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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Ilmu Dasar BERKALA SAINSTEK Biology, Medicine, & Natural Product Chemistry Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Jurnal Sains Alami (Known Nature) Berkala Penelitian Hayati Biopedagogia Jurnal Sains Alami (Known Nature) Exhibition and Seminar on Science and Creative Technology – Al Azhar Proceeding
Gatra Ervi Jayanti
Biology Department, Faculty Of Mathematics And Natural Sciences, Universitas Islam Malang, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Education Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Other

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Pengaruh Suhu terhadap Aktivitas Antioksidan dalam Daun Zaitun (Olea europaea L.) dengan Metode DPPH Oey, Ulfi Abdul Rahman; Rahayu, Tintrim; Jayanti, Gatra Ervi
Jurnal SAINS ALAMI (Known Nature) Vol. 5 No. 1 (2022)
Publisher : FMIPA UNISMA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/j.sa.v5i1.15927

Abstract

Causes of degenerative diseases are caused by activities and unhealthy lifestyle. unhealthy lifestyle which in turn triggers free radicals. free radicals are moleculs that are not oxidized which cause the formation of new molecules that can damage body cells. free radicals can be overcome by giving natural antioxidants, one of which is form the olive plant (Olea europaea L.).The purpose of this study was to determine the effect of temperature treatment on antioxidant activity in olive leaves based on leaf age by calculating the IC50 value. The method used in this research is the DPPH method. Based on the results of the study, it can be concluded that the temperature of 31.6°c in young leaves according to calculations is categorized as a strong antioxidant because it provides a calculated value of 82.778 ppm and 165.093 ppm on old leaves and classified as moderate antioxidants, while temperature of 44°c give dominant level under 31.6°c with IC50 values 123.78 ppm for young leaves and 165.170 ppm for old leaves and classified as moderate antioxidants according to the theory that compounds that have very strong antioxidant activity values IC50 values are less than 50 ppm, strong if the IC50 are between 50 and 100, moderate antioxidant if the IC50 values are feasible 100-250 ppm, weak if the IC50 value is 250-500 ppm and inactive if the IC50 value is more than 500 ppm. and vice versa related to the selection of leaf age also affects the level of antioxidant activity seen from the comparison of results from the two treatments that young leaves have a higher level of antioxidant activity than old leaves in counteracting free radicals.Keywords : Temperature, Antioxidant, Olive (Olea europaea L.), DPPH.ABSTRAKPenyebab penyakit degeneratif disebabkan karena aktivitas dan pola hidup yang kurang sehat. Pola hidup kurang sehat yang pada akhirnya memicu radikal bebas. Radikal bebas adalah molekul yang tidak teroksidasi yang menyebabkan terbentuk molekul baru yang dapat merusak sel tubuh. Radikal bebas dapat diatasi dengan pemberian antioksidan salah satunya dari tumbuhan zaitun (Olea europaea L.). Tujuan dari penelitian ini adalah untuk mengetahui pengaruh perlakuan suhu terhadap aktivitas antioksidan pada daun zaitun berdasarkan umur daun melalui perhitungan nilai IC50. Metode yang digunakan dalam penelitian ini adalah metode DPPH. Berdasarkan hasil penelitian dapat disimpulkan bahwa perlakuan suhu 31.6°C pada daun muda  menurut perhitungan IC50 dikategorikan sebagai antioksidan kuat karena memberikan nilai hasil perhitungan IC50  sebesar 82.778 ppm dan 165.093 ppm pada daun tua dan tergolong antioksidan sedang, sementara pada perlakuan suhu 44°C memberikan pengaruh lebih rendah dibandingkan dengan perlakuan suhu 31.6°C dengan nilai IC50 sebesar 123.78 ppm untuk daun muda dan 165.170 ppm untuk daun tua dan tergolong antioksidan sedang sesuai teori bahwa senyawa yang memiliki nilai aktivitas antioksidan sangat kuat jika nilai IC50 kurang dari 50 ppm, kuat apabila nilai IC50 bernilai antara 50 sampai 100, antioksidan sedang apabila jika nilai IC50 bernilai 100-250 ppm, lemah jika nilai IC50 bernilai 250-500 ppm dan tidak aktif jika nilai IC50 bernilai lebih dari 500 ppm, begitupun sebaliknya terkait pemilihan umur daun juga berpengaruh terhadap tinggi rendahnya aktivitas antioksidan dilihat dari perbandingan hasil dari kedua perlakuan bahwa daun muda memiliki tingkat aktivitas antioksidan lebih tinggi dibandingkan dengan daun tua dalam menangkal radikal bebas.Kata Kunci : Suhu, Antioksidan, Zaitun (Olea europaea L.), DPPH.
Pengaruh variasi konsentrasi indole butyric acid (iba) pada anggrek (dendrobium hybrid) terhadap pertumbuhan dan survival dalam media cocopeat: Effect of indole butyric acid (iba) concentration variations on orchid (dendrobium hybrid) on growth and survival in cocopeat media Setyowati, Dewi Anjar; Rahayu, Tintrim; Jayanti, Gatra Ervi; Agisimanto, Dita
Jurnal SAINS ALAMI (Known Nature) Vol. 5 No. 2 (2023)
Publisher : FMIPA UNISMA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/j.sa.v5i2.16959

Abstract

Orchids have endemic flowers with high aesthetic value and are widespread throughout Indonesia. One of the widely cultivated orchid varieties is the Dendrobium Orchid. The orchid cultivation process requires special handling, especially in the growing environment, which must be by the plant’s original habitat, so that plant growth and development become more optimal, especially the planting medium and the provision of nutrients must be appropriate. This study aimed to determine the optimal concentration of IBA on the growth of orchid plants (Dendrobium hybrid) and to determine the optimal variation of IBA concentration on the survival of orchid plants in cocopeat media. The research method was carried out experimentally using the Randomized Block Design (RAK). To compare the growth between the concentration of IBA and root growth has given four treatments: P1: 0.25 ml/L, P2: 0.50 ml/L, P3: 0, 75ml/L, P4: 1ml/L each treatment was given three replications and each replication contained four plant seeds with a total of 48 plant seeds. Observation parameters included root length, number of new roots, leaf length, number of leaves, plant length, plant wet weight, and plant survival. The optimal concentration of IBA on orchid growth at a concentration of 0.25 ml/L IBA optimal orchid growth was found in the parameters of the number of new roots. Plant length at a concentration of 0.75 ml/L IBA optimal orchid growth was found in root length and planted wet weight parameters. In the survival parameters of orchid plant life, the optimal concentration of IBA was found at a concentration of 0.25 ml/L.  Keywords: Orchid, growth, orchid survival, and IBA   ABSTRAK Anggrek memiliki bunga endemik yang memiliki nilai estetika tinggi dan menyebar luas di seluruh Indonesia. Salah satu varietas anggrek yang banyak dibudidayakan yaitu Anggrek Dendrobium. Proses budidaya anggrek memerlukan penanganan khusus terutama pada lingkungan tumbuh yang harus sesuai dengan habitat asli tanaman, agar pertumbuhan dan perkembangan tanaman menjadi lebih optimal terutama media tanam dan pemberian unsur hara harus tepat. Tujuan penelitian ini adalah untuk mengetahui konsentrasi pemberian IBA yang optimal terhadap pertumbuhan tanaman angrek (Dendrobium hybrid) serta untuk mengetahui pemberian variasi konsentrasi IBA yang optimal terhadap survival kehidupan tanaman anggrek dalam media cocopeat. Metode penelitian dilakukan secara eksperimental dengan menggunakan Rancangan Acak Kelompok (RAK), untuk membandingkan pertumbuhan antara konsentrasi IBA terhadap pertumbuhan akar yang diberi empat perlakuan P1: 0,25 ml/L, P2: 0,50 ml/L, P3: 0,75ml/L, P4: 1ml/L masing-masing perlakuan di berikan tiga ulangan dan setiap ulangan terdapat empat bibit tanaman dengan jumlah keseluruhan 48 bibit tanaman. Parameter pengamatan meliputi panjang akar, jumlah akar baru, panjang daun, jumlah daun, panjang tanaman, berat basah tanaman, dan survival tanaman. Konsentrasi IBA yang optimal terhadap pertumbuhan anggrek pada konsentrasi 0,25 ml/L IBA, pertumbuhan anggrek yang optimal terdapat pada parameter jumlah akar baru. Pada konsentrasi 0,50 ml/L IBA pertumbuhan anggrek yang optimal terdapat pada parameter panjang daun, jumlah daun, dan panjang tanaman. Pada konsentrasi 0,75 ml/L IBA pertumbuhan anggrek yang optimal terdapat pada parameter panjang akar dan berat basah tanaman. Pada parameter survival kehidupan tanaman anggrek konsentrasi pemberian IBA yang optimal terdapat pada konsentrasi 0,25 ml/L. Kata kunci: Anggrek, pertumbuhan, survival anggrek, dan IBA
Kajian Akar Kadaka sebagai Media Tanam dengan Pengaruh Variasi Konsentrasi Indole Butyric Acid Tterhadap Pertumbuhan Anggrek Dendrobium canaliculatum: Study of Kadaka Roots as a Plant Media with the Effect of Variation Concentrations of Indole Butyric Acid on Dendrobium canaliculatum Orchid Growth Hadi, Muhammad Sholikhul; Rahayu, Tintrim; Jayanti, Gatra Ervi; Agisimanto, Dita
Jurnal SAINS ALAMI (Known Nature) Vol. 6 No. 1 (2023)
Publisher : FMIPA UNISMA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/j.sa.v6i1.17140

Abstract

Dendrobium is an orchid that many orchid lovers like. Efforts to increase the quantity of orchids in the growth phase can be done in several ways, namely through the regulation of fertilization and the use of appropriate planting media. Planting media from kadaka roots is the right media for the growth of orchid. The addition of nutrients N, P, and K together showed a significant increase in total biomass. In addition to fertilizers, growth regulators substances also affect growth. The plant hormone used in plant cultivation is the auxin hormone. One type of auxin hormone type is Indole Butyric Acid (IBA) which functions to stimulate root formation. This study aimed to study the effect of IBA concentration on the root growth of Dendrobium orchids grown on kadaka root media. Dendrobium orchids seedlings were selected beforehand, planted in kadaka roots, and treated twice a week. The treatment was carried out for two months using IBA concentrations of 0.25, 0.50, 0.75, and 1.0 ml/L. The administration of IBA hormone on the growth of Dendrobium canaliculatum affects the number of new roots, leaf length, and plant height. IBA at 0.25 ml/L was the optimal concentration for the number of new roots. The number of new roots as an indicator of optimal orchid growth in response to IBA hormone administration.  Keywords: Kadaka, Indole Butyric Acid (IBA), Orchid, Orchid Growth   ABSTRAK Dendrobium adalah anggrek yang banyak disukai pecinta anggrek. Upaya peningkatan kuantitas anggrek pada fase pertumbuhan dapat dilakukan dengan beberapa cara yaitu melalui pengaturan pemupukan serta penggunaan media tanam yang sesuai. Media tanam dari akar kadaka merupakan media yang tepat untuk pertumbuhan anggrek. Penambahan unsur hara N, P, dan K pada media secara bersama menunjukkan peningkatan total biomassa yang signifikan. Selain pupuk, Zat Pengatur Tumbuh (ZPT) juga berpengaruh terhadap pertumbuhan. Hormon tanaman yang dipergunakan pada pembudidayaan tanaman adalah hormon auksin. Salah satu jenis hormon auksin merupakan Indole Butyric Acid (IBA) yang dipergunakan untuk merangsang pembentukan akar. Penelitian ini bertujuan untuk mengkaji efek konsentrasi IBA terhadap pertumbuhan akar anggrek Dendrobium yang ditanam pada media akar kadaka. Tanaman anggrek yang digunakan adalah seedling Dendrodium, dilakukan seleksi, ditanam di akar kadaka, dan diberi perlakuan dua kali seminggu. Perlakuan dilakukan selama dua bulan menggunakan konsentrasi IBA 0,25, 0,50, 0,75, dan 1,0 ml/L. Pemberian hormon IBA terhadap pertumbuhan anggrek Dendrobium canalicullatum berpengaruh pada jumlah akar baru, panjang daun dan tinggi tanaman. IBA pada 0,25 ml/L merupakan konsentrasi yang optimal untuk jumlah akar baru. Jumlah akar baru sebagai indikator pertumbuhan anggrek yang optimal terhadap respon pemberian hormon IBA.  Kata kunci: Kadaka, Indole Butyric Acid (IBA), Anggrek, Pertumbuhan
Pengaruh Ekstrak Kulit Umbi Bawang Merah (Allium cepa) dan Bawang Putih (Allium sativum) untuk Pertumbuhan Anggrek Dendrobium: The Effect of Shallot (Allium cepa) and Garlic (Allium sativum) Skin Extracts on Dendrobium Orchid Growth Marista, Bilqis; Rahayu, Tintrim; Jayanti, Gatra Ervi
Jurnal SAINS ALAMI (Known Nature) Vol. 6 No. 2 (2024)
Publisher : FMIPA UNISMA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/j.sa.v6i2.18809

Abstract

Genetic and physiological factors influence dendrobium orchids growth and flowering. In maintaining Dendrobium orchids, the cultivation process after acclimatization greatly affects growth and development, so its quality and sustainability can be threatened. Onion bulb skin and garlic bulb skin were able to increase germination and growth variables. This research aims to determine the effect of the most effective concentration on the growth of Dendrobium orchids. This research method used an experimental method with a completely randomized design (CRD) with 24 plants, research parameters included the number of new shoots, number of new shoots, number of new roots, new root length, leaf length, total number of leaves, plant height, and weight. Plants were analyzed descriptively, and multivariate correlation tests were using IBM software. The results showed that the M40: P20 treatment was the best treatment with parameters that responded, namely the number of new shoots, length of new roots, length of leaves, total number of leaves and plant height. This shows that the administration of shallot bulb skin extract and garlic bulb skin extract positively affected several parameters. Keywords : acclimatization, Dendrobium, effectiveness, parameters, plants.   ABSTRAK Anggrek Dendrobium dalam pertumbuhan dan pembungaan dipengaruhi faktor genetik dan faktor fisiologis. Dalam pemeliharaan anggrek Dendrobium proses budidaya setelah aklimatisasi sangat mempengaruhi pertumbuhan dan perkembangan, sehingga kualitas dan kelestraiannya dapat terancam. Kulit umbi bawang merah dan kulit umbi bawang putih mampu meningkatkan perkecambahan dan variabel pertumbuhan. Tujuan penelitian ini yaitu untuk mengetahui pengaruh dari konsentrasi yang paling efektif terhadap pertumbuhan anggrek Dendrobium. Metode penelitian menggunakan eksperimental Rancangan Acak Lengkap (RAL) dengan 24 tanaman, parameter penelitian meliputi jumlah tunas baru, jumlah daun tunas baru, jumlah akar baru, panjang akar baru, panjang daun, total jumlah daun, tinggi tanaman, dan berat kering tanaman yang dianalisis secara deskriptif dan uji korelasi multivariat test menggunakan software IBM. Hasil penelitian menunjukkan perlakuan M40: P20 adalah perlakuan terbaik dengan parameter yang merespon yaitu jumlah daun tunas baru, panjang akar baru, panjang daun, jumlah total daun dan tinggi tanaman. Hal ini menunjukkan bahwa pemberian ekstrak kulit umbi bawang merah dan kulit umbi bawang putih memberikan pengaruh positif pada beberapa perameter. Kata kunci: aklimatisasi, Dendrobium, efektif, parameter, tanaman.
The Effect of the Type of Planting Media and The Addition of O2 Nanobubbles on the Growth of Dendrobium burana Green × Ong Ang Ai Boon Orchid Plantlets in Vitro Purnamasari, Viska; Rahayu, Tintrim; Jayanti, Gatra Ervi; Agisimanto, Dita
Jurnal ILMU DASAR Vol 25 No 1 (2024)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v25i1.41376

Abstract

Dendrobium orchids come in a variety of colors, shapes, and beauty that are in great demand. The process of growing orchids requires special handling of an appropriate growing environment, such as proper planting media and nutrients that can stimulate orchid growth. This type of thin liquid film media is the use of liquid media with an immersion system while vermiculite and perlite media have almost the same properties. However, vermiculite has a higher water absorption capacity than perlite. Nanobubbles are bubbles with 1-100 nm that can live more stably in water. This study aims to influence the type of media and the addition of Nanobubbles O2 on the growth of Dendrobium burana Green × Ong Ang Ai Boon orchid plantlets. This research was carried out from November to December 2022 at the PT. Java Indo Arjuna Singosari, Malang. This study used a completely randomized design (CRD) with 10 treatments, namely solid MS, gaviota solid, thin liquid film, thin liquid film + NBs O2, vermiculite, vermiculite + NBs O2, perlite, perlite + NBs O2, vermiculite + Perlite, vermiculite + perlite + NBs O2 with 4 replications. The data were analyzed descriptively and the Manova test. The results showed that the vermiculite + NBs O2 treatment had the potential to increase the length of plantlets by 13.80 mm, the wet weight of plantlets by 0.30 g, the percentage of live plantlets by 100% whereas in the vermiculite + perlite treatment has the potential for the first root growth to appear, namely 5 HST. The vermiculite treatment had the potential to increase the highest number of leaves by 5 leaves, whereas in the leaf color treatment the solid MS treatment had a more intense green color.
Potential of Ecoenzymes and N2 Nanobubbles on the Growth of Phalaenopsis sp. Orchid at the Acclimatization Stage Syafitri, Fitria Indah; Rahayu, Tintrim; Jayanti, Gatra Ervi
Jurnal ILMU DASAR Vol 25 No 1 (2024)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v25i1.40575

Abstract

Growth is a quantitative change during one plant’s life cycle and is irreversible.withaddition An in crease the volume or dry weight of plants or other organs is due to the adding, of new structural elements. Phalaenopsis (moth orchid) is a genus of orchids appeal. The diversity of colors, shapes, and textures, as well as its aroma, make Phalaenopsis orchids one of the charming flowers of Indonesia. Ecoenzyme is a solution of complex organic substances produced from the fermentation of organic residues, sugar, and water. Nanobubbles (NBs) are an example of nanotechnology. NBs have a diameter of 1-100 nm and are nanoscopic gas bubbles in a solution or water that can change the characteristics of water and are stable. This study aims to determine the potential of giving Ecoenzyme and NBs N2 on the growth of Phalaenopsis orchids during the acclimatization stage. This research was conducted from October to December 2022 at the Orchidology and Nursery Laboratory, Islamic University of Malang. This study used an experimental method with a completely randomized design (CRD) with 10 treatments, namely 0 mL/L (control), 1 mL/L Ecoenzyme, 2 mL/L Ecoenzyme, 3 mL/L Ecoenzyme, 4 mL/L Ecoenzyme, 4 combination NBs N2 and Ecoenzymes, and 5 mL NBs N2 with 3 replications, making a total of 30 treatment units. The research parameters included the number of leaves, leaf length, number of roots, root length, plant height, fresh weight, and plant dry weight. The results of this study showed that the treatment of 3 mL/L Ecoenzyme showed potential in increasing the number of leaves by 4 pieces: at the same time the optimal concentration of the combination of Ecoenzyme and NBs was 2 mL/L plus 5 mL NBs N2 in increasing the number of roots by 10 pieces, and the optimal concentration of NBs was 5 mL in increasing the root length by 7.07 cm, plant height by 17.10 cm leaf. length, plant weight by 4.16 g and dry weight by 0.85 g in Phalaenopsis orchids.
Analysis of Nanobubbles (NBs) Technology and Foliar Fertilization on the Growth of Phalaenopsis sp. Orchid Puspa, Andria; Rahayu, Tintrim; Jayanti, Gatra Ervi
BERKALA SAINSTEK Vol 12 No 2 (2024)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v12i2.39809

Abstract

Plants need nutrients which are then provided through fertilizers, but this can cause long-term problems due to limited absorption by plants so recently implemented NBs technology that can help reduce environmental pollution. This study aimed to determine the effect of NBsO2, NBsH2, NBsCO2, NBsN2 and, NBsO3 on the growth of Phalaenopsis sp. orchid plants. The research method was a complete randomized design (RAL) with 7 treatments (control without application of fertilizer and NBs, foliar fertilizer, NBsO2, NBsH2, NBsCO2, NBsN2, NBsO3) and 4 repeats. NBs were made using a nanogenerator with a flow of 5 liters/ minute and induced for 15 minutes. The data analysis used is the Multivariate Analysis of Variance (MANNOVA) test. The control and NBs treatments were given by dropping 5 ml on each orchid plant. The results showed that leaf length, number of leaves, plant weight and number of plant roots NBsN2 treatment had the best effect, while on root length, NBs O2 treatment had a better effect than other treatments. The application of foliar fertilizer is less effective to meet plant growth needs. At the same time, NBs are more effective because they are very small making it easier for plants to absorb.
The New Technology for In Vitro Culture with Induction of Nanobubbles (NBsN2 and NBsO2) in Cattleya sp. Mustakim, Moch; Mawardi, Azis; Dewi, Lina Purnama; Putri, Nela Vede Rhofa; Rahayu, Tintrim; Jayanti, Gatra Ervi
Biology, Medicine, & Natural Product Chemistry Vol 13, No 2 (2024)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2024.132.459-465

Abstract

The growth and development of orchids take a relatively long time, while the demand for high-quality orchids continues to increase significantly. Tissue culture is a technique of isolating plant parts in organs, tissues, and cells and then culturing these plants on artificial media in a sterile environment. Tissue culture techniques can reproduce plants in a relatively short time, with the same properties and quality, so efforts to optimize tissue culture with technology are the right solution, one of which is using nanogenerator technology that produces Nanobubbles (NBs). NBs are one of the nanotechnologies that are ?100 nm in size with induced gas needed by plants. This study aims to determine the effectiveness of growth and development from the induction of NBsN2 and NBsO2 in Cattleya sp. planlet in vitro culture. This research method uses in vitro culture with a Randomized Group Design. The results of the MsO treatment showed higher weight than other treatments, with a total plant weight value of 0.3550 g and a total plant height value of 1.3983 cm. Murashige-Skoog + NBsNitrogen (MsN) treatment showed higher root length than other treatments with a total value of 1.2367 cm. In the treatment of Ms, MsO, OJ and NJ, a live percentage of 83-100% was observed. The statistical test results indicate that NBs positively affect Cattleya sp. plantlets. This effect includes increasing plant weight, height, and root length and promoting new shoots growth. Additionally, nitrogen supplements play a role in colour changes, indicating the plant's health and photosynthetic efficiency. In the treatment with Induction O2, the initial colour changes from Strong Yellow Green (141 D) to Strong Yellowish Green (141 C), while in the treatment with Induction NBsN2, the initial colour changes from Strong Yellow Green (141 D) to Deep Yellow Green (141 B).
Pengaruh Spektrum Warna dan Penambahan Nanobubbles (NBs) Nitrogen (N2) terhadap Pertumbuhan Anggrek Dendrobium sp. secara In Vitro: The Influence of Color Spectrum and Addition of Nitrogen (N2) Nanobubbles (NBs) on the In Vitro Growth of Dendrobium sp. Orchids Salsabilla, Dyah Ajeng; Rahayu, Tintrim; Jayanti, Gatra Ervi
Jurnal SAINS ALAMI (Known Nature) Vol. 8 No. 1 (2025)
Publisher : FMIPA UNISMA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/j.sa.v8i1.18811

Abstract

In in vitro culture, light and nitrogen absorption play crucial roles in the growth of Dendrobium sp orchids. Light affects plant absorption; thus, nitrogen must be added in the right amount for optimal growth. Nanobubbles (NBs) technology has been developed to convert nitrogen into smaller particles, facilitating plant absorption. This study aims to investigate the effects of color spectrum and N2 NBs on the growth of Dendrobium sp orchids. The experimental research method involved different treatments of media (MS; MS+ZPT; MS+ NBs N2; MS+ZPT+NBs N2) and color spectrum (white, red, blue, red-blue). Data on live percentage, plantlet legth and plantlet weight were analyzed descriptively and statistically (normality, homogeneity, multivariate, and Games-Howell tests). The results showed that the highest percentage of live plantlets, 97.91%, was found in the treatments with red and red-blue color spectrum. The red-blue color spectrum significantly increased the plantlet weight by 0.363 g in the MS treatment, and the application of red color spectrum potentially increased the plantlet length by 1.707 mm in the MS+ZPT treatment. The addition of NBs N2 in the subculture media showed potential for 100% live plantlet percentage in the MS+NBs N2 treatment. Keywords: Dendrobium, nanobubbles (NBs), color spectrum, in vitro, Nitrogen ABSTRAK Dalam kultur in vitro, penyerapan cahaya dan nitrogen berperan penting terhadap pertumbuhan anggrek Dendrobium sp. Cahaya mempengaruhi penyerapan tanaman, sehingga nitrogen harus ditambahkan dalam jumlah yang tepat untuk pertumbuhan optimal. Teknologi Nanobubbles (NBs) telah dikembangkan untuk mengubah nitrogen menjadi partikel yang lebih kecil agar memudahkan penyerapan oleh tanaman. Penelitian ini bertujuan untuk mengetahui pemberian spektrum warna dan NBs  terhadap pertumbuhan anggrek Dendrobium sp. Metode penelitian eksperimental dengan perlakuan media (MS; MS ₊ ZPT; MS ₊ NBs ; MS ₊ ZPT ₊ NBs ) dan spektrum warna (putih, merah, biru, merah-biru). Data persentase hidup, panjang planlet dan berat planlet dianalisis secara deskriptif dan uji statistik (normalitas, homogenitas, multivariat, dan Games Howell). Hasil penelitian menunjukkan bahwa pada persentase hidup planlet tertinggi yaitu 97,91% terdapat pada perlakuan spektrum warna merah dan merah-biru. Spektrum warna merah-biru berpengaruh dalam meningkatkan berat planlet sebesar 0,363 g pada perlakuan MS dan pemberian spektrum warna merah berpotensi meningkatkan panjang planlet sebesar 1,707 mm pada perlakuan MS + ZPT. Dalam pemberian NBs  dalam media subkultur memberikan potensi persentase hidup planlet sebesar 100% pada perlakuan MS ₊ NBs .  Kata kunci: Dendrobium, Nanobubbles (NBs), Spektrum warna, Nitrogen
Profil Senyawa Metabolit Sekunder dan Deteksi Antosianin pada Buah Stroberi (fragaria x ananassa) Menggunakan Kromatografi Lapis Tipis dan Spektrofotometri Inframerah: Profile of Secondary Metabolite Compounds and Anthocyanin Detection in Strawberry Fruit (Fragaria x ananassa) Using Thin-Layer Chromatography and Infrared Spectrophotometry Haikal, Muhammad Wildan; Rahayu, Tintrim; Jayanti, Gatra Ervi
Jurnal SAINS ALAMI (Known Nature) Vol. 8 No. 1 (2025)
Publisher : FMIPA UNISMA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/j.sa.v8i1.18812

Abstract

Strawberry plants are a type of plant that grows extensively in Indonesia. Some studies show that Strawberries (Fragaria x ananassa) are a source of bioactive compounds, rich in ascorbic acid, anthocyanins and phenol compounds, and have high levels of antioxidants. Strawberries have compounds that provide pigment and antioxidant activity, namely anthocyanins. This study aims to detect secondary metabolite compounds in strawberry fruit extract (Fragaria x ananassa) using Thin Layer Chromatography (TLC) with a mobile phase containing butanol, acetic acid, and aqua-distillate (4:1:1) and infrared spectrophotometry. The results of phytochemical screening tests showed that the strawberry extract contained secondary metabolite compounds, including alkaloids, flavonoids, tannins, and saponins. TLC with the Rf value in Strawberry fruit extract results in 0.38. The results of the infrared spectrophotometry analysis indicate the presence of functional groups, including C-H, CO, OH, C꓿C, and ꓿C-O-C, which corroborate the results of TLC, suggesting that the strawberry extract contains anthocyanins. Keywords: strawberry, secondary metabolite compound, TLC, infrared spectrophotometry ABSTRAK Tanaman stroberi merupakan tanaman yang banyak tumbuh di Indonesia. Tanaman stroberi memiliki banyak manfaat terutama bagian buahnya. Buah stroberi (Fragaria x ananassa) adalah sumber senyawa bioaktif, kaya akan asam askorbat, antosianin dan senyawa fenol, dan memiliki kadar antioksidan yang cukup tinggi. Buah stroberi memiliki senyawa yang memberi pigmen dan mempunyai aktivitas antioksidan yaitu antosianin. Penelitian ini bertujuan untuk mengetahui profil senyawa metabolit sekunder pada ekstrak buah stroberi dan mendeteksi senyawa antosianin pada ekstrak buah stroberi (Fragaria x ananassa) menggunakan Kromatografi Lapis Tipis (KLT) dengan fase gerak butanol-asam asetat-aquadest (4:1:1) dan FTIR. Hasil uji skrining fitokimia menunjukkan bahwa ekstrak stroberi mengandung senyawa metabolit sekunder alkaloid, flavonoid, tanin dan saponin. Hasil KLT ditunjukkan dengan nilai Rf pada ekstrak buah stroberi adalah 0,38. Hasil analisis spektrofotometri inframerah terdapat gugus fungsi O–H, C=O, C=C dan =C–O–C yang memperkuat hasil KLT bahwa ekstrak stroberi mengandung antosianin. Kata kunci: stroberi, antosianin, metabolit sekunder, KLT, FTIR
Co-Authors Achyar, Zhahrotun Khofifah Nur Alifia, Ersa Ari Hayati Asari, Devi Indrayani Fitri Azizi, Rosita Imro’atul Dewi, Lina Purnama Dita Agisimanto Dita Agisimanto Emeliya, Emeliya Fitri, Hanin Rahma Hadi, Muhammad Sholikhul Haikal, Muhammad Wildan Istifadah, Hayati Istiqomah, Nuri Lailatul Marista, Bilqis Mawardi, Azis Muhammad Ni'amul Albab Mustakim, Moch Muzayyan, Abdullah Rozzen Nindi Afifa Nisa Nisa, Kholisatun Nurul Jadid Mubarokati Oey, Ulfi Abdul Rahman Paramitha, Armanda Bella Prasetyowati, Eka Purnamasari, Viska Puspa, Andria Putri, Nela Vede Rhofa Rafida Azizah Rafsanjani, Ramizard Ramadhan, Majida Reza Priski Dwi Jayanti Salsabilla, Dyah Ajeng Saraswati Subagjo, Saraswati Setyowati, Dewi Anjar Syafitri, Fitria Indah Tintrim Rahayu, Tintrim Ulfi Abdul Rahman Oey Vikriansyah, Muchammad Faruq