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All Journal Jurnal Penelitian Saintek Bioma : Berkala Ilmiah Biologi AL KAUNIYAH Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Pharmaciana: Jurnal Kefarmasian Eksergi: Chemical Engineering Journal Journal of Natural Sciences and Mathematics Research Jurnal Kimia Riset Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Biosaintifika: Journal of Biology & Biology Education Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) JKPK (Jurnal Kimia dan Pendidikan Kimia) International Journal of Active Learning Indigenous Biologi Bioed : Jurnal Pendidikan Biologi BIOCHEPHY: Journal of Science Education Jurnal Ilmiah Sains Gunung Djati Conference Series Engagement: Jurnal Pengabdian Kepada Masyarakat Biosaintifika: Journal of Biology & Biology Education Jurnal Pengabdian Kepada Masyarakat
Elizabeth Betty Elok Kristiani
Universitas Kristen Satya Wacana
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Other

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Journal : Biosaintifika: Journal of Biology

 1 
The Phytochemical and Biological Activities of Two Phyllanthus Species: Insights into Metabolit, Antioxidant and Antibacterial Activity Araújo, Otilda Fátima; Kristiani, Elizabeth Betty Elok; Kasmiyati, Sri; Nugroho, Laurentius Hartanto
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 3 (2024): December 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i3.13944

Abstract

Phylanthus species, including P. niruri and P. urinaria have pharmacological potential due to their rich phytochemical composition. People usually used this plant for medicinal treatments. This study aimed to compare the phytochemical and antioxidant and antibacterial activities of two Phyllanthus species. The whole of plants was extracted using maceration method with ethanol as solvent. Phytochemical content analyzed using spectrophotometer.  The reagent used for each compounds that were Folin-ciocalteu for phenolics, AlCl3 for flavonoid, dimethyl sulfoxide for chlorophyll and carotenoid. Bioactivity analysis using 1,1-diphenyl-2-picrylhydrazyl method for antioxidant activity and Kirby-Bauer method for antibacterial activity. The highest flavonoid content (12.22 mg QE/gram extract) and total chlorophyll (43.2 µg/ml extract) in P. niruri while phenolic content (80.8 mg GAE/gram extract) in P. urinaria. The carotene of both Phyllanthus were similar (11.9 µg/ml extract. The IC50 values of P. urinaria (6.16 ± 0.42 μg/ml) and P. niruri (17.72 ± 0.80 μg/ml), which indicated very strong antioxidant activity. P. urinaria leaf extract had stronger inhibition against Escherichia coli than Staphylococcus aureus (>20 mm) and P. niruri leaf extract could inhibit E. coli and S. aureus bacteria at all concentrations (11-20 mm). This study found that phenolic compounds strongly influenced the antioxidant and antibacterial abilities of Phyllanthus, while chlorophyll and carotenoids had only a slight influence. These findings open up opportunities to utilize P. niruri and P. urinaria as antioxidant and antibacterial agents.
Extract Formulation of Zingiber officinale and Phylanthus reticulatus Induces Apoptosis of WiDr Colon Cancer Cells Kristiani, Elizabeth Betty Elok; Kasmiyati, Sri; Martono, Yohanes; Kachingwe, Baxter Hepburn
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 3 (2025): Inpress December 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i3.25776

Abstract

People use ginger (Zingiber officinale) extensively for health purposes. Our previous studies have shown that Phyllanthus reticulatus has strong antioxidant capabilities. This study investigates the anticancer potential of ethanolic extract of Z. officinale and P. reticulatus formulations (ZP), focusing on their cytotoxicity and mechanisms against cancer cells. Samples were extracted using the maceration method. The method for determining the cytotoxicity of cells is MTT, the cell cycle is flow cytometry, apoptotic cells are flow cytometry and double staining, and caspase-8 and caspase-9 expression using the immunocytochemistry method. The analysis of caspase-8 and caspase-9 expression using the immunocytochemistry method. Among the tested formulations, the ethanolic extract of ZP_1:0 showed the most promise against WiDr colon cancer cells (IC50 value of 102.1 µg/ml). The ZP_1:0 demonstrated dose-dependent apoptosis induction in WiDr cells, showing early and late apoptosis, with minimal necrosis. The extract activates extrinsic and intrinsic apoptotic pathways in inducing cancer cell death. Compared to doxorubicin, the ZP 1:0 formulation selectively promotes apoptosis with reduced necrotic damage, suggesting the potential for reduced systemic toxicity. The study supports the potential of Z. officinale as a source of safer, plant-based anticancer agents, particularly for colon cancer, while recommending further research to identify and enhance active compounds.
Co-Authors ,, Djohan Agna Sulis Krave Agung Rimayanto Gintu Agung Rimayanto Gintu Agung Rimayanto Gintu Alberthnico, Ferdian Andreas Binar Aji Sukmana Anggara Mahardika Araujo, Otilda Fatima Ari Hasna Widyapuspa Cahyaningrum, D C. Cahyaningrum, Desti Christian Djurubasa, Dani Karel Marthin Egarani, Grahita Rezhi Grahita Rezhi Egarani Hartati Sulastri Hastuti, S P. Ie, Johanes Juan Yutama Putra Indrianasari, Putri Jeanelsin A.P Langgori Kachingwe, Baxter Hepburn Kasmiyati, S Keliat, Natalia Rosa L. Hartanto Nugroho Lilik Linawati Maria Marina Herawati Nabila, Nabila Natalia Rosa Keliat Priyayi, Desi Fajar Purwoko, Agus Putra Ie, Johanes Juan Yutama Putri, A C. Rahardja, P M. Ramagita, Marsahanda Astri Rimanti, Audri Septina Putri Rully Adi Nugroho Setiyarini, Chatarina Titik Siahaya, Esty Vicariat Sri Kasmiyati Sri Yulianto Sucahyo Sucahyo, S Trisianna, Jessica Ayu Trisianna Utomo, Daniel Setyo Vincentia Irene Meitiniarti Wardani, Yulia Kusma Wicaksono, Rifky Adi Yayi Suryo Prabandari Yohanes Martono Yusta, G. Maria