Article Per Year (5 Year)
p-Index From 2020 - 2025
0.444
P-Index
This Author published in this journals
All Journal Jurnal Gizi dan Pangan Media Penelitian dan Pengembangan Kesehatan Universa Medicina IJMS - Indonesian Journal on Medical Science
Frans Dany, Frans
Pusat Biomedis dan Teknologi Dasar Kesehatan Jl. Percetakan Negara 23 Jakarta 10560, Indonesia
Decision Sciences, Operations Research & Management Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

Published : 4 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 4 Documents
Search

 1 
Seroprevalensi Hepatitis C pada Populasi Perkotaan dan Perdesaan di Indonesia Tahun 2013: Kajian Determinan Sosiodemografi, Lingkungan, Pejamu, dan Komorbiditas (Analisis Lanjut Riskesdas 2013) Dany, Frans; Handayani, Sarwo
Media Penelitian dan Pengembangan Kesehatan Vol 27, No 4 (2017)
Publisher : Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (0.001 KB) | DOI: 10.22435/mpk.v27i4.6267.197-208

Abstract

Hepatitis C is an infectious disease caused by RNA virus that is often asymptomatic and can lead into serious complications such as liver cancer. It remains one of major public health issues in several developing countries. Urban and rural areas have different sociodemographic characteristics but seroprevalence of hepatitis C based on antibody against hepatitis C virus (HCV) shows the same proportion of 1.0% in both areas, suggesting that there is discrepancy of risk factors for HCV infection between the two. This analysis aims to identify such determinants that include sociodemographic factors, neighbourhood, environmental conditions including hygienic behaviour and access to healthcare facilities, host conditions and comorbidities outside major risk factors. The analysis used secondary data of Riskesdas 2013 through complex logistic regression technique and alternative tests. The results showed that the determinants of HCV seroprevalence in urban and rural respondents were: gender, unemployment status, prediabetes, abnormalities of HDL and triglyceride levels, renal impairment and malaria. Hence, hepatitis C management strategies in Indonesia may require different approach to urban populations compared to rural areas. Abstrak Hepatitis C merupakan penyakit infeksius akibat virus RNA yang sering tidak bergejala dan dapat menimbulkan komplikasi serius seperti kanker hati. Hal tersebut masih menjadi salah satu masalah kesehatan masyarakat utama di sejumlah negara berkembang. Area urban dan rural memiliki perbedaan karakteristik sosiodemografi, tetapi seroprevalensi hepatitis C berdasarkan antibodi terhadap virus hepatitis C (hepatitis C virus, HCV) di Indonesia berdasarkan hasil Riset Kesehatan Dasar (Riskesdas) 2013 menunjukkan proporsi yang sama, yaitu 1,0% sehingga memunculkan dugaan adanya perbedaan faktor risiko infeksi HCV antara kedua kategori tempat tinggal. Analisis ini bertujuan mengidentifikasi determinan tersebut yang meliputi faktor sosiodemografi, kondisi lingkungan sekitar berikut perilaku higienis dan akses ke fasilitas pelayanan kesehatan, kondisi pejamu, serta komorbiditas di luar faktor risiko utama. Analisis menggunakan data sekunder Riskesdas 2013 melalui teknik complex samples regresi logistik dan uji alternatif. Hasil analisis menunjukkan perbedaan determinan seroprevalensi HCV pada responden perkotaan dan perdesaan, antara lain: jenis kelamin, status pengangguran kepala keluarga, prediabetes, abnormalitas kadar HDL dan trigliserida, gangguan ginjal, dan malaria. Karena itu, strategi penatalaksanaan hepatitis C di Indonesia memerlukan pendekatan yang berbeda untuk populasi perkotaan dibandingkan dengan perdesaan.
Efek dimethyl sulfoxide (DMSO) terhadap Karakteristik Sel Punca Limbal (SPL) Tikus Rinendyaputri, Ratih; Dany, Frans; - Pusat Biomedis dan Teknologi Dasar Kesehatan Balitbangkes, Kemenkes RI, Uly Alfi Nikmah
IJMS - Indonesian Journal on Medical Science Vol 5, No 1 (2018): IJMS 2018
Publisher : IJMS - Indonesian Journal on Medical Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (404.374 KB)

Abstract

Abstact : The number of donor limbal stem cells (LSCs) is limited despite their large demand for management of LSC deficiency-related corneal opacity, requiring propagation of these cells in vitro. Production of LSCs can be done through isolation and culture of limbal tissue in vitro and cryopreservation of LSCs is utilized to maintain the availability of LSCs. Upon cryopreservation, cryoprotectants are required to protect cells from thermal injury. Dimethyl sulfoxide (DMSO) is a commonly used cryoprotectant for cryopreservation but the effect of its use on LSCs are still seldomly reported. This study aimed to determine the effect of the use of DMSO in cryopreservation of LSC. The study was conducted at the Stem Cell Laboratory, Center for Biomedical and Basic Technology of Health, NIHRD Ministry of Health. This research was performed by culturing and observation of  LSCs characteristic after cryopreservation. Meanwhile, level of LSC proliferation was determined by calculating population doubling (PD) and population doubling time (PDT) besides analyzing their gene expression using markers such as CD90 (Thy1) and Krt12. The results showed that PD and PDT in LSC control and post-cryopreservation without DMSO and cryopreservation using DMSO accordingly are 1.33 and 143.03, 150.65 and 1.15, and 1.31 and 155. Meanwhile, CD90 (Thy1) gene expression and Krt12 expression in the cryopreserved group with and without DMSO compared with their respective controls are 2.7 and 4.51, 2.55 and 1:44, respectively. In this study, DMSO for the cryopreservation did not affect at the LSC characteristics of rat.Key word : limbus stem cell, LSC, cryopreservation, dimethyl sulfoxide, DMSO Abstrak : Jumlah donor sel punca limbal (SPL) sangat terbatas padahal kebutuhannya cukup besar untuk penatalaksanaan kekeruhan kornea akibat defisiensi sel tersebut sehingga SPL perlu diperbanyak secara in vitro. Produksi SPL secara in vitro dapat dilakukan dengan melakukan isolasi dan kultur dari jaringan limbal, dan metode simpan beku atau kriopreservasi SPL digunakan untuk menjaga ketersediaan SPL. Pada saat kriopreservasi, dibutuhkan krioprotektan yang dapat melindungi sel dari kerusakan termal saat kriopreservasi. Dimethyl sulfoxide (DMSO) merupakan salah satu krioprotektan yang umum digunakan untuk kriopreservasi namun efek penggunaan pada SPL masih sangat jarang dilaporkan. Penelitian ini bertujuan untuk mengetahui efek penggunaan DMSO pada kriopreservasi SPL. Penelitian dilakukan di Laboratorium Stem Cell Pusat Biomedis dan Teknologi Dasar Kesehatan Badan Litbangkes. Penelitian ini dilakukan dengan melakukan kultur SPL tikus menggunakan metode eksplan secara in vitro pada cawan petri. Pengamatan terhadap karakteristik SPL pasca kriopreservasi dilakukan dengan pengamatan terhadap morfologi SPL secara mikroskopis dan mengetahui tingkat proliferasi SPL dengan menghitung population doubling (PD) dan population doubling time (PDT) serta menganalisis ekspresi gen CD90 (Thy1) dan Krt12 sebagai marker SPL. Hasil penelitian menunjukkan bahwa PD dan PDT pada SPL kontrol dan pasca kriopreservasi tanpa DMSO dan kriopreservasi dengan DMSO secara berturut-turut adalah 1.33 dan 143.03, 1.15 dan 150.65 serta 1.31 dan 155. Sedangkan tingkat ekspresi gen CD90 (Thy1) dan Krt12 SPL pada penggunaan dan tanpa DMSO dibandingkan dengan kontrol masing-masing adalah 2,7 dan 4,51 serat 2,55 dan 1,44 kali. Pada penelitian ini, DMSO tidak  mengubah  karakteristik SPL tikus. Kata kunci: sel punca limbal, SPL, kriopreservasi, dimethyl sulfoxide, DMSO
Determinants of diabetes comorbidities in Indonesia: a cohort study of non-communicable disease risk factor Kristanti, Dewi; Rahajeng, Ekowati; Sulistiowati, Eva; Kusumawardani, Nunik; Dany, Frans
Universa Medicina Vol. 40 No. 1 (2021)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2021.v40.3-13

Abstract

BackgroundType 2 diabetes mellitus (DM) is a non-communicable disease that constitutes a huge health burden, with the presence of comorbidities of DM adding to it. This study aimed to obtain the main determinants of the combined incidence of DM and its main comorbidities in adults.MethodsThis was a further analysis of the Non-Communicable Disease Risk Factor Cohort Study 2011 – 2018 involving 3730 subjects. Data of diabetes-free respondents at baseline were followed up every 2 years for 6 years. Data collection was carried out through interviews and health examinations. All subjects were assayed for blood glucose and lipid parameters. Chi-square test and Cox regression were implemented for data analysis.ResultsDuring 6 years of follow-up, DM incidence occurred in 567 (15.2%) subjects. The most common comorbidities were increased low density lipoprotein (LDL), central obesity, increased total cholesterol, obesity and hypertension. Most of the comorbidities occurred before the diagnosis of DM incidence. The determinants of the combined DM incidence–increased LDL are obesity, hypertension, and a family history of DM. The determinants of the combined DM incidence–central obesity are increased triglycerides, hypertension, male gender, and family history of DM. While the determinants of the combined DM incidence–hypertension are obesity and increased triglycerides.CONCLUSIONThis study demonstrated a high burden of diabetes incidence with comorbidities among adults. Knowledge of the magnitude of the diabetescomorbidity determinants emphasizes the role of non pharmacological intervention such as weight reduction and dietary modification.
Dyslipidemia in Renal Dysfunction among Non-diabetic Individuals from the 2019 Indonesian Cohort Study: A Cross-Sectional Study Retiaty, Fifi; Dany, Frans; Ernawati, Fitrah; Nurjanah, Nunung; Efriwati, Eriwati; Arifin, Aya Yuriestia; Sundari, Dian; Prihatini, Mutiara; Widoretno, Widoretno; Sahara, Ema; Imanningsih, Nelis; Herawati, Ade Nugraheni
Jurnal Gizi dan Pangan Vol. 18 No. 2 (2023)
Publisher : The Food and Nutrition Society of Indonesia in collaboration with the Department of Community Nutrition, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25182/jgp.2023.18.2.71-78

Abstract

The aim of this study was to investigate the relationship between dyslipidemia and the estimated Glomerular Filtration Rate (eGFR) values in a healthy population without a history of diabetes mellitus. Data were part of the cohort study database of 2019. Data analysis was perfomed using descriptive and inferential statistics with linear regression in 893 of 1,545 non-diabetic participants. The results showed that the average cholesterol levels, High-Density Lipoprotein (HDL), Low-Density Lipoprotein (LDL), and triglycerides were 196.75, 48.71, 123.37, and 109.56 mg/dl, respectively, and the average eGFR level of the respondents was 98.47±15.50 mg/dl. This study found that age, HDL levels, and LDL levels had a significant relationship with eGFR (p<0.05). It was concluded that increasing age and LDL levels and decreasing HDL levels would decrease eGFR.
Co-Authors - Pusat Biomedis dan Teknologi Dasar Kesehatan Balitbangkes, Kemenkes RI, Uly Alfi Nikmah Aya Yuriestia Arifin, Aya Yuriestia Dewi Kristanti Dian Sundari Efriwati, Eriwati Ekowati Rahajeng Ema Sahara Eva Sulistiowati Fifi Retiaty Fitrah Ernawati Handayani, Sarwo Herawati, Ade Nugraheni Nelis Imanningsih Nunik Kusumawardani Nunung Nurjanah Prihatini, Mutiara Ratih Rinendyaputri Widoretno, Widoretno