examination, histopathologic, and radiological results is important to determine appropriate staging. With proper staging, physicians can better understand disease prognosis and risk of recurrence or metastases.Conclusions: The third recurrence may still happen on SS. Its unique location might contribute to this unique occurrence, and the size of the tumor makes the recurrence rate higher.
Co-Authors Abdul Bari, Yunus Akbar, Kukuh Ali Ansari Rahman Ardana, Fahrizal Novan Arifin Arisvia Sukma Hariftyani Arius Suwondo Armando, Braven Asyumaredha Asyumaredha Bayu Antara Hadi Benedicta, Steesy Buwana, Dewan S. Caesar Haryo Bimoseno Deny Mory Aryawan Desnantyo, Andre Triadi Dwikora Novembri Utomo Erfan Nasrullah Fedik Abdul Rantam Ferdiansyah Mahyudin Fikri Rasikh Pritanto Henry Dominica Heri Suroto Hermina Novida, Hermina I Putu Gede Pradnyadewa Pradana Kadek Seta Prawira Kukuh Dwiputra Hernugrahanto Laskar Pradnyan Kloping, Laskar Pradnyan Lisan, Rizal A. Lukas Widhiyanto M Hardian Basuki Manyakori, Dionysius Bramta Putra Martanto, Tri Wahyu Mohammad Hardian Basuki Muhammad Dimas Arya Candra Permana, Muhammad Dimas Arya Candra Muhammad Hardian Basuki Muhammad Hardian Basuki Muhammad Hardian Basuki Muhammad Hardian Basuki P. Purwati, P. Perwira, Ferdiansyah Danang Putu Garry Rizky Agung Satria Rwahita Satyawati Dharmanta Setiawati, Rosy Sjahjenny Mustokoweni, Sjahjenny Steesy Benedicta Suyono, Rifki Effendi Wardhana, Teddy Heri Wardhani, Indrayuni Lukitra Yesa Suwandani Yunita Purnamasari, Yunita Yunus Abdul Bari Yunus Basrewan