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All Journal JURNAL KIMIA SAINS DAN APLIKASI Medical Laboratory Technology Journal Jurnal Farmamedika (Pharmamedica Journal) BioEksakta : Jurnal Ilmiah Biologi Unsoed Journal of Indonesian Medical Laboratory and Science (JoIMedLabS) Pharmaceutical And Biomedical Sciences Journal (PBSJ) Jurnal Pengabdian Masyarakat dan Riset Pendidikan Health Science & Biomedical Journal (HSBJ) Berita Biologi
Sharfina Maulidayanti
Department Of Medical Laboratory Technologist, STIKes Prima Indonesia|STIKes Prima Indonesia|Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Engineering Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Nursing Public Health Veterinary Other

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ISOLASI DAN KARAKTERISASI MOLEKULAR 16S rRNA PROBIOTIK DARI MINUMAN FERMENTASI KULIT BIJI KOPI SEBAGAI ANTI INFEKSI PENYAKIT TROPIS Maulidayanti, Sharfina; Andini, Futri; Situmorang, Ing Mayfa Br; Tanjung, Asbar; Ningrum , Isra Jannatin
Berita Biologi Vol 24 No 3 (2025): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2025.11939

Abstract

Penyakit infeksi tropis yang disebabkan oleh bakteri Escherichia coli dan Salmonella typhi menjadi tantangan besar dalam dunia kesehatan, terutama dengan meningkatnya kasus resistensi antibiotik. Penelitian ini bertujuan untuk mengeksplorasi potensi probiotik dari minuman fermentasi kulit biji kopi (Coffea sp.) sebagai alternatif alami untuk menghambat pertumbuhan bakteri patogen tersebut. Isolasi dan karakterisasi fenotipik dilakukan terhadap bakteri asam laktat (BAL), meliputi pengamatan morfologi koloni, pewarnaan Gram, uji katalase, dan uji motilitas. Selain itu, dilakukan uji ketahanan terhadap pH rendah dan garam empedu, serta uji daya hambat terhadap E. coli dan S. typhi. Hasil menunjukkan bahwa isolat BAL yang diperoleh memiliki ciri khas, yaitu morfologi koloni bulat putih, bersifat Gram positif, non-motil, dan katalase negatif. Isolat juga mampu bertahan pada kondisi ekstrem, yakni pH 3 hingga 7 serta garam empedu 3%. Uji daya hambat menunjukkan kemampuan antibakteri yang baik, dengan zona hambat terhadap E. coli sebesar 27,25 mm dan terhadap S. typhi sebesar 22,5 mm, yang termasuk dalam kategori susceptible hingga intermediate. Analisis genotip melalui sekuensing gen 16S rRNA menunjukkan bahwa isolat memiliki persentase homologi sebesar 98,7% dengan Lacticaseibacillus rhamnosus, mengidentifikasikannya sebagai spesies tersebut. Dengan demikian, Lacticaseibacillus rhamnosus dari fermentasi kulit biji kopi menunjukkan potensi sebagai kandidat probiotik alami, yang dapat digunakan dalam pengendalian infeksi bakteri tropis seperti E. coli enteropatogenik dan S. typhi.
Aktivitas Antijamur Ekstrak Biji Kopi Hijau Robusta (Coffea canephora) Terhadap Candida albicans. Ing Mayfa Br Situmorang; Putri Salsadila; Sharfina Maulidayanti; Asbar Tanjung
Pharmaceutical and Biomedical Sciences Journal (PBSJ) Vol. 6 No. 2 (2024)
Publisher : Pharmacy Department, Faculty of Health Sciences, UIN Syarif Hidayatullah Jakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/pbsj.v6i2.39149

Abstract

Acne is a disease that can be caused by bacteria, one of which is Staphylococcus epidermidis. Acne can be treated with active compounds derived from natural ingredients such as cucumbers and tomatoes. Gel masks peel off. This study aims to determine the average diameter of the inhibition zone, the difference in the average diameter of the inhibition zone, and the antibacterial activity of the peel-off from a mixture of cucumber (Cucumis sativus) and tomato  (Solanum lycopersicum) with a concentration of 5%, 10 %, 15%, 20% against  Staphylococcus epidermidis. This experimental study uses a Completely Randomized Design (CRD) consisting of one factor and 6 levels. The results showed that the average diameter of the inhibition zone of the anti-acne peel-off gel mask preparation of cucumber and tomato extracts against Staphylococcus epidermidis at a concentration of 5%, 10%, 15% and 20% were 16.38 mm, respectively. ; 24.88 mm; 29.88 mm and 34.88 mm. There was a significant difference in the average diameter of the inhibition zone of the peel off mixture of cucumber and tomato extracts with a concentration of 5%, 10%, 15%, and 20%; against Staphylococcus epidermidis. gel mask preparation peels off from a mixture of cucumber and tomato extracts against Staphylococcus epidermidis at a concentration of 5%, 10% concentration, 15% concentration and 20% concentration, namely strong, very strong, very strong and very strong.   Keywords: staphylococcus epidermidis, gel mask peel-off, cucumber, tomato
Co-Authors Andini, Futri Asbar Tanjung Ayumi, Irmalita Br Situmorang, Ing Mayfa Deva Azvara Devi Naibaho Dicky Annas Fathia, Mona Feri Setiadi Frida Octavia Purnomo Ing Mayfa Br Situmorang Jumriah Nur, Jumriah Khoirul Anwar Ladyka Viola Aulia Armawan Mahendra, Robert Ariyan Nadila, Syifa Intan Nafis, Nidhal Mijwan Ningrum , Isra Jannatin Noni Farah Nurhidayah, Ika Nuzul Gyanata Adiwisastra Putri Salsadila Ricky Ardiansyah Situmorang, Ing Mayfa Br Tunas Alam Winanda, Witri