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Detection and Development of Infestation Rate of Aphelenchoides besseyi on Various Rice Seed Varieties Nikmatul 'Azizah; Siwi Indarti; Ani Widiastuti; Y. Andi Trisyono
Jurnal Perlindungan Tanaman Indonesia Vol 23, No 2 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.45558

Abstract

Aphelenchoides besseyi is a seed borne nematode caused white tip disease. Infested rice seeds are beneficial inoculum source forA. besseyi widespread. The objective of this research was to determine the occurence and development of symptom level caused by A. besseyi in rice seed varieties. The research was done by detecting A. besseyi in rice seed using Hoshino and Togashi method and counting the nematode population in the seed; observing the incubation time and measuring disease incidence every week. The result revealed that A. besseyi was found in 16 of 17 varieties, i.e. R1 IR64, R2 IR64, R3 IR64, R1 Situbagendit, R2 Situbagendit, Inpari 33, R1 Memberamo, R2 Memberamo, R3 Memberamo, R1 Way Apo Buru, R2 Way Apo Buru, Mekongga, Pepe, Ketan, Sintanur, and Ciherang.A. besseyi infection increased every week in many varieties depend on quantitative (statistics) and qualitative (symptom) indicators. There were 4 varieties have to be investigate further because they were infested by A. besseyi>30 nematodes/100 seeds, i.e. R1 Way Apo Buru, R1 Situbagendit, Ketan and R3 IR 64. A practice that is also required is the elimination of A. besseyi inoculum in rice seed before cultivate.
Preference of the Waterlily Aphid, Rhopalosiphum nymphaeae (Hemiptera: Aphididae) on Four Hostplants Ilmam Zul Fahmi; Nugroho Susetya Putra; Siwi Indarti; Satoru Sato
Jurnal Perlindungan Tanaman Indonesia Vol 25, No 1 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.62855

Abstract

Waterlily aphid Rhopalosiphum nymphaeae is one of the aphids that have many hosts, including Azolla filiculoides, Limnobium laevigatum, Monochoria vaginalis and Spirodela polyrhiza. The aim of this study was to study the effect of host shift and confirm the results  of previous studies on the effect of nitrogen and carbon factors among A. filiculoides, L. laevigatum, M. vaginalis and S. polyrhiza on the level of aphid preference and number of offspring. Analysis of the nitrogen and carbon content of plants was also carried out to confirm the preference and number of offspring produced by aphids. The study began with maintaining aphids on the four tested hosts, up to the 4th generation. Twenty five individuals were randomly selected from each host, then released on the inner wall of the plastic container (14 x 7.5 x 15 cm3) which was filled with four hosts arranged side by side. Observations were made every 24 hours up to 97 hours starting from the first hour after treatment. Observations after 97 hours showed that waterlily aphids imago preferred L. laevigatum the most (49.28%), then on M. vaginalis (20.43%), S. polyrhiza (16.33%), and A. filiculoides (1.75%). Meanwhile, the number of offspring produced by each group of aphids that selected on four hosts were: 46.65 individuals on L. laevigatum, 37.8 individuals on M. vaginalis, 19 individuals on S. polyrhiza, and 0.6 individuals on A. filiculoides. The analysis showed that the highest nitrogen content was found in M. vaginalis (4.16%), followed by S. polyrhiza (3.71%), L. laevigatum (2.33%), and A. filiculoides (2.08%).
Identification of Pathogens Causing Bulb Rot Disease on Garlic (Allium sativum L.) in Central Java, Indonesia Lutfi Arifin; Siwi Indarti; Arif Wibowo
Jurnal Perlindungan Tanaman Indonesia Vol 25, No 1 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.64743

Abstract

Garlic bulb rot disease was found from garlics (Allium sativum L.) cultivated from 2017 to 2019 by farmers in Central Java Province, Indonesia. The initial symptoms of the disease were stunted, leaf yellowing, and necrotizing to rotten bulbs. This research was conducted to determine the major causal agent of garlic bulb rot disease in Central Java. A survey was carried out in five regencies across Central Java that were major garlic-producing areas. Nematodes were isolated using water immersion methode and pathogenic fungi were isolated on Potato Dextrose Agar (PDA). Nematode identification was carried out based on the Ditylenchus dipsaci morphological and morphometric character. Seven isolates of Fusarium species were obtained from infected garlic. Identification of four chosen isolates were performed by sequencing the TEF-1α gene. The TEF sequence of isolate TB3, KK1, and KK4 showed 99% similarity with several F. oxysporum, BT3 sequences showed 98% identity with several F. solani, and all were deposited in the NCBI GenBank. Three locations were positively infected by the interaction between D. dipsaci and Fusarium sp. Based on the results of the morphological identification, parasitic nematode was identified as D. dipsaci, while based on the morphological and molecular identification isolates Fusarium were identified as F. oxysporum and F. solani, respectively, as first report causal agents of garlic bulbs rot in Central Java.
EARLY STEPS OF TOMATO BREEDING RESIST TO ROOT-KNOT NEMATODE Rudi Hari Murti; Fardatun Muamiroh; Tata Rina Wahyu Pujiati; Siwi Indarti
AGRIVITA, Journal of Agricultural Science Vol 34, No 3 (2012)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v34i3.215

Abstract

The inheritance pattern of resistant tomato to root-knot nematode was studied. GM2 accession and Gondol Putih (GP) cultivars were used as resistant and susceptible parent, respectively. Parental plants, F1 generation, and the F2 generation were grown individually in the sterile soil. One thousand of larvae stage 2 (L2) nematode of M. incognita was infested in each plant. Data of root gall intensity, the number of egg mass, population of L2 nematodes in root and soil were analyzed with the Kormogorov-Smirnov’s test, Chi-square test, and potence ratio. The F2 selected resistant plants then were grown and self fertilized to identify of homozygote plants (F2) related to nematode resistant and good fruit characters. The result showed that the resistant to root-knot nematode was controlled by a dominant gene. Positive correlation between damage levels of roots, eggs mass number and L2 larvae population in the root was significant. Eleven selected plants, conferred the homozygous resistant gene, was prospective lines to be used pedigree or single seed descent selection in producing variety with resistant to nematode, high yield and quality of fruit.   Keywords: tomato, root-knot nematode, resistant gene, dominant, homozygous, correlation
Penapisan Pendahuluan Berbagai Aksesi Padi (Oryza sativa L.) Terhadap Patogen Tumbuhan Siwi Indarti; Taryono Taryono; Supriyanta Supriyanta; Ayu Suci Wulandari
Agrotechnology Innovation (Agrinova) Vol 2, No 2 (2019)
Publisher : Agrotechnology Innovation Center, Universitas Gadjah Mada, Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1011.888 KB) | DOI: 10.22146/agrinova.54706

Abstract

Kajian penapisan galur-galur padi terhadap serangan patogen tumbuhan merupakan tahapan penting untuk mendapatkan varietas padi unggul tahan penyakit. Tiga puluh empat galur padi dikaji penapisan untuk mengetahui tanggapannya terhadap serangan patogen tumbuhan. Penelitian dilakukan pada lahan persawahan irigasi yang merupakan daerah endemik patogen tersebut. Rancangan penelitian menggunakan Rancangan Acak Kelompok Lengkap (RAKL) dengan 3 blok sebagai ulangan. Pengamatan dilakukan terhadap kerusakan tanaman yang diakibatkan oleh berbagai jenis patogen tumbuhan meliputi bercak nekrotik kecoklatan daun yang diakibatkan oleh  jamur patogen Cercospora oryzae, penyakit blast berupa bercak daun yang berbentuk belah ketupat  di bagian tengah berwarna abu-abu yang diakibatkan oleh jamur patogen Pyricularia oryzae, busuk pelepah daun bendera yang diakibatkan oleh jamur patogen Sarocladium oryzae, bercak daun yang berukuruan sempit berwarna hijau gelap kekuningan sampai coklat yang diakibatkan oleh bakteri patogen Xanthomonas campestris, serta busuk pangkal batang yang disebabkan oleh jamur patogen Sclerotium oryzae. Hasil penelitian menunjukkan bahwa gejala serangan busuk pangkal batang ditemukan pada 34 galur padi Penyakit bercak daun  menyerang hampir semua galur padi, kecuali UGM-F-00005 dan UGM-F-00033. Serangan penyakit blast tidak ditemukan pada beberapa galur yaitu UGM-F-00016, UGM-F-00018, UGM-F-00019, UGM-F-00021, UGM-F-00029 dan UGM-F-33. Penyakit busuk pelepah daun ditemukan hampir semua galur, kecuali  galur UGM-F-00028 dan UGM-F-00029.
Penapisan Pendahuluan Berbagai Aksesi Padi (Oryza sativa L) Terhadap Serangan Hama Serangga dan Nematoda Parasit Tanaman Siwi Indarti; Taryono Taryono; Supriyanta Supriyanta; Ayu Suci Wulandari
Agrotechnology Innovation (Agrinova) Vol 2, No 2 (2019)
Publisher : Agrotechnology Innovation Center, Universitas Gadjah Mada, Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2144.661 KB) | DOI: 10.22146/agrinova.54716

Abstract

Kajian penapisan padi terhadap serangan organisma pengganggu tanaman merupakan tahapan penting untuk mendapatkan varietas unggul. Tiga puluh empat aksesi padi dilakukan penapisan untuk mengetahui tanggapannya terhadap serangan hama serangga dan nematoda parasit tanaman. Penelitian dilakukan pada lahan persawahan irigasi yang merupakan daerah endemik kedua golongan hama tersebut. Penelitian menggunakan Rancangan Acak Kelompok (RAK) dengan 3 blok sebagai ulangan. Pengamatan berupa gejala kerusakan tanaman yang diakibatkan oleh berbagai jenis hama serangga dan nematoda parasit tanaman. Hasil penelitian menunjukkan serangan hama walang sangit  pada semua aksesi padi yang diuji. Serangan wereng hijau ditemukan pada aksesi no UGM-F-00004 dan UGM-F-00008, sedangkan serangan penggerek batang hanya ditemukan pada aksesi nomor UGM-F-00005 dan UGM-F-00006. Jenis nematoda parasit tanaman yang ditemukan adalah Meloidogyne graminicola, Hirsmaniella oryzae, Helicotylenchus sp., dan Criconemoides sp. dengan tingkat populasi yang beragam. Aksesi nomor UGM-F-00028 paling rentan terhadap serangan nematoda M.graminicola. dengan populasi tertinggi > 600 nematoda per 100 g tanah dan 900 nematoda per 5 g akar.
SKRINING KEMAMPUAN PATOGENISITAS ISOLAT ACTINOMYCETES ASAL RHIZOSFER KOPI DALAM MENGINFEKSI TELUR DAN LARVA STADIUM 2 NEMATODA PURU AKAR Meloidogyne Sp. Agustinur Agustinur; Siwi Indarti; Donny Widianto
Jurnal Agrotek Lestari Vol 3, No 2 (2017): Oktober
Publisher : Universitas Teuku Umar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (80.056 KB) | DOI: 10.35308/jal.v3i2.618

Abstract

Root-knot Nematode (Meloidogyne sp.) is one of plant-parasitic nematode causing a serious damage of coffee. The spread of this parasites occurs through the distribution the number of eggs on the plantation, while the process of infection in plants is acted by juvenile 2. Therefore, to solve this problem, several effort to decrease the infesting nematodes are needed. This may be done by using natural enemy that can suppress and control eggs and juvenile 2. The objective of this research was to determine the potency of pathogenicity of actinomycetes toward Meloidogyne eggs and juvenile 2. A total of 39 actinomycetes isolates were obtained from rhizosphere soil of coffee plantation from PT Perkebunan Nusantara XII, Bondowoso, East Java. The result of in vitro pathogencity screening showed that there are 5 isolates (P2A01, K1B1, P4A04, H106 and H203) than had the potency in controlling eggs and juvenile 2. Then, crude extract of the isolates tested on mature and immature eggs. The result showed that isolates H106 and P2A01 were able to control Meloidogyne by triggering immature egg hatching and increase juvenile mortality. While isolates P4A04, K1B1 and H203 could decrease the percentage of egg hatching as well as increase juvenile mortality. Keywords: Actinomycetes, Meloidogyne sp., Pathogenicity, Egg, Juvenile 2
DIAGNOSIS STRUKTUR KOMUNITAS NEMATODA DI LINGKUNGAN RHIZOSFER GULMA SIAM (Chromolaena odorata) (L) R.M. KING AND H.ROBINSON Aji Suhandy; Tien Aminatun; Siwi Indarti
Kingdom (The Journal of Biological Studies) Vol 6, No 3 (2017): Jurnal Prodi Biologi Vol 6 No 3 Tahun 2017
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/kingdom.v6i3.6812

Abstract

Tujuan penelitian ini adalah (1) Mengetahui nematoda pada rhizosfer gulma siam dan (2) Mengetahui struktur komunitas nematoda yang terdapat dalam rhizosfer tanaman gulma siam dan mengetahui apakah lingkungan rhizosfer gulma siam merupakan lingkungan yang cocok untuk kehidupan nematoda. Penelitian ini dilaksanakan pada bulan Juni – Desember 2016. Pengambilan sampel tanah dan akar Chromolaena odorata dilakukan di lahan karst, lahan pantai berpasir dan lahan vulkanik. Pengambilan sampel tanah dilakukan pada lima titik pada tiap lokasi sampling. Pencuplikan sampel tanah dilakukan dengan mengambil tanah utuh berbentuk persegi pada area sekitar perakaran gulma siam. Hasilnya ditemukan 55 genus nematoda yang berasal dari 20 famili. Nilai indeks keanekaragaman sedang, yaitu 1,82 pada rhizosfer gulma siam di lahan karst, 2,91 di lahan pantai berpasir dan 3,06 di lahan vulkanik. Rhizosfer gulma siam merupakan ekosistem yang sesuai untuk kehidupan nematoda. Struktur komunitas nematoda yang berada pada rhizosfer gulma siam merupakan struktur komunitas yang cenderung stabil. Kata kunci : Gulma siam (Chromolaena odorata), Rhizosfer, Komunitas nematoda
Resistance of Spodoptera exigua Population from Nganjuk against Methomyl, Chlorfenapyr, and Emamectin Benzoate Aziz, Abdul; Martono, Edhi; Indarti, Siwi; Trisyono, Y. Andi
Jurnal Perlindungan Tanaman Indonesia Vol 27, No 2 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.83646

Abstract

Spodoptera exigua Hubner (Lepidoptera: Noctuidae) is the main pest of shallot causing significant economic losses. The continuous use of insecticides has resulted S. exigua to develop resistant against them. This study aims to determine the level of S. exigua resistance of Nganjuk and Bantul populations against methomyl, chlorfenapyr, and emamectin benzoate and temporal resistance of S. exigua of Nganjuk population to methomyl. Nganjuk population were sampled in June, September, and December. First instar larvae from the first generation were used tested using the leaf-dipping method. Temporal resistance test was carried out using the same concentrations. The resistance ratios of Nganjuk population to the insecticides methomyl, chlorfenapyr, and emamectin benzoate were 58.8; 8.8; and 2.5 fold respectively. The LC50 values of S. exigua of Nganjuk population collected in June, September, and December were 1127.44; 50.62; and 366.76 mg [AI] liter-1, respectively. The results of this study indicated that the S. exigua of Nganjuk population was highly resistant to methomyl and its level has changed over time. Resistance management by rotating the type of insecticide is not sufficient but should also consider its change of resistance pattern over time.
Exploration of Nematophagous Fungi from Coffee Rhizosphere Soil and their Potential as Biological Control Agents against Root-lesion and Root-knot Nematodes Indarti, Siwi; Aldina, Rinda Fajrin; Widianto, Donny; Prijambada, Irfan Dwidya; Maharani, Rina; Kurniasari, Irianti; Waele, Dirk De
Jurnal Perlindungan Tanaman Indonesia Vol 29, No 2 (2025): In Progress
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.102469

Abstract

Root-lesion and root-knot nematodes are amongst the most important pathogens of coffee and can cause substantial yield losses and quality reductions. Environmental and health concerns concerning the use of chemical pesticides have increased the need for alternative management strategies against plant-parasitic nematodes. The aim of our study was to isolate and identify nematophagous fungi from nematode-infested coffee production areas and evaluate their potential as biocontrol agents. Our study was carried out in two stages: 1) fungi isolation and evaluation of their ability to affect eggs or vermiform developmental stages of root-lesion and root-knot nematodes; 2) identification of fungal isolates to species level. Eleven fungal isolates were able to affect either the nematode eggs or the vermiform developmental stages. The ability of these fungi to produce extracellular enzymes were also evaluated. This study highlights Indonesian nematode infected coffee rhizosphere soils as a rich source of nematophagous fungi, with eleven isolates showing promises to be used for integrated pest management strategies. Future work should assess field efficacy under local conditions while monitoring impacts on soil food webs and non-target organisms.