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All Journal Nexus Pendidikan Kedokteran dan Kesehatan Nexus Kedokteran Komunitas Nexus Kedokteran Klinik Nexus Biomedika Proceeding Muhammadiyah International Public Health and Medicine Conference Gudang Jurnal Ilmu Kesehatan
Nur Hafidha Hikmayani
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Journal : Nexus Biomedika

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Pengaruh Pemberian Ekstrak Daun Sukun (Artocarpus altilis F.) terhadap Perbaikan Jumlah Sel-β Pankreas pada Tikus (Rattus norvegicus) Model Diabetes Melitus Hidayatulloh, Achmad Syarif; Suyatmi, .; Hikmayani, Nur Hafidha
Nexus Biomedika Vol 3, No 2 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: Diabetes mellitus is a metabolic disorder caused by decrease of insulin production and/or sensitivity. The hyperglycemia state increases free radical production which can damage pancreatic β cells through oxidative stress mechanism. Breadfruit leaves contain antioxidants such as flavonoids, phenolics and tannins which can neutralize free radical activity. This study aims to examine the effect of breadfruit leaf extract on pancreatic β cells in diabetic rats. Methods: This was an experimental study using posttest only group design. Subjects were 30 male white rats aged 2-3 months and weighed 160-200 g. Subjects were divided into 5 treatment groups. Normal group, treatment group I, II, and III were intraperitoneally induced by 65 mg/kgBW streptozotocine and 230 mg/kgBW nicotinamide. Negative control group and normal group were administered 2 ml/kgBW/day NaCMC, whereas treatment groups I, II, and III were administered breadfruit leaf extract at doses of 200 mg/kgBW/day, 400 mg/kgBW/day, and 800 mg/kgBW/day, respectively. Treatments were conducted for 14 days and rats were terminated at day 15th. The rats’ pancreases were made into preparations of glass slides with Gomori staining. Data on the numbers of normal pancreatic β cells were compared by the Kruskal Wallis test, followed by the Mann-Whitney U tests. Results: There was a significant difference of the numbers of normal pancreatic β cells among the groups (p<0.001). Results from Mann-Whitney U tests showed no significant differences, except comparisons between negative control group and treatment group I (p=0.548), and between treatment group III and normal group (p=0.548). Conclusion: Breadfruit leaf extract can improve the numbers of pancreatic β cells in diabetic rats with an optimal dose of 800 mg/kgBW/day. Keywords: Breadfruit leaf extract, pancreatic β cells, diabetes mellitus, STZ-NA. 
Pengaruh Pemberian Ekstrak Daun Sukun (Artocarpus altilis F.) terhadap Penurunan Kadar Malondialdehid (MDA) pada Tikus (Rattus norvegicus) Model Diabetes Melitus Gresmita Rindi Winarti; Ida Nurwati; Nur Hafidha Hikmayani
Nexus Biomedika Vol 3, No 1 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Background: Diabetes mellitus is a chronic metabolic disease that causes increased free radical activity in the body. Excessive free radicals contribute to diabetes complications. The free radicals will react with lipids and then they can form malondialdehyde. This free radicals can be neutralized by antioxidants. Breadfruit (Atrocarpus altilis F.) leaf contains flavonoids and phenolics which serve as antioxidants. This research aimed to determine the effect of breadfruit leaf extract on malondialdehyde levels in diabetic rats. Methods: This was an experimental research using posttest only control group design. Subjects were 30 male white rats, three months old with average body weight of 160-200 g. Subjects were randomly divided into five treatment groups: Normal group, negative control group, treatment group I, treatment group II, and treatment group III. All subjects of the groups, except normal group, were induced by 65 mg/kgBW streptozotocin (STZ) and 230 mg/kgBW nicotinamide (NA). Each subjects in normal and negative control group were administered 2 ml/kgBW/day Natrium Carboxymethyl Cellulose (Na-CMC), whereas treatment groups I, II, and III were administered breadfruit leaf extract at doses of 200 mg/kgBW/day, 400 mg/kgBW/day, and 800 mg/kgBW/day, respectively. The treatments were done for 14 days. Malondialdehyde levels were measured from blood samples collected via retro-orbital veins. Data were analyzed using Kruskal-Wallis test followed by Mann-Whitney U tests (a=0.05). Results: Kruskal-Wallis test showed a significant difference of MDA levels among five groups (p<0.001). Statistically significant differences of MDA levels were further found in all pairs of two-group comparisons, except between the negative control group and the treatment group I (p=0.690). Conclusion: Breadfruit leaf extract could decrease malondialdehyde levels in diabetic rats induced by STZ-NA. The dose of 800 mg/kgBW/day of breadfruit leaf extract showed the greatest effect. Keywords: Breadfruit leaf extract, MDA, Diabetes mellitus
Pengaruh Pemberian Ekstrak Daun Sukun (Artocarpus altilis F.) terhadap Perbaikan Jumlah Sel-? Pankreas pada Tikus (Rattus norvegicus) Model Diabetes Melitus Achmad Syarif Hidayatulloh; . Suyatmi; Nur Hafidha Hikmayani
Nexus Biomedika Vol 3, No 2 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: Diabetes mellitus is a metabolic disorder caused by decrease of insulin production and/or sensitivity. The hyperglycemia state increases free radical production which can damage pancreatic ? cells through oxidative stress mechanism. Breadfruit leaves contain antioxidants such as flavonoids, phenolics and tannins which can neutralize free radical activity. This study aims to examine the effect of breadfruit leaf extract on pancreatic ? cells in diabetic rats. Methods: This was an experimental study using posttest only group design. Subjects were 30 male white rats aged 2-3 months and weighed 160-200 g. Subjects were divided into 5 treatment groups. Normal group, treatment group I, II, and III were intraperitoneally induced by 65 mg/kgBW streptozotocine and 230 mg/kgBW nicotinamide. Negative control group and normal group were administered 2 ml/kgBW/day NaCMC, whereas treatment groups I, II, and III were administered breadfruit leaf extract at doses of 200 mg/kgBW/day, 400 mg/kgBW/day, and 800 mg/kgBW/day, respectively. Treatments were conducted for 14 days and rats were terminated at day 15th. The rats pancreases were made into preparations of glass slides with Gomori staining. Data on the numbers of normal pancreatic ? cells were compared by the Kruskal Wallis test, followed by the Mann-Whitney U tests. Results: There was a significant difference of the numbers of normal pancreatic ? cells among the groups (p<0.001). Results from Mann-Whitney U tests showed no significant differences, except comparisons between negative control group and treatment group I (p=0.548), and between treatment group III and normal group (p=0.548). Conclusion: Breadfruit leaf extract can improve the numbers of pancreatic ? cells in diabetic rats with an optimal dose of 800 mg/kgBW/day. Keywords: Breadfruit leaf extract, pancreatic ? cells, diabetes mellitus, STZ-NA.
Pengaruh Ekstrak Kulit Manggis dalam Mencegah Peningkatan Kadar Blood Urea Nitrogen pada Tikus Putih (Rattus norvegicus) Galur Wistar yang Diinduksi Gentamisin Putri Ayu Winiasih; Endang Sri Hardjanti; Nur Hafidha Hikmayani
Nexus Biomedika Vol 3, No 1 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Background: Gentamicin, which is often used for infection treatment against aerobic Gram negative bacteria, is known having nephrotoxic effects through the inflammation mechanisms. Mangosteen pericarp has antioxidant activity and antiinflammation properties which attenuates oxidative stress, inflammatory and fibrotic pathways, hence it potentially prevents renal damages. Gentamicin-induced renal damages can be measured from elevation of blood urea nitrogen (BUN) levels. The aim of this study was to evaluate the effect of mangosteen pericarp extract on BUN levels in Wistar rats induced by gentamicin. Methods: This was an experimental study with pre- and posttest controlled group design. Thirty white male Wistar rats aged 8-12 weeks and weighed 135-170 grams were randomly divided into three groups. Normal control group (P1) was administered orally with Natrium Carboxymethylcellulose (Na CMC) 0.5%. Negative control group (P2) got intraperitoneal (i.p.) injection of gentamicin at total dose of 100 mg/kgBW. Treatment group (P3) was induced by gentamicin and 125 mg/kgBW mangosteen pericarp extract. The extract was administered orally thirty minutes after gentamicin injection for ten consecutive days. Blood samples were collected from retroorbital veins and BUN levels were measured at day 0 and day 11. Data were analyzed statistically by one-way ANOVA followed by Tukeys multiple comparison tests (?=0.05). Results: One-way ANOVA test showed no significant difference in pretest BUN levels among three groups (p=0.902). At day 11, BUN level in P2 group was significantly higher than those in P1 and P3 groups. Tukeys multiple comparison test further showed significant difference between P1-P2 and P2-P3 (p<0.001). No significant difference of BUN level was found between P1-P3 (p=0.386). Conclusions: Mangosteen pericarp extract could significantly prevent the elevation of BUN levels in Wistar rats induced by gentamicin. Keywords: mangosteen pericarp extract, gentamicin, BUN
Efek Hepatoprotektif Sari Belimbing Manis Terhadap Kadar SGPT: Studi Awal Terhadap Tikus yang Diinduksi Parasetamol Dosis Toksik Hana Amatillah; Endang Ediningsih; Nur Hafidha Hikmayani
Nexus Biomedika Vol 2, No 3 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: Star fruit is a good source of natural antioxidants which can prevent oxidative damages and modulate reactive free-radicals. However, no study had been carried out so far on the hepatoprotective effect of star fruit. This research aimed to study the hepatoprotective effect of star fruit juice on SGPT level in paracetamol- intoxicated rats. Methods: This was a laboratory experimental study with posttest only controlled group design. A total of 30 white male, Wistar rats (Rattus norvegicus), aged 2-3 months and weighed 160-200 grams was assigned into five groups. Normal group and negative control group were administered distilled water, while positive control group was administered Curcuma (3.6 mg/200 g bw). The treatment group 1 and 2 were administered star fruit juice at concentration of 50% and 100%, successively. This study was performed for 14 days. In the last 3 days, all groups except normal group were induced by paracetamol at toxic dose (291.6 mg/200 g BW) an hour after the treatment of star fruit juice (for treatment group 1 and 2) was done. Blood samples were collected from retro-orbitalis venous to measure SGPT levels. The data were analyzed statistically using one-way ANOVA followed by LSD test. Results: The analysis of SGPT with one-way ANOVA showed a significant difference across all groups. Further, the LSD tests of SGPT level showed significant differences between negative control group (182.5 U/L 14.0) and the two star fruit groups (p values <0.001). SGPT mean in treatment group 1 (87.0 U/L 14.1) and treatment group 2 (91.7 U/L 14.9) also differed from those of positive control (67.1 U/L 5.5, p=0.008) and normal group (53.8 U/L 8.6, p=0.002). There was no significant difference between two groups treated with star fruit juice (p=0.511). Conclusions: Star fruit juice at concentration of 50% and 100% significantly inhibited the elevation of SGPT levels in paracetamol-intoxicated rats. Keywords: Star fruit juice, SGPT, paracetamol, rats
Pengaruh Pemberian Ekstrak Kulit Manggis (Garcinia mangostana, Linn.) dalam Mencegah Peningkatan Kadar Kreatinin Darah pada Tikus (Rattus norvegicus) yang Diinduksi Gentamisin Surya Adhi Prakoso; Endang Sri Hardjanti; Nur Hafidha Hikmayani
Nexus Biomedika Vol 3, No 1 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Background: Gentamicin may cause nephrotoxicity and inhibits blood creatinine excretion. Xanthone from mangosteen pericarp has antioxidant and antiinflammation effects and can potentially protect kidney from inflammatory-mediated gentamicins nephrotoxicity. Gentamicin-induced renal damage can be identified from the elevation of blood creatinine levels. The purpose of this research was to find the potential of mangosteen pericarp extract to prevent the elevation of blood creatinine levels. Methods: This was an experimental research with pre and posttest controlled group design. Samples were 30 male Wistar-strain rats, 2-3 months old with approximately 200 gram of weight. Samples were randomly divided into 3 groups:control group (KN) administered Na CMC 0.5% orally, negative control group (K-) administered gentamicin 100 mg/kgBW intraperitoneally, and treatment group (KD) administered gentamicin 100 mg/kgBW intraperitoneally and 125 mg/kgBW mangosteen pericarp extract orally. Blood creatinine levels were assessed before treatment and 10 days after treatment by spectrophotometer. Data collected were analysed by Kruskal-Wallis and Mann-Whitney tests. Results: There was significant difference of blood creatinine levels before and after treatment among the three groups (p<0.001). Mann-Whitney tests showed statistically significant differences of creatinine level between KN-K- and KD-K- (p<0.001). No significant difference of blood creatinine levels was found for KN-KD comparison(p=0.06). Conclusion: Mangosteen pericarp extract at dose of 125 mg/kgBW prevented the elevation of blood creatinine levels in Wistar rats induced by gentamicin. Keywords: mangosteen pericarp extract, gentamicin, blood creatinine level
Co-Authors . Suyatmi Achmad Syarif Hidayatulloh Afifa Naura Harahap Aris Sudiyanto Djoko Suwito Endang Ediningsih Endang Sri Hardjanti Endang Susilowati Ningsih Gresmita Rindi Winarti Hana Amatillah Ida Nurwati Lintang Mega Pertiwi Machmuroch . Mohammad Fanani Niken Ayu Pratiwi Putri Ayu Winiasih Sayekti Asih Nugraheni Sumardiyono Surya Adhi Prakoso