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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences METAMORFOSA Journal of Biological Sciences Jurnal Ilmu Dasar BERKALA SAINSTEK Jurnal Biologi dan Pembelajarannya (JBP) Biology, Medicine, & Natural Product Chemistry Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Jurnal Sains Alami (Known Nature) Berkala Penelitian Hayati Jurnal Sains Alami (Known Nature) Exhibition and Seminar on Science and Creative Technology – Al Azhar Proceeding
Gatra Ervi Jayanti
Biology Department, Faculty Of Mathematics And Natural Sciences, Universitas Islam Malang, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Education Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health

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Pengaruh Spektrum Warna dan Penambahan Nanobubbles (NBs) Nitrogen (N2) terhadap Pertumbuhan Anggrek Dendrobium sp. secara In Vitro: The Influence of Color Spectrum and Addition of Nitrogen (N2) Nanobubbles (NBs) on the In Vitro Growth of Dendrobium sp. Orchids Salsabilla, Dyah Ajeng; Rahayu, Tintrim; Jayanti, Gatra Ervi
Jurnal SAINS ALAMI (Known Nature) Vol. 8 No. 1 (2025)
Publisher : FMIPA UNISMA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/j.sa.v8i1.18811

Abstract

In in vitro culture, light and nitrogen absorption play crucial roles in the growth of Dendrobium sp orchids. Light affects plant absorption; thus, nitrogen must be added in the right amount for optimal growth. Nanobubbles (NBs) technology has been developed to convert nitrogen into smaller particles, facilitating plant absorption. This study aims to investigate the effects of color spectrum and N2 NBs on the growth of Dendrobium sp orchids. The experimental research method involved different treatments of media (MS; MS+ZPT; MS+ NBs N2; MS+ZPT+NBs N2) and color spectrum (white, red, blue, red-blue). Data on live percentage, plantlet legth and plantlet weight were analyzed descriptively and statistically (normality, homogeneity, multivariate, and Games-Howell tests). The results showed that the highest percentage of live plantlets, 97.91%, was found in the treatments with red and red-blue color spectrum. The red-blue color spectrum significantly increased the plantlet weight by 0.363 g in the MS treatment, and the application of red color spectrum potentially increased the plantlet length by 1.707 mm in the MS+ZPT treatment. The addition of NBs N2 in the subculture media showed potential for 100% live plantlet percentage in the MS+NBs N2 treatment. Keywords: Dendrobium, nanobubbles (NBs), color spectrum, in vitro, Nitrogen ABSTRAK Dalam kultur in vitro, penyerapan cahaya dan nitrogen berperan penting terhadap pertumbuhan anggrek Dendrobium sp. Cahaya mempengaruhi penyerapan tanaman, sehingga nitrogen harus ditambahkan dalam jumlah yang tepat untuk pertumbuhan optimal. Teknologi Nanobubbles (NBs) telah dikembangkan untuk mengubah nitrogen menjadi partikel yang lebih kecil agar memudahkan penyerapan oleh tanaman. Penelitian ini bertujuan untuk mengetahui pemberian spektrum warna dan NBs  terhadap pertumbuhan anggrek Dendrobium sp. Metode penelitian eksperimental dengan perlakuan media (MS; MS ₊ ZPT; MS ₊ NBs ; MS ₊ ZPT ₊ NBs ) dan spektrum warna (putih, merah, biru, merah-biru). Data persentase hidup, panjang planlet dan berat planlet dianalisis secara deskriptif dan uji statistik (normalitas, homogenitas, multivariat, dan Games Howell). Hasil penelitian menunjukkan bahwa pada persentase hidup planlet tertinggi yaitu 97,91% terdapat pada perlakuan spektrum warna merah dan merah-biru. Spektrum warna merah-biru berpengaruh dalam meningkatkan berat planlet sebesar 0,363 g pada perlakuan MS dan pemberian spektrum warna merah berpotensi meningkatkan panjang planlet sebesar 1,707 mm pada perlakuan MS + ZPT. Dalam pemberian NBs  dalam media subkultur memberikan potensi persentase hidup planlet sebesar 100% pada perlakuan MS ₊ NBs .  Kata kunci: Dendrobium, Nanobubbles (NBs), Spektrum warna, Nitrogen
Profil Senyawa Metabolit Sekunder dan Deteksi Antosianin pada Buah Stroberi (fragaria x ananassa) Menggunakan Kromatografi Lapis Tipis dan Spektrofotometri Inframerah: Profile of Secondary Metabolite Compounds and Anthocyanin Detection in Strawberry Fruit (Fragaria x ananassa) Using Thin-Layer Chromatography and Infrared Spectrophotometry Haikal, Muhammad Wildan; Rahayu, Tintrim; Jayanti, Gatra Ervi
Jurnal SAINS ALAMI (Known Nature) Vol. 8 No. 1 (2025)
Publisher : FMIPA UNISMA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/j.sa.v8i1.18812

Abstract

Strawberry plants are a type of plant that grows extensively in Indonesia. Some studies show that Strawberries (Fragaria x ananassa) are a source of bioactive compounds, rich in ascorbic acid, anthocyanins and phenol compounds, and have high levels of antioxidants. Strawberries have compounds that provide pigment and antioxidant activity, namely anthocyanins. This study aims to detect secondary metabolite compounds in strawberry fruit extract (Fragaria x ananassa) using Thin Layer Chromatography (TLC) with a mobile phase containing butanol, acetic acid, and aqua-distillate (4:1:1) and infrared spectrophotometry. The results of phytochemical screening tests showed that the strawberry extract contained secondary metabolite compounds, including alkaloids, flavonoids, tannins, and saponins. TLC with the Rf value in Strawberry fruit extract results in 0.38. The results of the infrared spectrophotometry analysis indicate the presence of functional groups, including C-H, CO, OH, C꓿C, and ꓿C-O-C, which corroborate the results of TLC, suggesting that the strawberry extract contains anthocyanins. Keywords: strawberry, secondary metabolite compound, TLC, infrared spectrophotometry ABSTRAK Tanaman stroberi merupakan tanaman yang banyak tumbuh di Indonesia. Tanaman stroberi memiliki banyak manfaat terutama bagian buahnya. Buah stroberi (Fragaria x ananassa) adalah sumber senyawa bioaktif, kaya akan asam askorbat, antosianin dan senyawa fenol, dan memiliki kadar antioksidan yang cukup tinggi. Buah stroberi memiliki senyawa yang memberi pigmen dan mempunyai aktivitas antioksidan yaitu antosianin. Penelitian ini bertujuan untuk mengetahui profil senyawa metabolit sekunder pada ekstrak buah stroberi dan mendeteksi senyawa antosianin pada ekstrak buah stroberi (Fragaria x ananassa) menggunakan Kromatografi Lapis Tipis (KLT) dengan fase gerak butanol-asam asetat-aquadest (4:1:1) dan FTIR. Hasil uji skrining fitokimia menunjukkan bahwa ekstrak stroberi mengandung senyawa metabolit sekunder alkaloid, flavonoid, tanin dan saponin. Hasil KLT ditunjukkan dengan nilai Rf pada ekstrak buah stroberi adalah 0,38. Hasil analisis spektrofotometri inframerah terdapat gugus fungsi O–H, C=O, C=C dan =C–O–C yang memperkuat hasil KLT bahwa ekstrak stroberi mengandung antosianin. Kata kunci: stroberi, antosianin, metabolit sekunder, KLT, FTIR
Pengaruh Variasi Konsentrasi Indole Butyruc Acid (IBA) Terhadap Pertumbuhan Anggrek (Dendrobium canaliculatum) Pada Media Tumbuh Moss Putih Fitri, Hanin Rahma; Rahayu, Tintrim; Jayanti, Gatra Ervi; Agisimanto, Dita
Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Vol 9 No 2 (2024): January 2024
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/e-jbst.v9i2.507

Abstract

Orchids are ornamental plants that are in demand by the public because of the beauty of their flowers and are very profitable prospects. However, orchids have a long growth phase, with one of the critical phases being acclimatization. The development of the acclimatization phase is a prerequisite for obtaining a high plantlet survival rate. Good plantlet survival is obtained with a good root system using a growth regulator auxin Indole Butyric Acid with white moss growing media. This study aimed to study the effect of variations in IBA concentration on faster root growth of Dendrobium canaliculatum orchids using white moss media. Plantlet Dendrodium canaliculatum seed culture from sub culture 3 and selected first, planted in white moss media, and treated twice a week for a month using 0.25, 0.50, 0.75, and 1.0 ml/L IBA and fertilizer NPK Mamigro 21-21-21 as much as 2 g/L. IBA concentration at 0.25 ml/L was the best concentration for the induction of new roots of Dendrobium canaliculatum orchids grown on white moss and had a high survival rate of 92%.
Respon Pertumbuhan Anggrek Dendrobium sp. terhadap Pemberian Ekoenzim dan Nanobubbles (NBs) CO2 Istifadah, Hayati; Rahayu, Tintrim; Jayanti, Gatra Ervi
Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Vol 10 No 1 (2024): Agustus 2024
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/ejbst.v10i1.547

Abstract

Genus Dendrobium merupakan salah satu anggrek yang banyak diminati karena mampu beradaptasi dengan berbagai kondisi lingkungan tumbuh. Faktor penting yang mempengaruhi pertumbuhan anggrek adalah pemupukan. Pemupukan dengan pupuk organik ekoenzim dan Nanobubbles (NBs) merupakan inovasi untuk meningkatkan pertumbuhan anggrek. Ekoenzim merupakan hasil fermentasi sampah dapur. Sedangkan NBs merupakan salah satu contoh teknologi nano yang memiliki diameter 1-100 nm. NBs telah banyak dimanfaatkan dalam berbagai bidang, berpeluang untuk pertumbuhan anggrek Dendrobium sp. Penelitian ini menggunakan metode eksperimental rancanan acak kelompok (RAK) dengan 10 perlakuan yaitu 0 mL/L (kontrol), 1 mL/L Ekoenzim, 2 mL/L Ekoenzim, 3 mL/L Ekoenzim, 4 mL/L Ekoenzim, 5 ml NBsCO2 dan 4 kombinasi NBsCO2 dan Ekoenzim. Parameter pengamatan meliputi jumlah daun, panjang daun, jumlah akar, panjang akar, tinggi tanaman, berat basah dan berat kering tanaman. Hasil penelitian ini menunjukkanpemberian Ekoenzim 1 mL/L memberikan respon terhadap pertumbuhan jumlah daun (9,67), panjang daun (8,30 cm), tinggi tanaman (9,63 cm), berat kering (0,40 g) dan berat basah (1,05 g) tanaman. Pemberian Ekoenzim 2 mL/L memberikan respon terhadap panjang akar (6,97 cm), sedangkan pemberian 1 mL/L Ekoenzim + 5 ml NBsCO2 memberikan respon terhadap pertumbuhan jumlah akar (14,67). Kata kunci: Pertumbuhan, Dendrobium, Ekoenzim, NBsCO2
Detection of Secondary Metabolites in Cucumber (Cucumis sativus) Leaves and Its Potential as Candidates for Acne Drug Using Histochemical Analysis and In Silico Study Asari, Devi Indrayani Fitri; Rafsanjani, Ramizard; Vikriansyah, Muchammad Faruq; Muzayyan, Abdullah Rozzen; Mubarakati, Nurul Jadid; Jayanti, Gatra Ervi
EXSACT-A Vol 1, No 1 (2023)
Publisher : Universitas Al Azhar Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36722/exc.v1i1.2232

Abstract

Acne is a facial skin problem that is generally experienced by 75-80% of adolescents as a result of excessive production of oil glands and sweat, inflammatory mediators. The acnecausing bacteria, Propionibacterium acnes, involves KAS III and JNK 1 proteins, so it is expected to be an effective anti-inflammatory and antibacterial strategy. Cucumber leaves are known to have biological activity as a candidate for acne medication. This study aims to detect secondary metabolites in cucumber leaves through histochemical and in silico analysis. Histochemical analysis was carried out by preparing fresh leaves through lower leaf incisions by dropping secondary metabolite detection reagents and then observing the color changes microscopically. The in silico test aims to determine the physicochemical properties, pharmacokinetic properties and interactions of the active compounds with KAS III and JNK1 as acne drug targets through molecular docking. Histochemical analysis showed that cucumber leaves positively contained secondary metabolites, namely terpenoids, flavonoids, alkaloids, tannins, and phenols. Meanwhile, it was known that isovitexin, cucurbitacin B & C had fairly good physicochemical properties, but only isovitexin and cucurbitacin C had the best pharmacokinetic properties. Based on the results of molecular docking, there are the same amino acid bonds between isovitexin and the control at the JNK1 receptor, namely the amino acids Ile:32, Val:40 and Leu:168 and the binding affinity value is low so it is predicted to be the most effective in inhibiting JNK1. Therefore, cucumber leaves open up a new potential as a candidate for natural acne preparations.Keywords: acne, cucumber, secondary metabolites, histochemical, in silico
In silico and Histochemical Analysis of Soursop Leaves (Annona muricata) Against Alpha Estrogen Receptor Nuari, Afidah Fajar; Jayanti, Gatra Ervi; Ramadhan, Majida
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.1055-1068

Abstract

Soursop leaves (Annona muricata) are known as a plant that has the potential to treat breast cancer, which has a high mortality rate in women in Indonesia. The receptor that plays the most important role in breast cancer development is the Estrogen Receptor Alpha (ERa). This study aims to identify active compounds in soursop leaves that have the potential to inhibit breast cancer cell growth through in silico analysis using molecular docking of the ER? receptor using KNApSAck, PASS Online, PubChem, PDB ID, PyRx, and Discovery Studio software and Histochemical analysis was performed using fresh soursop leaves by making an incision on the lower part of the leaf and adding reagents AlCl, FeCl, Na2CO, CuSO4, glycerin, Wagner reagent, immersion oil, and ethanol, then observed microscopically. The in silico results showed the presence of specific compounds from soursop leaves and one reference compound (OHT600). Cis-Solamin had the highest binding affinity (-9.3 kcal/mol), better than the native ligand (-7.6 kcal/mol), and interacted with the amino acid Glutamine 532 (GLU532). Histochemical analysis showed the content of flavonoids, phenols, tannins, terpenoids, and alkaloids, accompanied by color changes in trichome and stomatal tissues. Based on the research data, it was concluded that soursop leaves can inhibit the growth of breast cancer cells through the content of secondary metabolites that actively inhibit Er? receptors.
Pengaruh Gas Etilen Tomat (Solanum lycopersicum) terhadap Pertumbuhan Tanaman Kacang Hijau (Vigna radiata L.) Nur Azizah; Ma'rifah, Shofia Jannatul; Jamilah, Zahrotul; Safitri, Dewi Astriana; Jayanti, Gatra Ervi; Ramadhan, Majida; Prasetyowati, Eka
Jurnal Biologi dan Pembelajarannya (JB&P) Vol 12 No 2 (2025): OKTOBER 2025
Publisher : Universitas Nusantara PGRI Kediri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29407/jbp.v12i2.25867

Abstract

Kacang hijau (Vigna radiata L.) termasuk ke dalam suku polong-polongan yang mengandung bahan baku berprotein nabati tinggi. Pertumbuhan tanaman kacang hijau dapat dipengaruhi oleh pemberian hormon termasuk hormon etilen yang dapat diperoleh secara alami dari buah. Penelitian ini bertujuan untuk mengetahui pengaruh dari gas etilen alami yang dihasilkan oleh buah tomat (Solanum lycopersicum) terhadap pertumbuhan tanaman kacang hijau (Vigna radiata L.). Gas etilen merupakan hormon tumbuhan berbentuk gas yang berperan dalam proses pematangan dan penuaan, namun pada fase awal pertumbuhan tanaman, gas ini dapat memengaruhi proses perkecambahan dan perkembangan vegetatif. Penelitian dilakukan secara eksperimental selama tujuh hari di Laboratorium Orchidologi Universitas Islam Malang, dengan menggunakan empat perlakuan, yaitu satu kontrol (tanpa tomat) dan tiga perlakuan dengan penambahan tomat. Parameter yang diamati meliputi tinggi batang, bentuk batang, jumlah daun, dan kondisi akar. Hasil penelitian menunjukkan bahwa perlakuan gas etilen dari tomat menghambat pertumbuhan tanaman kacang hijau. Tanaman yang diberi perlakuan menunjukkan batang tinggi lebih pendek, bentuk batang yang tebal dan kerdil, serta jumlah daun lebih sedikit dibandingkan dengan kontrol. Meskipun akar tetap tumbuh lebat, perkembangan tanaman secara keseluruhan terganggu. Kesimpulannya, gas etilen dari tomat memiliki pengaruh negatif terhadap pertumbuhan awal tanaman kacang hijau, khususnya dalam fase perkecambahan dan pertumbuhan batang serta daun    
Co-Authors Alifia, Ersa Ari Hayati Asari, Devi Indrayani Fitri Dewi, Lina Purnama Dita Agisimanto Dita Agisimanto Emeliya, Emeliya Fitri, Hanin Rahma Hadi, Muhammad Sholikhul Haikal, Muhammad Wildan Istifadah, Hayati Istiqomah, Nuri Lailatul Jamilah, Zahrotul Ma'rifah, Shofia Jannatul Marista, Bilqis Mawardi, Azis Muhammad Ni'amul Albab Mustakim, Moch Muzayyan, Abdullah Rozzen Nindi Afifa Nisa Nisa, Kholisatun Nuari, Afidah Fajar Nur Azizah Nurul Jadid Mubarokati Oey, Ulfi Abdul Rahman Prasetyowati, Eka Purnamasari, Viska Puspa, Andria Putri, Nela Vede Rhofa Rafida Azizah Rafsanjani, Ramizard Ramadhan, Majida Reza Priski Dwi Jayanti Safitri, Dewi Astriana Salsabilla, Dyah Ajeng Saraswati Subagjo, Saraswati Setyowati, Dewi Anjar Syafitri, Fitria Indah Tintrim Rahayu, Tintrim Ulfi Abdul Rahman Oey Vikriansyah, Muchammad Faruq