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All Journal Jurnal Kedokteran Brawijaya The Journal of Experimental Life Sciences (JELS) Biotropika ECSOFiM (Economic and Social of Fisheries and Marine Journal) JFMR (Journal of Fisheries and Marine Research) Jurnal Medik Veteriner
Agung Pramana Warih Marhendra, Agung Pramana Warih
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Engineering Environmental Science Immunology & microbiology Medicine & Pharmacology Neuroscience Social Sciences Veterinary

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Detection of Reproductive Status in Ongole Crossbred (PO) Cow Based On Vaginal Epithel Morphology and Profile Hormone Widyaningrum, Yeni; Aulanni'am, Aulanni'am; Marhendra, Agung Pramana Warih
The Journal of Experimental Life Science Vol. 10 No. 1 (2020)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (672.446 KB) | DOI: 10.21776/ub.jels.2019.010.01.05

Abstract

Hormonal fluctuations in livestock will affect vaginal cytology good overview on the condition of estrous until pregnancy. The purpose of this study was to determine the physiological condition of Ongole crossbred (PO) cow during estrous and determine pregnancy by the description of vaginal epithelial cells, progesterone, and estrogen hormone profiles. The materials were used 35 cows with physiological status (estrous, 5th pregnancy period, 16th pregnancy period, 22nd pregnancy period, and 60th pregnancy period). Samples of Vaginal smear were stained with Giemsa, then it was observed using a microscope, with 40 times magnification. The progesterone and estrogen were analyzed by the ELISA method. The parameters measured were the percentage of vaginal epithelial cells, such as (parabasal, intermediate, and superficial) started estrous phase until the time of pregnancy in cows (5, 16, 22, and 60 days), hormone concentration, as well as the presence or absence of leukocytes. The result showed the Ongole crossbred cow estrous phase percentage of superficial cells 56.27%±6.49 higher than 26.23%±7.98 intermediate cells, followed by parabasal cells 17.50%±4.74. While in Ongole crossbred that were 5th pregnancy period until the 60th predominantly intermediate cell 80.43%±1.31, then the superficial cells 18.09%±1.30 and 1.48%±0.04 parabasal cells. Progesterone concentration was 63.74±1.07 ng.mL-1 in estrus cows, and steadily increased 93.71±0.94 ng.mL-1 to 149.5±0.71 ng.mL-1 in pregnant cows (5-60 days). The concentration of high estrogen levels were 122.38±0.63 ng.mL-1 in the estrous phase, then decreased 81.54±0.44 ng.mL-1 in the pregnancy phase. In conclusion, the concentration of hormone showed a diagnosis of pregnancy, which done by looking at changes in vaginal epithelial cells at the Ongole crossbred cow, and the cow estrous phase showed greater superficial cells compared by pregnant cows (5-60 days). Keywords: diagnosis of pregnancy, estrous, hormone, Ongole crossbred of cow, vaginal cytology.
Effect of Cold Storage Time (4°C) on Malondialdehyde (MDA) Level, Motility and Viability Spermatozoa of Cyprinus carpio L. Punten Strain Wirastuti, Rosyi; Marhendra, Agung Pramana Warih; Souhaly, Jantje Wiliem; Rahayu, Sri
The Journal of Experimental Life Science Vol. 10 No. 2 (2020)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2020.010.02.08

Abstract

The aim of this research is to know the level of MDA, motility, and viability spermatozoa of C. carpio L. in cold storage time at 4°C. This study used treatment extenders that were NaCl 0.9% as control, diluent of egg yolk with concentrate 0%, 5%, 10%, 15%, and storage in 0 h, 24 h, 48 h, 72 h, and 96 h at 4°C. The data were analyzed using ANOVA (P<0.05). The results showed that the quality (motility and viability) of spermatozoa C. carpio L. punten strain in cold storage could be maintained at 96 h. The optimum storage for motility was found in diluent of egg yolk concentrate 5% at 48 h. MDA levels of semen from C. carpio L. Punten strains after being stored at cold temperatures increased at 0 h to 24 h, then decreased after 48 h of storage and increase at 72 h - 96 h stored. Egg yolk concentration and storage time had a significant effect on MDA levels. There is a negative correlation between MDA levels and motility. There was no correlation between MDA levels and the viability of spermatozoa C. carpio L. punten strain. Keywords: C. carpio L, Egg yolk, Malondialdehyde, Motiliy, Viability
Minnow Trap Color Effectiveness Test Using Cat Food Bait as Aquatic Sampling Gear on Diurnal Fish in Gajah Mungkur Reservoir, Cental Java, Indonesia Arifianto, Adityas; Mufti, Elsa; Marhendra, Agung Pramana Warih; Kurniawan, Nia
The Journal of Experimental Life Science Vol. 11 No. 1 (2021)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.01.04

Abstract

The Minnow Trap is a simple sampling gear, efficient, easy to operate, affordable, easy to make, and has been used in research in the fisheries and aquatic ecology for more than 90 years. The brightness, color, and visual obstruction of fishing gear or fish traps affect the behavioral response of target fish, considering that each fish has a specific level of color sensitivity. The color effectiveness test of fishing gear is an important prerequisite for the use of sampling tools, and it needs to be evaluated to understand the gear performance and avoid potential sampling bias. The purpose of this study was to test the color effect of the minnow trap on diurnal fish in the Gajah Mungkur Reservoir waters. The research was carried out in Gajah Mungkur Reservoir, Wonogiri Regency, at two stations. The sampling conducted at 09.00 - 11.00 and 13.00 - 15.00 Indonesian West Time, where the initial survey is estimated to be the highest time for diurnal fish activity. The minnow trap used in this study is four colors vinyl-painted double funnel cylindrical minnow trap with a 5x5 millimeter mesh size and a conventional umbrella minnow trap used as control. The sampling results are then recorded on the datasheet and analyzed using Microsoft Excel. CPUE (Catch Per Effort Unit) of each unit is then calculated and statistically analyzed by Kruskal-Wallis test and Kolmogorov-Smirnov normality test through SPSS 25.0 software. The test result of five unit minnow traps from 40 installations and two hours of deployment time, 80 individuals were captured, consisted of seven species of freshwater fish. CPUE values ranking consecutively from the highest from silver units (1.375), black (1.25), green (1.125), red (0.9375), and control (0.3125). The Kruskal-Wallis analysis showed that all tested units do not have a significant difference. Keywords: Color, catch per unit effort, fish, minnow trap.
Pola Musim Penangkapan Ikan Pelagis: Studi Kasus Armada Perahu Sekoci dan Slerek di Pelabuhan Perikanan Pantai Pondokdadap: Seasonal Patterns of Pelagic Fish Fisheries: A Case Study of the Sekoci and Slerek Fishing Fleets at Pondokdadap Coastal Fishing Port Anam, M. Choirul; Khamidah, Nur; Yasmin, Delviega Aisyah; Harlyan, Ledhyane Ika; Marhendra, Agung Pramana Warih; Wiadnya, Dewa Gede Raka
JFMR-Journal of Fisheries and Marine Research Vol. 10 No. 1 (2026): JFMR on March
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2026.010.01.9

Abstract

Musim penangkapan ikan didefinisikan sebagai waktu-waktu dalam setahun di mana aktivitas penangkapan bisa menghasilkan ikan target secara optimal. Studi ini bertujuan untuk (1) analisis musim penangkapan terhadap ikan target dari armada perikanan Sekoci (pancing) dengan armada Slerek (purse seine), dan (2) analisis produksi hasil tangkapan nelayan yang beroperasi pada musim barat yang oleh sebagian besar nelayan dianggap sebagai musim paceklik. Penelitian dilakukan pada PPP Pondokdadap pada armada Sekoci dan Slerek. Data hasil tangkapan harian yang diagregasi menjadi bulanan dari kedua armada dikumpulkan dari Januari 2021 sampai Desember 2024. Jenis ikan hasil tangkapan diidentifikasi menggunakan panduan penciri morfologis. CPUE dihitung sebagai hasil pendaratan (kg) per jumlah trip pendaratan (landing trips) dan dianalisis menggunakan Indeks Musim Penangkapan (IMP) berbasis rata-rata bergerak (moving average) terhadap CPUE bulanan. Perbedaan rata-rata CPUE antar bulan diuji menggunakan analisis ragam (ANOVA). Hasil analisis mendapatkan bahwa hasil tangkapan utama dari armada Sekoci ialah tuna oseanik, (madidihang, mata besar, albakora, dan sirip biru), baby tuna dan cakalang dengan ikan non-target (by-catch) marlin (setuhuk/layaran) dan lemadang. Armada Slerek menargetkan ikan pelagis kecil (lemuru dan layang), baby tuna, cakalang, dan tongkol. IMP mengindikasikan bahwa puncak musim relatif terhadap tuna oseanik beserta ikan non-target seperti marlin dan lemadang terjadi pada musim timur (April–September), sedangkan lemuru, layang, baby tuna, dan cakalang cenderung meningkat pada musim barat. Total armada Sekoci yang beroperasi selama musim barat hanya mencapai 5±2,3% dari total armada aktif yang beroperasi, kurang dari setengah armada Slerek (13±1,9%) yang beroperasi selama musim yang sama. IMP menunjukkan kecenderungan musiman relatif, meskipun uji beda rata-rata CPUE antar bulan tidak signifikan (p>0,05) baik pada armada Sekoci maupun Slerek. Tingginya armada Slerek yang beroperasi pada musim barat berhubungan dengan wilayah operasi yang lebih dekat dengan pantai (di dalam wilayah 12 mil), sebaliknya, armada Sekoci melakukan operasi lebih jauh (> 12 mil) dengan mentargetkan tuna oseanik.   Fishing seasons are defined as periods within a year when fishing activities can optimally produce the target catch. This study aims to (1) analyze the fishing seasonality of target species for the Sekoci fleet (handline) in comparison with the Slerek fleet (purse seine), and (2) assess catch production from fishers operating during the west monsoon, which most fishers consider the lean season. The study was conducted at Pondokdadap Coastal Fishing Port (PPP Pondokdadap) focusing on the Sekoci and Slerek fleets. Daily landing records aggregated into monthly data were collected from January 2021 to December 2024. Landed species were identified using morphological identification guides. CPUE was calculated as landed catch (kg) per number of landing trips and analyzed using a Fishing Season Index (FSI/IMP) based on a moving average of monthly CPUE. Differences in mean monthly CPUE were tested using analysis of variance (ANOVA). The results show that the Sekoci fleet’s main catches are oceanic tunas (yellowfin, bigeye, albacore, and bluefin), baby tuna, and skipjack, with non-target by-catches of marlins (sailfish/spearfish) and dolphinfish. The Slerek fleet targets small pelagic fish (sardines and scads), as well as baby tuna, skipjack, and mackerel tuna. The FSI indicates a relative peak season for oceanic tunas and the non-target by-catches (marlins and dolphinfish) during the east monsoon (April–September), whereas sardines, scads, baby tuna, and skipjack tend to increase during the west monsoon. During the west monsoon, the proportion of operating Sekoci vessels averaged 5±2.3% of the active fleet, less than half that of the Slerek fleet (13±1.9%) operating in the same season. The FSI captures relative seasonal tendencies, although differences in mean monthly CPUE were not statistically significant (p>0.05) for either fleet. The higher proportion of Slerek vessels operating during the west monsoon is associated with their nearshore fishing grounds (within 12 nautical miles) and primary target species (sardines, scads, baby tuna, and skipjack). In contrast, the Sekoci fleet-which primarily targets oceanic tunas-is more active during the east monsoon.
Co-Authors Anam, M. Choirul Anggraeni, Ike Listya Annisa Zakiyah Darojat Aprilia, Niken Herny Lidya Aprilia Arifianto, Adityas Aulanni'am, Aulanni'am Aulanni'am, Aulanni'am Dewa Gede Raka Wiadnya Firmawati, Aulia Hanifwati, Annisa Kartikaningsih, Hartati Ledhyane Ika Harlyan Lekatompessy, Vanela Chatrin Molle, Aprilia Paskarani Mufti, Elsa Nia Kurniawan Noer Muhammad Dliyaul Haq, Noer Muhammad Dliyaul Nur Khamidah Ramadani, Shima Rikardo, Novy Kurnia Sangen, Olea Rody Souhaly, Jantje Wiliem SRI RAHAYU Titisari, Nurina Wibi Riawan Widyaningrum, Yeni Wirastuti, Rosyi Yasmin, Delviega Aisyah