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ANALYSIS ON WHOLE BLOOD, SGOT, SGPT, AND TNF-a EXAMINATION IN PATIENTS WITH NON-DENGUE AND POSITIVE DENGUE FEVER (DF/DHF) Anggraini, Rahayu; Nasronudin, Nasronudin
Indonesian Journal of Tropical and Infectious Disease Vol 4, No 4 (2013)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (658.547 KB)

Background: In Indonesia has four serotypes, the DEN-1, DEN-2, DEN-3 and DEN-4. The management of Dengue virus becomes difficult because the patients were infected with different clinical profiles depending on the serotypes and genotypes of infecting dengue virus. Consequently, the diagnosis and treatment becomes difficult. Aim: The purpose of this study was to identify the difference between the results of laboratory tests between non-dengue fever and positive dengue fever. Method: This study was an observational cross-sectional study. Fifteen samples were diagnosed with dengue fever and fifteen samples with negative dengue fever on NS1, IgM / IgG-anti-DHF strip test results. Laboratory tests comprising whole blood, SGOT, SGPT, and TNFa were first examined when the patient came to the hospital. The collected data were analyzed by Chi-Square test SPSS version 13 for Windows. Result: The results of the study in two groups regarding sex, age, days of fever, grade, hemoglobin levels, leukocytes count, platelet count, hematocrit percentage, SGOT levels, and TNFa level were not significantly different with p > 0.050, whereas the SGPT level in non-dengue increased 3 x of normal value of 66.7%, n = 10/15 and in positive dengue fever the SGPT level was within normal limits, found in 60%, n = 9/15, so there was significant difference with p = 0.022 (p < 0.05). Conclusion: in non-dengue SGOT and SGPT levels increased of 1â3 times the normal value. In positive dengue fever SGOT levels increased 1â3 x normal value, but SGPT levels was within normal value, so SGPT levels can be used as a predictive factor for distinguishing the two types of fever.

KORELASI KADAR KOLESTEROL DENGAN KEJADIAN DIABETES MELLITUS TIPE 2 PADA LAKI-LAKI Anggraini, Rahayu
Jurnal Ilmu Kesehatan dan Kesehatan Vol 2 No 2 (2018): AUGUST
Publisher : UNUSA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/mhsj.v2i2.588

Abstract: Type 2 diabetes is one of the most common chronic diseases in the world. It is estimated thatmore than 140 million people worldwide are currently suffering from diabetes, and by 2025, anestimated more than 300 million people will suffer from the disease. The purpose of this study was toevaluate the correlation of cholesterol with the incidence of Diabetes Mellitus disease. The type of thisresearch is observational with cross-sectional analytic approach and done in PLN employees, total are112 people of which are mens. All participants underwent biochemical analysis of fasting blood sugar(BSN) and 2 hours PP (post prandial), and total cholesterol level test. Determination of glucose byglucose oxidase method and cholesterol level by CHOD-PAP method. The mean results in normalcholesterol (< 200 mg/dl) obtained BSN = 73 mg / dl, 2 Hours PP = 92 mg / dl, and in abnormalcholesterol (> 200 mg/dl) mean BSN = 81 mg / dl, 2 hours PP = 109 mg / dl, in independent T test withp = 0001 in both groups of different cholesterol levels. Results of the Pearson's correlation test, therewas a significant positive correlation between GDP and cholesterol levels (r = 0.262, p = 0.005), and 2hours PP and cholesterol levels (r = 0.258, p = 0.006). Conclusions: There was a significant positivecorrelation between 2 hours PP and cholesterol levels. This study shows there is a correlation ofcholesterol levels that develop diabetes mellitus type 2.

ANALISIS KADAR AKTIVITAS ANTIOKSIDAN, KADAR BESI, DAN pH PADA YOGURT SUSU KAMBING DENGAN PENAMBAHAN SARI KURMA (Phoenix dactylifera) Putri, Endah Budi Permana; Anggraini, Rahayu
Jurnal Teknologi Pangan dan Gizi Vol 20, No 1 (2021)
Publisher : Widya Mandala Surabaya Catholic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jtpg.v20i1.2834

Pencegahan virus COVID-19 dapat dilakukan dengan meningkatkan imunitas tubuh dengan mengkonsumsi pangan fungsional salah satunya yaitu yogurt. Susu kambing memiliki rasa yang kurang disukai sehingga dengan diolah menjadi yogurt dan penambahan sari kurma dapat lebih disukai. Di sisi lain, susu kambing dan kurma memiliki kandungan besi yang tinggi. Bahan yang digunakan yaitu susu kambing segar, bakteri asam laktat, kurma ajwa, dan sari kurma. Metode yang digunakan untuk menganalisis kadar aktivitas antioksidan adalah IC50 (Inhibition Concentration 50 Value), kadar besi (Fe) yaitu spektrofotometer UV â€“ Vis, dan nilai pH menggunakan PH-meter. Hasil penelitian menunjukkan bahwa yogurt susu kambing dengan penambahan sari kurma memiliki kandungan antioksidan yang sangat lemah berdasarkan metode analisis antioksidan IC50. Ada perbedaan signifikan kadar besi pada yogurt susu kambing dengan penambahan sari kurma ajwa dengan nilai paling tinggi (5,7827 mg/100g) bila dibandingkan dengan yogurt susu kambing dengan penambahan sari kurma komersial (5,2351 mg/100g), yogurt susu kambing (4,4348 mg/100g) dan yogurt susu sapi (3,6839 mg/100g). Terdapat perbedaan nilai pH pada yogurt susu sapi dan yogurt susu kambing namun perbedaan tidak berbeda nyata pada yogurt dengan penambahan sari kurma ajwa dan komersial.

CORRELATION OF TOTAL KOLESTEROL WITH DIABETES MELLITUS TYPE 2 IN MEN Rahayu Anggraini
TEKNOLOGI MEDIS DAN JURNAL KESEHATAN UMUM Vol 2 No 2 (2018): Medical Technology and Public Health Journal September 2018
Publisher : UNUSA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/mtphj.v2i2.572

The purpose of this study was to evaluate the correlation of total cholesterol with the incidenceof Diabetes Mellitus disease. The type of this research is observational with cross-sectional analyticapproach and done to PLN employees, total are 112 people of which are men. All participantsunderwent biochemical analysis of fasting blood sugar (BSN) and 2 hours PP (Post Prandial), andtotal cholesterol level test. Determination of glucose by glucose oxidase method and cholesterol levelby CHOD-PAP method. The mean results obtained GDP = 73 mg / dl, 2 Hours PP = 92 mg / dl innormal cholesterol patients, and in abnormal patients Cholesterol mean GDP = 81 mg / dl, 2 HoursPP = 109 mg / dl with p = 0000 in both groups of different cholesterol. Results of the Pearson'scorrelation test, there was a significant positive correlation between GDP levels and total cholesterol(r = 0.262, p = 0.005), and 2 hours PP and total cholesterol (r = 0.258, p = 0.006). Conclusions:There was a significant positive correlation between GDP levels, 2 hours PP, and total serumcholesterol. This study shows there is a correlation of total cholesterol levels that developdyslipidemia until diabetes mellitus type 2.

PENGARUH NORMAL FLORA Streptococcus sp. KARANG GIGI TERHADAP PEMERIKSAAN DARAH LENGKAP PADA MAHASISWA UNIVERSITAS NAHDLATUL ULAMA SURABAYA Rahayu Anggraini; Umi Hanik; Gilang Nugraha; Dwi Lestari Pertiwi
TEKNOLOGI MEDIS DAN JURNAL KESEHATAN UMUM Vol 1 No 1 (2017): Medical Technology and Public Health Journal March 2017
Publisher : UNUSA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/mtphj.v1i1.757

This study aims at determining the normal flora of Streptococcus sp., the inhabitants of the oral cavity in the tartar that can lead to health problems. This study is an observational study with cross sectional analytic approach performed on 47 samples of dental smear for Streptococcus sp. bacteria identification and EDTA blood samples for Hemoglobin level examination, hematocrit percent, the number of erythrocytes, leukocytes and platelets in University of Nahdlatul Ulama Surabaya students. The result of Independent Samples T Test data analysis in a cut point showed that the levels of hemoglobin, number of leukocytes and platelets look significantly different with p <0.05in individuals with normal flora Streptococcus alpha and Streptococcus gamma. The conclusion in this study, in individuals with normal flora Streptococcus alpha, can decrease hemoglobin level, whereas in individuals with normal flora Streptococcus gamma can increase the number of leukocytes and decrease the number of platelets.

KORELASI KADAR KOLESTEROL DENGAN KEJADIAN DIABETES MELLITUS TIPE 2 PADA LAKI-LAKI Rahayu Anggraini
Jurnal Ilmu Kesehatan dan Kesehatan Vol 2 No 2 (2018): AUGUST
Publisher : UNUSA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/mhsj.v2i2.588

Abstract: Type 2 diabetes is one of the most common chronic diseases in the world. It is estimated thatmore than 140 million people worldwide are currently suffering from diabetes, and by 2025, anestimated more than 300 million people will suffer from the disease. The purpose of this study was toevaluate the correlation of cholesterol with the incidence of Diabetes Mellitus disease. The type of thisresearch is observational with cross-sectional analytic approach and done in PLN employees, total are112 people of which are mens. All participants underwent biochemical analysis of fasting blood sugar(BSN) and 2 hours PP (post prandial), and total cholesterol level test. Determination of glucose byglucose oxidase method and cholesterol level by CHOD-PAP method. The mean results in normalcholesterol (< 200 mg/dl) obtained BSN = 73 mg / dl, 2 Hours PP = 92 mg / dl, and in abnormalcholesterol (> 200 mg/dl) mean BSN = 81 mg / dl, 2 hours PP = 109 mg / dl, in independent T test withp = 0001 in both groups of different cholesterol levels. Results of the Pearson's correlation test, therewas a significant positive correlation between GDP and cholesterol levels (r = 0.262, p = 0.005), and 2hours PP and cholesterol levels (r = 0.258, p = 0.006). Conclusions: There was a significant positivecorrelation between 2 hours PP and cholesterol levels. This study shows there is a correlation ofcholesterol levels that develop diabetes mellitus type 2.

ANTIGEN OMP (OUTER MEMBRANE PROTEIN) salmonella typhi FAGA LOKAL YANG IMUNODOMINAN DAN SPESIFIK TERHADAP ANTIBODI PENDERITA DEMAM TIFOID J. Nugraha; Rahayu Anggraini
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 13, No 3 (2007)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v13i3.912

Background of this research is that diagnostics of typhoid fever is still a health problem. Widal test, which is the mostly used test in Indonesia, frequently gives false positive results and is not reliable in endemic areas. On the other hand, the other confirmation test, bloodculture, is not sensitive and often give false negative results. OMP (Outer Membrane Protein) is known as a specific part of Salmonellatyphi and fragments of OMP still exist in the patient’s body since early infection until 2–3 weeks thereafter. In this study parts of OMPwhich react specifically with sera of typhoid fever in Indonesia were searched. These specific parts will then be developed as a diagnostickit for typhoid fever. Using Western Blot method, part of OMP will be searched, which is specifically react with sera of typhoid feverpatients in Indonesia. OMP derived from local phage type isolated in Indonesia was used. This OMP was separated with SDS-PAGE 12%and incubated with pooled sera of typhoid fever patients, and sera of control group, that is from Dengue haemorrhagic fever patientsand urinary tract infection with E. coli. Extraction of OMP was done by the method of Matsuyama. Contrary, this research failed to find a particular part of OMP which react specifically with sera of typhoid fever patients. There are certain parts of OMP which react also with sera of DHF & urinary tract infection patients. Our finding was different with the results from Malaysia, where it is reported thatantigen OMP 52 kD react specifically there. In order to develop a diagnostic tool for typhoid fever, we should consider another possiblespecific antigen other rather than using OMP.

PENGARUH JENIS LEUKOSIT DAN JUMLAH TROMBOSIT DENGAN KEJADIAN TOKSOPLASMOSIS TERHADAP PEKERJA DI LINGKUNGAN HEWAN Dinda Fadlliyah Dzikriana; Nur Kholifatul Hi’mah; Rahayu Anggraini
PROSIDING NATIONAL CONFERENCE FOR UMMAH Vol. 1 No. 1 (2020): Prosiding National Conference for Ummah (NCU) 2020
Publisher : Universitas Nahdlatul Ulama Surabaya

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The incidence of toxoplasmosis is increasingly spread throughout the world include Indonesia. An estimated 15-85% of the world is infected with toxoplasmosis, a disease caused by Toxoplasma gondii and transmitted from animals to humans. Toxoplasmosis in immunocompetent people produces long-term immunity, whereas in immunocompromises cause pathological disorders to death. Immunocompetent cells consist of 5 types of leukocytes that are used as indicators of immune quality. Platelets also have a role as the immune system against pathogens and are toxic to T. gondii takizoit. This study was conducted with the aim to determine the effect of leukocytes and platelet count with the incidence of toxoplasmosis on workers in the animal environment with using 30 samples from workers at the Bratang Bird Market in Surabaya. Serological examination of Toxoplasma gondii IgG/IgM using the Rapid Immunochromatographic (ICT) test method, with the results of IgG(-)/IgM(-) as much as 13.3% and IgG(+)/IgM(-) as much as 86.7%. Whereas examination of leukocyte types and platelet count using Sysmex XP-300 type 3-part diff hematology analyzer with an average neutrophil value of 59% (normal), lymphocytes 31% (normal), and mixed (eosinophils, basophils, monocytes) 10% (increase). The results of the Spearman correlation test are that there is no influence between the type of leukocytes and the number of platelets with the incidence of toxoplasmosis on workers in the animal environment, because the p-value > 0.05 which not significant.

Penerapan Kamar Sehat untuk Mengurangi Kejadian Skabies di PP. Al Fitrah As Salafiyah Surabaya Putri, Winawati Eka; Meidyta Sinantryana W; Rahayu Anggraini; Esty Puji Rahayu; Yuriske Agnovianto
PROSIDING SEMINAR NASIONAL PENGABDIAN KEPADA MASYARAKAT Vol. 3 No. 1 (2023): Prosiding Seminar Nasional Pengabdian Kepada Masyarakat : MEMAKSIMALKAN POTENSI
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/snpm.v3i1.1255

Latar belakang, Pondok pesantren (ponpes) adalah tempat berkumpulnya banyak orang dimana rentan terjadi penularan penyakit. Penyakit kulit banyak dijumpai di Indonesia seperti penyakit scabies yang disebabkan oleh parasit. Penyakit scabies mengakibatkan kerusakan pada kulit akibat infeksi sekunder dan gangguan kenyamanan akibat rasa gatal. Faktor yang berperan pada tingginya prevalensi penyakit scabies di negara berkembang terkait rendahnya tingkat kebersihan, akses air yang sulit, tingginya kepadatan hunian seperti penjara, pondok pesantren dan panti asuhan. Penyakit scabies ini kurang diperhatikan oleh santri di pondok pesantren, faktor penyebabnya adalah lingkungan yang kurang baik, personal hygiene yang buruk, pengetahuan yang kurang sehingga terjadinya risiko penularan penyakit scabies. Lingkungan merupakan faktor yang paling besar pengaruhnya terhadap derajat kesehatan masyarakat khususnya di lingkungan pondok pesantren, sehingga hal ini menjadi prioritas yang perlu diperhatikan dan dibenahi. Salah satunya caranya adalah dengan menjaga kebersihan kamar sehingga dapat menjadi kamar yang sehat untuk santri. Sehingga perlu dilakukan upaya edukasi dengan tujuan meningkatkan pengetahuan santri untuk dapat menerapkan kamar sehat di lingkungan pondok pesantren. Tujuannya adalah untuk Meningkatkan pemahaman dengan memberikan edukasi dan penyuluhan kepada santri husada agar dapat menerapkan kamar sehat untuk mengurangi kejadian Skabies di PP. Al Fitrah As Salafiyah Surabaya. Metode, Metode yang digunakan dalam penyuluhan adalah ceramah dan tanya jawab. Dilanjutkan dengan monitoring dan evaluasi secara berkala. Hasil, Santri dan santriwati PP. Al Fitrah sangat berantusias dalam mengikuti rangkaian acara penyuluhan ini. Dari penyuluhan kesehatan ini, para santri dapat menerapkan kamar sehat pada kamar masing-masing santri. Selain itu, santri juga bertambah pengetahuannya tentang penyakit skabies dan penularannya. Sehingga, santri dapat menjaga pola hidup sehat dan dapat terhindar dari penularan penyakit skabies. Kesimpulan, Penerapan Kamar Sehat untuk mengurangi kejadian Skabies di PP. Al Fitrah As Salafiyah Surabaya telah menambah pengetahuan dan pemahaman terhadap penerapan kamar sehat.

ANALYSIS ON WHOLE BLOOD, SGOT, SGPT, AND TNF-a EXAMINATION IN PATIENTS WITH NON-DENGUE AND POSITIVE DENGUE FEVER (DF/DHF) Anggraini, Rahayu; Nasronudin, Nasronudin
Indonesian Journal of Tropical and Infectious Disease Vol. 4 No. 4 (2013)
Publisher : Institute of Topical Disease Universitas Airlangga

Background: In Indonesia has four serotypes, the DEN-1, DEN-2, DEN-3 and DEN-4. The management of Dengue virus becomes difficult because the patients were infected with different clinical profiles depending on the serotypes and genotypes of infecting dengue virus. Consequently, the diagnosis and treatment becomes difficult. Aim: The purpose of this study was to identify the difference between the results of laboratory tests between non-dengue fever and positive dengue fever. Method: This study was an observational cross-sectional study. Fifteen samples were diagnosed with dengue fever and fifteen samples with negative dengue fever on NS1, IgM / IgG-anti-DHF strip test results. Laboratory tests comprising whole blood, SGOT, SGPT, and TNFa were first examined when the patient came to the hospital. The collected data were analyzed by Chi-Square test SPSS version 13 for Windows. Result: The results of the study in two groups regarding sex, age, days of fever, grade, hemoglobin levels, leukocytes count, platelet count, hematocrit percentage, SGOT levels, and TNFa level were not significantly different with p > 0.050, whereas the SGPT level in non-dengue increased 3 x of normal value of 66.7%, n = 10/15 and in positive dengue fever the SGPT level was within normal limits, found in 60%, n = 9/15, so there was significant difference with p = 0.022 (p < 0.05). Conclusion: in non-dengue SGOT and SGPT levels increased of 1–3 times the normal value. In positive dengue fever SGOT levels increased 1–3 x normal value, but SGPT levels was within normal value, so SGPT levels can be used as a predictive factor for distinguishing the two types of fever.

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