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All Journal Journal of the Indonesian Tropical Animal Agriculture Tropical Animal Science Journal Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences) Bantara Journal of Animal Science Jurnal Agrisistem Tropical Animal Science Livestock and Animal Research Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Journal of Smart Agriculture and Environmental Technology
Lilik Krismiyanto
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Energy Environmental Science Immunology & microbiology Social Sciences Veterinary

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Effect of Adding Palm Kernel Meal Extract to Rations Using Microparticle Protein Sources On Fatty Meat and Carcass Weight of Broiler Chicken Krismiyanto, Lilik; Yunianto, Vitus Dwi Yunianto Dwi; Suthama, Nyoman; Amanusa, Agritio
Bantara Journal of Animal Science Vol. 5 No. 1 (2023)
Publisher : Universitas Veteran Bangun Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32585/bjas.v5i1.3661

Abstract

The purpose of the study was to examine the effect of the addition of palm kernel meal extract (PKME) to rations using microparticle protein sources on fatty meat and carcass weight of broiler chickens. The experimental chickens used were unsexed CP707 strain broilers aged 8 days as many as 200 birds with an average body weight of 153.98 ± 4.41 g. Palm kernel meal extract as treatment material. Ration composition includes ground corn, rice bran, microparticle soybean meal, microparticle fish meal, CaCO3, premix, lysine and methionine. The study was arranged using a completely randomized design with 5 treatments and 4 replicates, each experimental unit was filled with 10 animals. The treatments applied are: T0=rations using protein microparticles/RPM, T1=RPM+PKME 0,2%, T2=RK+PKME 0,4%, T3=PKME+EBS 0,6% dan T4=PKME+EBS 0,8%. Parameters measured included fat digestibility, relative weight of abdominal fat, meat fat mass and carcass weight. Data were analyzed for variance at the 5% significance level, if there was a significant effect, Duncan's test was conducted at the 5% significance level. The results showed that the addition of EBS to rations using microparticle protein sources had a significant effect (p<0.05) on fat digestibility, relative weight of abdominal fat, meat fat mass and carcass weight of broiler chickens. The conclusion is that the addition of 0.8% palm kernel meal extract to rations using microparticle protein sources can reduce fat digestibility, relative weight of abdominal fat, meat fat mass and carcass weight of broiler chickens.
Addition of acidifier with inulin and papain enzyme in the ration towards leukocyte differential in bottle growth period I, Marhaeni; Yunianto, V. D.; Krismiyanto, L.
Journal of Smart Agriculture and Environmental Technology Vol. 1 No. 1 (2023): April 2023: First issued, published, 2023-04-26
Publisher : Indonesian Soil Science Society of South Sumatra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.60105/josaet.2023.1.1.22-25

Abstract

The aim of the study was to examine the effect of adding acidifier with inulin and papain enzymes in the ration on the differential leukocytes in turkeys during the growth period. The research material consisted of 80 male turkeys aged 12 weeks with an average weight of 1165 ± 62.24 g, an acidifier consisting of a mixture of lactic acid, propionic acid, and formic acid, and inulin sourced from dahlia tuber extract and the enzyme papain. The feed ingredients used in this study were corn, soybean meal, fish meal, rice bran, premix, CaCO3, lysine, and methionine. The research design used a completely randomized design with 5 treatments and 4 replications. The treatments applied included T0 (control diet/RK), T1 (RK + 1% acidifier), T2 (RK + 1% acidifier + 1.2% inulin), T3 (RK + 1% acidifier + 0.5% papain enzyme), and T4 (RK + 1% acidifier + 1.2% inulin + 0.5% papain enzyme). Parameters measured included heterophiles, lymphocytes, leukocytes, and total plasma protein. Data were analyzed using variance at the 5% level and Duncan's test of significant differences at the 5% level. The results showed that the addition of acidifier with inulin and papain enzymes had a significant effect (p<0.05) on the differential leukocytes (heterophiles, lymphocytes, leukocytes, and total plasma protein). The conclusion is that the addition of 1% acidifier + 1.2% inulin + 0.5% papain enzyme (T4) can maintain the health condition of the body, as observed from the differential leukocytes in male turkeys during the growth period.
Feeding dietary inclusion of inulin on immune status, protein metabolism, and growth performance of Kedu chicken Suthama, N.; Mangisah, I.; Krismiyanto, L.; Yunianto, V. D.; Mulyono, M.
Journal of the Indonesian Tropical Animal Agriculture Vol 50, No 1 (2025): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.50.1.22-32

Abstract

Indonesian Indigenous poultry originated from the central part of Java Island, called Kedu chick-en, given diet added with inulin of dahlia tuber extract (IDTE) to evaluate body resistance and protein deposition. The experimental animals were 150 birds of 14-day-old Kedu chicken (initial body weight was 148 ± 16.4 g) until 12 weeks old. Dietary treatments were T1: farmer’s diet with protein concen-trate; T2: T1 + 1.2% inulin of IDTE, and T3: modified diet with common protein sources + 1.2% IDTE. A completely randomized design was assigned with 3 treatments (5 replications with 10 birds each). Parameters observed were rate of passage, protein and fiber digestibilites, Nitrogen (N) and Cal-cium (Ca) retentions, body resistance (plasma concentration of corticosterone/CTC, level of immuno-globulin A/IgA, and heterophyl-lymphocyte/H/L ratio), protein metabolism (fecal Nτ–methylhistidine/Nτ–MH and muscle protein mass), feeds consumption, feed conversion ratio (FCR), and body weight gain (BWG). Analysis of variance and Duncan’s test were applied to analyze data. Rate of passage, N and Ca retentions, BWG, IgA level, and muscle protein mass increased; in contrast, FCR, fecal Nτ–MH, and plasma CTC decreased due to the feeding effect of the T3 diet. All parameters, except IgA level, between treatment T1 and T2 indicated similar values. It can be concluded that a modified diet added with IDTE (T3) can modulate the increase in N retention and immune status, with the decrease in muscle protein degradation indicated by low fecal Nτ–MH excretion, and finally, brings about a bet-ter growth rate, with higher muscle protein deposition.
Improving broiler growth and immunity with encapsulated Cosmos caudatus and Andrographis paniculata extracts at high stocking density Agusetyaningsih, I.; Krismiyanto, L.; Yudiarti, T.; Widiastuti, E.; Sari, D. A. P.; Pawesti, S. D.; Sugiharto, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 50, No 2 (2025): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.50.2.111-121

Abstract

The community's growing demand for chicken meat as an animal protein source has presented farmers with the challenge to alter the care of broiler chickens by using high cage density. The aim of this study is to investigate the effect of diet supplementation with encapsulated extract of kenikir (Cosmos caudatus) and bitter (Andrographis paniculata) extracts on broiler diet to growth perfor-mance, immunity status, and carcass proportion. A total of 370 day-old Cobb broiler chicks, weighing 45.25 ± 0.89 g, were divided into five groups: T0: control, basal diet + density 10 chicks/m2, T1: basal diet + density 16 chicks/m2, T2: basal diet + density 16 chicks/m2 + Cosmos caudatus 1 g/kg feed, T3: basal feed + density 16 chicks/m2 + Andrographis paniculata 1 g/kg feed, T4: basal feed + density 16 chicks/m2 + Cosmos caudatus 0.5 g/kg feed + Andrographis paniculata 0.5 g/kg feed). At the end of the study, the blood sample and internal organ were collected to further analysis. The final weights of groups T2, T3, and T4 were better than T0 and T1, while T1 has the lowest weight among the other groups. Chicks in T0, T2, T3 and T4 had lower PDW levels compared to T1. Chicks in T4 have a high-er jejunum villi height compared to T0, T1, T2 and T3 groups. The treatments did not affect the small intestine and giblets relative organ wights (P>0.05). The group of T0, T2, T3, and T4 have a fewer duo-denum lesion compared to T1. The group of T2, T3, and T4 have a better structure in jejunum cells compared to T0 and T1. Lesion score of Bursa Fabricius and spleen were greater in T3 and T4 com-pared to T0, T1 and T2 groups. And T1 have the lowest ND antibody compared to other groups. In con-clusion, giving encapsulation of kenikir (Cosmos caudatus) and bitter (Andrographis paniculata) ex-tracts on broiler diet can improve production performance and immune status of broiler chickens.
Intestinal Morphology, Protein Digestibility, and Broiler Performance Fed Encapsulated Dahlia Tuber Extract and Bacillus subtilis Anjani, L.; Yunianto, V. D.; Suthama, N.; Krismiyanto, L.
Tropical Animal Science Journal Vol. 48 No. 4 (2025): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2025.48.4.338

Abstract

This study aimed to evaluate the effects of encapsulated dahlia tuber extract and Bacillus subtilis (EdteBs) on intestinal morphology, protein digestibility, and broiler performance. A total of 200 eight-day-old Ross 308 broilers (initial body weight: 194.60 ± 3.46 g) were divided into four dietary treatments with five replications. The treatments were: T0 – basal diet (control) without EdteBs supplementation; T1 – T0 + 0.1% EdteBs; T2 – T0 + 0.2% EdteBs; and T3 – T0 + 0.3% EdteBs. Variables measured included potential hydrogen (pH), lactic acid bacteria (LAB) population, coliform count, villus height, crypt depth, intestinal segment weight and height, protein digestibility, feed intake, body weight gain (BWG), feed conversion ratio (FCR), and income over feed cost (IOFC). Data were analyzed using analysis of variance, followed by Duncan’s test at a 5% significance level (p<0.05). Results showed that EdteBs supplementation at higher levels (T2 and T3) significantly (p<0.05) increased LAB population, villus height, crypt depth, and intestinal segment weight and height, while pH and coliform counts decreased. Protein digestibility and BWG also significantly improved (p<0.05) in T2 and T3. Both feed intake and BWG increased, particularly in T3, contributing to lower FCR and higher IOFC. In conclusion, EdteBs supplementation enhances intestinal morphology, protein digestibility, and broiler performance, with the most effective level at 0.3%.
The Effect of Phytobiotics and Lactobacillus acidophilus of The Diet on Protein Digestibility, Egg Calcium and Protein Mass and Egg Content Weight of Laying Hens Yunianto , Vitus Dwi; Yuniasari, Finas Anggi; Suthama, Nyoman; Krismiyanto, Lilik
Journal of Smart Agriculture and Environmental Technology Vol. 3 No. 2 (2025): August 2025, Published 2025-08-19
Publisher : Indonesian Soil Science Society of South Sumatra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.60105/josaet.2025.3.2.57-64

Abstract

The study aimed to assess the feeding of a mixture of onion peel flour, garlic peel, bay leaves and Lactobacillus acidophilus on crude protein digestibility, calcium mass and egg protein and egg weight in laying hens. The livestock used were 72-week-old Isa Brown strain laying hens totaling 144 birds with an average body weight of 1824 ± 126.15 g. The study was arranged in a completely randomized design (CRD) with 6 treatments and 4 replicates, each experimental unit consisted of 6 birds. The treatment given is T0 = basal diet, T1 = basal diet + 1.2 ml Lactobacillus acidophilus, T2 = basal diet + phytobiotics 2%, T3 = basal diet + phytobiotics 2% + 1.2 ml Lactobacillus acidophilus, T4 = basal diet + phytobiotics 4% + 1.2 ml Lactobacillus acidophilus, T5 = basal diet + phytobiotics 6% + 1.2 ml Lactobacillus acidophilus. Parameters observed included protein digestibility, egg protein and calcium mass, and egg content weight. Data were analyzed for variance, if the treatment had a significant effect (p<0.05) followed by Duncan’s test at the 5% significance level. The results showed that giving a mixture of onion peel flour, garlic flour, bay leaves and Lactobacillus acidophilus had a significant effect (p<0.05) on protein digestibility, protein mass and egg calcium and egg weight in laying hens. The conclusion is that the addition of 2% phytobiotic plus 1.2 ml Lactobacillus achidophilus to the ration of laying hens can effectively increase protein digestibility, egg calcium mass and egg weight in laying hens.
Co-Authors Agritio Amanusa Agusetyaningsih, I. Amal Nur, Fauziah Amanusa, Agritio Anjani, L. Azki Azhari Azmi Bambang Sukamto Bambang Sukamto Bambang Sukamto Doni Eko Prabowo E. Widiastuti Emerson Umbu Hemur Patangara Erma Widiyanti Fajar Wahyono Fajar Wahyono H. I. Wahyuni H.I. Wahyuni I, Marhaeni I. Mangisah ISTNA MANGISAH Kevita Widya Ningsih M. Mulyono N. Suthama Nuraeni Nyoman Suthama Nyoman Suthama Pawesti, S. D. Prabowo, Doni Eko S. Sugiharto Sari, D. A. P. Soraya Faradila, Soraya Tristiarti Tristiarti Turrini Yudiarti V. D. Yunianto Vitus Dwi Yunianto Budi Ismadi Vitus Dwi Yunianto Dwi Yunianto Yunianto , Vitus Dwi Yunianto, Vitus Dwi Yunianto Dwi Yuniasari, Finas Anggi