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All Journal Dental Journal (Majalah Kedokteran Gigi) Journal of Dentomaxillofacial Science Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya Conservative Dentistry Journal Indonesian Journal of Dental Medicine Indian Journal of Forensic Medicine & Toxicology

Latief Mooduto, Latief

Department Of Conservative Dentistry, Faculty Of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia

Author-ID : 517971

Dentistry Health Professions Medicine & Pharmacology Public Health

Published : 18 Documents Claim Missing Document

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Articles

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DAYA ANTIBAKTERI EKSTRAK DAUN KEDONDONG BANGKOK (Spondias dulcis Forst.) TERHADAP BAKTERI Enterococcus faecalis Antibacterial Potency of Kedondong Bangkok Leaves Extract (Spondias dulcis Forst.) against Enterococcus faecalis Bacteria Singgih Harseno; Latief Mooduto; Eric Priyo Prasetyo
Conservative Dentistry Journal Vol. 6 No. 2 (2016): July - December
Publisher : Universitas Airlangga

Background. The prevalence of endodontic infection after root canal treatment caused by the Enterococcus faecalis bacteria ranged between 24-77%. It is caused by resilience and virulence from Enterococcus faecalis. An alternative solution have to be done toward irrigation of root canal wall which is effective to kill bacteria. Kedondong Bangkok (Spondias dulcis Forst.) is one of the plants or natural substance potentially as an antibacteria. The antibacterial potencies of Kedondong Bangkok leaves extract (Spondias dulcis Forst.) against Enterococcus faecalis bacteria could be identified by determining Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC). Purpose. This study is aimed to prove antibacterial potencies by identifying Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) of Kedondong Bangkok leaves extract (Spondias dulcis Forst.) against Enterococcus faecalis bacteria. Method. This study is an experimental laboratories through research design of The Post Test Only Control Group Design. Value of MIC and MBC were known by counting the growth of Enterococcus faecalis bacteria by treating the leaves extract of Kedondong Bangkok with concentration respectively 25%, 22,5%, 20%,17,5%, 15%, and 12,5% on nutrient agar media in CFU/ml. Result. In the concentration 12,5% there are 8.8% bacterial growth and in the concentration 15% there are no bacterial growth. Conclusion. Kedondong Bangkok leaf extract (Spondias dulcis Forst.) has an antibacterial potency against Enterococcus faecalis bacteria. The MIC shows in concentration of 12,5% and the MBC shows in concentration of 15%.

Daya bunuh dan daya hambat antimikrobial chlorhexidine 2% dan povidone iodine 1% sebagai medikamen saluran akar terhadap Enterococcus faecalis The ability of chlorhexidine 2% and povidone iodine 1% as root canal medicaments to kill and inhibit Enterococcus faecalis Nathania Astria; Ari Subiyanto; Latief Mooduto
Conservative Dentistry Journal Vol. 7 No. 1 (2017): January - June
Publisher : Universitas Airlangga

Background. Enterococcus faecalis is one of the pathogenic organisms associated with root canal treatment failure and apical periodontitis. Root canal medicament is needed to prevent re-infection in the root canal and increase the success of treatment. Chlorhexidine and povidone iodine is a broad spectrum root canal medicaments that can kill gram-positive bacteria. Purpose. The purpose of this study was to discover the ability to kill and inhibit of antimicrobial chlorhexidine 2% and 1% povidone iodine asvroot canal medicaments against bacteria Enterococcus faecalis. Methode. This research was done by measuring the inhibition zone and count the number of colonies. Determination of the inhibition of root canal medicaments against Enterococcus faecalis by diffusion method. 10 microliter root canal medicaments dropped on paperdisk and placed on nutrient agar media with enterococcus faecalis, then inhibition zone was calculated. Determination ability to kill enterococcus faecalis is done by inserting 1 ml medicaments root canal into 5 ml BHIB media, then 0.05 ml inoculum of Enterococcus faecalis inserted into each tube, except the negative control. 0.1 ml of each tube implanted in the media nutrient agar. Media incubated for 24 hours, then Enterococcus faecalis bacterial colonies that grow in media calculated using the CFU. Results. There no colony growth of enterococcus faecalis in both root canal medicaments. There are significant differences in inhibition zone of 2% chlorhexidine and 1% povidone iodine (p<0.05). Conclusion. Both of root canal medicaments can kill enterococcus faecalis, but chlorhexidine 2% was more capable inhibit Enterococcus faecalis.

Effectiveness of flavonoid from mangosteen pericarp (Garcinia mangostana L.) as Enterococcus faecalis antibiofilm Dalhar Hakiki; Latief Mooduto; Ketut Suardita; Dian Agustin Wahjuningrum
Conservative Dentistry Journal Vol. 7 No. 1 (2017): January - June
Publisher : Universitas Airlangga

Background:Enterococcus faecalis (E. faecalis) is a microorganism that is commonly found in endodontic failure treatment, this due to several characteristics of E.faecalis which has the capabillity to living in environments with high salt levels, high temperature, and pH broad spectrum. Bacteria in biofilms form is one of the adaptive process that allows bacteria to survive in an environment with low nutrients in the root canals. Bacteria in biofilms form have different characteristics from planktonic form, resistance to phagocytic cells and drugs, which can effect to persistent infection. Mangosteen (Garcinia mangostana) has many benefits, especially on the pericarp of the fruit contains alkaloids, tannins, phenolics, flavonoids, and triterpenoids. Flavonoids are the largest group of phenolic compounds that have a nature effectively inhibit the growth of viruses, bacteria, and fungi. Purpose:Purpose of this study wasto find out the role of the antibiofilm of the flavonoid in garcinia mangostana pericarp against E. faecalis bacterial biofilm. Methods:Laboratory experimental in-vitro with post test only group design. The method used is microtitter plate biofilm assay and continued with the readings use Elisa reader at a wavelength of 595 nm. Results:Flavonoids mangosteen pericarp effective as antibiofilm E.faecalis bacteria at a concentration of 12.5%. Conclusion:The study showed that flavonoids from mangosteen pericarp has antibiofilm activity against E. faecalis bacterial biofilm.

Effect of Epigallocatechin-3-Gallate (EGCG) on The Number of Lymphocyte Cells in Inflammation of Pulp with Mechanical Injury Kun Ismiyatin; Latief Mooduto; Putri Dea Amani Faadhilah
Conservative Dentistry Journal Vol. 10 No. 1 (2020): January-June
Publisher : Universitas Airlangga

Background: Pulpitis is an inflammatory pulp that can caused by pulp perforation by mechanical injury. Emergency treatment of pulpitis is using Eugenol. High concentrations of Eugenol have a cytotoxic effect that causes local necrosis and inhibits the healing process. Because of negative effects from eugenol, then it’s necessary to consider a new ingredient with minimal side effects, and it’s epigallocatechin-3-gallate (EGCG) in green tea. As a polyphenol, it has good antioxidant effect and plays a role in shortening the duration of inflammation by radical scavenging against Nitric Oxide so that NO levels rapidly decrease which causes migration of neutrophil cells to the area of injury will decrease and the inflammatory process faster so that the healing process become faster. Lymphocyte is plays a role in tissue repair. Purpose: to explain the effect of EGCG hydrogel on the number of lymphocyte cells in pulp inflammation with mechanical injury. Methods: The study used 24 Wistar rats divided into four groups, namely control (C), and 3 groups of treatment (T1, T2, T3). Each group consisted of 6 rats prepared, then EGCG hydrogel 60 ppm was named T1, EGCG hydrogel 90 ppm was named T2, EGCG hydrogel 120 ppm was named P3. On the 3rd day, Wistar rats were decapitated for HPA preparations. Results: This study showed a significant difference in each group (p < 0.05) using One-Way Anova analysis. Conclusion: EGCG hydrogel 90 ppm is effective in increasing the number of lymphocyte cells in inflammation of the pulp with mechanical injury.

Hemisection of a severely decayed mandibular molar: Case report Cendranata Wibawa Ongkowijoyo; Latief Mooduto; Deavita Dinari; Riski Setyo Avianti
Conservative Dentistry Journal Vol. 10 No. 1 (2020): January-June
Publisher : Universitas Airlangga

Introduction: Dental implants gained popularity as the treatment to replace grossly decayed teeth. However, with the increasing evidence and the difficulty to manage complications associated with dental implants, clinicians are pushed to opt for a more conservative approach. Case report: A male patient with a chief complaint of frequent food impaction in a severely decayed mandibular molar wished to retain his tooth. Upon thorough examination, the carious lesion extended to furcal area that rendered the distal root unsalvageable. However the mesial root can be retained; thus, hemisection was proposed. Management: Root canal treatment was carried out in the mesial canals. Then, the tooth was split mesio-distally.The distal root was extracted, and the mesial root was retained. Subsequently, the tooth was restored with PFM crown.Conclusion: Hemisection with subsequent prosthetic rehabilitation can be a viable alternative to retain severely decayed mandibular molar.

Antibacterial potency of mangosteen pericarp extracts (Garcinia mangostana L.) against Fusobacterium nucleatum Nia Pramais Octaviani; Latief Mooduto; Achmad Sudirman
Conservative Dentistry Journal Vol. 10 No. 2 (2020): July-December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v10i2.2020.44-47

Background: Fusobacterium nucleatum is a common bacterial in root canal with pulp necrosis and periradicular lesion. A way to eliminate these bacteria from root canal is by root canal irrigation. Root canal irrigation materials that are widely used nowadays has many shortcomings. The pericarp of mangosteen (Garcinia mangostana L) has antibacterial potency. Therefore mangosteen pericarp can be an alternative material which could inhibit and bactericidal function to Fusobacterium nucleatum. Purpose: The aim of this study was to determine the antibacterial potency of mangosteen pericarp extract (Garcinia Mangostana L.) against Fusobacterium nucleatum. Methods: This study was laboratory experimental with pos-test only control group design. A microdilution method was used to determine minimum inhibitory concentration and minimum bactericidal concentration by colony counting bacteriae in Tryptone Soya Agar (TSA) media with drop plate technique. Growth of bacterial colonies in TSA is calculated manually in colony forming unit (CFU/ml). Results: Bacterial colonies growth at concentration 0.78% was 90% less than positive control group and there were no bacterial colonies growth at concentration 0.975%. Conclusion: The Minimum Inhibitory Concentration (MIC) of mangosteen pericarp against Fusobacterium nucleatum was at 0,78% concentration and the Minimum Bactericidal Concentration (MBC) was at 0.975% concentration.

Effectiveness of Sealer Combination Zinc Oxide (ZnO) with Red Pine (Pinus densiflora) on Viscosity and Solubility (Laboratory Experimental) Sukaton Sukaton; Latief Mooduto; Stheril Andani
Conservative Dentistry Journal Vol. 11 No. 1 (2021): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v11i1.2021.6-10

Background: Failure of endodontic treatment is caused by 60% of poor obturation. Sealers have an important role in the obturation process, that is filling the root canals and the space between the dentin and the core material. Sealers must have low viscosity so that they can flow easily and fill the entire root canal space so can form a good seal and have physical properties that are not easily soluble to oral fluids to prevent sealer degradation which can cause gaps during filling and become a medium for bacterial proliferation. Red pine extract is a natural ingredient that contain flavonoid and phenolic acid that are effective in reducing the viscosity and solubility of the sealer combination ZnO with red pine. Purpose: To determine the effectiveness of sealer combination ZnO with red pine in reducing viscosity and solubility. Methods: Red pine (Pinus densilora) extract with a concentration of 100% was diluted to a concentration of 0.78% using the dilution formula M1.VI = M2.V2. Viscosity test was done by mixing ZnO with red pine extract 0.78% 1: 1 and calcium hydroxide (Ca(OH)2) with 1: 1 sterile aquadest as a comparison, then each sample was measured for viscosity using a brookfield viscometer. The solubility test was carried out by placing the paste on a disc with a diameter of 15 mm x 3 mm and then being set and immersed in distilled water for 24 hours then the percentage of solubility was calculated. Results: The viscosity value of combination ZnO with red pine is 19.89 Pa.s and the solubility is 0.0075%, while Ca(OH)2 with sterile aquadest had a viscosity of 23.32 Pa.s and a solubility is 0.029%. Conclusion: The combination of ZnO with red pine is effective in reducing the viscosity and solubility of the combination sealer. The viscosity and solubility of the combined sealer is lower than Ca(OH)2 with sterile aquadest.

Comparison of ratios combination calcium hydroxide Ca(OH)2 with red pine (Pinus densiflora) of viscosity Latief Mooduto; Ari Subiyanto; Shafa Prasita
Conservative Dentistry Journal Vol. 11 No. 2 (2021): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v11i2.2021.50-55

Background: Calcium hydroxide is a gold standard for root canal sealing material. However, there are many defects in calcium hydroxide that have prompted many researchers to look for alternative materials, including natural origin ingredients. Red pine (Pinus densiflora) is often used in dentistry for its antioxidant and antibacterial properties. The mixture of calcium hydroxide and red pine has never been achieved before. One of the criteria for material sealing is that the channel should be fully sealed with high flow ability and low viscosity. Therefore, this research is performed to analyze the viscosity value of the mixture of calcium hydroxide and red pine (Pinus densiflora). Purpose: Knowing the difference in viscosity of the combination of calcium hydroxide and red pine with a ratio of 1:1, 1:1.5, and 1:2. Methods: This study used four treatment groups, with each group consisting of 8 replications. Group 1 was a combination of calcium hydroxide and red pine with a 1: 1, group 2 the ratio was 1: 1.5, group 3 had the ratio 1: 2, and the positive control group used calcium hydroxide and a sterile aquadest. Calcium hydroxide in powder form and an extract of red pine in liquid form are mixed according to the ratio. The viscosity value is measured using a viscosity tester, namely the Brookfield Viscometer. Results: The control group had a lower viscosity than group 3, group 3, and group 2 than group 1. Conclusion: Combination of calcium hydroxide and red pine with a ratio of 1:2, the lowest viscosity was obtained compared to the ratio of 1: 1 and 1: 1.5

White pomegranate (Punica granatum) peels extract bactericidal potency on Enterococcus faecalis Eric Priyo Prasetyo; Widya Saraswati; Dian Agustin Wahjuningrum; Latief Mooduto; Rizka Firdaus Rosidin; Evelyn Tjendronegoro
Conservative Dentistry Journal Vol. 11 No. 2 (2021): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v11i2.2021.84-88

Background: Enterococcus faecalis (E. faecalis) is the most resistant bacteria in the root canals and one of the causes of recurrent endodontic treatment. Enterococcus faecalis was not the only bacteria found in failed endodontic treatment but these bacteria can thrive in unfavorable environment. White pomegranate (Punica granatum) is known for its flavonoids and tannins that function as antimicrobial agent. White pomegranate extract is potential for use as disinfection or irrigation material. Purpose: This aim of this study was to determine the bactericidal potency of white pomegranate (Punica granatum) extract on E. faecalis growth. Methods: This study was an in vitro experimental observation. E. faecalis was obtained from stock culture taken from the root canal of recurrent endodontic treatment. E. faecalis from the serial dilution were cultured in blood agar media. Antibacterial potency was determined by colony calculation of E. faecalis growth in blood agar in colony forming unit (CFU) and conducted in 6 replications for each concentration group. Statistical analysis was done using one-way analysis of variance at 5% significance level. Results: White pomegranate peels extract concentrations of 3.125%, 6.25%, 12.5% and 25% provide significant decrease in the number of E. faecalis colony compared to the control group (p<0.05). No bacterial growth was found on 25%, 50% and 100% concentration. Conclusion: The potent minimal bactericidal concentration of white pomegranate peels extract on E. faecalis was 25%.

Co-Authors Achmad Sudirman Ahmad Yudianto Ari Subiyanto Ari Subiyanto, Ari Arif Rahman Nurdianto Cendranata Wibawa Ongkowijoyo Dalhar Hakiki Deavita Dinari Dian Agustin Wahjuningrum Elvira Theola Judith Erawati Wulandari Eric Priyo Prasetyo Evelyn Tjendronegoro Fajar Agus Muttaqin Fery Setiawan Fery Setiawan Harry Huiz Peeters Ika Tangdan Jenny Sunariani Jenny Sunariani, Jenny Ketut Suardita, Ketut Kun Ismiyatin Kuntaman Kuntaman Nathania Astria Nia Pramais Octaviani Nurdianto, Arif Rahman Prasita, Shafa Putri Dea Amani Faadhilah Reinold Christian Lina Revina Ester Iriani Marpaung Riski Setyo Avianti Rizka Firdaus Rosidin Shafa Prasita Singgih Harseno Stheril Andani Sukaton, Sukaton Tamara Yuanita Theresia Indah Budhy Sulisetyawati Widya Saraswati Yulianti Kartini Sunur Yuliati

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