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All Journal Jurnal Analis Medika Biosains (JAMBS) Community Development Journal Bali Medika Jurnal Community Development Journal: Jurnal Pengabdian Masyarakat Medicra (Journal of Medical Laboratory Science/Technology) Indonesia Berdaya Environmental and Toxicology Management (ETM) Journal of Artificial Intelligence and Digital Business SWARNA: Jurnal Pengabdian Kepada Masyarakat Jurnal Ners dan Kebidanan (Journal of Ners and Midwifery)
Andreas Putro Ragil Santoso, Andreas Putro Ragil
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Journal : Jurnal Analis Medika Biosains (JAMBS)

 1 
Platelet Counts with Modified Stains Using PBS Solution pH 6,8 and 7,0 Rahayu, Mita; Nugraha, Gilang; Santoso, Andreas Putro Ragil
Jurnal Analis Medika Biosains (JAMBS) Vol 12, No 1 (2025): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v12i1.352

Abstract

Pemeriksaan hasil hitung trombosit merupakan salah satu pemeriksaan yang banyak dilakukan di laboratorium klinik. Pemeriksaan jumlah trombosit dilakukan dengan cara manual yaitu dengan cara langsung. Pada metode langsung ini dilakukan dengan Improved Neubauer Counter menggunakan Rees Ecker regent. Tujuan penelitian ini adalah untuk melihat perbedaan hasil hitung trombosit antara pereaksi Rees Ecker dengan pereaksi termodifikasi menggunakan pelarut PBS pH 6,8 dan 7,0. Penelitian ini dilakukan terhadap 15 responden dengan menggunakan metode eksperimen dengan teknik simple random sampling. Hasil penelitian ini diperoleh pada kontrol reagen Rees Ecker rata-rata 283.330 sel/mm 3 , pada reagen termodifikasi dengan pelarut PBS pH 6,8 diperoleh hasil sebesar 332.330 sel/mm 3 dan pada reagen termodifikasi dengan PBS pH 7, 0 pelarut dengan hasil rata-rata 321.000 sel/mm 3 . Analisis data diperoleh nilai signifikansi sebesar 0,275 berada diatas p-value >0,05 sehingga hipotesis diterima yaitu H0.
Differences In Counting The Number of Leukocytes Using Cermai Fruit (Phyllantus acidus) Juice and Turk Solution Using The Counting Room Method Effendi, Akhmad Rizal; Nugraha, Gilang; Santoso, Andreas Putro Ragil
Jurnal Analis Medika Biosains (JAMBS) Vol 10, No 2 (2023): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v10i2.324

Abstract

Leukocytes play a role in the body's defense system. Counting the number of leukocytes can be done manually using turk solution. Glacial acetic acid in turk solution functions to lyse erythrocytes but this compound is included in compounds that are carcinogenic. There is a natural ingredient that can lyse blood cells other than leukocytes, namely Cermai fruit. Cermai fruit contains a weak acid, namely citric acid of 39,8% so that it can lyse blood cells other than leukocytes. This study aims to analyze the difference in the number of leukocytes in cermai fruit juice and turk solution. This type of research is experimental with simple random sampling technique. The sample of this research is cermai fruit which will be used to count the number of leukocytes in 10 respondents. The mean number of leukocytes in the control solution was 6.610 cells/mm3, 10% concentration 4.296 cells/mm3, 20% concentration 4.227 cells/mm3, 30% concentration 5.918 cells/mm3 and 100% concentration 5.745 cells/mm3. Data were analyzed using the Kruskal Wallis test and the sig. 0.001 <0,05 so H1 is accepted, which means there is a significant difference, so it is continued with the Post Hoc test. Post hoc test showed that there was no significant difference in the results of leukocyte counts using cermai fruit with concentrations of 30% and 100% compared to the control (p>0,05). Meaning that only the concentrations of 30% and 100% showed the suitability of the number of leukocytes with the control so that these concentrations could be used as an alternative to 3% glacial acetic acid in turksolution.
Co-Authors Afifah, Halimatul Amalia, Ikke Nanda andini, ary Andini, Ary Anggriyani, Rinda Asror, Anita Rizki Rahmawati Devyana Dyah Wulandari Edjianto, Soebagijo Poegoeh Effendi, Akhmad Rizal Endah Budi Permana Putri Ersalina Nidianti Filia, Sisca Gilang Nugraha Heris Santy, Wesiana Kardina, Rizki Nurmalya Kaunain, Ahmad Miftahul Khusnah, Hidayatul Mardhotillah, Rachma Rizqina Mufida, Nisaul Muhammad, Akbar Reza Nila, Hartatiek Nugroho, Mukhtar Adi Nur Masruroh, Nur Prayekti, Endah Rahayu, Mita Rohmah, Wardah Romdloni, Muhmmad Afwan S, Widya Novida Salim, Hotimah Masdan Sari, Nathalya Dwi Kartika Setiarsih, Dini Ulan, Della Citra Wijanarko, Robert Aditya Zazabilla, Indira