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Analisis Kemampuan Siswa Kelas XI alam Menjawab Soal Berbasis HOTS Pada Materi Sel Siti Djuhriyanti Karim; Ani M. Hasan; Nur Mustaqimah; Abubakar Sidik Katili; Magfirahtul Jannah
Jurnal Pendidikan Biologi Undiksha Vol. 13 No. 2 (2026)
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/jjpb.v13i2.115583

Abstract

Salah satu keterampilan yang sangat diperlukan pada abada 21 adalah kemampuan berpikir tingkat tinggi atau HOTS (Higher Order Thinking Skills), yang mencakup kemampuan untuk menganalisis, mengevaluasi, dan menciptakan. Penelitian ini menganalisis kemampuan siswa SMA Negeri 2 Limboto dalam menyelesaikan soal berbasis HOTS pada materi sel. Penelitian ini menggunakan kuantitatif deskriptif dengan melibatkan 31 siswa kelas XI sebagai subjek penelitian. Instrumen yang digunakan berupa tes HOTS yang terdiri dari enam soal berbentuk esai, yang masing-masing mengukur kemampuan siswa pada level kognitif C4 (menganalisis), C5 (mengevaluasi), dan C6 (mencipta). Hasil penelitian menunjukkan bahwa mayoritas siswa memperoleh nilai yang tergolong rendah, dengan rata-rata skor 35.87 dan standar deviasi 18.39. Daya serap siswa pada level C4 tercatat 41,29%, C5 35,69%, dan C6 23,23%, yang menandakan kesulitan signifikan pada aspek evaluasi dan penciptaan. Temuan ini menunjukkan bahwa meskipun ada sebagian siswa yang menunjukkan kemampuan yang lebih baik, upaya pengajaran yang lebih terstruktur masih diperlukan untuk meningkatkan keterampilan berpikir tingkat tinggi siswa, khususnya dalam evaluasi dan Mencipta.
Distribution and Abundance of Actinomycetes in the Rhizosphere Soil of The Hungayono Karst Ecosystem, Gorontalo Apriliyanti Mohulaingo; Yuliana Retnowati; Abubakar Sidik Katili; Ani M. Hasan; Novri Youla Kandowangko
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.1363-1371

Abstract

Karst ecosystems have unique environmental conditions that can influence the presence of soil microorganisms, including Actinomycetes in the rhizosphere. This study aimed to determine the distribution and abundance of Actinomycetes in the rhizosphere soil of plants in the Hungayono karst ecosystem, Gorontalo. This study employed a quantitative descriptive method with an exploratory approach. Rhizosphere soil samples were collected from several plant species at three observation points: the lower, middle, and upper parts of the karst area. Actinomycetes were isolated using Starch Casein Agar, while their abundance was determined using the Total Plate Count method and expressed in CFU/g of soil. The results showed that actinomycetes were found at all observation points, with abundances ranging from 2 × 103 to 3 × 105 CFU/g. The highest abundances were found in the rhizosphere of Calamus spp., Anthocephalus macrophyllus, and Ficus microcarpa, while the lowest abundances were found in the rhizosphere of Alocasia macrorrhizos. The Actinomycetes isolates obtained exhibited morphological variation based on mycelium color and colony shape. These findings indicate that the distribution and abundance of Actinomycetes in the rhizosphere soil of the Hungayono karst ecosystem vary among plant species and observation sites. The plant rhizosphere in this karst ecosystem functions as a microhabitat that supports the presence of Actinomycetes and is relevant to studies of karst soil microbiology.
Antagonisme Trichoderma sp. terhadap Colletotrichum capsici: Uji In Vitro sebagai Strategi Pengendalian Hayati Penyakit Antraknosa pada Cabai Rawit Triska Wahyuni Dawit; Ani M. Hasan; Jusna Ahmad; Yuliana Retnowati; Febriyanti Febriyanti
Journal of Science, Technology, and Innovation Vol 1 No 2 (2025): December: Inventa: Journal of Science, Technology, and Innovation
Publisher : CV SCRIPTA INTELEKTUAL MANDIRI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.65310/dvr6m616

Abstract

Anthracnose disease is a plant disease caused by the fungus Colletotrichum capsici, the disease is one of the problems in the cultivation of cayenne pepper (Capsicum frutescens). This study aims to test the antagonistic ability of Trichoderma sp. against the fungus Colletotrichum capsici and observe the morphological changes in Colletotrichum capsici colonies exposed to Trichoderma sp in vitro on cayenne pepper plants Capsicum frutescens. The test method was carried out using the dual culture method to determine the percentage of inhibition by studying the interaction between Trichoderma sp. and Colletotrichum capsici colonies on Potato Dextrose Agar culture media. The results showed that Trichoderma sp. fungi can compete for space and resources against Colletotrichum capsici fungi macroscopically by controlling space and resources, and microscopically by producing antibiotics (antimicrobials) that can inhibit the growth of Colletotrichum capsici. The urgency of this research is the need for safe and environmentally friendly disease control efforts. One method that has been widely developed is by utilizing biological agents, such as Trichoderma sp. which is able to inhibit the growth of the pathogen Colletotrichum capsici.