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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Ilmu Dasar BERKALA SAINSTEK Jurnal Biologi dan Pembelajarannya (JBP) Biology, Medicine, & Natural Product Chemistry Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Jurnal Sains Alami (Known Nature) Berkala Penelitian Hayati Biopedagogia Jurnal Sains Alami (Known Nature) Exhibition and Seminar on Science and Creative Technology – Al Azhar Proceeding
Gatra Ervi Jayanti
Biology Department, Faculty Of Mathematics And Natural Sciences, Universitas Islam Malang, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Education Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Other

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Balur and Improving Quality of Life Jayanti, Gatra Ervi; Subagjo, Saraswati
Journal of Tropical Life Science Vol 6, No 1 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.01.07

Abstract

Balur treatment aimed to leach free radicals overwhelmingly produced in unhealthy body with rubbed over the human body. In Balur used some amino acid and herbal such as Bawang Sabrang (Eleutherine palmifolia (L.) Merr), Kopi Balur (Mixed Coffea arabica L. with Acetosal), Coconut Water (Cocos nucifera L.), Secang (Caesalpinia sappan L.) and “Divine” smoke from cloves (Syzygium aromaticum (L.) Merr. & L. M. Perry) and Tobacco (Nicotiana tabacum L). This is an observation research of Balur application in volunteers during four years at Rumah Sehat, Malang, Indonesia. The result suggested that Balur can improve quality of life  in male volunteers i.e. 61,54% for Cancer (39 volunteers); 80% for Tumor (20 volunteers); 86,36% for Diabetes mellitus (22 volunteers); 93,33% for Heart (15 volunteers); 92,86% for Autism (28 volunteers); 72,73% for Hepatitis (11 volunteers); 100% neuron disease for (2 volunteers); 93,62% for various of disease (94 volunteers). Moreover imrovement quality of life in female volunteers i.e. 72,31% for Cancer (65 volunteers); 88,57% for Tumor (35 volunteers); 75% for Diabetes mellitus (16 volunteers); 100% for Hemorrhoid (3 volunteers); 100% for stroke (3 volunteers); 40% for Cardiomegaly (5 volunteers); 66,67% for Autism (3 volunteers); 88% for various of disease (50 volunteers) and 100% for healthy (3 volunteers). Keywords: Balur, herb in Balur process, improving quality of life
Scavenging activity nano complex compounds of kelor (Moringa oleifera Lamk.) leaves and seeds Rafida Azizah; Tintrim Rahayu; Ari Hayati; Gatra Ervi Jayanti
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 26 No 1 (2020): December 2020
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (595.361 KB) | DOI: 10.23869/bphjbr.26.1.20205

Abstract

Moringa oleifera Lamk. is a good source of natural antioxidants because it contains various types of antioxidant compounds such as ascorbic acid, flavonoids, phenolics, and carotenoids. Those antioxidant components forming complex structure have transitional metal as central compound, which have free radical scavenging activity. This study aims to determine the active compounds that act as scavenger in leaves and seeds of M. oleifera. The possible compound found in leaves-seeds is elaborated by in silico analysis, using Dr. Duke's Phytochemical and Ethnobotanical Databases, by mean Pass online, and HitPick software. The results of in silico analysis 3 compounds identified in the leaves that had a high antioxidant role, namely beta-carotene, kaempferol, quercetin, and 2 compounds in seeds that had a high antioxidant role, namely alpha-tocopherol, beta-carotene. The results of this study indicate that the antioxidant activity of the 3 treatments had differences effectiveness of antioxidants. All of these antioxidants has ability to bind transitional metal to form free radical scavenger.
Karakterisasi Bunga Tetua Anggrek Dendrobium dalam Menghasilkan Variasi Fenotipe Baru Melalui Teknik Hibridisasi Muhammad Ni'amul Albab; Tintrim Rahayu; Gatra Ervi Jayanti
Buletin Anatomi dan Fisiologi Volume 6, Nomor 2, Tahun 2021
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.6.2.2021.203-211

Abstract

Peningkatan mutu pada tanaman anggrek juga dapat ditingkatkan pada teknik persilangan dan perbanyakan biji hasil persilangan. Kegiatan persilangan banyak menggunakan varietas-varietas dengan tetua yang sama sehingga menyebabkan variasi genetik pada hibrida yang terbentuk menjadi terbatas. Tujuan penelitian ini adalah untuk mendeskripsikan karakteristik bunga tetua anggrek Dendrobium yang digunakan sebagai indukan dan menganalisis bunga pada generasi hasil persilangan. Penelitian ini menggunakan metode kualitatif dengan cara mendeskripsikan karakteristik bunga tetua anggrek dengan variasi berbeda serta bunga pada anggrek hibrida hasil persilangan. Analisis dilakukan melalui pendekatan morfologi secara langsung. Hasil yang diperoleh adalah anggrek hibrida hasil persilangan yang telah berbunga memiliki morfologi bunga yang mirip seperti kedua tetua persilangannya seperti ukuran bunga, warna bunga, bentuk dan warna sepal maupun petal, dan warna dari labellum tergantung dari karakteristik bunga yang terdapat pada kedua tetua persilangannya. Kesimpulan yang didapat berupa pemilihan tetua persilangan ditentukan sesuai karakter bunga yang dikehendaki sehingga menghasilkan generasi yang memiliki variasi bunga baru dan bunga dari anggrek hibrida yang dihasilkan dari pemuliaan anggrek memiliki campuran karakteristik bunga dari kedua tetua anggrek yang digunakan sebagai indukan persilangan, tetapi tidak semua karakter bunga dari kedua tetua muncul, ada beberapa karakter bunga yang muncul dari salah satu tetua. Improving the quality of orchid plants can also be improved on the technique of crossing and seed propagation from crosses. Crossing activities use a lot of varieties with the same parents, causing genetic variation in the hybrids formed to be limited. The purpose of this study was to describe the characteristics of the flower of the Dendrobium orchid parent used as a parent and to analyze the flower in the generation of crosses. This study uses a qualitative method by describing the characteristics of the flower of elder orchids with different variations and the flowers on hybrid orchids from crosses. The analysis was carried out through a direct morphological approach. The results obtained are hybrid orchids that have flowered from crosses that have flower morphology that is similar to the two parents of the cross, such as flower size, flower color, shape and color of the sepals and petals, and the color of the labellum depending on the characteristics of the flowers found in the two parents of the cross. The conclusion obtained in the form of the selection of the crossing parent is determined according to the desired flower character to produce a generation that has new flower variations and flowers from hybrid orchids produced from orchid breeding have a mixture of floral characteristics from the two orchid elders used as cross breeders, but not all flower characters. from the two elders who appeared, several flower characters emerged from one of the elders.
PENGARUH SUHU TERHADAP AKTIVITAS ANTIOKSIDAN DALAM DAUN ZAITUN (Olea europaea L.) DENGAN METODE DPPH Ulfi Abdul Rahman Oey; Tintrim Rahayu; Gatra Ervi Jayanti
Jurnal SAINS ALAMI (Known Nature) Vol 5, No 1 (2022)
Publisher : FMIPA UNISMA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/j.sa.v5i1.15927

Abstract

Causes of degenerative diseases are caused by activities and unhealthy lifestyle. unhealthy lifestyle which in turn triggers free radicals. free radicals are moleculs that are not oxidized which cause the formation of new molecules that can damage body cells. free radicals can be overcome by giving natural antioxidants, one of which is form the olive plant (Olea europaea L.).The purpose of this study was to determine the effect of temperature treatment on antioxidant activity in olive leaves based on leaf age by calculating the IC50 value. The method used in this research is the DPPH method. Based on the results of the study, it can be concluded that the temperature of 31.6°c in young leaves according to calculations is categorized as a strong antioxidant because it provides a calculated value of 82.778 ppm and 165.093 ppm on old leaves and classified as moderate antioxidants, while temperature of 44°c give dominant level under 31.6°c with IC50 values 123.78 ppm for young leaves and 165.170 ppm for old leaves and classified as moderate antioxidants according to the theory that compounds that have very strong antioxidant activity values IC50 values are less than 50 ppm, strong if the IC50 are between 50 and 100, moderate antioxidant if the IC50 values are feasible 100-250 ppm, weak if the IC50 value is 250-500 ppm and inactive if the IC50 value is more than 500 ppm. and vice versa related to the selection of leaf age also affects the level of antioxidant activity seen from the comparison of results from the two treatments that young leaves have a higher level of antioxidant activity than old leaves in counteracting free radicals.Keywords : Temperature, Antioxidant, Olive (Olea europaea L.), DPPH.ABSTRAKPenyebab penyakit degeneratif disebabkan karena aktivitas dan pola hidup yang kurang sehat. Pola hidup kurang sehat yang pada akhirnya memicu radikal bebas. Radikal bebas adalah molekul yang tidak teroksidasi yang menyebabkan terbentuk molekul baru yang dapat merusak sel tubuh. Radikal bebas dapat diatasi dengan pemberian antioksidan salah satunya dari tumbuhan zaitun (Olea europaea L.). Tujuan dari penelitian ini adalah untuk mengetahui pengaruh perlakuan suhu terhadap aktivitas antioksidan pada daun zaitun berdasarkan umur daun melalui perhitungan nilai IC50. Metode yang digunakan dalam penelitian ini adalah metode DPPH. Berdasarkan hasil penelitian dapat disimpulkan bahwa perlakuan suhu 31.6°C pada daun muda  menurut perhitungan IC50 dikategorikan sebagai antioksidan kuat karena memberikan nilai hasil perhitungan IC50  sebesar 82.778 ppm dan 165.093 ppm pada daun tua dan tergolong antioksidan sedang, sementara pada perlakuan suhu 44°C memberikan pengaruh lebih rendah dibandingkan dengan perlakuan suhu 31.6°C dengan nilai IC50 sebesar 123.78 ppm untuk daun muda dan 165.170 ppm untuk daun tua dan tergolong antioksidan sedang sesuai teori bahwa senyawa yang memiliki nilai aktivitas antioksidan sangat kuat jika nilai IC50 kurang dari 50 ppm, kuat apabila nilai IC50 bernilai antara 50 sampai 100, antioksidan sedang apabila jika nilai IC50 bernilai 100-250 ppm, lemah jika nilai IC50 bernilai 250-500 ppm dan tidak aktif jika nilai IC50 bernilai lebih dari 500 ppm, begitupun sebaliknya terkait pemilihan umur daun juga berpengaruh terhadap tinggi rendahnya aktivitas antioksidan dilihat dari perbandingan hasil dari kedua perlakuan bahwa daun muda memiliki tingkat aktivitas antioksidan lebih tinggi dibandingkan dengan daun tua dalam menangkal radikal bebas.Kata Kunci : Suhu, Antioksidan, Zaitun (Olea europaea L.), DPPH.
Indole-3-butyric acid immediately induced adventitious root of Dendrodium milla nayla x Dendrobium striaenopsis planted on coco-hust and wood charcoal Tintrim Rahayu; Gatra Ervi Jayanti; Dita Agisimanto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 28 No 1 (2022): December 2022
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/bphjbr.28.1.20226

Abstract

Orchids are one of the most favorite cut flowers, flowering potted plants, and have developed into a highly profitable industry. The development of acclimatization method is a necessity for the high survival rate of plantlets. The aim of this study was to investigate the effect of low indole-3-butyric acid (IBA) concentration on faster root formation of Dendrobium hybrid planted on coco- husk and charcoal in a short time. Plantlets derived from the seed culture of Dendrodium milla nayla x Dendrobium striaenopsis were harvested and pre-acclimated, planted on charcoal or coco-husk, and regularly sprayed with 0.25, 0.50, 0.75, and 1.0 mg/L IBA twice a week for one month. The results showed that IBA at 0.50 mg/L provided the most appropriate concentration for immediate root induction and growth of Dendrobium hybrid planted on coco-husk. Root number (1.75) and root length (0.28 cm) showed the highest and the most important indicator of adventitious root induction
Pengaruh Suhu terhadap Aktivitas Antioksidan dalam Daun Zaitun (Olea europaea L.) dengan Metode DPPH Ulfi Abdul Rahman Oey; Tintrim Rahayu; Gatra Ervi Jayanti
Jurnal SAINS ALAMI (Known Nature) Vol. 5 No. 1 (2022)
Publisher : FMIPA UNISMA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/j.sa.v5i1.15927

Abstract

Causes of degenerative diseases are caused by activities and unhealthy lifestyle. unhealthy lifestyle which in turn triggers free radicals. free radicals are moleculs that are not oxidized which cause the formation of new molecules that can damage body cells. free radicals can be overcome by giving natural antioxidants, one of which is form the olive plant (Olea europaea L.).The purpose of this study was to determine the effect of temperature treatment on antioxidant activity in olive leaves based on leaf age by calculating the IC50 value. The method used in this research is the DPPH method. Based on the results of the study, it can be concluded that the temperature of 31.6°c in young leaves according to calculations is categorized as a strong antioxidant because it provides a calculated value of 82.778 ppm and 165.093 ppm on old leaves and classified as moderate antioxidants, while temperature of 44°c give dominant level under 31.6°c with IC50 values 123.78 ppm for young leaves and 165.170 ppm for old leaves and classified as moderate antioxidants according to the theory that compounds that have very strong antioxidant activity values IC50 values are less than 50 ppm, strong if the IC50 are between 50 and 100, moderate antioxidant if the IC50 values are feasible 100-250 ppm, weak if the IC50 value is 250-500 ppm and inactive if the IC50 value is more than 500 ppm. and vice versa related to the selection of leaf age also affects the level of antioxidant activity seen from the comparison of results from the two treatments that young leaves have a higher level of antioxidant activity than old leaves in counteracting free radicals.Keywords : Temperature, Antioxidant, Olive (Olea europaea L.), DPPH.ABSTRAKPenyebab penyakit degeneratif disebabkan karena aktivitas dan pola hidup yang kurang sehat. Pola hidup kurang sehat yang pada akhirnya memicu radikal bebas. Radikal bebas adalah molekul yang tidak teroksidasi yang menyebabkan terbentuk molekul baru yang dapat merusak sel tubuh. Radikal bebas dapat diatasi dengan pemberian antioksidan salah satunya dari tumbuhan zaitun (Olea europaea L.). Tujuan dari penelitian ini adalah untuk mengetahui pengaruh perlakuan suhu terhadap aktivitas antioksidan pada daun zaitun berdasarkan umur daun melalui perhitungan nilai IC50. Metode yang digunakan dalam penelitian ini adalah metode DPPH. Berdasarkan hasil penelitian dapat disimpulkan bahwa perlakuan suhu 31.6°C pada daun muda  menurut perhitungan IC50 dikategorikan sebagai antioksidan kuat karena memberikan nilai hasil perhitungan IC50  sebesar 82.778 ppm dan 165.093 ppm pada daun tua dan tergolong antioksidan sedang, sementara pada perlakuan suhu 44°C memberikan pengaruh lebih rendah dibandingkan dengan perlakuan suhu 31.6°C dengan nilai IC50 sebesar 123.78 ppm untuk daun muda dan 165.170 ppm untuk daun tua dan tergolong antioksidan sedang sesuai teori bahwa senyawa yang memiliki nilai aktivitas antioksidan sangat kuat jika nilai IC50 kurang dari 50 ppm, kuat apabila nilai IC50 bernilai antara 50 sampai 100, antioksidan sedang apabila jika nilai IC50 bernilai 100-250 ppm, lemah jika nilai IC50 bernilai 250-500 ppm dan tidak aktif jika nilai IC50 bernilai lebih dari 500 ppm, begitupun sebaliknya terkait pemilihan umur daun juga berpengaruh terhadap tinggi rendahnya aktivitas antioksidan dilihat dari perbandingan hasil dari kedua perlakuan bahwa daun muda memiliki tingkat aktivitas antioksidan lebih tinggi dibandingkan dengan daun tua dalam menangkal radikal bebas.Kata Kunci : Suhu, Antioksidan, Zaitun (Olea europaea L.), DPPH.
Pengaruh Kolkisin Metode Semprot dan Tetes terhadap Respon Fenotipik dan Profil Kromosom Phalaenopsis pulcherrima Istiqomah, Nuri Lailatul; Rahayu, Tintrim; Jayanti, Gatra Ervi
Buletin Anatomi dan Fisiologi Volume 8, Nomor 2, Tahun 2023
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.8.2.2023.86-95

Abstract

Kolkisin  merupakan mutagen untuk memengaruhi karakter  Phalaenopsis pulcherrima  tipe bunga kecil, diharapkan bunga yang lebih besar saat fase generatif. Tujuan penelitian adalah mengamati pengaruh terbaik melalui metode dan konsentrasi kolkisin yang berbeda terhadap Phalaenopsis pulcherrima pada fase vegetatif. Penelitian dilakukan di  Laboratorium Orchidology dan Nursery, Universitas Islam Malang. Metode penelitian yang digunakan adalah metode eksperimen. Rancangan penelitian berupa rancangan Acak Lengkap (RAL) dengan tiga ulangan dan terdiri dari perlakuan tetes, semprot, dan kombinasi (tetes dan semprot). Konsentrasi kolkisin 0,5%, 1%, 1,5% dan 2%. Hasil uji multivariate dan uji univariat menunjukkan metode dan konsentrasi kolkisin yang berbeda berpengaruh pada respon fenotipik Phalaenopsis pulcherrima (diameter batang, diameter akar, luas daun, ketebalan daun serta berat basah tanaman). Pada uji lanjut Games-Howell 5% perlakuan tetes dengan konsentrasi 1% merupakan perlakuan kolkisin terbaik berdasarkan indikator diameter batang, luas daun serta berat tanaman dan perlakuan tetes dengan konsentrasi kolkisin 0,5% untuk indikator ketebalan daun. Perlakuan terbaik adalah tetes dan kombinasi (tetes dan semprot) dengan konsentrasi 2% terhadap diameter akar Phalaenopsis pulcherrima.   Colchicine is a mutagen to the affect character of Phalaenopsis pulcherrima’s small flower can be bigger flowers for the generative phase. The research was to observe the best effect of different methods and concentrations of colchicine on Phalaenopsis pulcherrima in the vegetative phase. The research was conducted in the Orchidology and Nursery Laboratory, Universitas Islam Malang. The experimental method is a method of this research. Research Design with completely randomized design (CRD) in three replications of drip, spray, and combination (drops and sprays) with concentrations of 0.5%, 1%, 1.5% and 2% of colchicine’s treatment. The multivariate and  univariate test showed different methods and concentrations of colchicine effected on phenotypic response of Phalaenopsis pulcherrima (stem diameter, root diameter, leaf area, leaf thickness, and plant weight). In Games-Howell test 5%, drip treatment and 1% concentration was the best colchicine treatment in indicators of stem diameter, leaf area, plant weight and 0.5% concentration in leaf thickness. Drops and combinations treatment (concentration of 2%) is best treatment of root diameter. The best treatments are drops and combinations (drops and sprays) with a concentration of 2% on the root diameter of Phalaenopsis pulcherrima.
Uji Beberapa Jenis Sitokinin Terhadap Pertumbuhan Protocorm Like Body (PLB) Anggrek (Dendrobium sp.) Pada Media MS Dalam Bentuk Thin Liquid Film Emeliya, Emeliya; Rahayu, Tintrim; Jayanti, Gatra Ervi; Agisimanto, Dita
Buletin Anatomi dan Fisiologi Volume 9, Nomor 1, Tahun 2024
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.9.1.2024.29-38

Abstract

Sitokinin berperan dalam pembelahan dan pembesaran sel sehingga memacu pertumbuhan tanaman,  sitokinin juga memacu pembentukan tunas baru. Tujuan penelitian ini adalah untuk mengetahui pengaruh pemberian berbagai jenis sitokinin terhadap pertumbuhan PLB Anggrek (Dendrobium sp.) pada media MS dalam bentuk Thin Liquid Film secara in vitro. Metode penelitian menggunakan penelitian eksperimental dengan rancangan acak Lengkap (RAL) yaitu perlakuan kontrol dan jenis sitokinin BAP 0,1 mg/L, Kinetin 0,1 mg/L, 2-iP 0,1 mg/L, Metatopolin 0,1 mg/L, dan Air kelapa 10 mL, sehingga terdapat 7 perlakuan dengan 4 kali ulangan. Parameter yang diamati adalah persentase eksplan tumbuh, respon eksplan, saat muncul tunas, akar pertama muncul, daun pertama muncul, warna daun dan berat PLB. Analisis data menggunakan ANNOVA. Berdasarkan hasil pengamatan didapatkan perlakuan terbaik pada perlakuan 2-iP 0,1 mg/L terhadap parameter respon PLB, waktu muncul tunas pertama dan waktu daun pertama muncul. Pada parameter waktu akar pertama muncul yang paling cepat pada perlakuan BAP 0,1 mg/L. Perubahan warna yang terjadi pada PLB yaitu hijau 145 B menjadi hijau 141 A pada perlakuan BAP 0,1 mg/L. Pada  perlakuan kinetin 0,1 mg/L merupakan berat PLB yang paling optimum.  Cytokinins play a role in cell division and enlargement thereby stimulating plant growth, cytokinins also stimulate the formation of new shoots. The aim of this research was to determine the effect of giving various types of cytokinins on the growth of PLB Orchids (Dendrobium sp.) on MS media in the form of Thin Liquid Film in vitro. The research method used experimental research with a completely randomized design (CRD), namely control treatment and cytokinin type BAP 0.1 mg/L, Kinetin 0.1 mg/L, 2-iP 0.1 mg/L, Metatopolin 0.1 mg/L L, and 10 mL coconut water, so there were 7 treatments with 4 repetitions. The parameters observed were the percentage of explant growth, explant response, when shoots appeared, first roots appeared, first leaves appeared, leaf color and PLB weight. Data analysis using ANNOVA. Based on the observation results, it was found that the best treatment was the 2-iP 0.1 mg/L treatment for the PLB response parameters, the time the first shoot appeared and the time the first leaf appeared. The time parameter for the first roots to appear was the fastest in the 0.1 mg/L BAP treatment. The color change that occurred in PLB was green 145 B to green 141 A in the BAP 0.1 mg/L treatment. In the kinetin treatment 0.1 mg/L was the most optimum PLB weight.
Korelasi Warna Ujung Akar dengan Warna Bunga Berdasarkan Anatomi Ujung Akar Anggrek Phalaenopsis, Dendrobium dan Vanda Alifia, Ersa; Rahayu, Tintrim; Jayanti, Gatra Ervi
Buletin Anatomi dan Fisiologi Volume 9, Nomor 1, Tahun 2024
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.9.1.2024.10-19

Abstract

Anggrek merupakan tanaman hias yang memiliki ciri khas bunga yang indah dan bentuk bunga yang membedakan dengan tanaman lainnya. Keragaman anggrek yang cukup tinggi di Indonesia, dibutuhkan penelitian untuk menunjukkan korelasi antara warna ujung akar dengan warna bunga anggrek, yang dapat dilihat dari anatomi ujung akar anggrek. Tujuan penelitian ini yaitu untuk mengetahui korelasi warna pada ujung akar dengan warna bunga anggrek  Phalaenopsis, Dendrobium dan Vanda. Penelitian ini menggunakan metode kualititatif secara deskriptif dengan menampilkan data dalam bentuk gambar dan deskripsi dari 9 sampel anggrek dengan warna yang berbeda, dan dianalisis dengan kuantitatif berdasarkan kode warna yang tertera pada Royal Horticultural Society (RHS) dikombinasikan dengan Red, Green, Blue (RGB), uji korelasi warna dilakukan menggunakan Korelasi Pearson. Hasil yang diperoleh pada penelitian ini menunjukkan warna ujung akar dengan warna bunga anggrek Phalaenopsis, Dendrobium dan Vanda terdapat adanya pigmen pembentuk warna klorofil, antosianin dan karotenoid yang dapat dilihat dari anatomi ujung akar pada sel korteks. Vanda memiliki nilai korelasi yang diperoleh sebesar 0,661 dan dinyatakan mempunyai kesamaan hubungan positif dengan kekuatan korelasi paling kuat dibandingkan anggrek Phalaenopsis dan Dendrobium.  Kesimpulan, yaitu warna ujung akar dengan bunga anggrek Phalaenopsis, Dendrobium dan Vanda memiliki korelasi warna yang dapat dilihat dari anatomi ujung akar irisan melintang pada sel korteks.  Orchids are ornamental plants that have beautiful flower characteristics and flower shapes that distinguish them from other plants. The diversity of orchids is quite high in Indonesia, research is needed to show the correlation between the color of the root tip and the color of the orchid flower, which can be seen from the anatomy of the root tip of the orchid. The purpose of this study was to determine the correlation of the color at the tip of the root with the color of the Phalaenopsis, Dendrobium and Vanda orchids. This study used a descriptive qualitative method by displaying data in the form of pictures and descriptions of 9 samples of orchids with different colors, and analyzed quantitatively based on the color codes listed on the Royal Horticultural Society (RHS) combined with Red, Green, Blue (RGB), color correlation test was performed using Pearson Correlation. The results obtained in this study showed that the color of the root tips with the colors of Phalaenopsis, Dendrobium and Vanda orchids contained pigments that form the color of chlorophyll, anthocyanins and carotenoids which can be seen from the anatomy of the root tips in the cortical cells. Vanda has a correlation value of 0.661 and is stated to have the same positive relationship with the strongest correlation strength compared to Phalaenopsis and Dendrobium orchids. The conclusion is that the color of the root tip with Phalaenopsis, Dendrobium and Vanda flowers has a color correlation which can be seen from the anatomy of the root tip of the cross section of the cortex cells.
Analisis In Silico dan Aktivitas Antioksidan Senyawa Nano Kompleks Pada Daun dan Cabang Bidara (Ziziphus mauritiana Lamk ): In Silico Analysis and Antioxidant Activity of Nano Complex Compounds on Leaves and Branches of Bidara (Ziziphus mauritiana Lamk) Nisa, Kholisatun; Rahayu, Tintrim; Jayanti, Gatra Ervi
Jurnal SAINS ALAMI (Known Nature) Vol. 6 No. 1 (2023)
Publisher : FMIPA UNISMA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/j.sa.v6i1.13077

Abstract

Bidara (Ziziphus mauritiana Lamk.) is a source of natural antioxidants that contain antioxidant compounds such as flavonoids, carotenoids, phenolic, and ascorbic acid. Antioxidants in this study were in the form of complex compounds without taking any of the compounds (single compounds). In addition to complex compounds, bidara with nano size can play a maximum role in carrying out its function as an antioxidant in the body. This study aims to determine the composition of active compounds that act as antioxidants in leaves, branches, and combinations of bidara leaves through in silico analysis and to determine the effectiveness of antioxidant tests on nano complex compounds of leaves, branches, and combinations of bidara leaves. In silico analysis of active compounds was carried out online using the Dr. website. Duke's Phytochemical and Ethnobotanical Databases, Passonline, Smiles and Stitch. Experimental research using freeze dry so that bidara becomes a nanometer-sized powder. The samples used were leaves, branches and a combination of leaves and branches tested by the 2,2 Diphenyl 1 picrylhydrazyl (DPPH) method. The results of the in silico analysis showed that there are 4 compounds in the bidara plant that act as antioxidants, namely caumarin and rutin in the leaves, batulinic-acid and ceanothic-acid in the branches. The experimental results showed that the antioxidant activity of the three treatments had differences in antioxidant effectiveness. The color change of DPPH indicates the presence of antioxidants, the color of the leaf sample from dark purple to yellow, the color of the branch sample from dark purple to light purple, and the sample of the combination of leaves from dark purple to yellowish cloudy white. The color change indicates that the combination of leaves and branches has high antioxidant activity, then leaves and finally branches. Microscopic observations showed the formation of crystals in the bidara powder. The crystal structure shows an orderly and organized pattern. The active compounds in bidara that act as antioxidants are caumarin, rutin, batulinic-acid, and ceanothic-acid. The combination of leaves and branches has a high antioxidant activity compared to leaves and branches. Keywords: bidara, In silico, complex compound, antioxidant activity.   ABSTRAK Bidara (Ziziphus mauritiana Lamk.) merupakan sumber antioksidan alami yang memiliki kandungan senyawa antioksidan seperti flavonoid, karotenoid, fenolik, dan asam askorbat. Antioksidan dalam penelitian ini berupa senyawa kompleks tanpa mengambil salah satu senyawanya (senyawa tunggal). Selain senyawa kompleks, bidara dengan ukuran nano dapat berperan maksimal dalam menjalankan fungsinya sebagai antioksidan di dalam tubuh. Tujuan penelitian ini untuk mengetahui komposisi senyawa aktif yang berperan sebagai antioksidan pada daun, cabang, dan kombinasi daun-cabang bidara melalui analisis in silico serta untuk mengetahui efektivitas dari uji antioksidan pada senyawa nano kompleks daun, cabang, serta kombinasi daun-cabang bidara. Analisis senyawa aktif melalui in silico dilakukan secara online dengan menggunakan website Dr. Duke’s Phytochemical and Ethnobotanical Databases, Passonline, Smiles dan Stitch. Penelitian eksperimental dengan menggunakan freeze dry sehingga bidara menjadi serbuk berukunan nanometer. Sampel yang digunakan adalah daun, cabang dan kombinasi daun-cabang dan diuji dengan metode 2,2 Diphenyl 1 picrylhydrazyl (DPPH). Hasil analisis in silico terdapat 4 senyawa dalam tanaman bidara yang memiliki peran sebagai antioksidan yaitu caumarin dan rutin pada daun, batulinic-acid dan ceanothic-acid pada cabang. Hasil penelitian eksperimantal menunjukkan bahwa aktivitas antioksidan dari tiga perlakuan memiliki perbedaan efektivitas antioksidan. Perubahan warna DPPH menunjukkan adanya antioksidan, warna pada sampel daun dari ungu pekat menjadi kuning, warna pada sampel cabang dari ungu pekat menjadi ungu muda, dan sampel kombinasi daun-cabang dari ungu pekat menjadi putih keruh kekuningan. Perubahan warna tersebut menunjukkan bahwa kombinasi daun-cabang memiliki aktivitas antioksidan tinggi, kemudian daun dan terakhir cabang. Pada pengamatan mikroskop menunjukkan terbentuknya kristal dalam serbuk bidara. Struktur kristal menunjukkan pola yang teratur dan terorganisir. Senyawa aktif dalam bidara yang berperan sebagai antioksidan adalah caumarin, rutin, batulinic-acid dan ceanothic-acid. Kombinasi daun-cabang mempunyai aktivitas antioksidan yang tinggi dibandingkan daun dan cabang. Kata kunci: bidara, In silico, senyawa kompleks, aktivitas antioksidan.
Co-Authors Achyar, Zhahrotun Khofifah Nur Alifia, Ersa Ari Hayati Asari, Devi Indrayani Fitri Azizi, Rosita Imro’atul Dewi, Lina Purnama Dita Agisimanto Dita Agisimanto Emeliya, Emeliya Fitri, Hanin Rahma Hadi, Muhammad Sholikhul Haikal, Muhammad Wildan Istifadah, Hayati Istiqomah, Nuri Lailatul Jamilah, Zahrotul Ma'rifah, Shofia Jannatul Marista, Bilqis Mawardi, Azis Muhammad Ni'amul Albab Mustakim, Moch Muzayyan, Abdullah Rozzen Nindi Afifa Nisa Nisa, Kholisatun Nur Azizah Nur Azizah Nurul Jadid Mubarokati Oey, Ulfi Abdul Rahman Paramitha, Armanda Bella Prasetyowati, Eka Purnamasari, Viska Puspa, Andria Putri, Nabiilah Rifda Harmono Putri, Nela Vede Rhofa Rafida Azizah Rafsanjani, Ramizard Ramadhan, Majida Reza Priski Dwi Jayanti Safitri, Dewi Astriana Salsabilla, Dyah Ajeng Saraswati Subagjo, Saraswati Setyowati, Dewi Anjar Syafitri, Fitria Indah Syarivah, Thoifatus Tintrim Rahayu, Tintrim Ulfi Abdul Rahman Oey Vikriansyah, Muchammad Faruq