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Pendampingan Pelestari Anggrek melalui Program Adopsi dan Pengembalian Vanda tricolor di Lereng Gunung Merapi Rineksane, Innaka Ageng; Astuti, Agung; Samidjo, Gatot Supangkat; Wulandari, Rani Agustina; Putri, Adinda Anisa
Unri Conference Series: Community Engagement Vol 6 (2024): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.6.781-785

Abstract

The Merapi orchid conservation farmer group is in Turgo, Purwobinangun, Sleman, Yogyakarta. This agricultural collective preserves the Merapi orchid and reintroduces it to its native environment on the slopes of Mount Merapi. Vanda tricolor is an orchid species that requires conservation owing to its declining population, attributed to recurrent eruptions and the exploitation of its native habitat. This adoption initiative necessitates expertise and financial resources; thus, support for farmer groups is essential. The proposed method involves counseling and support for farmers. This program aims to enhance the knowledge and skills of farmer group members and orchid conservation advocates in the adoption and reintroduction of Vanda tricolor orchids to their native habitat. This service is implemented by counseling, facilitating equipment and technology transfer, aiding in adopting and returning orchids, and conducting monitoring and evaluation. The event was attended by 5 men and 8 women, all members of the farmer group and supporters of orchid conservation in Merapi. Three adult Vanda tricolor specimens have been reattached to the puspa tree on the slopes of Mount Merapi. The pre-and post-test analysis results indicated that participants' knowledge and comprehension of the adoption and return of Merapi orchids improved from 27.9% to 100% following direct explanations and practical experience.
The Effect of Sample Weigh, Incubation Duration And Isolation Methods on The Quality of Phalaeonopsis amabilis root DNA Handayani, Etty; Rineksane, Innaka Ageng; Isnawan, Bambang Heri; Setiawan, Haris Anggita; Manto, Agus
Tropical Plantation Journal Vol 4, No 2 (2025): TROPICAL PLANTATION JOURNAL
Publisher : Akademi Komunitas Perkebunan Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56125/tpj.v4i2.63

Abstract

Orchid Ph. amabilis is one of the plants designated as "Indonesian National Flower" and designated as "Puspa Pesona Indonesia". Orchid Ph. amabilis has a diversity that is not widely known, so that it is necessary to identify it molecularly. DNA isolation is the first step in molecular testing. This study aims to determine the sample weight, incubation time and the appropriate isolation method for DNA isolation from samples of Ph.amabilis orchid roots. In this study, the DNA isolation stage used two experimental methods, namely the CTAB method (Murray and Thompson) and the mini kit. The research method used was a single factor experiment with a combination of sample weight and incubation time consisting of 6 treatments with 3 replications, so 18 experimental units were obtained. The experimental treatment used was from the roots of the orchid Ph. amabilis which has a sample weight of 0.25 g, 0.5 g, and 0.75 g and each sample weight treatment was incubated for 30 minutes and 60 minutes. In addition, DNA isolation using the kit method had a sample weight of 0.25 g, 0.5 g and 0.75 g and an incubation period of 10 minutes. The results showed that the DNA isolation of Ph. amabilis with a sample weight of 0.75g, incubation time of 30 minutes using the CTAB method showed the best treatment in producing DNA with a concentration of 384 ng/µl and a purity of 1.9. This study is expected to provide information on sample weight, incubation time and the appropriate method for DNA isolation of Ph. amabilis orchid roots.
Pengenalan Pertanian Modern pada Siswa SMA 1 Godean melalui Pelatihan Kultur Jaringan Tanaman: Introduction to Modern Agriculture for SMA 1 Godean Students through Plant Tissue Culture Training Rineksane, Innaka Ageng; Handayani, Etty; Sari, Supriyatin Kartika; Wulandari, Rani Agustina
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 11 No. Suppl-1 (2026): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v11iSuppl-1.10280

Abstract

SMA Negeri 1 Godean, Sleman, Yogyakarta, has achieved significant academic success, including higher graduation rates and a consistent ranking among the top five high schools in Sleman Regency. This institution is recognized for its students' adherence to established rules and regulations. Consequently, the school aims to enhance its students' understanding by including agriculture, in partnership with the Faculty of Agriculture at Universitas Muhammadiyah Yogyakarta. SMA Negeri 1 Godean is equipped with Biology and Chemistry laboratories, along with a greenhouse, to facilitate the introduction and exploration of scientific disciplines. However, the institution lacks the facilities to implement modern agricultural techniques for its students. A modern agrarian approach is the plant tissue culture technology. The challenge at present is that the students of SMA Negeri 1 Godean lack an understanding of agriculture, both in general and in its modern form, and have not had practical experience with plant tissue culture. This service aims to familiarize SMA Negeri 1 Godean students with the science of agriculture and modern agricultural techniques through tissue culture. Efforts to address the partner's issues were implemented through various methods, including general and modern agricultural education, specifically plant tissue culture techniques; practical training in tissue culture methods; the acclimatization of Cavendish bananas; and evaluation. The results of the activities indicate an improvement in students' knowledge and skills following their participation in general and modern agricultural culture, especially in plant tissue culture techniques.
Substitution Media by Utilizing Banana Peel for In Vitro Culture of Chrysanthemum morifolium Handayani, Etty; Rineksane, Innaka Ageng; Sari, Supriyatin Kartika; Permatasari, Fany
Tropical Plantation Journal Vol 5, No 1 (2026): TROPICAL PLANTATION JOURNAL
Publisher : Akademi Komunitas Perkebunan Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56125/tpj.v5i1.68

Abstract

The use of organic matter in the in vitro culture medium can reduce costs for micropropagation and multiplication of Chrysanthemum in mass production. This study aims to examine the use of banana peel waste in the in vitro culture of Chrysanthemum. The research used an experimental method with single factor and eight treatments with 3 samples and 3 replications. The treatments were: (1) MS medium +  BAP 1 ppm + NAA 0.5 ppm, (2) Foliar fertilizer 3 g/L +  coconut water 150 mL/L, (3) banana endocarp puree 50 g/L, (4)  banana endocarp puree 100 g/L, (5)  banana exocarp puree 50 g/L, (6) banana exocarp puree 100 g/L, (7) banana peel puree 100 g/L, and (8) banana peel puree 100 g/L. All banana peel puree treatments were combined with foliar fertilizer 3g/L + coconut water 150 mL/L. The explant used a single nodus of Chrysanthemum with one leaf resulting from the previous in vitro culture. This study used Chrysanthemum varieties Naweswari and Tadasita from Ornamental Plants Research Center, West Java, Indonesia. The results showed that foliar fertilizer medium added by banana peel puree and coconut water has the good potential to be used as a substitution medium in the in vitro culture of Chrysanthemum varieties Naweswari and Tadasita. The banana peel puree  50 g/L in the foliar fertilizer medium shows the best growth in Chrysanthemum in vitro culture in both Naweswari and Tadasita varieties.