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Pratiwi Pujilestari Sudarmono
Departemen Mikrobiologi, Fakultas Kedokteran, Universitas Indonesia, Jakarta
Aerospace Engineering Agriculture, Biological Sciences & Forestry Automotive Engineering Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Electrical & Electronics Engineering Immunology & microbiology Industrial & Manufacturing Engineering Mathematics Medicine & Pharmacology Neuroscience Public Health Veterinary

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Journal : Microbiology Indonesia

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Hospital Acquired Bacterial Infection in Burns Unit at Cipto Mangunkusumo Hospital, Jakarta PRATIWI SUDARMONO; VERONICA WIWING
Microbiology Indonesia Vol. 1 No. 1 (2007): April 2007
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (54.867 KB) | DOI: 10.5454/mi.1.1.6

Abstract

Burn injury causes mechanical disruption to the skin, which allows environmental microbes to invade the deeper tissues. A prospective study of infections in burn patients has shown that the incidence of hospital acquired bacterial infection in burn wounds was high. In the Burns Unit, Cipto Mangunkusumo Hospital, Jakarta, 94 patients were hospitalized from January to July 2004. The objective of this study was to evaluate the hospital acquired infections in burn wounds. Using a cross sectional study, 49 patients were included. The specimens for bacterial investigation were obtained from clean eschar which has healthy tissue taken at day 1, day 5 and day 10. At the same time, bacterial investigations were conducted from the air and the water, as well as from the hand and nasal swabs of hospital personnel. The results show that Klebsiella pneumoniae is the most prominent bacterium found in the wounds, but it is also found in the air. Pseudomonas aeruginosa was the number two causative bacteria which caused a change of the bacterial infectivity on day 5 and 10. These bacteria were always found when we conducted bacterial investigations from the water resource of the burns unit. Methicillin Resistant Staphylococcus aureus is also found in the nasal swab of hospital personnel. Using the antibiogram pattern, there were similarities between bacteria found in the wounds and in bacteria found in the air and water. In conclusion, hospital acquired burn wound infection in Burns Unit, Cipto Mangunkusumo Hospital is as high as 62%. The surveillance data are very important for developing good clinical practice guidelines in burn injury treatment and management
Optimization of Surfactin Production by Bacillus amyloliquefaciens MD4-12 using Response Surface Methodology AHMAD WIBISANA; WAHONO SUMARYONO; MIRAWATI SUDIRO; PRATIWI PUDJILESTARI SUDARMONO
Microbiology Indonesia Vol. 9 No. 3 (2015): September 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (372.202 KB) | DOI: 10.5454/mi.9.3.4

Abstract

Surfactin is a lipopeptide biosurfactant that show potential biomedical application due to its activities such as antiviral, antibacterial, antifungi, anticancer, and antimycoplasma. Bacillus amyloliquefaciens MD4-12, isolated from oil-contaminated soil, produced promising yield of surfactin in McKeen medium. The production of surfactin was influenced by many fermentation process parameters such as carbon, nitrogen, minerals and also environmental conditions such as pH and agitation. Therefore, to obtain high yield of surfactin by Bacillus amyloliquefaciens MD4-12, optimization of process production was conducted in shake flask fermentation using response surface methodology. McKeen medium composition was used as basal medium.  Screening of the best carbon and nitrogen source were selected in preliminary experiments followed by selection of the influencing significant parameters on surfactin production using Plackett-Burman design. Selected parameters were optimized by central composite design and for the data analysis was used response surface methodology. The result showed that the optimum medium composition contained (g/L) 45.0 glucose, 6.33 urea, 1.0 monosodium glutamate, 1.85 MgSO4.7H2O, 0.4 KCl, 0.5 K2HPO4 and 0.5 mL trace elements. The surfactin yield at optimal condition was 1.25 g/L, increased 2.4 times compared to condition prior to optimization. 
Co-Authors . Andriansjah Ade Dharmawan Agus Sjahrurachman AHMAD WIBISANA Amin Soebandrio Andi Yasmon Anis Karuniawati Ariyani Kiranasari Asmarani Kusumawati Augustine Natasha Augustine Natasha Azmier Adib Betty Ernawati Chairunissa T. Rizal Cleopas Martin Rumende Delly Chipta Lestari Dewi, Intan Keumala Diniwati Mukhtar Fera Ibrahim Fera lbrahim Fithriyah Fithriyah Fithriyah Fithriyah HAK HOTTA Helwiah Umniyati Hidetaka Tsuji Holy Arief Ingrid S. Surono Leonardus B.S. Kardono Lola Febriana Dewi Louisa Ivana Utami Mardiastuti H Wahid Mardiastuti Mardiastuti Mirawati Sudiro Myrna Adianti Nie Nie Prawoto Prawoto Rela Febriani Robert Utji Shirly Kumala Susan Maphilindawati Noor Sweety Pribadi Tjahjani M. Sudiro TJAHJANI MIRAWATI SUDIRO Tomohiro Oshibe Triyani Sukarso Tunru, Insan Sosiawan VERONICA WIWING Vivi Lisdawati Wahono Sumaryono Yeva Rosana Yulia R. Saharman Yulia Rosa Saharman