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All Journal EKSAKTA: Journal of Sciences and Data Analysis BioWallacea Journal of Biological Research Majalah Obat Tradisional Jurnal Matematika Sains dan Teknologi Jurnal Biologi Tropis Biosel: Biologi Science and Education Scripta Biologica MAKILA: Jurnal Penelitian Kehutanan Biofaal Journal Molucca Medica Rumphius Pattimura Biological Journal
Efraim Samson, Efraim
Department Of Biology, Universitas Kristen Satya Wacana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Energy Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary Other

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ANALISIS FILOGENETIK GEN gyrA DAN gyrB DARI GENUS MESORHIZOBIUM, RHIZOBIUM DAN ENSIFER Apituley, Edwin Thomas; Samson, Efraim; Killay, Amos
Biofaal Journal Vol 4 No 2 (2023): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v4i2pp118-127

Abstract

Nucleotide sequence of gyrA and gyrB genes encoding subunit A and B of gyrase enzyme from genus of Rhizobium, Ensifer and Mesorhizobium were analyzed to determine evolutionary relationship among them. Maximum Likelihood and Kimura 2 parameter methods were used to construct phylogenetic tree and measure genetic distance. Phylogenetic tree based on nucleotide sequence of gyrA gene was compared to phylogenetic tree based on nucleotide sequence of gyrB gene. Comparison of identity percentage among nucleotide sequence of gyrA gene from genus Rhizobium, Ensifer and Mesorhizobium show similarity, where each of them have narrow range of identity percentage, however nucleotide sequence of gyrB gene from genus Rhizobium and Ensifer show wider identity percentage range than genus Mesorhizobium. Several member of genus Rhizobium and Ensifer have double copy of gyrB gene with identity percentage less than 50 percent between them. Topology of phylogenetic tree based on nucleotide sequence of gyrA gene have similar topology to topology of phylogenetic tree based on nucleotide sequence of gyrB gene, except for additional branch formed by one of additional copy of gyrB sequences. .
POTENSI KULIT BATANG Cinnamomum burmannii Bl. DALAM MENCEGAH INFERTILITAS; KAJIAN TERHADAP BERAT TESTIS DAN JUMLAH SPERMATOZOA MENCIT (Mus musculus) MODEL DIABETES MELLITUS TIPE-1 Moniharapon, Mechiavel; Ukratalo, Abdul M; Pattimura, Nadira; Samson, Efraim; Pangemanan, Victory O
Biofaal Journal Vol 4 No 2 (2023): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v4i2pp108-117

Abstract

In individuals with diabetes mellitus, the increase in Reactive Oxygen Species (ROS) can damage the mitochondrial membrane, leading to the loss of potential mitochondrial membrane function in inducing apoptosis of sperm cells. Cinnamomum burmannii is a type of spice that contains antioxidants, thus capable of neutralizing free radicals and reducing oxidative stress caused by diabetes mellitus. Flavonoids also have an effect on increasing spermatogenesis. The aim of this research is to examine the role of methanol extract from Cinnamomum burmannii bark in increasing testis weight and spermatozoa count in type-1 diabetes mellitus mice (Mus musculus). The study employs a Completely Randomized Design (CRD) with five treatments and three replications. Twenty mice are divided into groups: K- (control), K+ mice injected with STZ without medication), P1 (dose of 200 mg/kg BW), P2 (dose of 400 mg/kg BW), and P3 (dose of 800 mg/kg BW). Before administering the Cinnamomum burmannii bark extract, blood sugar levels are measured in the K+, P1, P2, and P3 groups. If blood sugar levels have increased (>128 mg/dL), the P1, P2, and P3 groups are given the Cinnamomum burmannii bark extract according to the predetermined dosage. The treatment is carried out for 14 days. On the last day, surgery is performed to extract the testes and observe the spermatozoa count. The observation data on testis weight and spermatozoa count will be analyzed using ANOVA with SPSS 24.0. The results indicate that the administration of methanol extract from Cinnamomum burmannii bark significantly affects the increase in testis weight and spermatozoa count in type-1 diabetes mellitus mice. The increase in testis weight and spermatozoa count is proportional to the dosage given
Keanekaragaman dan Dominasi Jenis Pohon Di Kawasan Konservasi Pulau Pombo Kecamatan Salahutu Kabupaten Maluku Tengah Wakano, Deli; Kalsum, Umi; Samson, Efraim
MAKILA Vol 15 No 1 (2021): Makila : Jurnal Penelitian Kehutanan
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (968.539 KB) | DOI: 10.30598/makila.v15i1.3321

Abstract

Pombo Island has a high diversity of trees, these trees have a very important role in the forest community and function as life support. However, until now there have not been many supporting data to identify tree species on Pombo Island. The purpose of this study was to determine tree species, tree diversity and dominance in Pombo Island, Salahutu District, Central Maluku Regency. This research used transect and quadrant methods. The results of this study found 15 tree species, 29 individuals with a tree species diversity value of 2.5775, which means that the tree species in the Pombo Island area are in the medium category and the dominance value is 0.0802, which means that there are no dominant species.
EFFECTIVENESS OF FINGER LEAF INFUSION (Psophocarpus tetragonolobus (L.) DC) ON THE MALARIA MODEL OF RATS (Rattus norvegicus) Kaihena, Martha; Samson, Efraim
RUMPHIUS Vol 1 No 1 (2019): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv1i1p025-032

Abstract

Winged bean is a type of plant that is used as traditional medicine. This study aims to determine the effectiveness of winged bean leaf infusion (Psophocarpus tetragonolobus(L.) DC) against white rat (Rattus norvegicus) model of malaria. The method used in this research is completely randomized design with 4 treatment groups and 3 replications consisting of a control group without being given an infusion (P1), a group that was given an infusion of concentrations: 31.25 mg/mL (P2), 62.5 mg/ mL(P3), and 125 mg/mL (P4). This study used 20 male rats, of which 5 rats including as donor mice. Donor mice were infected with Plasmodium berghe and left until the parasitemia percentage reached> 20%. Then 4 groups of model mice were infected with Plasmodium berghei. Observations were made for 7 days, starting from day 0 (before treatment), 4 days during treatment and 2 days after treatment. The percentage of parasitemia was calculated starting from the day before administration of winged bean leaf infusion (IDK) until the 7th day. The results of this study indicate that IDK can inhibit parasite growth by reducing the level of parasitemia along with increasing concentrations, namely (P2) of 73.78%; (P3) of 89.33%; and (P4) of 93.69%. It can be concluded has potential in inhibiting the growth of Plasmodium berghei in the rat model of malaria with an effective concentration of 31.25 mg/mL.
Co-Authors Amos Killay Apituley, Edwin Thomas CECILIA ANNA SEUMAHU Chomsa Dintasari Umi Baszary Daniati Kasale Deli Wakano Deli Wakano Deli Wakano Ferdy S. Rondonuwu Ferdy S. Rondonuwu Haryono Semangun Haryono Semangun L D Tetelepta La Eddy Martha Kaihena Melati Sopacua Moniharapon, Mechiavel Pangemanan, Victory O Pattimura, Nadira Ukratalo, Abdul M Umi Kalsum Umi Kalsum Vergenia Sigmarlatu Wahyudi Abdul Hamid Ridwan Wakano, Deli Wempi Elwuar