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All Journal Vegetalika Planta Tropika BERDIKARI : Jurnal Inovasi dan Penerapan Ipteks BIO-EDU: Jurnal Pendidikan Biologi Unri Conference Series: Community Engagement Prosiding Seminar Nasional Program Pengabdian Masyarakat Tropical Plantation Journal
Innaka Ageng Rineksane
Universitas Muhammadiyah Yogyakarta
Agriculture, Biological Sciences & Forestry Humanities Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Economics, Econometrics & Finance Education Medicine & Pharmacology Social Sciences

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Pendampingan Pelestari Anggrek melalui Program Adopsi dan Pengembalian Vanda tricolor di Lereng Gunung Merapi Rineksane, Innaka Ageng; Astuti, Agung; Samidjo, Gatot Supangkat; Wulandari, Rani Agustina; Putri, Adinda Anisa
Unri Conference Series: Community Engagement Vol 6 (2024): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.6.781-785

Abstract

The Merapi orchid conservation farmer group is in Turgo, Purwobinangun, Sleman, Yogyakarta. This agricultural collective preserves the Merapi orchid and reintroduces it to its native environment on the slopes of Mount Merapi. Vanda tricolor is an orchid species that requires conservation owing to its declining population, attributed to recurrent eruptions and the exploitation of its native habitat. This adoption initiative necessitates expertise and financial resources; thus, support for farmer groups is essential. The proposed method involves counseling and support for farmers. This program aims to enhance the knowledge and skills of farmer group members and orchid conservation advocates in the adoption and reintroduction of Vanda tricolor orchids to their native habitat. This service is implemented by counseling, facilitating equipment and technology transfer, aiding in adopting and returning orchids, and conducting monitoring and evaluation. The event was attended by 5 men and 8 women, all members of the farmer group and supporters of orchid conservation in Merapi. Three adult Vanda tricolor specimens have been reattached to the puspa tree on the slopes of Mount Merapi. The pre-and post-test analysis results indicated that participants' knowledge and comprehension of the adoption and return of Merapi orchids improved from 27.9% to 100% following direct explanations and practical experience.
The Effect of Sample Weigh, Incubation Duration And Isolation Methods on The Quality of Phalaeonopsis amabilis root DNA Handayani, Etty; Rineksane, Innaka Ageng; Isnawan, Bambang Heri; Setiawan, Haris Anggita; Manto, Agus
Tropical Plantation Journal Vol 4, No 2 (2025): TROPICAL PLANTATION JOURNAL
Publisher : Akademi Komunitas Perkebunan Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56125/tpj.v4i2.63

Abstract

Orchid Ph. amabilis is one of the plants designated as "Indonesian National Flower" and designated as "Puspa Pesona Indonesia". Orchid Ph. amabilis has a diversity that is not widely known, so that it is necessary to identify it molecularly. DNA isolation is the first step in molecular testing. This study aims to determine the sample weight, incubation time and the appropriate isolation method for DNA isolation from samples of Ph.amabilis orchid roots. In this study, the DNA isolation stage used two experimental methods, namely the CTAB method (Murray and Thompson) and the mini kit. The research method used was a single factor experiment with a combination of sample weight and incubation time consisting of 6 treatments with 3 replications, so 18 experimental units were obtained. The experimental treatment used was from the roots of the orchid Ph. amabilis which has a sample weight of 0.25 g, 0.5 g, and 0.75 g and each sample weight treatment was incubated for 30 minutes and 60 minutes. In addition, DNA isolation using the kit method had a sample weight of 0.25 g, 0.5 g and 0.75 g and an incubation period of 10 minutes. The results showed that the DNA isolation of Ph. amabilis with a sample weight of 0.75g, incubation time of 30 minutes using the CTAB method showed the best treatment in producing DNA with a concentration of 384 ng/µl and a purity of 1.9. This study is expected to provide information on sample weight, incubation time and the appropriate method for DNA isolation of Ph. amabilis orchid roots.
Co-Authors Agung Astuti Alawiyah, Riffa Leshia Muhvi Nur Alfaris, Muhammad Rizal Aris, Irfan Ayuningtias, Nandini Bambang Heri Isnawan Dewi, Sukuriyati Susilo Etty Handayani Genesiska, Genesiska Handayani, Etty Indira Prabasari, Indira Irsyadi, Muhammad Burhanuddin Jamhari Jamhari Kristianto Nugroho Manto, Agus Nafiah, Siti Safitri Pamuji, Kurniawati Permatasari, Fany Pramula, Faqikh Gesang Pranata, Arya Eka Puji Lestari Putri, Adinda Anisa Rani Agustina Wulandari Rani Agustina Wulandari, Rani Agustina Rerenstradika Tizar Terryana Rizki Eka Putri Rustiningsih Rustiningsih Setiawan, Haris Anggita Shanti Wardaningsih Siti Nur Aisyah Siti Safitri Nafiah Supangkat, Gatot Utama, Nafi Ananda