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All Journal Jurnal Veteriner JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Jurnal Biologi Tropis Jurnal Pengabdian Magister Pendidikan IPA JITPI : Jurnal Ilmu Dan Teknologi Peternakan Indonesia (Indonesian Journal of Animal Science and Technology) Jurnal Abdi Insani Jurnal Triton Jurnal Pepadu
Enny Yuliani
Universitas Mataram
Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Social Sciences Veterinary

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Journal : Jurnal Veteriner

 1 
SPECIFIC PROTEIN EXPRESSION OF GOAT PREIMPLANTATION EMBRYO IN VITRO AS AN EARLY PREGNANCY SIGNAL I Wayan Lanus Sumadiasa; Enny Yuliani
Jurnal Veteriner Vol 9 No 2 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of the experiment was to investigate the intensity of signal secreted by embryos for their survival in the early pregnancy. Embryos at morulla and blastocyst stages generated by in vitro fertilization were frozen and then thawed for in vitro culture in tissue culture medium. The protein secreted by embryo were detected 48 hours after thawing for those frozen at morulla stage, and 24 hours and 72 hours after thawing for those frozen in the blastocyst stage. Results showed that preimplantation goat embryos secreted an average of 3,041 µg protein per ml medium for the embryo cultured in tissue culture medium (TCM) 199 + 0,1 % BSA and 1,992 µg protein per ml medium for embryo cultured in TCM without BSA. Non growing embryos secreted only a small amount protein, i.e. 0,434 µg protein per ml medium and 0,417 µg protein per ml medium respectively for embryo cultured TCM 199 with BSA and TCM without BSA. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis showed protein bands with many different molecular weight of 100, 95 , 55 , 43 , 28 and 18 KD. It appeared that the growing embryos secreted more proteins as compared to those non growing embryos.
Co-Authors Adji Santoso Dradjat Adji Santoso Dradjat Adji Santoso Dradjat Aldian Maulana Bulkaini (Bulkaini) Caska - Chairussyuhur Arman Chairussyuhur Arman Djoko Kisworo Elsya Mayori Gde Bidura I Wayan Lanus Sumadiasa Jurnal Pepadu Lalu Ahmad Zaenuri Lalu Ahmad Zainuri Lalu Ahmad Zainuri Lalu Wirapripadi Lukman HY Mardiansyah Mardiansyah Mardiansyah Mardiansyah Mardiansyah Mardiansyah Maskur Maskur Muhammad Haikal Fikri Mujahidurrohman Mujahidurrohman Nifsu Sabaan Prasetyo Nugroho Putra, Ryan Aryadin Rodiah Rodiah Rodiah Rodiah Rodiah Rodiah Rodiah Rodiah Rodiah Rodiah Rodiah Sugeng Prasetyo