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All Journal JMM (Jurnal Masyarakat Mandiri) Biota Abdinesia: Jurnal Pengabdian Kepada Masyarakat Medika: Jurnal Ilmiah Kesehatan
Baiq Repika Nurul Furqan
Departement Of Pharmacy, Universitas Nahdlatul Ulama Nusa Tenggara Barat
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Journal : Biota

 1 
The Genetic Diversity of Macroalgae on The Ekas Coast of East Lombok Based on Chromatogram Data from PCR Ampification Baiq Repika Nurul Furqan; Imam Syahputra Yamin; Sri Sofiati Umami
Biota Vol 16 No 1 (2023)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v16i1.417

Abstract

Macroalgae are multicellular photosynthetic organisms that live in water. Some species of macroalgae are very difficult to identify based on their morphology alone because they have great similarities. Therefore, identification of macroalgae diversity based on its genotype is very important to do in order to enrich the genetic information of macroalgae found on one of the beaches on the island of Lombok. This study aimed to analyze the genetic diversity of macroalgae from Pantai Ekas Beach, Jeowaru District, East Lombok, NTB using 18S rRNA gene markers. The research was carried out in 4 stages, namely sampling, morphological analysis of samples, isolation of total macroalgae DNA, isolation of genes by PCR amplification, agarose gel electrophoresis. Sampling was carried out at certain coordinates at east longitude and south latitude using the Ekas Beach quadratic transect method. The morphological analysis of the samples was guided by an identification key. Total DNA, macroalgae were isolated based on the spin column I DNA kit easy method. Genes were isolated by means of PCR amplification. Total DNA isolates and gene amplicons were confirmed by agarose gel electrophoresis. The results obtained were based on their morphology, all samples obtained were grouped into 9 macroalgae samples which could be divided into brown macroalgae (3 samples), green macroalgae (2 samples) and red macroalgae (4 samples). All samples have successfully isolated total DNA and its 18S rRNA gene which was confirmed by agarose gel electrophoresis chromatogram.
Optimization of Biodiesel Production from Sludge of Mask Waste and Used Cooking Oil Using Lipase as Biocatalyst Muhammad Rizal Januardi; Baiq Repika Nurul Furqan
Biota Vol 16 No 1 (2023)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v16i1.423

Abstract

Mask waste and used cooking oil contain hydrocarbons and free fatty acids. the combination of these ingredients with pretreatment heating in the microwave and the help of lipase enzymes makes the resulting sludge has the potential to be converted into biodiesel with a maximum yield when compared to making biodiesel from other materials and methods. The results of this study indicate that pre-treated sludge from mask waste and used cooking oil can be converted into biodiesel through a methanol transesterification reaction with the help of thermostable lipase enzymes from Pseudomonas. As for the optimum conditions, the highest biodiesel yield was produced at a temperature of 60℃, the best reaction time was 25 minutes, the volume of sludge was 10 mL and the optimum pH was at pH 8. The flame test on the biodiesel produced had been carried out, most of which had bright blue, red flames and orange. There are also some results that the flame is dim and even the test results are not lit.
Making Liquid Sugar Preparations from Lombok-Traditional Sugarcane Juice and Its Potential as Anti-Diabetic Sugar Nurul Furqan, Baiq Repika; Maharani, B Fitria
Biota Vol 15 No 1 (2022)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v15i1.407

Abstract

The prevalence of Patients with Diabetes Mellitus remains on the rise worldwide. Diabetes is a multifactorial disorder that emboils genetic and environmental factors such as an unhealthy diet by consuming white sugar crystals. White crystal sugar consists of less chromium content that induces glucose in the blood to the cells, which can trigger hyperglycemia or high blood sugar levels. Chromium works by increasing the activity of insulin receptors to enhance glucose translocation to the cell. Previous studies showed sugarcane Nira had a high level of chromium compared with those processed into white crystalline sugar. However, it could not be used as a sweetener substitute since its storage age was only 2 to 3 days. This study identified that the treatment process of Lombok's Nira sugarcane into local liquefied sugar should be addressed to maintain chromium levels compared to the processing of Nira into the white crystalline sugar requiring optimum sucrose and omitting several minerals. Hence, sugarcane Nira as a refined sugar product can substitute for white sugar consumption in diabetes patients.
The Concentration Variation Effect of Earring Leaves (Acalypha Indica L.) Extract on It’s Bioactivity Ability in Inhibiting The Growth of Salmonella Typhi (S. Typhi) Ziana, Elma; Furqan, Repika Nurul; Nurul Furqan, Baiq Repika
Biota Vol 15 No 2 (2022)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v15i2.485

Abstract

The research has been carried out on the effect of variations in the concentration of earring leaves (Acalypha Indica L.) ethanol extract on the acquisition of inhibition zone values for the growth of S. typhi bacteria. In this study, ethanol solvent was used in the maceration and evaporation stages, then the well diffusion method was used to test its antibacterial activity. Each stage was carried out in triplo and 3 repetitions. Phytochemical test on the evaporated extract showed positive test results for flavonoid and tannin metabolite compounds. Concentration variations that used in this study were 5%, 15%, 30%, 50% and 60%, the antibiotic chloramphenicol was also used as a positive control and aquadest as a negative control. Meanwhile, the antibacterial activity test gave the result that the higher the concentration of the earring leaf extract (Acalypha Indica L.), the inhibition zone value also increased with the following successive values: 0mm, 11mm, 14mm, 15mm and 16mm, on the other hand chloramphenicol showed an inhibition zone of 32 mm. The research data were analyzed statistically using the One Way Anova test. Earring leaf extract (Acalypha Indica L.) has a significant effect of 0.000 on S. Typhi bacteria. However, at the smallest concentration, namely 5%, it does not have an inhibition zone diameter of 0 mm with a significant value of 1000. It can be concluded that the antibacterial activity using chloramphenicol is still higher when compared to the antibacterial activity using the ethanol extract of earring leaves (Acalypha Indica L.) in inhibiting growth of S. Typhi bacteria.
Test Of Antibacterial Activity Of Ethanol Extract Of Maman Lanang Plant (Cleome Rutidospermae Dc ) Against Gram Positive And Negative Bacteria (Staphylococcus aureus and eschericihia coli) Nurul Furqan, Baiq Repika; Sanjaya, Denny; Almahera, Almahera; Maharani, B Firia
Biota Vol 15 No 2 (2022)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v15i2.486

Abstract

The Maman Lanang plant ( Cleome rutospermae dc ) is a weed plant that is often found in rice fields or grows on community cultivated plants which are usually used as food for people on the outskirts. This research aims to determine the effect of the extract concentration of the Maman Lanang Plant ( Cleome rutospermae dc ) on the inhibition zone for the growth of Staphylococcus aureus and Escherichia coli bacteria, as well as to find out how much the concentration of the extract from the Maman Lanang Plant ( Cleome rutospermae dc ) is close to the inhibitory zone value of the positive control of tetracycline in inhibiting the growth of Staphylococcus aureus and Escherichia coli bacteria . This research is a laboratory experimental study using a Completely Randomized Design (CRD) using the hole/cup diffusion method with four varying concentrations, namely 10%, 30%, 60% and 90%, and the positive controls used are tetracycline and distilled water as a negative control. The main ingredient used in this research was the Maman Lanang plant ( Cleome rutospermae dc ) which was extracted using the maceration method for three days with 96% ethanol solvent, after that the extract obtained was then subjected to an evaporator to obtain the crude extract then carried out a phytochemical test and after that The antibacterial activity of the crude extract from the Maman Lanang Plant ( Cleome rutospermae dc ) was tested against the bacteria Staphylococcus aureus and Escherichia coli . Then the data obtained was analyzed using the antibacterial ANOVA ( Analysis of variance ) method. The results of this study show that phytochemical test analysis shows the presence of flavonoid compounds in extracts from the Maman Lanang Plant ( Cleome rutospermae dc ). Furthermore, the analysis showed that variations in concentration of the Maman Lanang Plant ( Cleome rutospermae dc ) had an influence on the antibacterial activity of Staphylococcus aureus and Escherichia coli. Where the higher the concentration of the extract, the larger the inhibition zone will be formed. The 90% concentration of the extract showed the largest inhibition zone with a diameter of 23.3 mm.
Antibacterial Activity Test of Ethanol Extract of Red Macroalgae (Acanthopora Spicifera) Against Staphylococcus Aureus Bacteria Taibin, Ali; Yamin, Imam Syahputra; Nurul Furqan, Baiq Repika
Biota Vol 15 No 2 (2022)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v15i2.487

Abstract

Bacterial infection Staphylococcus aureus has become a significant global health problem, mainly due to increasing resistance to existing antibiotics. To overcome this problem, research is continuing to find new sources of natural antibacterial agents. Red macroalgae (Acanthopora sp.) has promising biological potential, including activity as an antimicrobial. This study aims to determine the effect and how much concentration of red macroalgae ethanol extract (Acanthopora sp.) which is close to the value of the inhibition zone of the positive control Amoxicillin in inhibiting bacterial growth S. aureus. This research is a laboratory experimental research. The stages of the research began with taking red macroalgae samples (Acanthopora sp.) collected from Pandanan beach, North Lombok Regency, West Nusa Tenggara by the method simple random sampling and identified morphologically, manufacture of simplicia, manufacture of red macroalgae ethanol extract (Acanthopora sp.) with 99.99% ethanol solvent (absolute p.a), phytochemical screening tests, and antibacterial activity tests using a Completely Randomized Design (CRD) were carried out by well diffusion method with concentration variations of 2%, 4%, 6%, 8% and 10%, Amoxicillin as positive control and aquadest as negative control and ethanol as solvent control. Antibacterial activity test results from the crude extract of red macroalgae (Acanthopora sp.) against bacteria S. aureus incubated during overnight (16-18 hours) in an incubator with a temperature of 37OC. The results showed that the phytochemical analysis of the ethanol extract of red macroalgae identified the presence of flavonoids and saponins which are known to have potential as antibacterial agents. The presence of these compounds supports the antibacterial activity of red macroalgae against S. aureus. Red macroalgae ethanol extract (Acanthopora sp.) has antibacterial activity against S. aureus. The antibacterial activity of the ethanol extract showed a concentration-response pattern, where the higher the concentration of the extract, the greater the inhibition zone formed. The concentration of 10% ethanol extract showed the largest inhibition zone with an average diameter of 23.5 mm with the sensitive category.
Bioactivity Diversity (Antibacterial and Antioxidant) of Macroalgae in the Ekas Beach Area, Lombok Nurul Furqan, Baiq Repika; Yamin, Imam Syahputra; Umami, Sri Sofiati
Biota Vol 17 No 1 (2024)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v17i1.489

Abstract

Macroalgae in the southern coastal area of Lombok has not been explored much in terms of bioactivity. The aim of this research is to explore the usefulness of 9 types of macroalgae on Ekas Lombok beach by searching for their bioactivity capabilities, whether each macroalgae has no bioactivity at all, has antibacterial and antioxidant activity, only has antibacterial activity but no antioxidant activity or vice versa and whether there are macroalgae that even have anticancer activity. The method used in this research consists of several steps: Macroalgae Sampling, Morphological Analysis of Macroalgae Samples, Isolation of active compounds from macroalgae and Cytotoxic Test on Breast Cancer Cells in Vitro which consists of 3 tests: Culture and sub culture of MCF-7 Breast Cancer Cells, Cell Viability and Cytotoxicity Test with MTT and DNA Isolation of MCF-7 Breast Cancer Cells. The research results show that the macroalgae with the best antibacterial activity is the red macroalgae Acanthophora spicifera and the macroalgae with the best antioxidant activity is the green macroalgae Ulva reticulata.
Identification of the Heavy Metal Lead (Pb) in Red Macro Algae (Gracilia sp) In the waters of Tanjung Luar, East Lombok Almahera, Almahera; Kurniawan, Ahmad Aldi; Maharani, B Firia; Himayanti, Yusfina Firia; Puspita, Novia Dara; Nurul Furqan, Baiq Repika
Biota Vol 17 No 2 (2024)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Tanjung Luar Village is located in Keruak District, East Lombok Regency, West Nusa Tenggara, is a densely populated body of water human activity. In Tanjung Luar waters, household and ship waste dumped directly into the sea, polluting the local ecosystem. this condition important because people in the area consume a lot of produce marine catches, including red macroalgae (Gracilaria Sp). If Gracilaria Sp. is contaminated with levels of the heavy metal lead (Pb) that exceed threshold of 0.2 mg/kg, can be dangerous to human health impact on the nervous system, urinary system, endocrine system, problems gastrointestinal, and a very high risk of cancer. That thing based on BPOM Regulation No. 23 of 2017 (<0.2 mg/kg) for consumption materials. Therefore pollution and controlling the use of the heavy metal lead (Pb) important, especially in waters that play a supporting role life and food of local communities. The aim of this research is to determine the levels of the heavy metal lead (Pb) contained in the sample red macroalgae (Gracilaria Sp.) in Tanjung Luar Waters. Purposeful research to provide an overview and explanation of the issues discussed. This research uses the wet digestion method to identify heavy metal lead (Pb) of Gracilaria sp in waters Tanjung Luar, East Lombok. Next, levels of the heavy metal lead (Pb) were tested with ICP-OES spectrophotometry. With this method, researchers can identify the concentration of the heavy metal lead (Pb) in Gracilaria Sp. with a high level of accuracy. The results of the study showed that all sampling points of Gracilaria sp. did not exceed the threshold, which was less than 2 mg/kg. where at the sampling location point 1 had Pb concentrations of 0.072 ppm (location A), 0.063 ppm (location B), 0.057 ppm (location D), 0.051 ppm (location E) and 0.046 ppm (location F). for point 2 had Pb concentrations of 0.096 ppm (location A), 0.083 ppm (location B), 0.071 ppm (location C), 0.065 ppm (location E) and 0.059 ppm (location F). for point 3, the Pb concentrations were respectively 0.142 ppm (location A), 0.108 ppm (location C), 0.097 ppm (location D), 0.084 ppm (location E) and 0.070 ppm (location F)
Co-Authors Afrianti, Mahram Almahera, Almahera DENNY SANJAYA Elinda, Yusnaini Fikri, Haikal Furqan, Repika Nurul Halqi, Muhammad Iswandi Haris, Muhammad Iskandar Hilmiati Hilmiati, Hilmiati Himayanti, Yusfina Firia Huzro, Widia Khaeratun Imam Syahputra Yamin Kurniawan, Ahmad Aldi Lalu Mariawan Alfarizi Maharani, B Firia Maharani, B Fitria Megasari, Wita Muhammad Rizal Januardi Muhammad, Mahadir Oktaviantika, Ega Puspita, Novia Dara Sapwan, Sapwan Sri Sofiati Umami Sri Sofiati Umami, Sri Sofiati Taibin, Ali Yanti, Zuliana Rahma Yazid, Yazman Ziana, Elma