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All Journal Jurnal Teknologi Dan Industri Pangan Jurnal Veteriner Microbiology Indonesia Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Medical Journal of Indonesia The Indonesian Biomedical Journal Jurnal Locus Penelitian dan Pengabdian eJournal Kedokteran Indonesia Journal of Clinical Microbiology and Infectious Diseases

Pratiwi Pujilestari Sudarmono

Departemen Mikrobiologi, Fakultas Kedokteran, Universitas Indonesia, Jakarta

Author-ID : 5611275

Aerospace Engineering Agriculture, Biological Sciences & Forestry Automotive Engineering Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Electrical & Electronics Engineering Immunology & microbiology Industrial & Manufacturing Engineering Mathematics Medicine & Pharmacology Neuroscience Public Health Veterinary

Published : 16 Documents Claim Missing Document

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Articles

Title

Resistance pattern of Mycobacterium tuberculosis to first-line antituberculosis drugs Sudiro, Tjahjani M.; Soebandrio, Amin; Prawoto, Prawoto; Sudarmono, Pratiwi
Medical Journal of Indonesia Vol 9, No 3 (2000): July-September
Publisher : Faculty of Medicine Universitas Indonesia

[no abstract available]

Semi-quantitative dot immunoassay for detection of IgM anti-dengue antibodies in human sera Sjahrurachman, Agus; Ernawati, Betty; Ibrahim, Fera; Mardiastuti, Mardiastuti; Sudiro, Tjahjani M.; Sudarmono, Pratiwi
Medical Journal of Indonesia Vol 9, No 1 (2000): January-March
Publisher : Faculty of Medicine Universitas Indonesia

[no abstract available]

Comparison of two dot immunoassays for diagnosis of dengue infection Sjahrurachman, Agus; Ernawati, Betty; lbrahim, Fera; Mardiastuti, Mardiastuti; Sudiro, Tjahjani Mirawati; Sudarmono, Pratiwi
Medical Journal of Indonesia Vol 9, No 4 (2000): October-December
Publisher : Faculty of Medicine Universitas Indonesia

[no abstract available]

Cytotoxic assay of endophytic fungus 1.2.11 secondary metabolites from Brucea javanica (L) Merr towards cancer cell in vitro Sudarmono, Pratiwi; Utji, Robert; Kardono, Leonardus B.S.; Kumala, Shirly
Medical Journal of Indonesia Vol 15, No 3 (2006): July-September
Publisher : Faculty of Medicine Universitas Indonesia

Cytotoxic assay of secondary metabolite endophytic fungus 1.2.11 from Brucea javanica (L) Merr has been carried out. Brucea javanica fruit collected from Cianjur was used in this experiment. Cytotoxic assay was done on Raji, NS-1, HeLa and Vero cells. The observation was done for 24 hours and also for 48 hours. IC50 was calculated using the Rich and Muench theory. To observe the working mechanism of cytotoxic process, DNA staining with etidium bromide and acridine orange was conducted. The cytotoxic assay of endophytic fungi 1.2.11 showed an IC50 of 58.35 Î¼g/ml, 88.39 Î¼g/ml on Raji cell,; 162.09 Î¼g/ml, 66.24 Î¼g/ml on NS cell; 361.21 Î¼g/ml, 219.97 Î¼g/ml on HeLa cell; and lastly 1075.18 Î¼g/ml, 656.82 Î¼g/ml on Vero cell after 24 and 48 hour incubation respectively. The results of this study showed that secondary metabolite of endophytic fungus 1.2.11 has selective cytotoxic effect towards cancer cell and also showed that it might cause apoptosis in NS-1cell. (Med J Indones 2006; 15:137-44) Keywords: Brucea javanica (L.) Merr, endophytic microbe, Cytotoxic assay, endophytic isolate 1.2.11, apoptosis

Evaluating the use of loop-mediated isothermal amplification (LAMP) method for detection of Mycobacterium tuberculosis in Indonesian clinical isolates Lisdawati, Vivi; Oshibe, Tomohiro; Tsuji, Hidetaka; Sudiro, Tjahjani M.; Adianti, Myrna; Sukarso, Triyani; Arief, Holy; Hotta, Hak; Sudarmono, Pratiwi
Medical Journal of Indonesia Vol 21, No 4 (2012): November
Publisher : Faculty of Medicine Universitas Indonesia

Background: Loop-mediated isothermal amplification (LAMP) is a method already claimed as a simple technique to amplify DNA/ RNA using four to six primers as “a set” from conserved sequence of target gene. In this study we optimize the use of LAMP for detection of Mycobacterium tuberculosis in clinical isolates from Indonesia.Methods: Procedures to perform LAMP were optimized, then the method was applied to 122 archieved samples of DNA’s Mtb from clinical TB patients with Acid Fast Bacilli (AFB) smears positive. The samples were obtained in 2008 from 13 provinces in Indonesia for genotyping study, which then become collections of Center for Biomedical and Basic Technology of Health (CBBTH), NIHRD Indonesia. The optimization tests include sensitivity and specificity tests of several sets primers, which were evaluated using 10-fold serially diluted DNA of Mtb H37Rv and 12 species of Mycobacteria. Three equipments consisted of LAMP turbidimeter, heating block and water bath were compared for its ability in DNA amplification. Detection of M. tuberculosis from clinical isolates used set primers specific for gyrB gene, amplicon was detected with UV fluorescence system.Results: The results showed that the highest sensitivity was obtained using the set primers specific for 16S rRNA and gyrB which could detect 10.0 fg to 1.0 pg genomic DNA of Mtb H37Rv. The set primers specific for gyrB gene was the most specific primers. Application of LAMP using gyrB set primers on Indonesian clinical isolates showed 94.2% (114/121) positivity rate.Conclusion: LAMP method is potentially used in TB diagnosis in Indonesia. (Med J Indones. 2012;21:188-95)Keywords: Loop-mediated isothermal amplification, rim gene, 16S rRNA gene, gyrB gene, Mycobacterium tuberculosis

Co-Authors . Andriansjah Ade Dharmawan Agus Sjahrurachman AHMAD WIBISANA Amin Soebandrio Andi Yasmon Anis Karuniawati Ariyani Kiranasari Asmarani Kusumawati Augustine Natasha Augustine Natasha Azmier Adib Betty Ernawati Chairunissa T. Rizal Cleopas Martin Rumende Delly Chipta Lestari Dewi, Intan Keumala Diniwati Mukhtar Fera Ibrahim Fera lbrahim Fithriyah Fithriyah Fithriyah Fithriyah HAK HOTTA Helwiah Umniyati Hidetaka Tsuji Holy Arief Ingrid S. Surono Leonardus B.S. Kardono Lola Febriana Dewi Louisa Ivana Utami Mardiastuti H Wahid Mardiastuti Mardiastuti Mirawati Sudiro Myrna Adianti Nie Nie Prawoto Prawoto Rela Febriani Robert Utji Shirly Kumala Susan Maphilindawati Noor Sweety Pribadi Tjahjani M. Sudiro TJAHJANI MIRAWATI SUDIRO Tomohiro Oshibe Triyani Sukarso Tunru, Insan Sosiawan VERONICA WIWING Vivi Lisdawati Wahono Sumaryono Yeva Rosana Yulia R. Saharman Yulia Rosa Saharman

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