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All Journal CAUCHY: Jurnal Matematika Murni dan Aplikasi Journal of Tropical Biodiversity and Biotechnology REINWARDTIA BERITA BIOLOGI Biosaintifika: Journal of Biology & Biology Education Journal of Tropical Crop Science Imajiner: Jurnal Matematika dan Pendidikan Matematika Journal Mathematics Education Sigma [JMES] Buitenzorg: Journal of Tropical Science
DIAN LATIFAH, DIAN
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Other

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Pengaruh lama penyimpanan biji dan beberapa media perkecambahan terhadap daya kecambah biji Pinanga javana Helmanto, Hendra; Damayanti, Frisca; Yudaputra, Angga; Zulkarnaen, Rizmoon Nurul; Latifah, Dian
Buitenzorg: Journal of Tropical Science Vol 1 No 1 (2024): Buitenzorg: Journal of Tropical Science
Publisher : Innovation Centre for Tropical Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70158/buitenzorg.v1i1.3

Abstract

The IUCN red listed Pinanga javana Blume, also known as Javanese Pinang, as a protected and threatened species that is endemic to Java island, Indonesia. This research focused on study of the germination and growth process of P. javana seedlings using four types of planting media: cocopeat, husks, sand, and sawdust. The results showed that P. javana seed water content slowly decreased from 33.96% to 24.45% during the storage period from day 0 to day 21. The planting medium had a significant influence on the germination rate, with sand exhibiting the highest value (2.34) and husks providing the best seedling growth. Cocopeat had the highest moisture level (90%), while sand generated the lowest moisture level. The pH of all media was within the normal range (6-7), which supports optimal germination. The analysis of various parameters concluded that sand was the optimal planting medium for P. javana in this study. Keywords: germination, planting media, Pinanga javana, viability, water content
Cryopreservation of Terap (Artocarpus elasticus Reinw. ex. Blume) Seeds: Viability and Structural Characterization Wardani, Fitri Fatma; Efendi, Darda; Suhartanto, Mohamad Rahmad; Purwoko, Bambang Sapta; Latifah, Dian
Journal of Tropical Crop Science Vol. 13 No. 01 (2026): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.13.01.199-211

Abstract

Terap (Artocarpus elasticus Reinw. ex Blume) is an underutilized fruit tree native to Indonesia, valued both for its edible fruit and its diverse applications in traditional medicine. Terap seeds are recalcitrant, so cryopreservation is the most effective method for their long-term preservation. This study aimed to determine the optimal loading and vitrification times for cryopreservation to maintain seed viability. A factorial, completely randomized design was employed with two factors: loading time (0 and 20 min) and vitrification time (0, 30, and 60 min). Seed viability after loading and vitrification treatment with or without freezing in liquid nitrogen was evaluated using tetrazolium chloride (TTZ) and germination tests. Results indicated that the loading and vitrification treatments did not yet improve the viability of terap seeds after cryopreservation, as determined by the TTZ and germination tests. The TTZ test showed that the viability of all treated seeds decreased, regardless of whether they were frozen in liquid nitrogen. Germination tests revealed that all treated seeds without freezing had reduced germination, while none of the seeds treated with freezing germinated. Structural analysis indicated that freezing alters cell degradation, likely due to the formation of ice crystals and mechanical stress. To improve the success of terap seed cryopreservation, future efforts should focus on optimizing exposure times, reducing PVS2 toxicity, and enhancing cryoprotectant penetration by removing the seed coat or using the embryo axis as an explant.
Co-Authors Albania, Imam Nugraha Bambang Sapta Purwoko Budianti, Noviana Damayanti, Frisca Dian Rosleine, Dian Efendi, Darda Esti Munawaroh Fitri Fatma Wardani Fitri Kurniawati Hadiwiyono, Supandi Helmanto, Hendra Irsyam, Arifin Surya Dwipa JOSEPH A. HOLTUM, JOSEPH A. Mohamad Rahmad Suhartanto Muhammad Prayito Muhammad Rifqi Hariri Musyarofah Zuhri Noviady, Ikhsan Peniwidiyanti, Peniwidiyanti Purwantoro, RS Rahmaningtiyas, Lutfi Rasiman Rasiman Ria Windi Lestari Rizky Rosjanuardi ROBERT A. CONGDON, ROBERT A. Saripudin Saripudin Sri Hartini Sugiyono, Eko Suhaya, Yoyo Suhendri, Yudi Sulistyawati, Endah Sumanang Muhtar Gozali Wawangningrum, Hary Widjaya, Aulia Hasan Yudaputra, Angga Yuliyanto, Ponco Zulkarnaen, Rizmoon Nurul