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All Journal Indonesian Journal of Biotechnology Universa Medicina International Journal of Cell and Biomedical Science Indonesian Journal of Biomedicine and Clinical Sciences Journal of Midwifery and Health Science of Sultan Agung Indonesian Journal of Pharmacology and Therapy
Nurul Hidayah
Stem Cell And Cancer Research Laboratory, Semarang, Indonesia
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health

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Phyto therapeutic potential of Andrographis paniculata and Catharanthus roseus extract against colorectal cancer HCT-116 cell line Rifai , Fauziah Novita Putri; Hidayah, Nurul; Prabowo, Adam; Pradani, Lisa Nahdalia
Indonesian Journal of Pharmacology and Therapy Vol 6 No 3 (2025)
Publisher : Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada and Indonesian Pharmacologist Association or Ikatan Farmakologi Indonesia (IKAFARI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijpther.24709

Abstract

Colorectal cancer (CRC) is the third most common cancer globally and the second leading cause of cancer-related mortality, with over 1.9 million new cases and 935,000 deaths reported in 2020. Despite therapeutic advances, recurrence, drug resistance, and systemic toxicity remain major challenges. Natural products withantioxidant and cytotoxic activity are increasingly investigated as complementary therapies. Andrographis paniculata (Sambiloto), rich in andrographolide, exerts anticancer effects by inducing apoptosis, inhibiting migration and invasion, and modulating PI3K/Akt and NF-κB signalling pathway. Catharanthus roseus (TapakDara) produces vinca alkaloids, including vincristine and vinblastine, which inhibit microtubule polymerization and are widely used in chemotherapy. Combining these extracts may enhance efficacy and reduce toxicity through synergistic interactions. This in vitro study assessed the cytotoxic and synergistic effectsof A. paniculata extract (APE) and C. roseus extract (CRE) on HCT-116 colorectal cancer cells. Extracts were prepared by ethanol maceration, and cytotoxicity was evaluated using the MTT assay at concentrations ranging from 5 to 100 μg/mL. IC₅₀ values were calculated using linear regression, and the combination index(CI) was determined at 1, 1/2 and 1/4 IC₅₀ to evaluate synergism. APE and CRE exhibited comparable cytotoxicity, with IC₅₀ values of 90 μg/mL and 89.5 μg/mL, respectively. The combination treatment revealed synergistic effects (CI <1) at multiple ratios, particularly at 1/4 IC₅₀ (CI = 0.58), demonstrating enhancedcytotoxicity at reduced concentrations. Both APE and CRE demonstrated significant cytotoxic effects against HCT-116 cells. Their combination produced synergistic interactions, suggesting potential as complementary phytotherapeutic agents for CRC with the benefits of dose reduction and minimized toxicity. Further in vivo and mechanistic studies are warranted.
Modulation of inflammatory pathways in ischemic stroke rats by hypoxia-primed umbilical cord mesenchymal stem cells (UC-MSCs): Implications for IFN-γ and IL-10 signaling Fahreza, Rakha; Setiawan, Eko; Trisnadi, Setyo; Putra, Agung; Hidayah, Nurul; Fikriya Novita Sari; Ibrahim, Sugeng
Indonesian Journal of Biomedicine and Clinical Sciences Vol 57 No 4 (2025)
Publisher : Published by Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/inajbcs.v57i4.24529

Abstract

Ischemic stroke is a major global cause of disability and mortality. Inflammation plays a central role in its pathogenesis, characterized by elevated pro-inflammatory cytokines such as IFN-γ and reduced anti-inflammatory cytokines like IL-10. Mesenchymal stem cells (MSCs), especially those derived from the umbilical cord (UC-MSCs), exhibit enhanced immunomodulatory potential when preconditioned under hypoxia. This study aims to evaluate the effect of hypoxic-preconditioned UC-MSCs administration on IFN-γ and IL-10 levels in an ischemic stroke rat model. This in vivo experimental study employed a randomized posttest-only control group design with four groups of male Wistar rats (n=6 each), ranging from healthy controls, untreated group to stroke-induced groups treated with hypoxic UC-MSCs at different doses (1.5x106 and 3x106). IFN-γ and IL-10 levels in brain tissue of each group were measured via ELISA. Significant reduction in IFN-γ and elevation in IL-10 were observed in UC-MSC-treated groups, particularly at the 3×10⁶ cell dose compared to the untreated ischemic group (p<0.05). Hypoxic UC-MSCs reduce post-stroke inflammation by lowering IFN-γ and enhancing IL-10, indicating a promising immunomodulatory potential.
Non-contact electro capacitive cancer therapy (ECCT) modulate the mRNA expression of p53, Apaf-1, survivin, NF-κB, TSP-1 and bFGF in DMBA-induced breast cancer rat Hidayah, Nurul; Putra, Agung; Alamsyah, Firman; Pratiwi, Rarastoeti
Indonesian Journal of Biomedicine and Clinical Sciences Vol 58 No 1 (2026)
Publisher : Published by Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/inajbcs.v58i1.24954

Abstract

Breast cancer is the most common cancer that causes death in women in the world. Cancer development is facilitated by the inhibition of apoptosis and induction of angiogenesis. Current cancer therapy still encounters problems in the form of recurrence, resistance, and side effects of drugs. Non-contact static electric field therapy, electro capacity cancer therapy (ECCT) with medium frequency, is a therapy developed to inhibit the proliferation of tumor cells. This study aimed to determine the mRNA expression of p53, Apaf-1, survivin related to apoptosis and NF-κB, bFGF and TSP-1 related to angiogenesis in rat breast tumor tissue after ECCT frequency of 150 kHz. Breast tissue samples and rat breast tumor nodules stored in RNA later at -20°C were used. The tissue was obtained from the non-induction non-therapy (NINT) group, induction non-therapy (INT), non-induction therapy (NIT), and induction therapy (IT). mRNA expression of p53, Apaf-1, NF-κB, bFGF and TSP-1 were analyzed using qRT-PCR and calculated with the Livak formula. Data were analyzed using one-way Anova and post-hoc LSD. The results showed that, mRNA expression of p53, Apaf-1 and TSP-1 in the IT group increased significantly, and mRNA expression of survivin and bFGF decreased significantly compared to the INT group. However, the expression of NF-κB mRNA in the IT group remained the same as in the INT group. In conclusion, ECCT with a frequency of 150 kHz upregulates p53, Apaf-1 and TSP-1 mRNA expression and downregulates survivin and bFGF mRNA expression but have no effect on NF-κB mRNA expression in rat breast tumor tissue.
Co-Authors Adityani, Resanti Agung Putra Agung Putra Agung Putra Agung Putra Annisa, Dyah Retno Aprilia, Waheni Rizki Ardi Prasetyo Ari, Psn Masruri Sulistiyanto Cahyani, Dini Chodijah Chodijah Christine Magdalena Dedy Hermansyah Eko Setiawan Fahreza, Rakha Faizal Mukharim Fikriya Novita Sari Firman Alamsyah Ginanto, Dede Ibrahim, Sugeng Iffan Alif Irawan, Risky Chandra Satria Joko Wahyu Wibowo Mohammad Ariq Nazar Muhar, Adi Muradi Nur Anna Chalimah Sadyah Nur Dina Amalina Olifiarsy Wiet Ramadhanti Prabowo, Adam Pradani, Lisa Nahdalia Prasetyowati Prasetyowati Subchan Rachellicya Ristioni Sihaloho Rarastoeti Pratiwi Rifai, Fauziah Novita Putri Rizka Sofyanti Putri Sakinah, Feby Nur Sari, Fikriya Novita Setyo Trisnadi Shabrina Syifa Ghaissani Siti Thomas Zulaikhah Titiek Sumarawati Wilaksono, Bhirau