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Konstruksi Vektor Biner Mengandung Gen Hibrid cryIB-cryIAa untuk Transformasi Agrobacterium Tanaman Padi (Oryza sativa L) Rahmawati, Syamsidah; Slamet-Loedin, Inez H.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2004): June 2004
Publisher : Universitas Atma Jaya Yogyakarta

This study aimed to obtain binary vectors carryingÂ cryIB-cryIAa hybrid gene suitable for Agrobacterium-mediated transformation of two Indonesian rice cultivars, Rajalele (Javanica) and Cisadane (Indica).Â Introduction of hybrid Bt toxin, encoded by cryIB-cryIAa hybrid gene with different binding receptor in the insect gut driven by maize ubiquitin promoter, was intended to reduce the possibility of the appearance of stem borer resistant to Bt toxin. A HindIII fragment containing the ubi cryIB-cryIAa hybrid gene was excised from pBKS ubi cryIB-cryIAa and cloned into the HindIII site of binary vector pCAMBIA 1301, 1303, or 1304.Â Restriction analysis of recombinant plasmids confirmed the insertion of the gene and demonstrated that the gene inserted in two orientations. The recombinant plasmids were transformed into electrocompetent Agrobacterium tumefaciens EHA105 by electroporation.Â Embryogenic calli of Rajalele and Cisadane were co-cultivated with A. tumefaciens EHA 105 harbouring binary vector pCAMBIA 1301, 1303, or 1304 containing ubi cryIB-cryIAa. GUS histochemical assay revealed that high transformation efficiency (75-100%) on both cultivars were obtained from embryogenic calli transformed with pCAMBIA 1303 or 1304.

Overexpression of OsHox-6 Gene Enhanced Tiller Number in Rice But Induced Yield Penalty Rahmawati, Syamsidah; Chairunnisa, Chairunnisa; Erdayani, Eva; Nugroho, Satya; Estiati, Amy
ANNALES BOGORIENSES Vol 23, No 1 (2019): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

OsHox-6, belongs to the transcription factor homeodomain leucine zipper (HD-Zip) protein sub-family I, has unknown function. This study was aimed to characterize the phenotypes of two homozygous transgenic rice lines (S29-62-2 and S.40.4-158-1) containing an extra copy of OsHox-6 gene under the control of a rice constitutive promoter, OsLEA3, and to evaluate their tolerance to water stress. A real-time quantitative PCR (qRT-PCR) showed that the transcript expression of OsHox-6 gene in the transgenic lines increased 5-10 folds under a normal irrigation and 10-20 folds after exposure to water stress conditions as compared to its wild type control. Transgenic plants overexpressing OsHox-6 exhibited phenotypic alteration at the normal irrigation by inducing tiller formation, suggesting a decrease in the apical dominance. Transgenic plants also showed significant enhancement in the total grain number, however, the number of empty grains also increased significantly (~16-22%). After imposed to the water stress, the number of empty grains in the transgenic lines was even higher (up to 83% in average). Furthermore, observations on the water loss rates, relative water contents and drought resistance indices (DRI) suggested that the overexpression of OsHox-6 did not significantly increase tolerance to water stress. Further research is required to reveal the detailed mechanisms of OsHox-6 in response to water and other abiotic stresses.

Consumer Preference Study on Myrtaceae Fruit Collection of Bogor Botanic Gardens Martiansyah, Irfan; Hariri, Muhammad Rifqi; Mulyani, Melza; Husaini, Iin Pertiwi A; Hidayat, Arief; Rahmawati, Syamsidah
Industria: Jurnal Teknologi dan Manajemen Agroindustri Vol 10, No 1 (2021)
Publisher : Department of Agro-industrial Technology, University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.industria.2021.010.01.5

AbstractMyrtaceae fruit in Bogor Botanic Gardens (BBG) can be potentially developed as a unique and new fruit consumption style. The study aimed to determine the consumer preferences on Myrtaceae fruit. The method used was organoleptic testing, including a description, hedonic and rank tests. The parameters measured in this study are taste, color, flavor, texture, sound, and size of the fruit. Data analysis performed by the Kruskal-Wallis test. The results showed that most of the respondents preferred the fruit with a medium-size, tastes sour but not bitter, has attractive colors, has no pungent flavor, has soft and smooth texture, and is not crispy when it is bitten and chewed. The hedonic test showed that sample #4 and #7 have the highest average value of 4.7. The lowest average obtained in sample #3, where respondents did not like the sample. There was no significant difference of the average. The ranking of hedonic test result indicates the level of respondents' preferences in sequence are Eugenia sp. from Southeast Sulawesi, Myrtus pendula, Syzygium bankense, Syzygium aqueum, Eugenia sp. from Papua, Syzygium sp. from Seram Island, and Syzygium polycephaloides.Keywords: Bogor Botanic Gardens, consumer preference, Myrtaceae fruits, organoleptic test AbstrakBuah jambu-jambuan koleksi Kebun Raya Bogor berpotensi dijadikan buah konsumsi baru. Tujuan penelitian adalah untuk mengetahui dan menilai preferensi serta tingkat kesukaan masyarakat terhadap jenis buah jambu-jambuan koleksi Kebun Raya Bogor. Metode yang digunakan adalah uji organoleptik melalui metode uji deskriptif, uji kesukaan, dan uji rangking. Parameter uji meliputi rasa, warna, aroma, tekstur, suara, dan ukuran. Analisis data dilakukan secara deskriptif dan non-parametrik Kruskal-Wallis. Hasil penelitian memperlihatkan sebagian besar responden menyukai jenis buah berukuran sedang, rasa dominan masam tetapi tidak pahit, warna mencolok, aroma tidak menyengat, tekstur lunak dan halus, serta tidak renyah saat digigit. Hasil uji kesukaan menunjukan nilai rata-rata tertinggi sebesar 4,7 terdapat pada sampel #4 dan #7. Rata-rata terendah ditunjukkan pada sampel #3 sebesar 3,0, yaitu responden tidak menyukai sampel tersebut. Perbedaan rata-rata tersebut tidak signifikan. Perangkingan hasil uji kesukaan menunjukkan tingkat kesukaan responden secara berturut-turut adalah Eugenia sp. asal Sulawesi Tenggara, Myrtus pendula, Syzygium bankense, Syzygium aqueum, Eugenia sp. asal Papua, Syzygium sp, asal Pulau Seram, dan Syzygium polycephaloides.Kata kunci: buah jambu-jambuan, Kebun Raya Bogor, preferensi konsumen, uji organoleptik

A Comparative Study of the Organellar Genome of Gyrinops versteegii and Aquilaria malaccensis Hartati, Hartati; Cartealy, Imam Civi; Supatmi, Supatmi; Rahmawati, Syamsidah; Hartati, N Sri; Siregar, Ulfah Juniarti; Siregar, Iskandar Zulkarnaen
Jurnal Manajemen Hutan Tropika Vol. 30 No. 3 (2024)
Publisher : Institut Pertanian Bogor (IPB University)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.7226/jtfm.30.3.326

Gyrinops versteegii and Aquilaria malaccensis are two important species of the Aquilarieae tribe. The main problem of this tribe is the challenge of species identification that is strongly dependent on the presence of flowers and fruit, which are not always available. The availability of whole genome information is expected to address the problems of species identification. This research aims to construct and compare the chloroplast and mitochondrial genomes of G. versteegii and A. malaccensis from short-read data using the NOVOplasty and GetOrganelle assembler. The chloroplast genome assembly revealed a full-length quadripartite circular structure with sizes of 174.814 bp (G. versteegii) and 174.821–174.822 bp (A. malaccensis), with highly conserved gene and organization. Meanwhile, the mitochondrial genome is multipartite with a size of 400.012 bp (G. versteegii) and 400.000 bp (A. malaccensis), with highly variable genes and organization due to the presence of gene cluster repeats. The LSC/IR/SCC region borders and phylogenetic analysis in chloroplasts indicate variations between the genomes of these two species. The investigation of nucleotide diversity in the chloroplast genome revealed that the trnL-rpl32 region had the highest nucleotide diversity (Pi = 0.03). This information will be useful in the future for a variety of downstream analyses.

Overexpression of OsHox-6 Gene Enhanced Tiller Number in Rice But Induced Yield Penalty Rahmawati, Syamsidah; Chairunnisa, Chairunnisa; Erdayani, Eva; Nugroho, Satya; Estiati, Amy
Annales Bogorienses Vol. 23 No. 1 (2019): Annales Bogorienses
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar

OsHox-6, belongs to the transcription factor homeodomain leucine zipper (HD-Zip) protein sub-family I, has unknown function. This study was aimed to characterize the phenotypes of two homozygous transgenic rice lines (S29-62-2 and S.40.4-158-1) containing an extra copy of OsHox-6 gene under the control of a rice constitutive promoter, OsLEA3, and to evaluate their tolerance to water stress. A real-time quantitative PCR (qRT-PCR) showed that the transcript expression of OsHox-6 gene in the transgenic lines increased 5-10 folds under a normal irrigation and 10-20 folds after exposure to water stress conditions as compared to its wild type control. Transgenic plants overexpressing OsHox-6 exhibited phenotypic alteration at the normal irrigation by inducing tiller formation, suggesting a decrease in the apical dominance. Transgenic plants also showed significant enhancement in the total grain number, however, the number of empty grains also increased significantly (~16-22%). After imposed to the water stress, the number of empty grains in the transgenic lines was even higher (up to 83% in average). Furthermore, observations on the water loss rates, relative water contents and drought resistance indices (DRI) suggested that the overexpression of OsHox-6 did not significantly increase tolerance to water stress. Further research is required to reveal the detailed mechanisms of OsHox-6 in response to water and other abiotic stresses.

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