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All Journal Jurnal Kedokteran Brawijaya The Journal of Experimental Life Sciences (JELS) Biotropika ECSOFiM (Economic and Social of Fisheries and Marine Journal) Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Medik Veteriner
Agung Pramana Warih Marhendra, Agung Pramana Warih
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Physics Social Sciences Veterinary

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PROFIL SIKLUS ESTRUS DAN FOLIKULOGENESIS PADA TIKUS MODEL HIPOTIROIDISM HASIL INDUKSI CAPRINE THYROGLOBULIN (CTG) Haq, Noer Muhammad Dliyaul; Aulanni'am, Aulanni'am; Marhendra, Agung Pramana Warih
Jurnal Kedokteran Brawijaya Vol 29, No 1 (2016)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2016.029.01.2

Abstract

Autoimmune thyroiditis (AITD) merupakan penyakit autoimun pada kelenjar tiroid. Tujuan penelitian ini adalah mengetahui profil regulasi hormon tiroid, profil siklus estrus dan folikulogenesis pada tikus model hypothyroidism hasil induksi capra thyroglobulin (cTg). Pembuatan tikus model hipotiroid dilakukan dengan injeksi dengan protein cTg yang sudah diemulsi complete freund's adjuvant (CFA)  dengan dosis 200µg/µL melalui subkutan, selanjutnya dilakukan booster injeksi protein cTg yang sudah diemulsi incomplete freund's adjuvant (IFA) dengan dosis 200µg/µL melalui subkutan sebanyak dua kali setiap 12 hari. Serum tikus diambil untuk mengetahui tikus sudah mengalami kondisi hipotiroiditis. Tikus yang sudah mengalami hipotiroid, diamati siklus estrusnya sebanyak tiga kali siklus estrus dan setiap satu kali pengulangan siklus estrus diambil serum darah untuk diukur kadar hormon tiroksin (T4) dan thyroid stimulating hormone (TSH). Pada akhir tahapan penelitian, tikus dibedah dan dikoleksi organ ovarium untuk pembuatan preparat histologi. Hasil penelitian menunjukkan bahwa induksi cTg pada tikus menyebabkan tiroiditis dan selanjutnya mengakibatkan kondisi hipotiroid yang ditandai dengan penurunan kadar hormon tiroksin dan peningkatan thyroid stimulating hormone (TSH) selama tiga kali siklus estrus. Tikus hipotiroid mengalami periode siklus estrus yang lebih panjang dan penurunan jumlah folikel de graff dibandingkan dengan tikus normal.
Spermatogenesis Duration on Adult Javan Langur (Trachypithecus auratus) Based on Testosteron Hormone and Luteinizing Hormone (LH) Sangen, Olea Rody; Firmawati, Aulia; Marhendra, Agung Pramana Warih; Titisari, Nurina
Jurnal Medik Veteriner Vol. 4 No. 1 (2021): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss1.2021.110-117

Abstract

This study aimed to determine the duration of the process of spermatogenesis in adult Javan langurs (Trachypithecus auratus) by measuring levels of the hormone testosterone and luteinizing hormone (LH), as one of the efforts to increase the success of mating this endemic animals. Two Javan langurs aged 3-5-year-old, named Luki and Moses were taken their feces for 30 days. Feces extraction process used PBS with a ratio of 1: 9, while the measurement of hormone levels used Monkey Luteinizing Hormone ELISA BT Lab brand with Cat.No E0052MK for LH and Cat No E0008MK for testosterone. Hormone consentration data was a graph with fluctuating patterns which are then analyzed descriptively. The results of this study were the concentration of the hormone testosterone is in the range of 4.81 ng / gram of feces to 15.08 ng / gram of feces. While LH level is in the range of 12.59 mIU/gram to 26.24 mIU/gram. In conclusion, we could estimated that the duration of the spermatogenesis cycle every month in langur Luki occurs for 14 days, while Langur Moses for 10 days.
Potential of Olive Oil Extract (Olea europaea) For Affecting Lipid Profile, Lipid Oxidative and Fatty Liver on Hiperlipemic Rats (Rattus norvegicus) Hanifwati, Annisa; Marhendra, Agung Pramana Warih; Aulanni'am, Aulanni'am
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2019.009.02.12

Abstract

Olive oil (Olea europaea) contains 55 to 83% oleic acid which is a single chain unsaturated fatty acid or mono-unsaturated fatty acid (MUFA), and 2% phenolic components in the form of hydroxytyrosol and tyrosol. This study aims to determine the potential of olive oil extract (Olea europaea) in reducing cholesterol and malondialdehyde levels, along with inhibiting fatty liver development in hiperlipemic rats. Sixteen rats were divided into four groups, the first group was healthy control group, the second group was positive control group, received a high-fat diet containing 100 mg of cholesterol powder, 25 mg of cholic acid powder and 1 mL of quail egg yolk for 28 days. Third and fourth treatment groups were received for 28 days, plus 0.828 g (1 mL) and 1.656 g (2 mL) of olive oil extract daily for 14 days, respectively. The measurement of total cholesterol used cholesterol test strips based on oxidation enzyme reaction cholesterol esterase and cholesterol oxidase that produce hydrogen peroxidation, then analyzed with biosensor refractophotometry. Measurement of malondialdehyde used thiobarbituric acid (TBA) test. Histological observation of fatty liver was assessed using a NAS (Non-alcoholic fatty liver disease score). The results showed that 1.656 g (2 mL) of olive oil extract per day decreased total cholesterol level up to 44.41 %, malondialdehyde level up to 61.75%, and NAS score up to 50%, compared with positive control. It was concluded that olive oil extract was decreasing total cholesterol level, as an anti-oxidant and prevent fatty liver development.Keywords: fatty liver, hyperlipidemia, malondialdehyde, Olea europaea.
Identification of Microplastics in The Outer Ambon Bay, Mollucas Lekatompessy, Vanela Chatrin; Marhendra, Agung Pramana Warih; Kurniawan, Nia
The Journal of Experimental Life Science Vol. 14 No. 2 (2024)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2024.014.02.02

Abstract

Marine plastic debris that enters the sea can be fragmented by physical and chemical factors, then float in the water column or accumulate in sediments, which have the potential to be ingested by marine biota, causing digestive system disorders, fecundity, eating capacity, reproduction, and death. This study aimed to identify the accumulation of microplastics in water and sediments in Outer Ambon Bay using a purposive sampling method and descriptive analysis for data related to microplastics' type, color, and size, as well as follow-up tests of the least significant difference. The results show that the waters of Outer Ambon Bay have been polluted by microplastics. The number of microplastic particles found in water samples at three different depths (0, 50, and 100 cm) was 201 particles L-1, and in sediment samples, it was 325 particles g-1. It is supported by the fact that the type of fiber has a thin shape and size and can float on the surface of the water and by the existence of beaches where there are fishing activities, boat ports, rivers, and densely populated areas, as well as sandy and muddy sediments that can trap more microplastics during the tidal period. Therefore, it is necessary to prevent the waste problem in the waters of Ambon Bay. Keywords: Identification, Marine Pollution, Microplastics, Outer Ambon Bay.
Sustainability Analysis of Milkfish Farming in Traditional Ponds in Blandongan Village, Bugul Kidul Sub-District, Pasuruan City Anggraeni, Ike Listya; Kartikaningsih, Hartati; Marhendra, Agung Pramana Warih
ECSOFiM (Economic and Social of Fisheries and Marine Journal) Vol 12, No 1 (2024): ECSOFiM October 2024
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.ecsofim.2024.012.01.07

Abstract

Pasuruan City, located in East Java, plays a significant role in the fisheries sector, contributing to industrial and trade activities. However, production declines, influenced by poor water quality and flooding, threatens the sustainability of jelak milkfish farming. This study aims to analyze the status and sustainability index of the milkfish farming business using the MDS-Raps method, which evaluates it based on five dimensions: ecology, economy, social aspects, technology, and institutions. The respondents included 41 farmers from four fish farming groups (Pokdakan). The results shows that the business is fairly sustainable, with a multidimensional index of 51.99, meaning it can be continued and developed but does still has room for improvements. The sustainability status by dimension is as follows: ecological (65.24, fairly sustainable), economic (37.39, less sustainable), social (78.72, very sustainable), technological (27.40, less sustainable), and institutional (51.18, fairly sustainable). The study also proposes seven strategies to enhance sustainability and identifies a link between the MDS analysis and SWOT strategies in managing milkfish farming. Each SWOT strategy offers insights for improving the value of individual dimensions in the MDS framework.
Assessing the Genotoxicity Effect of a Commercial Chlorpyrifos Formulation in Fejervarya limnocharis Tadpoles (Anura: Dicroglossidae) Under Acute and Chronic Exposure Ramadani, Shima; Marhendra, Agung Pramana Warih; Kurniawan, Nia
The Journal of Experimental Life Science Vol. 12 No. 1 (2022)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2022.012.01.02

Abstract

The potential for genotoxicity of pesticides is currently one of the world's concerns. Chlorpyrifos is the organophosphate active ingredient with the largest sales, but the potential for genotoxicity in amphibians is still not widely known. The purpose of this study was to assess the genotoxicity effect of a commercial chlorpyrifos formulation Dursban 200EC in F. limnocharis tadpole erythrocyte (Anura: Dicroglossidae) under acute and chronic exposure using by micronucleus assay. Acute and chronic toxicity tests consisted of negative control, positive control, and 0.4, 0.8, and 1 µg.L-1 of chlorpyrifos with three replications. A toxicity test was carried out on ten tadpoles (Gosner 25) from artificial reproductions in each treatment. The results showed that the formulation of Dursban 200EC in low concentrations (0.4 µg l-1) had the potential to induce DNA damage in erythrocytes of F. limnocharis tadpoles, and there was a positive correlation between chlorpyrifos concentrations and an increase in the frequency of MN. Erythrocytes exposed to chlorpyrifos in both acute and chronic treatment had significantly different MN frequencies between negative and positive controls, 0.4, 0.8, and 1 µg.L-1 (p<0.01). Meanwhile, positive controls were not significantly different from 1 µg.L-1 ( p>0.05). However, the increase in the frequency of MN in chronic treatment was almost twice as high.
Effect of Active Detergent Ingredients on Successful Fertilization and Embryo Development of Sea urchins Tripneustes gratilla (Linnaeus, 1758) Molle, Aprilia Paskarani; Marhendra, Agung Pramana Warih; Sri Rahayu
The Journal of Experimental Life Science Vol. 12 No. 2 (2022)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2022.012.02.04

Abstract

The success of fertilization and development of sea urchin embryos Tripneustes gratilla can be used as a bioindicator of the water quality against the accumulation of pollutants. One of the contaminants that are often used is detergent with an active ingredient in the form of LAS. The purpose of this study was to analyze the effect of LAS on the success of fertilization and development of sea urchin embryos. This research was conducted in the Zoology Laboratory, Pattimura University, Ambon. Tripneustes gratilla were treated with exposure to the active ingredient LAS with a concentration of 0.0; 0.5; 1, and 2 mg.L-1. The parameter used to assess the success of fertilization is the formation of membrane fertilization. Parameters of embryo development are the division of 2, 4 , 8 , 16 , and 32 cells to form a morula, blastula, and hatching blastula. The results showed that the active ingredient of detergent LAS with a concentration of 0.5 mg.L-1 caused delays in the process of fertilization and embryo development. Meanwhile, the active ingredient LAS with concentrations of 1 and 2 mg.L-1 caused failure in the fertilization process and the embryonic development process of T. gratilla. Keywords: Detergent, embryo, fertilization, Tripneustes gratilla.
Effect of Curcumin (Curcuma xanthorriza) and Red Ginger (Zingiber officinale var. rubrum) Ethanol Extract on Improvement of Mice Sperm Quality Exposed by Monosodium Glutamate Aprilia, Niken Herny Lidya Aprilia; Rahayu, Sri; Marhendra, Agung Pramana Warih
The Journal of Experimental Life Science Vol. 14 No. 1 (2024)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2024.014.01.03

Abstract

Temulawak (Curcuma xanthorriza) and red ginger (Zingiber officinale var. rubrum Theilade) contain antioxidant compounds that play an important role in inhibiting the negative effects of the excessive administration of free radicals. Excessive free radicals affect the spermatogenesis process. Which results in decreased sperm quality. This research determined the effect of administering ethanol extract of ginger and red ginger on the sperm quality of mice exposed to MSG. This study used 25 male mice (Mus musculus), aged 2.5 – 3 months and weighing 25 – 30 g, which were randomly divided into five groups: P0 (control), P1 MSG 4 mg.g-1 bw, P2 MSG 4 mg.g-1 bw and C. xanthorriza extract 0.2 mg.g-1 bw, P3 MSG 4 mg.g-1 bw and Z. officinale extract 0.4 mg.g-1 bw, P4 4 mg.g-1 bw, and a combination of C. xanthorriza extract 0.1 mg.g-1 bw and Z. officinale extract 0.2 mg.g-1 bw, MSG, and all extracts were administered orally for 30 days. On the 31st day, the mice were dissected, and epididymis was collected for sperm quality analysis, such as motility, viability, abnormality, and spermatozoa concentration. Subsequently, the sperm quality data were analyzed using One-way ANOVA through the SPSS 16.0 program for Windows (P<0.05), followed by Tukey's Honestly Significant Difference (HSD) test. The addition of both single and combination from C. xanthorriza and Z. officinale can ameliorate motility, viability, and spermatozoa compared with the group that was only given by MSG. Thus, adding temulawak (C. xanthorriza) and red ginger (Z. officinale) ethanolic extract can also ameliorate the mice's sperm quality. Keywords: C. xanthorriza, MSG, sperm quality, Z. officinale
Effectiveness of Extract Supplements (Apium graveolens L.) to Estrogen Receptors Expression and Oocyte Diameter at Nucleolar Chromatin and Perinucleolar Stages in Female of Nile tilapia (Oreochromis niloticus L.) Rikardo, Novy Kurnia; Marhendra, Agung Pramana Warih; Kurniawan, Nia; Riawan, Wibi; Rahayu, Sri
The Journal of Experimental Life Science Vol. 13 No. 3 (2023)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2023.013.03.03

Abstract

The study of the use of celery (Apium graveolens L.) in the health sector has been widely discussed. The analysis of the use of celery in the process of oocyte development still needs to be addressed. This study was conducted to see the effect of celery extract on the expression of estrogen receptors on the gonads and oocyte diameter in tilapia for the first time. Fish are divided into four groups: P0 = Control treatment, feed without mixture; P1 = Treatment with celery extract concentration of 2,000 mg.kg-1 of feed; P2 = Treatment with celery extract concentration of 4,000 mg.kg-1 of feed; P3 = Treatment with celery extract concentration of 6,000 mg.kg-1 of feed. The results showed celery extracts expressed estrogen receptors on the gonads. The expressed estrogen receptor is the estrogen receptor β, whereas the estrogen receptor α is not detected in the gonad. Group statistical tests ANOVA showed no significant results in the development of oocytes. On the other hand, celery extract also impacted the development of oocytes at the nucleolar stage of chromatin, with a markedly greater oocyte diameter. Meanwhile, in the perinucleolar stage, celery extract reduces the oocyte diameter. Keywords: Apium graveolens L, ERα, ERβ, nucleolar chromatin, perinucleolar.
Revisi Peta Distribusi Ikan Padi (Adrianichthyidae: Oryzias spp.) Berdasarkan Identifikasi Morfologi di Paparan Sunda Indonesia Paricahya, Akhsan Fikrillah; Kholil, Kiki Nur Azam; Sufaichusan, Ifa; Herjayanto, Muh.; Saputri, Rengga Retno Laila; Faqih, Abd. Rahem; Marhendra, Agung Pramana Warih
Jurnal Penelitian Pendidikan IPA Vol 10 No 5 (2024): May
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i5.6758

Abstract

Members of the Adrianichthyidae family share typical distributions, as does the javanicus group, which inhabits western parts of Southeast Asia. Javanicus group members, specifically Oryzias javanicus and Oryzias hubbsi, is the only Adrianichthyidae species that can be spotted in Sundaland Indonesia. Morphological identification and the species distribution mapping of Oryzias specimens from Sundaland in this study were interpreted using ecoregional and dendogram tree approach. Specimens were collected from 11 areas representing five ecoregions in Sundaland Indonesia. Specimens were collected from islands of Java, Sumatra, Belitung and Kalimantan. Six individuals from each population of Oryzias were sampled for morphological identification. Specimens collected from estuaries were morphologically identified as O. javanicus and specimens from freshwater identified as O. hubbsi. Distribution of O. hubbsi from Salatiga is the first record from Central East Java (CEJ) ecoregion, which was previously only distributed in Southern Sumatra-Western Java (SWJ) ecoregion. Morphology of O. hubbsi from Sundaland Indonesia has mean of Standard Length (SL) 1.56 cm, Total Length (TL) 1.94 cm, Anal rays (A) 16, Pectoral rays (P) 8, and Dorsal rays (D) 6. Meanwhile, morphology of O. javanicus from Sundaland has mean of SL 2.07 cm, TL 2.56 cm, A 22, P 11, and D 6.
Co-Authors Abdul Rahem Faqih Anggraeni, Ike Listya Aprilia, Niken Herny Lidya Aprilia Aulanni'am, Aulanni'am Aulanni'am, Aulanni'am Darojat, Annisa Zakiyah Dewa Gede Raka Wiadnya Firmawati, Aulia Hanifwati, Annisa Herjayanto, Muh. Kartikaningsih, Hartati Kholil, Kiki Nur Azam Lekatompessy, Vanela Chatrin Molle, Aprilia Paskarani Nia Kurniawan Noer Muhammad Dliyaul Haq, Noer Muhammad Dliyaul Paricahya, Akhsan Fikrillah Ramadani, Shima Rikardo, Novy Kurnia Sangen, Olea Rody Saputri, Rengga Retno Laila SRI RAHAYU Sufaichusan, Ifa Titisari, Nurina Wibi Riawan