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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences METAMORFOSA Journal of Biological Sciences Jurnal Ilmu Dasar BERKALA SAINSTEK Jurnal Biologi dan Pembelajarannya (JBP) Biology, Medicine, & Natural Product Chemistry Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Jurnal Sains Alami (Known Nature) Berkala Penelitian Hayati Jurnal Sains Alami (Known Nature) Exhibition and Seminar on Science and Creative Technology – Al Azhar Proceeding
Gatra Ervi Jayanti
Biology Department, Faculty Of Mathematics And Natural Sciences, Universitas Islam Malang, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Education Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health

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Korelasi Warna Ujung Akar dengan Warna Bunga Berdasarkan Anatomi Ujung Akar Anggrek Phalaenopsis, Dendrobium dan Vanda Alifia, Ersa; Rahayu, Tintrim; Jayanti, Gatra Ervi
Buletin Anatomi dan Fisiologi Volume 9, Nomor 1, Tahun 2024
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.9.1.2024.10-19

Abstract

Anggrek merupakan tanaman hias yang memiliki ciri khas bunga yang indah dan bentuk bunga yang membedakan dengan tanaman lainnya. Keragaman anggrek yang cukup tinggi di Indonesia, dibutuhkan penelitian untuk menunjukkan korelasi antara warna ujung akar dengan warna bunga anggrek, yang dapat dilihat dari anatomi ujung akar anggrek. Tujuan penelitian ini yaitu untuk mengetahui korelasi warna pada ujung akar dengan warna bunga anggrek  Phalaenopsis, Dendrobium dan Vanda. Penelitian ini menggunakan metode kualititatif secara deskriptif dengan menampilkan data dalam bentuk gambar dan deskripsi dari 9 sampel anggrek dengan warna yang berbeda, dan dianalisis dengan kuantitatif berdasarkan kode warna yang tertera pada Royal Horticultural Society (RHS) dikombinasikan dengan Red, Green, Blue (RGB), uji korelasi warna dilakukan menggunakan Korelasi Pearson. Hasil yang diperoleh pada penelitian ini menunjukkan warna ujung akar dengan warna bunga anggrek Phalaenopsis, Dendrobium dan Vanda terdapat adanya pigmen pembentuk warna klorofil, antosianin dan karotenoid yang dapat dilihat dari anatomi ujung akar pada sel korteks. Vanda memiliki nilai korelasi yang diperoleh sebesar 0,661 dan dinyatakan mempunyai kesamaan hubungan positif dengan kekuatan korelasi paling kuat dibandingkan anggrek Phalaenopsis dan Dendrobium.  Kesimpulan, yaitu warna ujung akar dengan bunga anggrek Phalaenopsis, Dendrobium dan Vanda memiliki korelasi warna yang dapat dilihat dari anatomi ujung akar irisan melintang pada sel korteks.  Orchids are ornamental plants that have beautiful flower characteristics and flower shapes that distinguish them from other plants. The diversity of orchids is quite high in Indonesia, research is needed to show the correlation between the color of the root tip and the color of the orchid flower, which can be seen from the anatomy of the root tip of the orchid. The purpose of this study was to determine the correlation of the color at the tip of the root with the color of the Phalaenopsis, Dendrobium and Vanda orchids. This study used a descriptive qualitative method by displaying data in the form of pictures and descriptions of 9 samples of orchids with different colors, and analyzed quantitatively based on the color codes listed on the Royal Horticultural Society (RHS) combined with Red, Green, Blue (RGB), color correlation test was performed using Pearson Correlation. The results obtained in this study showed that the color of the root tips with the colors of Phalaenopsis, Dendrobium and Vanda orchids contained pigments that form the color of chlorophyll, anthocyanins and carotenoids which can be seen from the anatomy of the root tips in the cortical cells. Vanda has a correlation value of 0.661 and is stated to have the same positive relationship with the strongest correlation strength compared to Phalaenopsis and Dendrobium orchids. The conclusion is that the color of the root tip with Phalaenopsis, Dendrobium and Vanda flowers has a color correlation which can be seen from the anatomy of the root tip of the cross section of the cortex cells.
Analisis In Silico dan Aktivitas Antioksidan Senyawa Nano Kompleks Pada Daun dan Cabang Bidara (Ziziphus mauritiana Lamk ): In Silico Analysis and Antioxidant Activity of Nano Complex Compounds on Leaves and Branches of Bidara (Ziziphus mauritiana Lamk) Nisa, Kholisatun; Rahayu, Tintrim; Jayanti, Gatra Ervi
Jurnal SAINS ALAMI (Known Nature) Vol. 6 No. 1 (2023)
Publisher : FMIPA UNISMA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/j.sa.v6i1.13077

Abstract

Bidara (Ziziphus mauritiana Lamk.) is a source of natural antioxidants that contain antioxidant compounds such as flavonoids, carotenoids, phenolic, and ascorbic acid. Antioxidants in this study were in the form of complex compounds without taking any of the compounds (single compounds). In addition to complex compounds, bidara with nano size can play a maximum role in carrying out its function as an antioxidant in the body. This study aims to determine the composition of active compounds that act as antioxidants in leaves, branches, and combinations of bidara leaves through in silico analysis and to determine the effectiveness of antioxidant tests on nano complex compounds of leaves, branches, and combinations of bidara leaves. In silico analysis of active compounds was carried out online using the Dr. website. Duke's Phytochemical and Ethnobotanical Databases, Passonline, Smiles and Stitch. Experimental research using freeze dry so that bidara becomes a nanometer-sized powder. The samples used were leaves, branches and a combination of leaves and branches tested by the 2,2 Diphenyl 1 picrylhydrazyl (DPPH) method. The results of the in silico analysis showed that there are 4 compounds in the bidara plant that act as antioxidants, namely caumarin and rutin in the leaves, batulinic-acid and ceanothic-acid in the branches. The experimental results showed that the antioxidant activity of the three treatments had differences in antioxidant effectiveness. The color change of DPPH indicates the presence of antioxidants, the color of the leaf sample from dark purple to yellow, the color of the branch sample from dark purple to light purple, and the sample of the combination of leaves from dark purple to yellowish cloudy white. The color change indicates that the combination of leaves and branches has high antioxidant activity, then leaves and finally branches. Microscopic observations showed the formation of crystals in the bidara powder. The crystal structure shows an orderly and organized pattern. The active compounds in bidara that act as antioxidants are caumarin, rutin, batulinic-acid, and ceanothic-acid. The combination of leaves and branches has a high antioxidant activity compared to leaves and branches. Keywords: bidara, In silico, complex compound, antioxidant activity.   ABSTRAK Bidara (Ziziphus mauritiana Lamk.) merupakan sumber antioksidan alami yang memiliki kandungan senyawa antioksidan seperti flavonoid, karotenoid, fenolik, dan asam askorbat. Antioksidan dalam penelitian ini berupa senyawa kompleks tanpa mengambil salah satu senyawanya (senyawa tunggal). Selain senyawa kompleks, bidara dengan ukuran nano dapat berperan maksimal dalam menjalankan fungsinya sebagai antioksidan di dalam tubuh. Tujuan penelitian ini untuk mengetahui komposisi senyawa aktif yang berperan sebagai antioksidan pada daun, cabang, dan kombinasi daun-cabang bidara melalui analisis in silico serta untuk mengetahui efektivitas dari uji antioksidan pada senyawa nano kompleks daun, cabang, serta kombinasi daun-cabang bidara. Analisis senyawa aktif melalui in silico dilakukan secara online dengan menggunakan website Dr. Duke’s Phytochemical and Ethnobotanical Databases, Passonline, Smiles dan Stitch. Penelitian eksperimental dengan menggunakan freeze dry sehingga bidara menjadi serbuk berukunan nanometer. Sampel yang digunakan adalah daun, cabang dan kombinasi daun-cabang dan diuji dengan metode 2,2 Diphenyl 1 picrylhydrazyl (DPPH). Hasil analisis in silico terdapat 4 senyawa dalam tanaman bidara yang memiliki peran sebagai antioksidan yaitu caumarin dan rutin pada daun, batulinic-acid dan ceanothic-acid pada cabang. Hasil penelitian eksperimantal menunjukkan bahwa aktivitas antioksidan dari tiga perlakuan memiliki perbedaan efektivitas antioksidan. Perubahan warna DPPH menunjukkan adanya antioksidan, warna pada sampel daun dari ungu pekat menjadi kuning, warna pada sampel cabang dari ungu pekat menjadi ungu muda, dan sampel kombinasi daun-cabang dari ungu pekat menjadi putih keruh kekuningan. Perubahan warna tersebut menunjukkan bahwa kombinasi daun-cabang memiliki aktivitas antioksidan tinggi, kemudian daun dan terakhir cabang. Pada pengamatan mikroskop menunjukkan terbentuknya kristal dalam serbuk bidara. Struktur kristal menunjukkan pola yang teratur dan terorganisir. Senyawa aktif dalam bidara yang berperan sebagai antioksidan adalah caumarin, rutin, batulinic-acid dan ceanothic-acid. Kombinasi daun-cabang mempunyai aktivitas antioksidan yang tinggi dibandingkan daun dan cabang. Kata kunci: bidara, In silico, senyawa kompleks, aktivitas antioksidan.
Pengaruh Suhu terhadap Aktivitas Antioksidan dalam Daun Zaitun (Olea europaea L.) dengan Metode DPPH Oey, Ulfi Abdul Rahman; Rahayu, Tintrim; Jayanti, Gatra Ervi
Jurnal SAINS ALAMI (Known Nature) Vol. 5 No. 1 (2022)
Publisher : FMIPA UNISMA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/j.sa.v5i1.15927

Abstract

Causes of degenerative diseases are caused by activities and unhealthy lifestyle. unhealthy lifestyle which in turn triggers free radicals. free radicals are moleculs that are not oxidized which cause the formation of new molecules that can damage body cells. free radicals can be overcome by giving natural antioxidants, one of which is form the olive plant (Olea europaea L.).The purpose of this study was to determine the effect of temperature treatment on antioxidant activity in olive leaves based on leaf age by calculating the IC50 value. The method used in this research is the DPPH method. Based on the results of the study, it can be concluded that the temperature of 31.6°c in young leaves according to calculations is categorized as a strong antioxidant because it provides a calculated value of 82.778 ppm and 165.093 ppm on old leaves and classified as moderate antioxidants, while temperature of 44°c give dominant level under 31.6°c with IC50 values 123.78 ppm for young leaves and 165.170 ppm for old leaves and classified as moderate antioxidants according to the theory that compounds that have very strong antioxidant activity values IC50 values are less than 50 ppm, strong if the IC50 are between 50 and 100, moderate antioxidant if the IC50 values are feasible 100-250 ppm, weak if the IC50 value is 250-500 ppm and inactive if the IC50 value is more than 500 ppm. and vice versa related to the selection of leaf age also affects the level of antioxidant activity seen from the comparison of results from the two treatments that young leaves have a higher level of antioxidant activity than old leaves in counteracting free radicals.Keywords : Temperature, Antioxidant, Olive (Olea europaea L.), DPPH.ABSTRAKPenyebab penyakit degeneratif disebabkan karena aktivitas dan pola hidup yang kurang sehat. Pola hidup kurang sehat yang pada akhirnya memicu radikal bebas. Radikal bebas adalah molekul yang tidak teroksidasi yang menyebabkan terbentuk molekul baru yang dapat merusak sel tubuh. Radikal bebas dapat diatasi dengan pemberian antioksidan salah satunya dari tumbuhan zaitun (Olea europaea L.). Tujuan dari penelitian ini adalah untuk mengetahui pengaruh perlakuan suhu terhadap aktivitas antioksidan pada daun zaitun berdasarkan umur daun melalui perhitungan nilai IC50. Metode yang digunakan dalam penelitian ini adalah metode DPPH. Berdasarkan hasil penelitian dapat disimpulkan bahwa perlakuan suhu 31.6°C pada daun muda  menurut perhitungan IC50 dikategorikan sebagai antioksidan kuat karena memberikan nilai hasil perhitungan IC50  sebesar 82.778 ppm dan 165.093 ppm pada daun tua dan tergolong antioksidan sedang, sementara pada perlakuan suhu 44°C memberikan pengaruh lebih rendah dibandingkan dengan perlakuan suhu 31.6°C dengan nilai IC50 sebesar 123.78 ppm untuk daun muda dan 165.170 ppm untuk daun tua dan tergolong antioksidan sedang sesuai teori bahwa senyawa yang memiliki nilai aktivitas antioksidan sangat kuat jika nilai IC50 kurang dari 50 ppm, kuat apabila nilai IC50 bernilai antara 50 sampai 100, antioksidan sedang apabila jika nilai IC50 bernilai 100-250 ppm, lemah jika nilai IC50 bernilai 250-500 ppm dan tidak aktif jika nilai IC50 bernilai lebih dari 500 ppm, begitupun sebaliknya terkait pemilihan umur daun juga berpengaruh terhadap tinggi rendahnya aktivitas antioksidan dilihat dari perbandingan hasil dari kedua perlakuan bahwa daun muda memiliki tingkat aktivitas antioksidan lebih tinggi dibandingkan dengan daun tua dalam menangkal radikal bebas.Kata Kunci : Suhu, Antioksidan, Zaitun (Olea europaea L.), DPPH.
Pengaruh variasi konsentrasi indole butyric acid (iba) pada anggrek (dendrobium hybrid) terhadap pertumbuhan dan survival dalam media cocopeat: Effect of indole butyric acid (iba) concentration variations on orchid (dendrobium hybrid) on growth and survival in cocopeat media Setyowati, Dewi Anjar; Rahayu, Tintrim; Jayanti, Gatra Ervi; Agisimanto, Dita
Jurnal SAINS ALAMI (Known Nature) Vol. 5 No. 2 (2023)
Publisher : FMIPA UNISMA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/j.sa.v5i2.16959

Abstract

Orchids have endemic flowers with high aesthetic value and are widespread throughout Indonesia. One of the widely cultivated orchid varieties is the Dendrobium Orchid. The orchid cultivation process requires special handling, especially in the growing environment, which must be by the plant’s original habitat, so that plant growth and development become more optimal, especially the planting medium and the provision of nutrients must be appropriate. This study aimed to determine the optimal concentration of IBA on the growth of orchid plants (Dendrobium hybrid) and to determine the optimal variation of IBA concentration on the survival of orchid plants in cocopeat media. The research method was carried out experimentally using the Randomized Block Design (RAK). To compare the growth between the concentration of IBA and root growth has given four treatments: P1: 0.25 ml/L, P2: 0.50 ml/L, P3: 0, 75ml/L, P4: 1ml/L each treatment was given three replications and each replication contained four plant seeds with a total of 48 plant seeds. Observation parameters included root length, number of new roots, leaf length, number of leaves, plant length, plant wet weight, and plant survival. The optimal concentration of IBA on orchid growth at a concentration of 0.25 ml/L IBA optimal orchid growth was found in the parameters of the number of new roots. Plant length at a concentration of 0.75 ml/L IBA optimal orchid growth was found in root length and planted wet weight parameters. In the survival parameters of orchid plant life, the optimal concentration of IBA was found at a concentration of 0.25 ml/L.  Keywords: Orchid, growth, orchid survival, and IBA   ABSTRAK Anggrek memiliki bunga endemik yang memiliki nilai estetika tinggi dan menyebar luas di seluruh Indonesia. Salah satu varietas anggrek yang banyak dibudidayakan yaitu Anggrek Dendrobium. Proses budidaya anggrek memerlukan penanganan khusus terutama pada lingkungan tumbuh yang harus sesuai dengan habitat asli tanaman, agar pertumbuhan dan perkembangan tanaman menjadi lebih optimal terutama media tanam dan pemberian unsur hara harus tepat. Tujuan penelitian ini adalah untuk mengetahui konsentrasi pemberian IBA yang optimal terhadap pertumbuhan tanaman angrek (Dendrobium hybrid) serta untuk mengetahui pemberian variasi konsentrasi IBA yang optimal terhadap survival kehidupan tanaman anggrek dalam media cocopeat. Metode penelitian dilakukan secara eksperimental dengan menggunakan Rancangan Acak Kelompok (RAK), untuk membandingkan pertumbuhan antara konsentrasi IBA terhadap pertumbuhan akar yang diberi empat perlakuan P1: 0,25 ml/L, P2: 0,50 ml/L, P3: 0,75ml/L, P4: 1ml/L masing-masing perlakuan di berikan tiga ulangan dan setiap ulangan terdapat empat bibit tanaman dengan jumlah keseluruhan 48 bibit tanaman. Parameter pengamatan meliputi panjang akar, jumlah akar baru, panjang daun, jumlah daun, panjang tanaman, berat basah tanaman, dan survival tanaman. Konsentrasi IBA yang optimal terhadap pertumbuhan anggrek pada konsentrasi 0,25 ml/L IBA, pertumbuhan anggrek yang optimal terdapat pada parameter jumlah akar baru. Pada konsentrasi 0,50 ml/L IBA pertumbuhan anggrek yang optimal terdapat pada parameter panjang daun, jumlah daun, dan panjang tanaman. Pada konsentrasi 0,75 ml/L IBA pertumbuhan anggrek yang optimal terdapat pada parameter panjang akar dan berat basah tanaman. Pada parameter survival kehidupan tanaman anggrek konsentrasi pemberian IBA yang optimal terdapat pada konsentrasi 0,25 ml/L. Kata kunci: Anggrek, pertumbuhan, survival anggrek, dan IBA
Kajian Akar Kadaka sebagai Media Tanam dengan Pengaruh Variasi Konsentrasi Indole Butyric Acid Tterhadap Pertumbuhan Anggrek Dendrobium canaliculatum: Study of Kadaka Roots as a Plant Media with the Effect of Variation Concentrations of Indole Butyric Acid on Dendrobium canaliculatum Orchid Growth Hadi, Muhammad Sholikhul; Rahayu, Tintrim; Jayanti, Gatra Ervi; Agisimanto, Dita
Jurnal SAINS ALAMI (Known Nature) Vol. 6 No. 1 (2023)
Publisher : FMIPA UNISMA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/j.sa.v6i1.17140

Abstract

Dendrobium is an orchid that many orchid lovers like. Efforts to increase the quantity of orchids in the growth phase can be done in several ways, namely through the regulation of fertilization and the use of appropriate planting media. Planting media from kadaka roots is the right media for the growth of orchid. The addition of nutrients N, P, and K together showed a significant increase in total biomass. In addition to fertilizers, growth regulators substances also affect growth. The plant hormone used in plant cultivation is the auxin hormone. One type of auxin hormone type is Indole Butyric Acid (IBA) which functions to stimulate root formation. This study aimed to study the effect of IBA concentration on the root growth of Dendrobium orchids grown on kadaka root media. Dendrobium orchids seedlings were selected beforehand, planted in kadaka roots, and treated twice a week. The treatment was carried out for two months using IBA concentrations of 0.25, 0.50, 0.75, and 1.0 ml/L. The administration of IBA hormone on the growth of Dendrobium canaliculatum affects the number of new roots, leaf length, and plant height. IBA at 0.25 ml/L was the optimal concentration for the number of new roots. The number of new roots as an indicator of optimal orchid growth in response to IBA hormone administration.  Keywords: Kadaka, Indole Butyric Acid (IBA), Orchid, Orchid Growth   ABSTRAK Dendrobium adalah anggrek yang banyak disukai pecinta anggrek. Upaya peningkatan kuantitas anggrek pada fase pertumbuhan dapat dilakukan dengan beberapa cara yaitu melalui pengaturan pemupukan serta penggunaan media tanam yang sesuai. Media tanam dari akar kadaka merupakan media yang tepat untuk pertumbuhan anggrek. Penambahan unsur hara N, P, dan K pada media secara bersama menunjukkan peningkatan total biomassa yang signifikan. Selain pupuk, Zat Pengatur Tumbuh (ZPT) juga berpengaruh terhadap pertumbuhan. Hormon tanaman yang dipergunakan pada pembudidayaan tanaman adalah hormon auksin. Salah satu jenis hormon auksin merupakan Indole Butyric Acid (IBA) yang dipergunakan untuk merangsang pembentukan akar. Penelitian ini bertujuan untuk mengkaji efek konsentrasi IBA terhadap pertumbuhan akar anggrek Dendrobium yang ditanam pada media akar kadaka. Tanaman anggrek yang digunakan adalah seedling Dendrodium, dilakukan seleksi, ditanam di akar kadaka, dan diberi perlakuan dua kali seminggu. Perlakuan dilakukan selama dua bulan menggunakan konsentrasi IBA 0,25, 0,50, 0,75, dan 1,0 ml/L. Pemberian hormon IBA terhadap pertumbuhan anggrek Dendrobium canalicullatum berpengaruh pada jumlah akar baru, panjang daun dan tinggi tanaman. IBA pada 0,25 ml/L merupakan konsentrasi yang optimal untuk jumlah akar baru. Jumlah akar baru sebagai indikator pertumbuhan anggrek yang optimal terhadap respon pemberian hormon IBA.  Kata kunci: Kadaka, Indole Butyric Acid (IBA), Anggrek, Pertumbuhan
Pengaruh Ekstrak Kulit Umbi Bawang Merah (Allium cepa) dan Bawang Putih (Allium sativum) untuk Pertumbuhan Anggrek Dendrobium: The Effect of Shallot (Allium cepa) and Garlic (Allium sativum) Skin Extracts on Dendrobium Orchid Growth Marista, Bilqis; Rahayu, Tintrim; Jayanti, Gatra Ervi
Jurnal SAINS ALAMI (Known Nature) Vol. 6 No. 2 (2024)
Publisher : FMIPA UNISMA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/j.sa.v6i2.18809

Abstract

Genetic and physiological factors influence dendrobium orchids growth and flowering. In maintaining Dendrobium orchids, the cultivation process after acclimatization greatly affects growth and development, so its quality and sustainability can be threatened. Onion bulb skin and garlic bulb skin were able to increase germination and growth variables. This research aims to determine the effect of the most effective concentration on the growth of Dendrobium orchids. This research method used an experimental method with a completely randomized design (CRD) with 24 plants, research parameters included the number of new shoots, number of new shoots, number of new roots, new root length, leaf length, total number of leaves, plant height, and weight. Plants were analyzed descriptively, and multivariate correlation tests were using IBM software. The results showed that the M40: P20 treatment was the best treatment with parameters that responded, namely the number of new shoots, length of new roots, length of leaves, total number of leaves and plant height. This shows that the administration of shallot bulb skin extract and garlic bulb skin extract positively affected several parameters. Keywords : acclimatization, Dendrobium, effectiveness, parameters, plants.   ABSTRAK Anggrek Dendrobium dalam pertumbuhan dan pembungaan dipengaruhi faktor genetik dan faktor fisiologis. Dalam pemeliharaan anggrek Dendrobium proses budidaya setelah aklimatisasi sangat mempengaruhi pertumbuhan dan perkembangan, sehingga kualitas dan kelestraiannya dapat terancam. Kulit umbi bawang merah dan kulit umbi bawang putih mampu meningkatkan perkecambahan dan variabel pertumbuhan. Tujuan penelitian ini yaitu untuk mengetahui pengaruh dari konsentrasi yang paling efektif terhadap pertumbuhan anggrek Dendrobium. Metode penelitian menggunakan eksperimental Rancangan Acak Lengkap (RAL) dengan 24 tanaman, parameter penelitian meliputi jumlah tunas baru, jumlah daun tunas baru, jumlah akar baru, panjang akar baru, panjang daun, total jumlah daun, tinggi tanaman, dan berat kering tanaman yang dianalisis secara deskriptif dan uji korelasi multivariat test menggunakan software IBM. Hasil penelitian menunjukkan perlakuan M40: P20 adalah perlakuan terbaik dengan parameter yang merespon yaitu jumlah daun tunas baru, panjang akar baru, panjang daun, jumlah total daun dan tinggi tanaman. Hal ini menunjukkan bahwa pemberian ekstrak kulit umbi bawang merah dan kulit umbi bawang putih memberikan pengaruh positif pada beberapa perameter. Kata kunci: aklimatisasi, Dendrobium, efektif, parameter, tanaman.
The Effect of the Type of Planting Media and The Addition of O2 Nanobubbles on the Growth of Dendrobium burana Green × Ong Ang Ai Boon Orchid Plantlets in Vitro Purnamasari, Viska; Rahayu, Tintrim; Jayanti, Gatra Ervi; Agisimanto, Dita
Jurnal ILMU DASAR Vol 25 No 1 (2024)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v25i1.41376

Abstract

Dendrobium orchids come in a variety of colors, shapes, and beauty that are in great demand. The process of growing orchids requires special handling of an appropriate growing environment, such as proper planting media and nutrients that can stimulate orchid growth. This type of thin liquid film media is the use of liquid media with an immersion system while vermiculite and perlite media have almost the same properties. However, vermiculite has a higher water absorption capacity than perlite. Nanobubbles are bubbles with 1-100 nm that can live more stably in water. This study aims to influence the type of media and the addition of Nanobubbles O2 on the growth of Dendrobium burana Green × Ong Ang Ai Boon orchid plantlets. This research was carried out from November to December 2022 at the PT. Java Indo Arjuna Singosari, Malang. This study used a completely randomized design (CRD) with 10 treatments, namely solid MS, gaviota solid, thin liquid film, thin liquid film + NBs O2, vermiculite, vermiculite + NBs O2, perlite, perlite + NBs O2, vermiculite + Perlite, vermiculite + perlite + NBs O2 with 4 replications. The data were analyzed descriptively and the Manova test. The results showed that the vermiculite + NBs O2 treatment had the potential to increase the length of plantlets by 13.80 mm, the wet weight of plantlets by 0.30 g, the percentage of live plantlets by 100% whereas in the vermiculite + perlite treatment has the potential for the first root growth to appear, namely 5 HST. The vermiculite treatment had the potential to increase the highest number of leaves by 5 leaves, whereas in the leaf color treatment the solid MS treatment had a more intense green color.
Potential of Ecoenzymes and N2 Nanobubbles on the Growth of Phalaenopsis sp. Orchid at the Acclimatization Stage Syafitri, Fitria Indah; Rahayu, Tintrim; Jayanti, Gatra Ervi
Jurnal ILMU DASAR Vol 25 No 1 (2024)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v25i1.40575

Abstract

Growth is a quantitative change during one plant’s life cycle and is irreversible.withaddition An in crease the volume or dry weight of plants or other organs is due to the adding, of new structural elements. Phalaenopsis (moth orchid) is a genus of orchids appeal. The diversity of colors, shapes, and textures, as well as its aroma, make Phalaenopsis orchids one of the charming flowers of Indonesia. Ecoenzyme is a solution of complex organic substances produced from the fermentation of organic residues, sugar, and water. Nanobubbles (NBs) are an example of nanotechnology. NBs have a diameter of 1-100 nm and are nanoscopic gas bubbles in a solution or water that can change the characteristics of water and are stable. This study aims to determine the potential of giving Ecoenzyme and NBs N2 on the growth of Phalaenopsis orchids during the acclimatization stage. This research was conducted from October to December 2022 at the Orchidology and Nursery Laboratory, Islamic University of Malang. This study used an experimental method with a completely randomized design (CRD) with 10 treatments, namely 0 mL/L (control), 1 mL/L Ecoenzyme, 2 mL/L Ecoenzyme, 3 mL/L Ecoenzyme, 4 mL/L Ecoenzyme, 4 combination NBs N2 and Ecoenzymes, and 5 mL NBs N2 with 3 replications, making a total of 30 treatment units. The research parameters included the number of leaves, leaf length, number of roots, root length, plant height, fresh weight, and plant dry weight. The results of this study showed that the treatment of 3 mL/L Ecoenzyme showed potential in increasing the number of leaves by 4 pieces: at the same time the optimal concentration of the combination of Ecoenzyme and NBs was 2 mL/L plus 5 mL NBs N2 in increasing the number of roots by 10 pieces, and the optimal concentration of NBs was 5 mL in increasing the root length by 7.07 cm, plant height by 17.10 cm leaf. length, plant weight by 4.16 g and dry weight by 0.85 g in Phalaenopsis orchids.
Analysis of Nanobubbles (NBs) Technology and Foliar Fertilization on the Growth of Phalaenopsis sp. Orchid Puspa, Andria; Rahayu, Tintrim; Jayanti, Gatra Ervi
BERKALA SAINSTEK Vol 12 No 2 (2024)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v12i2.39809

Abstract

Plants need nutrients which are then provided through fertilizers, but this can cause long-term problems due to limited absorption by plants so recently implemented NBs technology that can help reduce environmental pollution. This study aimed to determine the effect of NBsO2, NBsH2, NBsCO2, NBsN2 and, NBsO3 on the growth of Phalaenopsis sp. orchid plants. The research method was a complete randomized design (RAL) with 7 treatments (control without application of fertilizer and NBs, foliar fertilizer, NBsO2, NBsH2, NBsCO2, NBsN2, NBsO3) and 4 repeats. NBs were made using a nanogenerator with a flow of 5 liters/ minute and induced for 15 minutes. The data analysis used is the Multivariate Analysis of Variance (MANNOVA) test. The control and NBs treatments were given by dropping 5 ml on each orchid plant. The results showed that leaf length, number of leaves, plant weight and number of plant roots NBsN2 treatment had the best effect, while on root length, NBs O2 treatment had a better effect than other treatments. The application of foliar fertilizer is less effective to meet plant growth needs. At the same time, NBs are more effective because they are very small making it easier for plants to absorb.
The New Technology for In Vitro Culture with Induction of Nanobubbles (NBsN2 and NBsO2) in Cattleya sp. Mustakim, Moch; Mawardi, Azis; Dewi, Lina Purnama; Putri, Nela Vede Rhofa; Rahayu, Tintrim; Jayanti, Gatra Ervi
Biology, Medicine, & Natural Product Chemistry Vol 13, No 2 (2024)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2024.132.459-465

Abstract

The growth and development of orchids take a relatively long time, while the demand for high-quality orchids continues to increase significantly. Tissue culture is a technique of isolating plant parts in organs, tissues, and cells and then culturing these plants on artificial media in a sterile environment. Tissue culture techniques can reproduce plants in a relatively short time, with the same properties and quality, so efforts to optimize tissue culture with technology are the right solution, one of which is using nanogenerator technology that produces Nanobubbles (NBs). NBs are one of the nanotechnologies that are ?100 nm in size with induced gas needed by plants. This study aims to determine the effectiveness of growth and development from the induction of NBsN2 and NBsO2 in Cattleya sp. planlet in vitro culture. This research method uses in vitro culture with a Randomized Group Design. The results of the MsO treatment showed higher weight than other treatments, with a total plant weight value of 0.3550 g and a total plant height value of 1.3983 cm. Murashige-Skoog + NBsNitrogen (MsN) treatment showed higher root length than other treatments with a total value of 1.2367 cm. In the treatment of Ms, MsO, OJ and NJ, a live percentage of 83-100% was observed. The statistical test results indicate that NBs positively affect Cattleya sp. plantlets. This effect includes increasing plant weight, height, and root length and promoting new shoots growth. Additionally, nitrogen supplements play a role in colour changes, indicating the plant's health and photosynthetic efficiency. In the treatment with Induction O2, the initial colour changes from Strong Yellow Green (141 D) to Strong Yellowish Green (141 C), while in the treatment with Induction NBsN2, the initial colour changes from Strong Yellow Green (141 D) to Deep Yellow Green (141 B).
Co-Authors Alifia, Ersa Ari Hayati Asari, Devi Indrayani Fitri Dewi, Lina Purnama Dita Agisimanto Dita Agisimanto Emeliya, Emeliya Fitri, Hanin Rahma Hadi, Muhammad Sholikhul Haikal, Muhammad Wildan Istifadah, Hayati Istiqomah, Nuri Lailatul Jamilah, Zahrotul Ma'rifah, Shofia Jannatul Marista, Bilqis Mawardi, Azis Muhammad Ni'amul Albab Mustakim, Moch Muzayyan, Abdullah Rozzen Nindi Afifa Nisa Nisa, Kholisatun Nuari, Afidah Fajar Nur Azizah Nurul Jadid Mubarokati Oey, Ulfi Abdul Rahman Prasetyowati, Eka Purnamasari, Viska Puspa, Andria Putri, Nela Vede Rhofa Rafida Azizah Rafsanjani, Ramizard Ramadhan, Majida Reza Priski Dwi Jayanti Safitri, Dewi Astriana Salsabilla, Dyah Ajeng Saraswati Subagjo, Saraswati Setyowati, Dewi Anjar Syafitri, Fitria Indah Tintrim Rahayu, Tintrim Ulfi Abdul Rahman Oey Vikriansyah, Muchammad Faruq