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All Journal Biology, Medicine, & Natural Product Chemistry Jurnal Media Gizi Indonesia (MGI) Scientiae Educatia: Jurnal Pendidikan Sains Jurnal Biodjati Biosaintifika: Journal of Biology & Biology Education Journal of Stem Cell Research and Tissue Engineering Jurnal Pengabdian Sosial Life Science and Biotechnology Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Biodiversitas dan Bioteknologi Scientiae Educatia: Jurnal Pendidikan Sains Nusantara Bioscience
Haryanto Haryanto
Department of Biotechnology, Faculty of Science and Technology, Muhammadiyah University of Bandung
Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Viability of Lactobacillus bulgaricus and Streptococcus thermophilus in Encapsulated Probiotic Candy With Freeze-Dry Method Agus Sutandi; Haryanto Haryanto; Eni Kusumawati
Scientiae Educatia: Jurnal Pendidikan Sains Vol. 11 No. 1 (2022): June 2022
Publisher : Tadris Biologi Fakultas Ilmu Tarbiyah dan Keguruan IAIN SYEKH NURJATI CIREBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24235/sc.educatia.v11i1.10159

Abstract

The level of viable cell count in probiotic candy, based on WHO standard is >106 CFU/ml or >106 CFU/g. Meanwhile, information on the bacterial viability of probiotic candy according to WHO standards is still limited.  The experimental study was conducted to discover the viability of Lactobacillus bulgaricus and Streptococcus thermophilus in encapsulated probiotic candy. Encapsulants consisted of maltodextrin, gum, corn starch, and skim milk. Encapsulation of probiotic bacteria used freeze-dry method. Probiotic candies were stored at room temperature for 14 days in an aerobic condition. The viability of probiotics candy was tested and the results showed significance for each type of encapsulation (p<0.05). The gum and corn starch encapsulations showed bacteria  viability that met WHO standards for functional foods, which ranged from 20,333,333±7,637,626to 31,553,333±2,741,894 CFU/g (>106 CFU/g). The most preferred encapsulant in terms of taste, texture, and aroma was identified as skim milk encapsulation. Further research on the long-term storage of probiotic candy and the viability of probiotic candy bacteria in the gastrointestinal tract (in vivo) is needed.
Weissella paramesenteroides: A Lactic Acid Bacteria Producing Glutathione from Fermented Vegetable Based Keciwis Leaf Luthfia Hastiani Muharram; Haryanto; Wulan Pertiwi; Nelis Hernahadini; Ima Mukaromah
Jurnal Biodjati Vol 10 No 1 (2025): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v10i1.30589

Abstract

Glutathione (GSH), is one of the essential antioxidants to prevent free radicals and support the immune system. The low level of glutathione is associated with some diseases such as cancer, Alzheimer's, Parkinson's, and AIDS. Lactic acid bacteria (LAB) can produce glutathione. Indonesia, as a mega biodiversity country, has various vegetables and LAB sources that remain underexplored. This research aims to explore LAB-producing glutathione from fermented products derived from Indonesia's typical plant, the keciwis leaf. The method of this research includes sample preparation, lactic acid bacteria isolation, secondary metabolite production with cysteine precursor addition, glutathione analysis by Ellman's assay, identification of 16S rRNA gene of lactic acid bacteria, and glutathione qualitative analysis by HPLC.  Fermented keciwis leaf-based products contain lactic acid bacteria with positive gram characteristics, can produce 0.33 – 0.37 mM extracellular glutathione. Precursor cysteine addition significantly increases glutathione (p<0,05). The selective lactic acid bacteria producing glutathione was identified as belonging to the genus Weissella, with a similarity value of 98.50%, closely related to Weissella paramesenteroides. Extracellular glutathione produced by this culture showed qualitative results on HPLC with a retention time of 6.34 minutes, indicating the presence of the sulfihydryl group. This study identified a new Indonesia source of lactic acid bacteria of Indonesian–origin lactic acid bacteria, specifically Weissella paramesenteroides, as a promising source for glutathione production
Effect of light and media composition on growth and stomatal density of Phalaenopsis amboinensis MUHAMMAD ALANWARI NUGRAHA; DWI MURTI PUSPITANINGTYAS; HARYANTO HARYANTO
Nusantara Bioscience Vol. 17 No. 2 (2025)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n170207

Abstract

Abstract. Nugraha MA, Puspitaningtyas DM, Haryanto. 2025. Effect of light and media composition on growth and stomatal density of Phalaenopsis amboinensis. Nusantara Bioscience 17: 243-252. Phalaenopsis amboinensis J.J.Sm. is an endangered orchid species endemic to Indonesia, valued for both its conservation significance and horticultural potential. However, limited natural populations and slow propagation rates hinder sustainable conservation efforts. This study investigated the combined effects of culture media composition and light conditions on the in vitro growth, chlorophyll content, and stomatal density of P. amboinensis plantlets. A factorial experiment was conducted using nine media formulations under light and dark conditions. Significant interactions between media and light treatments were observed across all measured parameters (p &lt; 0.05). The KC EO2 medium, containing 2 g/L KC, 40 g/L potato (Solanum tuberosum L.) extract, 2 g/L peptone, and MS vitamins under light, produced the most vigorous vegetative growth (5.40 ± 2.50 leaves, 1.44 ± 0.31 cm leaf length, 2.00 ± 0.82 roots, and 1.89 ± 0.45 cm root length). In contrast, the KC EO3 medium with 2 g/L KC, 150 g/L mung bean sprout (Vigna radiata (L.) R.Wilczek) extract, and 150 mL/L young coconut water (Cocos nucifera L.) under light yielded the highest chlorophyll a content (0.695 ± 0.113 mg/g fresh weight) and stomatal density (77.57 ± 2.72 stomata/mm²). Control plantlets cultured without organic supplements under dark conditions showed poor growth and negligible chlorophyll accumulation. These findings demonstrate that organic-enriched media, especially when combined with light exposure, significantly enhance physiological and morphological development in vitro. The optimized culture conditions offer a practical and scalable protocol for ex situ conservation and commercial propagation of P. amboinensis, supporting the preservation and sustainable use of this rare orchid species.
Co-Authors Adillah, Nanda Nur Aditya, Nara Aghniya Nur Rizka Fadila Agus Sutandi Agustina, Nabila Nur Akmal, Muhamad Alaudin, Ahmad Muzaki Nurrah-man Alim, Rofi’ul Andani, Salma Nabila Putri DWI MURTI PUSPITANINGTYAS Eni Kusumawati Fadila, Aghniya Nur Rizka Fadilla, Aghniya Nur Rizka Fadillah, Jihan Hilmi Fadly Hidayaturochman Firdaus, Muhammad Gibran Fitri, Fadillya Maulidilla Ganni, Aldhi Ya Hakim, Elsya Bayduri Haryanti, Indri Hernahadini, Nelis Hikmah, Wulan Adinda Ima Mukaromah Kusumawati, Eni Luthfi, Cahya Arsya Luthfia Hastiani Muharram Maksum, Muhammad Farid Maulidilla Fitri, Fadillya Meisa, Indri Dwi Muhamad Yunus MUHAMMAD ALANWARI NUGRAHA Nafsi, Ginan Najah, Adilla Syahsiyatun Nelis Hernahadini Nuraeni, Sri Wahyuni Nuralim, Aji Nurfadillah, Arni Risma Nurhayati, Sari Padila, Qurni Raflianti, Desti Salsabila, Anbar Rania Salsabila, Aulia Putri Subagio Joko Suprapto Sulasikin, Almaydha Naim Sulastri, Alnofika Dewi Susan Susanti Sya’diah, Alvi Latifatus Wahyudin Wahyudin Wibawa, Alifah Mustari Mukti Wijaya, Nur Iman Wulan Pertiwi