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All Journal Dentika Dental Journal Majalah Kedokteran Gigi Indonesia ODONTO Dental Journal Insisiva Dental Journal : Majalah Kedokteran Gigi Insisiva Dental Journal (Majalah Kedokteran Gigi) Jurnal Kedokteran Gigi Universitas Padjadjaran B-Dent, Jurnal Kedokteran Gigi Universitas Baiturrahmah Odonto dental journal Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice)
Rinaldi Budi Utomo
Departemen Ilmu Kedokteran Gigi Anak, Fakultas Kedokteran Gigi, Universitas Gadjah Mada, Yogyakarta, Indonesia
Dentistry Health Professions Medicine & Pharmacology

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Antibacterial effect of ethanol extract of moringa oleifera seeds against enterococcus faecalis atcc 29212 Pratiwi, Ririn Dwi; Kuswandari, Sri; Utomo, Rinaldi Budi
Odonto : Dental Journal Vol 10, No 2 (2023): December 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.2.180-187

Abstract

Background: One of the important stages in endodontic treatment is root canal irrigation. The most widely used irrigant is sodium hypochlorite (NaOCl), but it is toxic to periapical tissue. Irrigant solutions from natural ingredients that have antibacterial potential are needed, one of which is Moringa oleifera seeds. Bacteria that are often found persistent after root canal treatment are Enterococcus faecalis. The purpose of this study was to determine the antibacterial effect of the ethanol extract of M. oleifera seeds at concentrations of 37.5%, 50%, 67.5% and 75% against E. faecalis.Method: Experimental laboratory research with post-test only control group design was carried out with 4 times replication. Antibacterial activity was tested against E. faecalis ATCC 29212 using paper disc diffusion method on Mueller Hinton Agar (MHA) media. The diameter of the inhibition zone formed was measured. Data were analysed by one-way ANOVA followed by LSD.Result: The 2.5% NaOCl group produced the largest inhibition zone of 16.38 ± 0.95 mm, followed by the 75% extract group of 13.51 ± 0.49 mm, and the smallest was the 37.5% extract group of 4.42 ± 0.27 mm. The negative control (DMSO) did not produce an inhibition zone. The increase in the concentration of the ethanolic extract of M. oleifera seeds resulted in significantly better inhibiting the growth of E. faecalis (p<0.05).Conclusion: Ethanol extract of M. oleifera seeds at 37.5%, 50%, 62.5% and 75% had antibacterial effects against E. faecalis, with the 75% had the strongest antibacterial effect compared to other extract concentrations. 
Co-Authors Al Supartinah Dibyo Pramono Dibyo Pramono Emut Lukito Hidayat, Akbar Aulia Indra Bramanti Laelia Dwi Anggraini, Laelia Nendika Dyah Ayu Murika Sari Prathiwi, Caecilia Dewi Ratna Pratiwi, Ririn Dwi Putri Kusuma Wardani Putri Kusuma Wardani Mahendra Rus Sudarso, Iwa Sutardjo SB Sri Rantinah Sri Kuswandari Sri Kuswandari, Sri Sunarno Sunarno Sunarno, Sunarno Syeh Brata Wijaya Titien S, Indah Triani, Maulina Wardhani, Putri Kusuma William Susanto, William