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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Ilmu Dasar BERKALA SAINSTEK Biology, Medicine, & Natural Product Chemistry Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Pendas : Jurnah Ilmiah Pendidikan Dasar Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Jurnal Sains Alami (Known Nature) Berkala Penelitian Hayati Biopedagogia Jurnal Sains Alami (Known Nature) Exhibition and Seminar on Science and Creative Technology – Al Azhar Proceeding
Gatra Ervi Jayanti
Biology Department, Faculty Of Mathematics And Natural Sciences, Universitas Islam Malang, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Education Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Other

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Pengaruh Variasi Konsentrasi Indole Butyruc Acid (IBA) Terhadap Pertumbuhan Anggrek (Dendrobium canaliculatum) Pada Media Tumbuh Moss Putih Fitri, Hanin Rahma; Rahayu, Tintrim; Jayanti, Gatra Ervi; Agisimanto, Dita
Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Vol 9 No 2 (2024): January 2024
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/e-jbst.v9i2.507

Abstract

Orchids are ornamental plants that are in demand by the public because of the beauty of their flowers and are very profitable prospects. However, orchids have a long growth phase, with one of the critical phases being acclimatization. The development of the acclimatization phase is a prerequisite for obtaining a high plantlet survival rate. Good plantlet survival is obtained with a good root system using a growth regulator auxin Indole Butyric Acid with white moss growing media. This study aimed to study the effect of variations in IBA concentration on faster root growth of Dendrobium canaliculatum orchids using white moss media. Plantlet Dendrodium canaliculatum seed culture from sub culture 3 and selected first, planted in white moss media, and treated twice a week for a month using 0.25, 0.50, 0.75, and 1.0 ml/L IBA and fertilizer NPK Mamigro 21-21-21 as much as 2 g/L. IBA concentration at 0.25 ml/L was the best concentration for the induction of new roots of Dendrobium canaliculatum orchids grown on white moss and had a high survival rate of 92%.
Respon Pertumbuhan Anggrek Dendrobium sp. terhadap Pemberian Ekoenzim dan Nanobubbles (NBs) CO2 Istifadah, Hayati; Rahayu, Tintrim; Jayanti, Gatra Ervi
Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Vol 10 No 1 (2024): Agustus 2024
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/ejbst.v10i1.547

Abstract

Genus Dendrobium merupakan salah satu anggrek yang banyak diminati karena mampu beradaptasi dengan berbagai kondisi lingkungan tumbuh. Faktor penting yang mempengaruhi pertumbuhan anggrek adalah pemupukan. Pemupukan dengan pupuk organik ekoenzim dan Nanobubbles (NBs) merupakan inovasi untuk meningkatkan pertumbuhan anggrek. Ekoenzim merupakan hasil fermentasi sampah dapur. Sedangkan NBs merupakan salah satu contoh teknologi nano yang memiliki diameter 1-100 nm. NBs telah banyak dimanfaatkan dalam berbagai bidang, berpeluang untuk pertumbuhan anggrek Dendrobium sp. Penelitian ini menggunakan metode eksperimental rancanan acak kelompok (RAK) dengan 10 perlakuan yaitu 0 mL/L (kontrol), 1 mL/L Ekoenzim, 2 mL/L Ekoenzim, 3 mL/L Ekoenzim, 4 mL/L Ekoenzim, 5 ml NBsCO2 dan 4 kombinasi NBsCO2 dan Ekoenzim. Parameter pengamatan meliputi jumlah daun, panjang daun, jumlah akar, panjang akar, tinggi tanaman, berat basah dan berat kering tanaman. Hasil penelitian ini menunjukkanpemberian Ekoenzim 1 mL/L memberikan respon terhadap pertumbuhan jumlah daun (9,67), panjang daun (8,30 cm), tinggi tanaman (9,63 cm), berat kering (0,40 g) dan berat basah (1,05 g) tanaman. Pemberian Ekoenzim 2 mL/L memberikan respon terhadap panjang akar (6,97 cm), sedangkan pemberian 1 mL/L Ekoenzim + 5 ml NBsCO2 memberikan respon terhadap pertumbuhan jumlah akar (14,67). Kata kunci: Pertumbuhan, Dendrobium, Ekoenzim, NBsCO2
Detection of Secondary Metabolites in Cucumber (Cucumis sativus) Leaves and Its Potential as Candidates for Acne Drug Using Histochemical Analysis and In Silico Study Asari, Devi Indrayani Fitri; Rafsanjani, Ramizard; Vikriansyah, Muchammad Faruq; Muzayyan, Abdullah Rozzen; Mubarakati, Nurul Jadid; Jayanti, Gatra Ervi
EXSACT-A Vol 1, No 1 (2023)
Publisher : Universitas Al Azhar Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36722/exc.v1i1.2232

Abstract

Acne is a facial skin problem that is generally experienced by 75-80% of adolescents as a result of excessive production of oil glands and sweat, inflammatory mediators. The acnecausing bacteria, Propionibacterium acnes, involves KAS III and JNK 1 proteins, so it is expected to be an effective anti-inflammatory and antibacterial strategy. Cucumber leaves are known to have biological activity as a candidate for acne medication. This study aims to detect secondary metabolites in cucumber leaves through histochemical and in silico analysis. Histochemical analysis was carried out by preparing fresh leaves through lower leaf incisions by dropping secondary metabolite detection reagents and then observing the color changes microscopically. The in silico test aims to determine the physicochemical properties, pharmacokinetic properties and interactions of the active compounds with KAS III and JNK1 as acne drug targets through molecular docking. Histochemical analysis showed that cucumber leaves positively contained secondary metabolites, namely terpenoids, flavonoids, alkaloids, tannins, and phenols. Meanwhile, it was known that isovitexin, cucurbitacin B & C had fairly good physicochemical properties, but only isovitexin and cucurbitacin C had the best pharmacokinetic properties. Based on the results of molecular docking, there are the same amino acid bonds between isovitexin and the control at the JNK1 receptor, namely the amino acids Ile:32, Val:40 and Leu:168 and the binding affinity value is low so it is predicted to be the most effective in inhibiting JNK1. Therefore, cucumber leaves open up a new potential as a candidate for natural acne preparations.Keywords: acne, cucumber, secondary metabolites, histochemical, in silico
Pengaruh Pemberian Zat Pengatur Tumbuh Hormon Auksin dan Sitokinin Terhadap Pertumbuhan Tunas dan Akar Zaitun (Olea europaea) Menggunakan Metode Microcutting Paramitha, Armanda Bella; Achyar, Zhahrotun Khofifah Nur; Azizi, Rosita Imro’atul; Jayanti, Gatra Ervi; Ramadhan, Majida; Prasetyowati, Eka
Biopedagogia Vol 7, No 2 (2025): November
Publisher : Program Studi Pendidikan Biologi Universitas Borneo Tarakan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35334/biopedagogia.v7i2.6886

Abstract

Olive plants (Olea europaea) are widely used as traditional medicine and have high economic value, but olive cultivation in Indonesia still faces many challenges. This study aims to evaluate the effect of providing plant growth regulators (ZPT) auxin and cytokinin hormones on the growth of olive shoots and roots using the microcutting method. The study was conducted at the Orchidology Laboratory, Islamic University of Malang with an experimental method of three treatments, namely water control, Biogen, and Bregadium. The parameters observed included the number and quality of shoot and root growth. The results showed that Biogen and Bregadium were able to accelerate the emergence of shoots on the 3rd day, while the control grew on the 10th day. Observations of root growth showed that Bregadium was able to stimulate root formation in more samples even though the quality was still very thin and few. In contrast, Biogen only produced one thin root in one sample. The conclusion of the study states that the combination of Bregadium ZPT hormones has the potential to be more effective in stimulating the growth of olive roots and shoots vegetatively. This study recommends optimizing the concentration and frequency of ZPT application for more optimal growth results.
EFEKTIVITAS KOMBINASI EKSTRAK DELIMA PUTIH (Punica granatum) DAN SIRIH MERAH (Piper crocatum) TERHADAP DAYA HAMBAT Staphylococcus aureus SECARA IN VITRO Dea Revi Mahreza; Faisal; Gatra Ervi Jayanti
Pendas : Jurnal Ilmiah Pendidikan Dasar Vol. 11 No. 03 (2026): Volume 11 No. 03, September 2026 Release
Publisher : Program Studi Pendidikan Guru Sekolah Dasar FKIP Universitas Pasundan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23969/jp.v11i03.57813

Abstract

Antibiotic resistance represents a global health crisis that has driven the exploration of natural products as alternative antibacterial agents. This research aimed to evaluate the efficacy of combining white pomegranate peel (Punica granatum) and red betel leaf (Piper crocatum) extracts in inhibiting the growth of Staphylococcus aureus in vitro. A completely randomized design (CRD) was employed using the disc diffusion method. The treatments consisted of combination ratios of 1:1, 1:2, and 2:1 at concentrations of 25, 50, 75, and 100%. Chloramphenicol served as the positive control, while distilled water was used as the negative control. The findings revealed that the 1:2 ratio combination produced the highest inhibition zone (25.50 mm), which was significantly different (p < 0.05) from the positive control. This combination demonstrated a mutually reinforcing effect. It was concluded that the combination of white pomegranate peel extract and red betel leaf extract holds promise as a natural antibacterial agent.
UJI AKTIVITAS ANTIBAKTERI KOMBINASI EKSTRAK SIRIH MERAH (Piper crocatum) DAN DELIMA PUTIH (Punica granatum) TERHADAP Salmonella typhi Sindy Puspitasari; Faisal; Gatra Ervi Jayanti
Pendas : Jurnal Ilmiah Pendidikan Dasar Vol. 11 No. 03 (2026): Volume 11 Nomor 03, September 2026 Published
Publisher : Program Studi Pendidikan Guru Sekolah Dasar FKIP Universitas Pasundan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23969/jp.v11i03.60470

Abstract

Salmonella typhi is a Gram-negative bacterium that causes typhoid fever and remains a significant public health concern, particularly due to the increasing resistance to conventional antibiotics. Natural products such as red betel leaf (Piper crocatum) and white pomegranate peel (Punica granatum), which contain flavonoids, tannins, and polyphenols, have potential as alternative antibacterial agents. This study aimed to determine the antibacterial activity of single and combined extracts of red betel leaf and white pomegranate peel against Salmonella typhi. This experimental study employed the Kirby–Bauer disk diffusion method using a completely randomized design. Chloramphenicol 0.005% was used as the positive control, while sterile distilled water served as the negative control. The treatments consisted of single extracts of red betel leaf, single extracts of white pomegranate peel, and combinations of white pomegranate peel and red betel leaf extracts (P:B) at ratios of 1:1, 1:2, and 2:1 with concentrations of 25%, 50%, 75%, and 100%. The observed parameter was the diameter of the inhibition zone after incubation at 37°C for 24 hours. Data were analyzed using the Shapiro–Wilk test, Levene’s test, One-Way ANOVA, and the Least Significant Difference (LSD) post hoc test. The results showed that red betel leaf extract exhibited moderate antibacterial activity, whereas white pomegranate peel extract demonstrated strong to very strong antibacterial activity. Statistical analysis revealed significant differences among treatments (p≤ 0.05), indicating that the combined extracts produced greater inhibitory effects than the single extracts. The largest inhibition zone diameter was observed in the combination ratio of 1:2 at a concentration of 100%, measuring 25.21 mm, which was categorized as very strong antibacterial activity.
KOMBINASI EKSTRAK DAUN ALPUKAT (Persea americana) DAN DAUN PEPAYA (Carica papaya) TERHADAP DAYA HAMBAT Salmonella typhi SECARA IN VITRO Anggita Berliana Safira; Faisal; Gatra Ervi Jayanti
Pendas : Jurnal Ilmiah Pendidikan Dasar Vol. 11 No. 03 (2026): Volume 11 Nomor 03, September 2026 Published
Publisher : Program Studi Pendidikan Guru Sekolah Dasar FKIP Universitas Pasundan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23969/jp.v11i03.61174

Abstract

Typhoid fever is an infectious disease caused by Salmonella typhi bacteria with a high mortality rate in Indonesia. Inappropriate use of antibiotics can lead to bacterial resistance, so safer natural antibacterial alternatives are needed. Avocado leaves (Persea americana) and papaya leaves (Carica papaya) are known to contain secondary metabolite compounds such as flavonoids, alkaloids, saponins, and tannins that have antibacterial potential. This study aims to test the effectiveness of a combination of avocado and papaya leaf extracts on the inhibition of Salmonella typhi in vitro. This study was a laboratory experimental study with a completely randomized design (CRD) using the Kirby-Bauer disc diffusion method. The extract was prepared by maceration using 96% ethanol. The treatments consisted of a positive control of 0.005% chloramphenicol, a negative control of sterile distilled water, single extracts of avocado and papaya leaves at concentrations of 25, 50% 75, and 100%, and a combination of extracts at a ratio of 1:1, 2:1, and 1:2, each treatment was repeated three times. Data were analyzed using the Kruskal–Wallis test followed by the Mann–Whitney test. The results showed that all treatments were able to inhibit the growth of Salmonella typhi with a moderate inhibitory effect. A single papaya leaf extract had a higher inhibitory effect than avocado leaf extract and several extract combinations. The highest inhibitory effect was obtained from papaya leaf extract at a concentration of 50% with an average inhibition zone diameter of 9.49 mm. Antibacterial activity is influenced by bioactive compounds that work to damage the membrane and cell walls of bacteria. Based on the results of the study, it can be concluded that the combination of avocado leaf and papaya leaf extracts showed an additive antibacterial effect, while a single papaya leaf extract was more effective in inhibiting the growth of Salmonella typhi than the combination of the two extracts.
Co-Authors Achyar, Zhahrotun Khofifah Nur Alifia, Ersa Anggita Berliana Safira Ari Hayati Asari, Devi Indrayani Fitri Azizi, Rosita Imro’atul Dea Revi Mahreza Dewi, Lina Purnama Dita Agisimanto Dita Agisimanto Emeliya, Emeliya Faisal Fitri, Hanin Rahma Hadi, Muhammad Sholikhul Haikal, Muhammad Wildan Istifadah, Hayati Istiqomah, Nuri Lailatul Marista, Bilqis Mawardi, Azis Muhammad Ni&#039;amul Albab Mustakim, Moch Muzayyan, Abdullah Rozzen Nindi Afifa Nisa Nisa, Kholisatun Nurul Jadid Mubarokati Oey, Ulfi Abdul Rahman Paramitha, Armanda Bella Prasetyowati, Eka Purnamasari, Viska Puspa, Andria Putri, Nela Vede Rhofa Rafida Azizah Rafsanjani, Ramizard Ramadhan, Majida Reza Priski Dwi Jayanti Salsabilla, Dyah Ajeng Saraswati Subagjo, Saraswati Setyowati, Dewi Anjar Sindy Puspitasari Syafitri, Fitria Indah Tintrim Rahayu, Tintrim Ulfi Abdul Rahman Oey Vikriansyah, Muchammad Faruq