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All Journal Jurnal Teknologi Perikanan dan Kelautan Jurnal Ilmu dan Teknologi Kelautan Tropis Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Albacore : Jurnal Penelitian Perikanan Laut Bawal : Widya Riset Perikanan Tangkap Journal of Aquaculture and Fish Health Jurnal Ekologi, Masyarakat dan Sains Juvenil: Jurnal Ilmiah Kelautan dan Perikanan JURNAL SAINS DAN TEKNOLOGI PERIKANAN (JIKAN) Journal of Marine Studies
Sugeng Hartono
Marine Biology Department, Faculty Of Marine Science, King Abdulaziz University, Jeddah, Saudi Arabia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Engineering Environmental Science Library & Information Science Social Sciences

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KONFIRMASI INTEGRATIF MORFOLOGI DAN DNA BARCODING IKAN SEBELAH YANG KEKURANGAN DATA DI PERAIRAN CILACAP, INDONESIA: INTEGRATIVE MORPHOLOGICAL AND DNA BARCODING CONFIRMATION OF THE DATA-DEFICIENT INDIAN HALIBUT IN CILACAP WATERS, INDONESIA Hartono, Sugeng; Haryati, Ani; Salsabila, Sahda; Adiyanto, Fajar; Sukardi, Purnama; Puspito, Gondo; Abdulrahman, Idris
Jurnal Teknologi Perikanan dan Kelautan Vol. 17 No. 1 (2026): FEBRUARY 2026
Publisher : Fakultas Perikanan dan Ilmu Kelautan, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24319/jtpk.17.126-138

Abstract

The Indian halibut (Psettodes erumei) is classified as data deficient on the IUCN Red List. Accurate species identification is a fundamental prerequisite for sustainable fisheries management and biodiversity conservation. This study aims to identify the Indian halibut landed from Cilacap waters, Indonesia, using an integrative morphological and molecular approach. Four specimens collected in March and June 2025 were analyzed using an integrated morphological and molecular approach. Morphological identification was based on detailed morphometric and meristic analyses, cross-referenced with standard taxonomic guides. Molecular identification involved DNA extraction, PCR amplification of the mitochondrial Cytochrome C Oxidase Subunit I (COI) gene, electrophoresis, sequencing, and comparison with global genetic databases using BLAST. Morphological characteristics aligned with the description of P. erumei, though significant morphometric differences from a historical reference suggest potential local phenotypic variation. DNA barcoding confirmed the identity with 100% sequence similarity to conspecifics from other Indo-Pacific regions (GenBank accession: PX599017.1). Phylogenetic analysis further placed the Cilacap samples within a genetically homogeneous cluster of P. erumei from across Indonesian waters. This research successfully contributes to Indonesia's biodiversity database and DNA barcode library for a commercially exploited yet understudied species. It also provides essential baseline data to inform stock assessments and conservation strategies in the region.
Co-Authors Abdulrahman, Idris Abualreesh, Muyassar Hamid Adiyanto, Fajar Akther, Sajia Alim, Muhammad Bachrun Amir Yarkhasy Yuliardi Amran, Ramzi H. Andrew Amadeus Ani Haryati, Ani Ani Suryanti Aristi Dian Purnama Fitri Doan, Hien Van Etty Riani Fakhri Kurniawan Fredinan Yulianda Gabr, Mohamed Hosny Gondo Puspito Hanifa, Irfan Junaidi, Teuku Khan, Saadullah Jan Linh, Nguyen Vu Luhur Moekti Prayogo Muhammad Natsir Kholis Mustaruddin Nugroho, Agung Tri Purnama Sukardi Rahmalia, Diah Ayu Salsabila, Sahda Santanumurti, Muhammad Browijoyo Sari, Ratna Juita Sumon, Md Afsar Ahmed Wazir Mawardi