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All Journal Dental Journal (Majalah Kedokteran Gigi) Jurnal Plastik Rekonstruksi JITEKGI Jurnal Ilmiah dan Teknologi Kedokteran Gigi Moestopo International Review on Social, Humanities, and Sciences (MIRSHuS) M-Dental Education and Research Journal
Evie Lamtiur Pakpahan
Universitas Prof. Dr. Moestopo (Beragama), Jakarta
Humanities Civil Engineering, Building, Construction & Architecture Dentistry Education Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Public Health Social Sciences Other

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TOXICITY TEST OF ROSELLE CALYX EXTRACT ON OSTEOBLASTS CELLS USING MTT ASSAY Pakpahan, Evie Lamtiur
Moestopo International Review on Social, Humanities, and Sciences Vol. 5 No. 2 (2025)
Publisher : Universitas prof. Dr. Moestopo (Beragama)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32509/mirshus.v5i2.112

Abstract

Background: Roselle calyx are the part of the roselle plant that is most often used as an extract because it has a lot of bioactive content. Roselle calyx contain dietary fiber, polyphenols, vitamin C, iron, zinc, calcium, magnesium, and potassium, often used as raw materials for herbal medicines. Polyphenols as a whole also have benefits for bone remodeling. Polyphenols can maintain bone integrity by reducing oxidative stress, reducing inflammation through signaling pro-inflammatory and modulation of osteoblastogenesis or osteoclastogenesis through osteoimmunological actions. Methods: Cytotoxicity was evaluated using MTT assay, and data were analyzed using the Shapiro-Wilk normality followed by one-way ANOVA and Tukey’s HSD post hoc test. Results: The result showed that osteoblast cell viability after treatment with roselle calyx extract was 93.95% at 15%, 99.60% at 20%, 108.87% at 25%, and a concentration of 30% had the highest cell viability percentage of 136.69%. Conclusion: Roselle calyx extract is non-toxic to osteoblast cells and has the potential as an alternative natural material to inhibit relapse in orthodontic treatment.
TOXICITY TEST OF ROSELLE NANOHYDROXYAPATITE-GELATIN ON OSTEOBLASTS CELLS USING MTT ASSAY Pakpahan, Evie Lamtiur
Moestopo International Review on Social, Humanities, and Sciences Vol. 5 No. 2 (2025)
Publisher : Universitas prof. Dr. Moestopo (Beragama)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32509/mirshus.v5i2.118

Abstract

Background: Orthodontic treatment often results in a relapse of tooth position after appliance removal due to insufficient bone remodeling. Effective prevention requires controlling inflammation and enhancing osteoblast activity to promote new bone formation and stabilization. Roselle calyx extract contains flavonoids with anti-inflammatory and antioxidant properties, while nanohydroxyapatite combined with gelatin improves scaffold porosity, cell adhesion, and biodegradability, thereby enhancing bone bioactivity. Methods: This in vitro study evaluated the cytocompatibility of nanohydroxyapatite–gelatin–roselle (NanoHAp-Gel-RE) composite on osteoblasts using an MTT assay. Data were analyzed for normality using the Shapiro–Wilk test, followed by one-way ANOVA and Tukey’s HSD post hoc test to determine differences between treatment groups. Results: Cell viability analysis showed that NanoHAp-Gel-RE at concentrations of 15%, 20%, 25%, and 30% resulted in viability values of 90.71%, 112.38%, 117.96%, and 117.34%, respectively. All tested concentrations demonstrated viability greater than 50%, indicating no cytotoxic effects. Conclusion: Roselle calyx extract and NanoHAp-Gel-RE composite exhibit good cytocompatibility toward osteoblast cells. Their ability to enhance cell viability suggests the potential for application as biocompatible therapeutic materials to support bone remodeling in preventing post-orthodontic relapse.
Co-Authors Akifah, Tasha Farah Andini, Shasya Putri Aprilia, Tasya Arief, Ridzky Auliadini, Sabrina Fitri Aurellia, Syifa Callista Bangun, Kristaninta Cahyaningrum, Firdayani Agnes Citraresmi , Siti Annisa Dermawan, Tara Marie Erindra, Shafrina Farida, Riska Fransisca, Tiara Fredina, Finda Hermawan, Laras Meisitha Indriani, Rezka Indriani Karauwan, Sharron Imanuela Kusparmanto, Farhany Sefina Kusparmanto, Lukas Nabilahsari, Thasia Putri Pandelaki, Refky V. Poppy Amelia Putri, Sekar Riandani Rajagukguk, Kezia Revi RR. Ella Evrita Hestiandari Sagita, Kintan Ratu Salsabila Wardhana, Safina Sandhi, Afriyanti Siti Handayani Sudarkirana, Sultan Nuur Sumarno, Muhammad Falih Syafiq, Mochammad Syauqi Taqy, Muhammad Uno, Salsabila Putri Winaprilia, Salsabila Sri Wirapradina, Revadya Yuditha, Solva Yusuf, Fika Maziya