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UJI FITOKIMIA DAN UJI AKTIVITAS ANTIOKSIDAN (METODE DPPH) DARI DAUN RAMBAI (Baccaurea motleyana Mull. Arg.) Ami, Suyatmi; Saleh, Chairul; Pratiwi, Djihan Ryn
JURNAL ATOMIK Vol 4 No 2 (2019)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

The research about phytochemical test and antioxidant activity test on rambai leaves extract (Baccaurea motleyana Müll.Arg.) using DPPH radical immersion method had been done. This research aims to know kinds of secondary metabolite compounds in rambai leaves extract and level of antioxidant activity in rambai leaf. The result of this research showed that the secondary metabolite in rambai leaf were alkaloids, phenolics, flavonoids, saponins, steroids and triterpenoids. IC50 values obtained in each crude extract, n-hexane fraction, ethyl acetate fraction and residual ethanol fraction were 13.27 ppm, 22.81 ppm, 19.22 ppm and 13.33 ppm respectively. The highest level of antioxidant activity was in crude extracts.
SCREENING OF LIPASE FROM WATER BACTERIA IN BENANGA LEMPAKE DAM, NORTH SAMARINDA AND ITS POTENTIAL AS A DETERGENT ADDITIVE Vera, Hendrica Mini; Astuti, Winni; Pratiwi, Djihan Ryn
JURNAL ATOMIK Vol 8 No 2 (2023)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

Lipase is an enzyme that can hydrolyze triglycerol into glycerol and free fatty acids. The aim of this research is to obtain lipase-producing bacteria and determine the potential of lipase from water bacteria in the water of the Benanga Lempake Dam, North Samarinda for biodetergent applications through stability test of lipase activity against several commercial detergents. Screening of lipase-producing bacteria was carried out on nutrient agar media containing olive oil and Rhodamine B. The results showed that seven isolates were capable of producing lipase. Based on the stability test of lipase on detergent, it can be seen that the activity of lipase decreased by more than 70% at the addition of 1% detergent.
Penurunan Konsentrasi Besi dan Mangan Terlarut pada Air Tanah Muara Lawa Menggunakan Serbuk Kiambang (Salvinia molesta) Widodo, Nanang Tri; Sari, Ika Yekti Liana; Pratiwi, Djihan Ryn
PRIMER : Jurnal Ilmiah Multidisiplin Vol. 2 No. 4 (2024): PRIMER : Jurnal Ilmiah Multidisiplin, Agustus 2024
Publisher : LPPM Institut Teknologi Dan Kesehatan Aspirasi

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Abstract

Salah satu sumber air untuk kebutuhan manusia adalah air tanah. Air tanah berfungsi memenuhi berbagai keperluan rumah tangga, seperti mandi, memasak, minum, dan mencuci. Keberadaan zat besi dan mangan di air tanah telah menjadi masalah yang serius sejak lama, kandungan besi dan mangan dapat menumbulkan rasa dan bau pada air tanah. Penelitian ini bertujuan untuk mengetahui tingkat penurunan konsentrasi besi dan mangan terlarut pada air tanah menggunakan serbuk kiambang (Salvinia molesta) sebagai adsorben. Penurunan konsentrasi besi dan mangan pada air tanah dilakukan dengan cara mengadsorpsi besi dan mangan pada air tanah menggunakan serbuk kiambang teraktifasi. Hasil penelitian menunjukkan konsentrasi besi dan mangan pada air tanah dapat diturunkan berturut-turut sebesar 64,77 % dan 77,67 %.
AKTIVITAS AMILASE PENDEGRADASI PATI MENTAH DARI BAKTERI TANAH TEMPAT PEMBUANGAN AKHIR BUKIT PINANG Putri, Amanda Aulia; Astuti, Winni; Pratiwi, Djihan Ryn
JURNAL ATOMIK Vol 9 No 2 (2024)
Publisher : Jurusan Kimia FMIPA UNMUL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/ja.v9i2.1408

Abstract

Raw starch-degrading amylase (RSDA) is a hydrolytic enzyme that can degrade raw starch into glucose. This study was conducted to obtain raw starch-degrading amylase-producing bacteria from the soil of the Bukit Pinang Landfill, Samarinda City. Isolation of bacteria using the spread plate method. Screening of RSDA-producing bacteria using nutrient media to contain raw starch. Isolation of bacteria obtained 10 single colonies of bacteria. The isolated bacteria were selected for their ability to produce RSDA. All single colonies of bacteria could produce RSDA and their activity was tested quantitatively using the 3,5 Dinitrosalicylic Acid (DNS) method. Bacteria with the TPA 1 colony code had the highest activity of 3.837 U/mL.
ANALISIS KADAR NITROGEN (N) TOTAL DAN BESI (Fe) PADA PENAMBAHAN BIOAKTIVATOR EM4 TERHADAP KOMPOS (SAMPAH KACANG PANJANG-KULIT KACANG KEDELAI) Cahyani, Amelya Putri; Sitorus, Saibun; Pratiwi, Djihan Ryn
JURNAL KIMIA MULAWARMAN Vol 22 No 1 (2024)
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/jkm.v22i1.1111

Abstract

Pengomposan merupakan proses penguraian bahan organik sampah dibawah kondisi-kondisi terkontrol oleh adanya aktivitas mikroorganisme pengurai. Proses pengomposan dilakukan dengan penambahan Effective microorgarnism 4 (EM4). Tahapan penelitian meliputi uji pengaruh waktu pengomposan terhadap suhu dan pH; penentuan variasi optimum bioaktivator EM4 terhadap kadar N-Total dan Fe serta waktu optimum pengomposan berdasarkan kondisi suhu, pH dan warna. Variasi optimum EM4 berdasarkan kadar N-Total pada variasi tanpa penambahan EM4 sebesar 0,4731% dan berdasarkan kadar Fe pada variasi EM4 20 mL sebesar 0,805%. Waktu optimum pengomposan berada pada hari ke-16 dengan pH 7,19-7,50; suhu sebesar 27-28,3°C dan memiliki warna hitam.
RAW STARCH-DEGRADING AMYLASE FROM BACTERIA IN KARANG MUMUS RIVER, SAMARINDA Astuti, Winni; Adawiyah, Rabiatul; Putri, Amanda Aulia; Pratiwi, Djihan Ryn
al Kimiya: Jurnal Ilmu Kimia dan Terapan Vol 11, No 2 (2024): al Kimiya: Jurnal Ilmu Kimia dan Terapan
Publisher : Department of Chemistry, Faculty of Science and Technology, UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/ak.v11i2.39438

Abstract

Raw starch-degrading amylase (RSDA) is an enzyme that has the ability to degrade starch granules into glucose without undergoing gelatinization. This research was conducted to explore and characterize extracellular RSDA-producing bacteria from body water of the Karang Mumus River, Samarinda. RSDA activity was analyzed qualitatively in nutrient agar containing 1% starch granules, bacterial colonies with RSDA activity are clear halos around bacterial colonies after the plates were flooded with iodine solution. Five of the 14 bacterial colonies secreted an RSDA extracellularly. The RSDA enzyme from the 5 bacteria was tested for amylase activity using the Dinitrosalicylic Acid (DNS) method. Bacteria with colony code KM 5 had the highest RSDA activity of 0.332 U/mL.The optimum working conditions for RSDA are at pH 5 and a temperature of 40°C. Identification of bacterial genotypes using the 16S rRNA gene showed that KM5 was Klebsiella sp, referred to as Klebsiella KM5.
PHYTOCHEMICAL AND TOXICITY TEST OF MERUNG EXTRACTS (Coptosapelta tomentosa (Blume) Karolina, Anita; Pratiwi, Djihan Ryn; Akkas, Erwin
JURNAL ATOMIK Vol 3 No 2 (2018)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

This study was conducted to determine the content of secondary metabolites and the toxicity of extracts of Merung various parts (Coptosapelta tomentosa (Blume). The research methods included maceration, phytochemical tests and toxicity tests using the BSLT method. Based on the results of the phytochemical test showed all extracts of Merung parts contained steroids, flavonoids, and phenolics. Saponin is only found in leaf extract. Toxicity tests using the BSLT method showed that stem and root extracts were toxic with LC50 values of 2004.06 and 173.09 ppm, respectively.
PHYTOCHEMICAL TEST AND ANTIBACTERIAL ACTIVITY OF METHANOL EXTRACT OF Melicope lunu-ankenda (Gaertn.) T.G. Hartley STEM AGAINST Salmonella typhi AND Staphylococcus aureus Hermawan, Hermawan; Wirawan, Teguh; Pratiwi, Djihan Ryn
JURNAL ATOMIK Vol 6 No 1 (2021)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

Phytochemical test and antibacterial activity of methanol extract of Melicope lunu-ankenda (Gaertn.) T.G. Hartley have been done. The extraction of Melicope lunu-ankenda (Gaertn.) T.G. Hartley stem sample was carried out by the maceration method using methanol as a solvent. An antibacterial activity test was carried out using the agar diffusion method. Phytochemical tests results showed that methanol extract of Melicope lunu-ankenda (Gaertn.) T.G. Hartley stem has secondary metabolites including alkaloids, steroids, triterpenoids, and quinones. The antibacterial activity test results obtained MIC values for Salmonella typhi bacteria of 1.25% and for Staphylococcus aureus bacteria of 0.625%. Methanol extract of Melicope lunu-ankenda (Gaertn.) T.G. Hartley stem has broad-spectrum antibacterial activity.
SCREENING AND PHYTOCHEMICALS TEST OF EXTRACT ENDHOPHYTES BACTERIA FROM STEM OF PACING (Costus sp.) Aristina, Reza Fauziah; Astuti, Winni; Pratiwi, Djihan Ryn
JURNAL ATOMIK Vol 4 No 1 (2019)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

Pacing plants (Costus sp.) Potentially as medicinal plants, but there is no research on the endophytic bacteria they contain. This research was conducted to filter endophytic bacteria from Pacing stems (Costus sp.) And to find out the secondary metabolites contained by each endophytic bacteria. Endophytic bacteria obtained from Pacing Stems (Costus sp.) Totaled 17 colonies. Crude extracts from all bacterial colonies containing alkaloids, 15 crude extracts containing saponins. Steroids are only contained by 3 types of bacterial extracts and triterpenoids are only contained by 2 types of bacterial extract.
SKRINING FITOKIMIA DAN BIOAKTIVITAS EKSTRAK AKAR Uncaria nervosa Elmer (BAJAKAH) Maulina, Sheli; Pratiwi, Djihan Ryn; Erwin, Erwin
JURNAL ATOMIK Vol 4 No 2 (2019)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

One type of Bajakah that is believed by local people especially in the area of Muara Badak, KutaiKertanegara Regency, has the efficacy of anticancer drugs, is Uncaria nervosa Elmer. The purposes of this studywere to determine the content of secondary metabolites and the toxicity of the extracts of the bark and root of thewood of Bajakah (U. nervosa). From the results of phytochemical tests showed that both bark and root woodcontain secondary metabolites of alkaloids, flavonoids, terpenoids, and phenolics. Moreover, based on the result ofthe mortality test for Artemia salina shrimp larvae, it showed that both the bark and wood of the roots are verytoxic with LC50 values of 1.76 and 2.66 ppm, respectively. Both of these extracts are potentially cytotoxic to cancercells.